Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC015946A_C01 KMC015946A_c01
(567 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_191192.1| N2,N2-dimethylguanosine tRNA methyltransferases... 144 8e-34
sp|Q9YDY7|TRM1_AERPE N(2),N(2)-dimethylguanosine tRNA methyltran... 57 2e-07
ref|NP_147490.1| N2,N2-dimethylguanosine tRNA methyltransferase ... 57 2e-07
pir||T39993 N2,N2-dimethylguanosine tRNA methyltransferase - fis... 56 3e-07
pir||T46565 tRNA (guanine-N2-)-methyltransferase (EC 2.1.1.32) [... 56 3e-07
>ref|NP_191192.1| N2,N2-dimethylguanosine tRNA methyltransferases-like protein;
protein id: At3g56330.1 [Arabidopsis thaliana]
gi|11358573|pir||T47750 N2,N2-dimethylguanosine tRNA
methyltransferases-like protein - Arabidopsis thaliana
gi|7572931|emb|CAB87432.1| N2, N2-dimethylguanosine tRNA
methyltransferases-like protein [Arabidopsis thaliana]
Length = 433
Score = 144 bits (363), Expect = 8e-34
Identities = 66/106 (62%), Positives = 88/106 (82%)
Frame = -3
Query: 565 PLHDAAYLTDMLNLAKQWGWIGCDGKDSLEKLINVMVDESDPKLPFGYIKLDEMASRAKI 386
PLHDA+Y+T+ML LAK+WGW+ L+KL+++M++ESDP+LP GY K+DEMASRAK+
Sbjct: 326 PLHDASYVTEMLELAKEWGWVSEGTGMDLDKLLSIMIEESDPRLPPGYTKMDEMASRAKM 385
Query: 385 NSPPLKTLMSAIQQKGYAASRSHIKTNAIKTNCPMTECISIAKELL 248
NSPPLK +MSA+ ++GYAASRSHI NA+KT+CPM+ + IAKE L
Sbjct: 386 NSPPLKKMMSALVKEGYAASRSHIIPNALKTDCPMSHFVRIAKENL 431
>sp|Q9YDY7|TRM1_AERPE N(2),N(2)-dimethylguanosine tRNA methyltransferase
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2-dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase)
Length = 401
Score = 57.0 bits (136), Expect = 2e-07
Identities = 30/104 (28%), Positives = 55/104 (52%)
Frame = -3
Query: 562 LHDAAYLTDMLNLAKQWGWIGCDGKDSLEKLINVMVDESDPKLPFGYIKLDEMASRAKIN 383
L D A+L MLN+A + W+G + +EKL+N + E+ Y+ L +AS+A++N
Sbjct: 296 LGDQAFLEAMLNIAVEAVWLGT--RPRVEKLLNTLHGEAPLSPRSIYVSLTSVASKARVN 353
Query: 382 SPPLKTLMSAIQQKGYAASRSHIKTNAIKTNCPMTECISIAKEL 251
+P ++ ++ GY A +H ++T+ P E + +L
Sbjct: 354 TPKKSKVVELLRSMGYRAVATHFSGEGVRTDAPWGEVLEAVVKL 397
>ref|NP_147490.1| N2,N2-dimethylguanosine tRNA methyltransferase [Aeropyrum pernix]
gi|7450763|pir||H72669 probable N2,N2-dimethylguanosine
tRNA methyltransferase APE0782 - Aeropyrum pernix
(strain K1) gi|5104445|dbj|BAA79760.1| 409aa long
hypothetical N2,N2-dimethylguanosine tRNA
methyltransferase [Aeropyrum pernix]
Length = 409
Score = 57.0 bits (136), Expect = 2e-07
Identities = 30/104 (28%), Positives = 55/104 (52%)
Frame = -3
Query: 562 LHDAAYLTDMLNLAKQWGWIGCDGKDSLEKLINVMVDESDPKLPFGYIKLDEMASRAKIN 383
L D A+L MLN+A + W+G + +EKL+N + E+ Y+ L +AS+A++N
Sbjct: 304 LGDQAFLEAMLNIAVEAVWLGT--RPRVEKLLNTLHGEAPLSPRSIYVSLTSVASKARVN 361
Query: 382 SPPLKTLMSAIQQKGYAASRSHIKTNAIKTNCPMTECISIAKEL 251
+P ++ ++ GY A +H ++T+ P E + +L
Sbjct: 362 TPKKSKVVELLRSMGYRAVATHFSGEGVRTDAPWGEVLEAVVKL 405
>pir||T39993 N2,N2-dimethylguanosine tRNA methyltransferase - fission yeast
(Schizosaccharomyces pombe)
Length = 524
Score = 56.2 bits (134), Expect = 3e-07
Identities = 35/94 (37%), Positives = 49/94 (51%)
Frame = -3
Query: 565 PLHDAAYLTDMLNLAKQWGWIGCDGKDSLEKLINVMVDESDPKLPFGYIKLDEMASRAKI 386
PLHDA +L M +A+ K + ++ + DE P +PF Y L ++ S +
Sbjct: 347 PLHDAEFLKKMRAIAEDLDPEVYGTKRRILGML-ALADEELPDVPF-YFVLSQICSVLRS 404
Query: 385 NSPPLKTLMSAIQQKGYAASRSHIKTNAIKTNCP 284
SPP +SA+ GY S SH K+NAIKTN P
Sbjct: 405 QSPPQNIFVSALLNAGYRVSGSHAKSNAIKTNAP 438
>pir||T46565 tRNA (guanine-N2-)-methyltransferase (EC 2.1.1.32) [imported] -
fission yeast (Schizosaccharomyces pombe)
gi|3393025|emb|CAA11801.1| tRNA
(Guanine-N2-)-Methyltransferase [Schizosaccharomyces
pombe]
Length = 548
Score = 56.2 bits (134), Expect = 3e-07
Identities = 35/94 (37%), Positives = 49/94 (51%)
Frame = -3
Query: 565 PLHDAAYLTDMLNLAKQWGWIGCDGKDSLEKLINVMVDESDPKLPFGYIKLDEMASRAKI 386
PLHDA +L M +A+ K + ++ + DE P +PF Y L ++ S +
Sbjct: 371 PLHDAEFLKKMRAIAEDLDPEVYGTKRRILGML-ALADEELPDVPF-YFVLSQICSVLRS 428
Query: 385 NSPPLKTLMSAIQQKGYAASRSHIKTNAIKTNCP 284
SPP +SA+ GY S SH K+NAIKTN P
Sbjct: 429 QSPPQNIFVSALLNAGYRVSGSHAKSNAIKTNAP 462
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 473,464,538
Number of Sequences: 1393205
Number of extensions: 9584364
Number of successful extensions: 20524
Number of sequences better than 10.0: 52
Number of HSP's better than 10.0 without gapping: 20013
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 20512
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 20669577624
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
