Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC016153A_C01 KMC016153A_c01
(497 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAL32543.1| Unknown protein [Arabidopsis thaliana] gi|2319782... 41 0.006
pir||A34092 DNA-directed RNA polymerase (EC 2.7.7.6) II large ch... 38 0.055
ref|NP_500523.1| RNA polymerase II, AMAnitin resistant AMA-1 (am... 38 0.055
pir||T29959 DNA-directed RNA polymerase II large subunit ama-1 -... 38 0.055
pir||T31435 DNA-directed RNA polymerase (EC 2.7.7.6) II largest ... 37 0.12
>gb|AAL32543.1| Unknown protein [Arabidopsis thaliana] gi|23197822|gb|AAN15438.1|
Unknown protein [Arabidopsis thaliana]
Length = 563
Score = 41.2 bits (95), Expect = 0.006
Identities = 32/88 (36%), Positives = 42/88 (47%), Gaps = 8/88 (9%)
Frame = -2
Query: 475 ISPLPRED--------VASTKSGTSGTSSPLGFNSSPSRFKPSSPLSSPFKGRYNPDRPL 320
+SPLPRED + S+ SG SSP SPSRF +K ++P+
Sbjct: 493 VSPLPREDGVFDPRFGLGSSPKNISGASSP-----SPSRF---------WKEAHSPNVQT 538
Query: 319 SSVTEEVPEKAKDGVMNPPTPPSIHRES 236
EEV D + PP+P SIHRE+
Sbjct: 539 PREKEEVKS---DVALTPPSPLSIHREA 563
>pir||A34092 DNA-directed RNA polymerase (EC 2.7.7.6) II large chain -
Caenorhabditis elegans gi|156405|gb|AAA28126.1| RNA
polymerase II
Length = 1859
Score = 38.1 bits (87), Expect = 0.055
Identities = 29/98 (29%), Positives = 38/98 (38%), Gaps = 10/98 (10%)
Frame = -2
Query: 496 YADDQPQISPL-----PREDVASTKSGTSGTSSPLGFNSSPSRFKPSSPLSSPFKGRYNP 332
Y+ PQ SP P + S T +SP GF+S ++ P+SP SP Y P
Sbjct: 1744 YSPTSPQYSPTSPQYSPSSPTYTPSSPTYNPTSPRGFSSP--QYSPTSPTYSPTSPSYTP 1801
Query: 331 DRPLSSVTEEV-----PEKAKDGVMNPPTPPSIHRESP 233
P S T E+ PT P+ SP
Sbjct: 1802 SSPQYSPTSPTYTPSPSEQPGTSAQYSPTSPTYSPSSP 1839
Score = 38.1 bits (87), Expect = 0.055
Identities = 29/96 (30%), Positives = 41/96 (42%), Gaps = 8/96 (8%)
Frame = -2
Query: 496 YADDQPQISPLPREDVASTKSGTSGTSSPL--------GFNSSPSRFKPSSPLSSPFKGR 341
Y+ P SP + T S TS T SP G++ S ++ PSSP SP
Sbjct: 1689 YSPTSPTYSP-----TSPTYSPTSPTYSPTSPSYESGGGYSPSSPKYSPSSPTYSPTSPS 1743
Query: 340 YNPDRPLSSVTEEVPEKAKDGVMNPPTPPSIHRESP 233
Y+P P S T P+ + P+ P+ + SP
Sbjct: 1744 YSPTSPQYSPTS--PQYSPSSPTYTPSSPTYNPTSP 1777
Score = 34.3 bits (77), Expect = 0.79
Identities = 26/88 (29%), Positives = 34/88 (38%)
Frame = -2
Query: 496 YADDQPQISPLPREDVASTKSGTSGTSSPLGFNSSPSRFKPSSPLSSPFKGRYNPDRPLS 317
Y+ PQ S + + T S TS G + + P+SP SP Y+P P
Sbjct: 1582 YSPSSPQFS-MTSPHYSPTSPSYSPTSPAAGQSPVSPSYSPTSPSYSPTSPSYSPTSPSY 1640
Query: 316 SVTEEVPEKAKDGVMNPPTPPSIHRESP 233
S T P + PT PS SP
Sbjct: 1641 SPTS--PSYSPTSPSYSPTSPSYSPSSP 1666
Score = 34.3 bits (77), Expect = 0.79
Identities = 32/101 (31%), Positives = 39/101 (37%), Gaps = 13/101 (12%)
Frame = -2
Query: 496 YADDQPQISPL-----PREDVASTKSGTSGTSSPLGFNSSPSRFKPSSPLSSPFKGRYNP 332
Y+ P SP P S S + SSP SSP R+ P+SP SP Y+P
Sbjct: 1640 YSPTSPSYSPTSPSYSPTSPSYSPSSPSYSPSSPSYSPSSP-RYSPTSPTYSPTSPTYSP 1698
Query: 331 DRPLSSVTEEV-----PEKAKDGVMNPPTP---PSIHRESP 233
P S T P G +P +P PS SP
Sbjct: 1699 TSPTYSPTSPTYSPTSPSYESGGGYSPSSPKYSPSSPTYSP 1739
Score = 32.7 bits (73), Expect = 2.3
Identities = 20/71 (28%), Positives = 31/71 (43%)
Frame = -2
Query: 445 STKSGTSGTSSPLGFNSSPSRFKPSSPLSSPFKGRYNPDRPLSSVTEEVPEKAKDGVMNP 266
S ++ + G SP ++ S +F +SP SP Y+P P + + P +
Sbjct: 1568 SPRTPSYGGMSPGVYSPSSPQFSMTSPHYSPTSPSYSPTSPAAGQSPVSPSYSPTSPSYS 1627
Query: 265 PTPPSIHRESP 233
PT PS SP
Sbjct: 1628 PTSPSYSPTSP 1638
Score = 31.6 bits (70), Expect = 5.1
Identities = 21/58 (36%), Positives = 28/58 (48%)
Frame = -2
Query: 496 YADDQPQISPLPREDVASTKSGTSGTSSPLGFNSSPSRFKPSSPLSSPFKGRYNPDRP 323
Y+ P +P P E + GTS SP +SP+ + PSSP SP Y+P P
Sbjct: 1806 YSPTSPTYTPSPSE-----QPGTSAQYSP----TSPT-YSPSSPTYSPASPSYSPSSP 1853
>ref|NP_500523.1| RNA polymerase II, AMAnitin resistant AMA-1 (ama-1) [Caenorhabditis
elegans] gi|21542435|sp|P16356|RPB1_CAEEL DNA-directed
RNA polymerase II largest subunit
gi|14574184|gb|AAA96158.2| Amanitin resistant protein 1
[Caenorhabditis elegans]
Length = 1852
Score = 38.1 bits (87), Expect = 0.055
Identities = 29/98 (29%), Positives = 38/98 (38%), Gaps = 10/98 (10%)
Frame = -2
Query: 496 YADDQPQISPL-----PREDVASTKSGTSGTSSPLGFNSSPSRFKPSSPLSSPFKGRYNP 332
Y+ PQ SP P + S T +SP GF+S ++ P+SP SP Y P
Sbjct: 1737 YSPTSPQYSPTSPQYSPSSPTYTPSSPTYNPTSPRGFSSP--QYSPTSPTYSPTSPSYTP 1794
Query: 331 DRPLSSVTEEV-----PEKAKDGVMNPPTPPSIHRESP 233
P S T E+ PT P+ SP
Sbjct: 1795 SSPQYSPTSPTYTPSPSEQPGTSAQYSPTSPTYSPSSP 1832
Score = 38.1 bits (87), Expect = 0.055
Identities = 29/96 (30%), Positives = 41/96 (42%), Gaps = 8/96 (8%)
Frame = -2
Query: 496 YADDQPQISPLPREDVASTKSGTSGTSSPL--------GFNSSPSRFKPSSPLSSPFKGR 341
Y+ P SP + T S TS T SP G++ S ++ PSSP SP
Sbjct: 1682 YSPTSPTYSP-----TSPTYSPTSPTYSPTSPSYESGGGYSPSSPKYSPSSPTYSPTSPS 1736
Query: 340 YNPDRPLSSVTEEVPEKAKDGVMNPPTPPSIHRESP 233
Y+P P S T P+ + P+ P+ + SP
Sbjct: 1737 YSPTSPQYSPTS--PQYSPSSPTYTPSSPTYNPTSP 1770
Score = 34.3 bits (77), Expect = 0.79
Identities = 26/88 (29%), Positives = 34/88 (38%)
Frame = -2
Query: 496 YADDQPQISPLPREDVASTKSGTSGTSSPLGFNSSPSRFKPSSPLSSPFKGRYNPDRPLS 317
Y+ PQ S + + T S TS G + + P+SP SP Y+P P
Sbjct: 1575 YSPSSPQFS-MTSPHYSPTSPSYSPTSPAAGQSPVSPSYSPTSPSYSPTSPSYSPTSPSY 1633
Query: 316 SVTEEVPEKAKDGVMNPPTPPSIHRESP 233
S T P + PT PS SP
Sbjct: 1634 SPTS--PSYSPTSPSYSPTSPSYSPSSP 1659
Score = 34.3 bits (77), Expect = 0.79
Identities = 32/101 (31%), Positives = 39/101 (37%), Gaps = 13/101 (12%)
Frame = -2
Query: 496 YADDQPQISPL-----PREDVASTKSGTSGTSSPLGFNSSPSRFKPSSPLSSPFKGRYNP 332
Y+ P SP P S S + SSP SSP R+ P+SP SP Y+P
Sbjct: 1633 YSPTSPSYSPTSPSYSPTSPSYSPSSPSYSPSSPSYSPSSP-RYSPTSPTYSPTSPTYSP 1691
Query: 331 DRPLSSVTEEV-----PEKAKDGVMNPPTP---PSIHRESP 233
P S T P G +P +P PS SP
Sbjct: 1692 TSPTYSPTSPTYSPTSPSYESGGGYSPSSPKYSPSSPTYSP 1732
Score = 32.7 bits (73), Expect = 2.3
Identities = 20/71 (28%), Positives = 31/71 (43%)
Frame = -2
Query: 445 STKSGTSGTSSPLGFNSSPSRFKPSSPLSSPFKGRYNPDRPLSSVTEEVPEKAKDGVMNP 266
S ++ + G SP ++ S +F +SP SP Y+P P + + P +
Sbjct: 1561 SPRTPSYGGMSPGVYSPSSPQFSMTSPHYSPTSPSYSPTSPAAGQSPVSPSYSPTSPSYS 1620
Query: 265 PTPPSIHRESP 233
PT PS SP
Sbjct: 1621 PTSPSYSPTSP 1631
Score = 31.6 bits (70), Expect = 5.1
Identities = 21/58 (36%), Positives = 28/58 (48%)
Frame = -2
Query: 496 YADDQPQISPLPREDVASTKSGTSGTSSPLGFNSSPSRFKPSSPLSSPFKGRYNPDRP 323
Y+ P +P P E + GTS SP +SP+ + PSSP SP Y+P P
Sbjct: 1799 YSPTSPTYTPSPSE-----QPGTSAQYSP----TSPT-YSPSSPTYSPASPSYSPSSP 1846
>pir||T29959 DNA-directed RNA polymerase II large subunit ama-1 - Caenorhabditis
elegans
Length = 1862
Score = 38.1 bits (87), Expect = 0.055
Identities = 29/98 (29%), Positives = 38/98 (38%), Gaps = 10/98 (10%)
Frame = -2
Query: 496 YADDQPQISPL-----PREDVASTKSGTSGTSSPLGFNSSPSRFKPSSPLSSPFKGRYNP 332
Y+ PQ SP P + S T +SP GF+S ++ P+SP SP Y P
Sbjct: 1747 YSPTSPQYSPTSPQYSPSSPTYTPSSPTYNPTSPRGFSSP--QYSPTSPTYSPTSPSYTP 1804
Query: 331 DRPLSSVTEEV-----PEKAKDGVMNPPTPPSIHRESP 233
P S T E+ PT P+ SP
Sbjct: 1805 SSPQYSPTSPTYTPSPSEQPGTSAQYSPTSPTYSPSSP 1842
Score = 38.1 bits (87), Expect = 0.055
Identities = 29/96 (30%), Positives = 41/96 (42%), Gaps = 8/96 (8%)
Frame = -2
Query: 496 YADDQPQISPLPREDVASTKSGTSGTSSPL--------GFNSSPSRFKPSSPLSSPFKGR 341
Y+ P SP + T S TS T SP G++ S ++ PSSP SP
Sbjct: 1692 YSPTSPTYSP-----TSPTYSPTSPTYSPTSPSYESGGGYSPSSPKYSPSSPTYSPTSPS 1746
Query: 340 YNPDRPLSSVTEEVPEKAKDGVMNPPTPPSIHRESP 233
Y+P P S T P+ + P+ P+ + SP
Sbjct: 1747 YSPTSPQYSPTS--PQYSPSSPTYTPSSPTYNPTSP 1780
Score = 34.3 bits (77), Expect = 0.79
Identities = 26/88 (29%), Positives = 34/88 (38%)
Frame = -2
Query: 496 YADDQPQISPLPREDVASTKSGTSGTSSPLGFNSSPSRFKPSSPLSSPFKGRYNPDRPLS 317
Y+ PQ S + + T S TS G + + P+SP SP Y+P P
Sbjct: 1585 YSPSSPQFS-MTSPHYSPTSPSYSPTSPAAGQSPVSPSYSPTSPSYSPTSPSYSPTSPSY 1643
Query: 316 SVTEEVPEKAKDGVMNPPTPPSIHRESP 233
S T P + PT PS SP
Sbjct: 1644 SPTS--PSYSPTSPSYSPTSPSYSPSSP 1669
Score = 34.3 bits (77), Expect = 0.79
Identities = 32/101 (31%), Positives = 39/101 (37%), Gaps = 13/101 (12%)
Frame = -2
Query: 496 YADDQPQISPL-----PREDVASTKSGTSGTSSPLGFNSSPSRFKPSSPLSSPFKGRYNP 332
Y+ P SP P S S + SSP SSP R+ P+SP SP Y+P
Sbjct: 1643 YSPTSPSYSPTSPSYSPTSPSYSPSSPSYSPSSPSYSPSSP-RYSPTSPTYSPTSPTYSP 1701
Query: 331 DRPLSSVTEEV-----PEKAKDGVMNPPTP---PSIHRESP 233
P S T P G +P +P PS SP
Sbjct: 1702 TSPTYSPTSPTYSPTSPSYESGGGYSPSSPKYSPSSPTYSP 1742
Score = 32.7 bits (73), Expect = 2.3
Identities = 20/71 (28%), Positives = 31/71 (43%)
Frame = -2
Query: 445 STKSGTSGTSSPLGFNSSPSRFKPSSPLSSPFKGRYNPDRPLSSVTEEVPEKAKDGVMNP 266
S ++ + G SP ++ S +F +SP SP Y+P P + + P +
Sbjct: 1571 SPRTPSYGGMSPGVYSPSSPQFSMTSPHYSPTSPSYSPTSPAAGQSPVSPSYSPTSPSYS 1630
Query: 265 PTPPSIHRESP 233
PT PS SP
Sbjct: 1631 PTSPSYSPTSP 1641
Score = 31.6 bits (70), Expect = 5.1
Identities = 21/58 (36%), Positives = 28/58 (48%)
Frame = -2
Query: 496 YADDQPQISPLPREDVASTKSGTSGTSSPLGFNSSPSRFKPSSPLSSPFKGRYNPDRP 323
Y+ P +P P E + GTS SP +SP+ + PSSP SP Y+P P
Sbjct: 1809 YSPTSPTYTPSPSE-----QPGTSAQYSP----TSPT-YSPSSPTYSPASPSYSPSSP 1856
>pir||T31435 DNA-directed RNA polymerase (EC 2.7.7.6) II largest chain -
Vairimorpha necatrix gi|4001824|gb|AAD12604.1| largest
subunit of RNA polymerase II; RPO21 [Vairimorpha
necatrix]
Length = 1605
Score = 37.0 bits (84), Expect = 0.12
Identities = 30/90 (33%), Positives = 37/90 (40%), Gaps = 2/90 (2%)
Frame = -2
Query: 496 YADDQPQISPLPREDVASTKSGTSGTSSPLGFNSSPSR--FKPSSPLSSPFKGRYNPDRP 323
Y+ P SP + T S TS T SP SP+ + P+SP SP Y+P P
Sbjct: 1467 YSPTSPTYSP-----TSPTYSPTSPTYSPTSPTYSPTSPTYSPTSPTYSPTSPSYSPTSP 1521
Query: 322 LSSVTEEVPEKAKDGVMNPPTPPSIHRESP 233
S T P + PT PS SP
Sbjct: 1522 SYSPTS--PSYSPTSPSYSPTSPSYSPTSP 1549
Score = 35.4 bits (80), Expect = 0.36
Identities = 29/90 (32%), Positives = 38/90 (42%), Gaps = 2/90 (2%)
Frame = -2
Query: 496 YADDQPQISPLPREDVASTKSGTSGTSSPLGFNSSPSR--FKPSSPLSSPFKGRYNPDRP 323
Y+ P SP + T S TS + SP + SP+ + P+SP SP Y+P P
Sbjct: 1495 YSPTSPTYSP-----TSPTYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSP 1549
Query: 322 LSSVTEEVPEKAKDGVMNPPTPPSIHRESP 233
S T P + PT PS SP
Sbjct: 1550 SYSPTS--PSYSPTSPSYSPTSPSYSPTSP 1577
Score = 31.6 bits (70), Expect = 5.1
Identities = 23/71 (32%), Positives = 33/71 (46%), Gaps = 2/71 (2%)
Frame = -2
Query: 496 YADDQPQISPLPREDVASTKSGTSGTSSPLGFNSSPSR--FKPSSPLSSPFKGRYNPDRP 323
Y+ P SP + + S TS + SP + SP+ + P+SP SP Y+P P
Sbjct: 1530 YSPTSPSYSP-----TSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSP 1584
Query: 322 LSSVTEEVPEK 290
S T + EK
Sbjct: 1585 SYSPTYDNDEK 1595
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 435,017,641
Number of Sequences: 1393205
Number of extensions: 9458784
Number of successful extensions: 33319
Number of sequences better than 10.0: 182
Number of HSP's better than 10.0 without gapping: 30204
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 32313
length of database: 448,689,247
effective HSP length: 114
effective length of database: 289,863,877
effective search space used: 14783057727
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
