>KMC016425A_C01 KMC016425A_c01
agatggTAGTGGTCATATCACGGTTAAGTTCATACGAAAACCTCTTAAAAGGAGTACAGC
AATCAGGGCAAAGCTCATAACTGAACTTACAAGAGAAAACAAACACTAGCCAACACGGCC
CTCACCTAACCAACACCACCACCCTCTAAAAAGAAAAAGAAAAAAAAAGAAAAAACCCTA
AAGGTCAACAATAGCAAAGGGCATCAGAAGCCATTCCCACTAAGGTTCAGAGATAATCCT
AACAAACTCCATGGAAGTTAGTACAGAGTCAACAGACACCATAAGGCACAAAATTGGCGA
ACGGTACAAGGGAAATCCCAGGTCCCACGAGCTCTTTAGTTTAATGCCAATCAGTTCCAA
TTGGCAAGCTTGAATGATCAAGCAAGCAAGCCTGAAAAAAATTAGCAAAATGAAGCCACC
ATTTAAGTGGATACTTACCAGTA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC016425A_C01 KMC016425A_c01
         (443 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_013370.1| Synaptobrevin (v-SNARE) homolog present on ER t...    34  0.36
ref|NP_067266.1| glycoprotein-associated amino acid transporter ...    33  0.61
dbj|BAC35548.1| unnamed protein product [Mus musculus]                 33  1.0
gb|AAH28246.1| RIKEN cDNA 2310005B10 gene [Mus musculus]               33  1.0
ref|NP_080136.1| RIKEN cDNA 2310005B10 [Mus musculus] gi|1286020...    33  1.0

>ref|NP_013370.1| Synaptobrevin (v-SNARE) homolog present on ER to Golgi vesicles;
           Sec22p [Saccharomyces cerevisiae]
           gi|134542|sp|P22214|SC22_YEAST Protein transport protein
           SEC22 (Protein SLY2) gi|1078357|pir||S51405
           synaptobrevin SEC22 - yeast (Saccharomyces cerevisiae)
           gi|4486|emb|CAA38142.1| Sly2p [Saccharomyces cerevisiae]
           gi|577174|gb|AAB67373.1| Sec22p: synaptobrevin (v-SNARE)
           homolog [Saccharomyces cerevisiae]
          Length = 214

 Score = 34.3 bits (77), Expect = 0.36
 Identities = 25/79 (31%), Positives = 40/79 (49%), Gaps = 2/79 (2%)
 Frame = -3

Query: 357 ELIGIKLKSSWDLGFPLYRSPILCLMVSVDSVL--TSMEFVRIISEP*WEWLLMPFAIVD 184
           EL+G+K   S ++   LYR   L  M  + S L  TS  + +   +  ++ L+  +A + 
Sbjct: 139 ELVGVKQIMSKNIEDLLYRGDSLDKMSDMSSSLKETSKRYRKSAQKINFDLLISQYAPIV 198

Query: 183 L*GFFFFFLFLFRGWWCWL 127
           +  FFF FLF    WW +L
Sbjct: 199 IVAFFFVFLF----WWIFL 213

>ref|NP_067266.1| glycoprotein-associated amino acid transporter  b0,+AT1; b, +AT;
           b, + amino acid transporter [Mus musculus]
           gi|12585193|sp|Q9QXA6|BAT1_MOUSE B(0,+)-type amino acid
           transporter 1 (B(0,+)AT) (Glycoprotein-associated amino
           acid transporter b0,+AT1) gi|5824165|emb|CAB54042.1|
           glycoprotein-associated amino acid transporter b0,+AT1
           [Mus musculus] gi|11065931|gb|AAG28396.1|AF192310_1
           amino acid transporter subunit b0,+AT [Mus musculus]
           gi|14789621|gb|AAH10746.1| solute carrier family 7
           (cationic amino acid transporter, y+ system), member 9
           [Mus musculus]
          Length = 487

 Score = 33.5 bits (75), Expect = 0.61
 Identities = 21/55 (38%), Positives = 31/55 (56%), Gaps = 2/55 (3%)
 Frame = -3

Query: 297 PILCLMVSVDSVLTSMEFVRIISEP*WEWLLMPFAIVDL*GFFFFFLFLFR--GW 139
           PI+ ++VS+  +L       IISEP WE+L     I+   G  F+FLF++   GW
Sbjct: 414 PIIVILVSLFLILAP-----IISEPAWEYLYCVLFILS--GLIFYFLFVYYKFGW 461

>dbj|BAC35548.1| unnamed protein product [Mus musculus]
          Length = 608

 Score = 32.7 bits (73), Expect = 1.0
 Identities = 20/55 (36%), Positives = 27/55 (48%), Gaps = 5/55 (9%)
 Frame = +1

Query: 130 PTPPPSKK-KKKKKEKTLKVNNSKGHQKPFPLRFR----DNPNKLHGS*YRVNRH 279
           PTPPPS + KKKKK+ T K         P P++ +       +K H S  +  RH
Sbjct: 100 PTPPPSSRGKKKKKKSTRKKRRRSPSYSPSPVKKKKKKSSKKHKRHRSFSKKRRH 154

>gb|AAH28246.1| RIKEN cDNA 2310005B10 gene [Mus musculus]
          Length = 660

 Score = 32.7 bits (73), Expect = 1.0
 Identities = 17/33 (51%), Positives = 20/33 (60%)
 Frame = +1

Query: 103 TLANTALT*PTPPPSKKKKKKKEKTLKVNNSKG 201
           TLAN     PTPPP  + KKKK+K  K+ N  G
Sbjct: 92  TLANGEAA-PTPPPDSEVKKKKKKKRKMANDAG 123

>ref|NP_080136.1| RIKEN cDNA 2310005B10 [Mus musculus] gi|12860207|dbj|BAB31877.1|
           unnamed protein product [Mus musculus]
          Length = 660

 Score = 32.7 bits (73), Expect = 1.0
 Identities = 17/33 (51%), Positives = 20/33 (60%)
 Frame = +1

Query: 103 TLANTALT*PTPPPSKKKKKKKEKTLKVNNSKG 201
           TLAN     PTPPP  + KKKK+K  K+ N  G
Sbjct: 92  TLANGEAA-PTPPPDSEVKKKKKKKRKMANDAG 123

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 379,544,135
Number of Sequences: 1393205
Number of extensions: 8212496
Number of successful extensions: 32789
Number of sequences better than 10.0: 86
Number of HSP's better than 10.0 without gapping: 25965
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 30553
length of database: 448,689,247
effective HSP length: 123
effective length of database: 277,325,032
effective search space used: 6655800768
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 SPDL045e11_f BP054831 1 443 2 SPDL001c11_f BP052038 7 327

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