>KMC015769A_C01 KMC015769A_c01
ggaacccgacaattcaaaccaaatcaatctaaagggttcagattcattgggataaagatt
gaagtagagagaaaatgaaaATATTGGGCTCTTTTTAGAAAATGAAAGATTTCCATTCAC
TGAGAGAAATGAAAATATTGAAGTAGCAGCAATGTATACAGCCAACGTAAATATCTATTC
ATTATTATCTAGAGATTGACTACCACCATCATCATCAGAGGGCCTTTCTTCATTCCTGTC
ATCATTGGGAGCCTTCCCTCGCTCATGCCTCTCATTCCACCTTGCCCAAATCCATTTCGA
AATAATGATCCCTCCCAATGCATAAACCTGCCACAGTATGAAAGGATGGGAAGTCTGGGT
AGAGCTCTCAGGGTCAGCTTCAGCCTGCAGAGGTGAAGGAGGAGCAACAGGTGGCGTCTT
CGGGAACCGTACAAGGCTGCGCTTCGAATTCGAATTCGAACTCTCTTCGTTGTGGTGCTG
TTCAGGGGTGGTTGTGGCGGTTTTGTCTCCAAAACTAGGTTTAGGTTT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC015769A_C01 KMC015769A_c01
         (528 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_566963.1| expressed protein; protein id: At3g52230.1, sup...    80  1e-14
pir||S46956 outer membrane protein E24 - spinach gi|515932|emb|C...    72  3e-12
pir||S46542 outer envelope protein omp24, chloroplast - spinach ...    69  3e-11
dbj|BAB85328.1| OJ1656_A11.17 [Oryza sativa (japonica cultivar-g...    56  3e-07
ref|NP_593527.1| hypothetical protein [Schizosaccharomyces pombe...    38  0.084

>ref|NP_566963.1| expressed protein; protein id: At3g52230.1, supported by cDNA:
           33058., supported by cDNA: gi_15450438, supported by
           cDNA: gi_16974440 [Arabidopsis thaliana]
           gi|11357776|pir||T49102 hypothetical protein F4F15.340 -
           Arabidopsis thaliana gi|4678952|emb|CAB41343.1| putative
           protein [Arabidopsis thaliana]
           gi|15450439|gb|AAK96513.1| AT3g52230/F4F15_340
           [Arabidopsis thaliana] gi|16974441|gb|AAL31224.1|
           AT3g52230/F4F15_340 [Arabidopsis thaliana]
           gi|21592837|gb|AAM64787.1| unknown [Arabidopsis
           thaliana]
          Length = 145

 Score = 80.5 bits (197), Expect = 1e-14
 Identities = 49/117 (41%), Positives = 66/117 (55%)
 Frame = -1

Query: 528 KPKPSFGDKTATTTPEQHHNEESSNSNSKRSLVRFPKTPPVAPPSPLQAEADPESSTQTS 349
           KP P+  D  A   P+    +E   S  +   V FP  PP +   P++ EA+P SS +TS
Sbjct: 36  KPPPATIDANA---PKPTGEKEPLKSTYET--VTFPYNPPKSA-EPIKFEAEP-SSGRTS 88

Query: 348 HPFILWQVYALGGIIISKWIWARWNERHERGKAPNDDRNEERPSDDDGGSQSLDNNE 178
           +  ILWQVYALGG ++ KW WARWNER+ER     +   ++   DDD   QS D +E
Sbjct: 89  NSVILWQVYALGGFLVLKWAWARWNERNERSD-KKEATGDDDQKDDDEDDQSSDGHE 144

>pir||S46956 outer membrane protein E24 - spinach gi|515932|emb|CAA56654.1| E24
           ASN [Spinacia oleracea]
          Length = 148

 Score = 72.4 bits (176), Expect = 3e-12
 Identities = 35/85 (41%), Positives = 49/85 (57%), Gaps = 1/85 (1%)
 Frame = -1

Query: 468 EESSNSNSKR-SLVRFPKTPPVAPPSPLQAEADPESSTQTSHPFILWQVYALGGIIISKW 292
           EE S SN+K   +VRFP T P   P  L+ E     + + ++P ILWQ+YALGG I+ KW
Sbjct: 59  EEGSGSNTKTPDVVRFPATQPEVAPLKLENE----DAQEDTNPLILWQIYALGGFIVLKW 114

Query: 291 IWARWNERHERGKAPNDDRNEERPS 217
            W +W ER     +  +D+ +  PS
Sbjct: 115 AWGKWQERKANNGSSGEDQPQPPPS 139

>pir||S46542 outer envelope protein omp24, chloroplast - spinach
           gi|414200|emb|CAA53243.1| chloroplast outer envelope 24
           kD protein (omp24) [Spinacia oleracea]
          Length = 148

 Score = 69.3 bits (168), Expect = 3e-11
 Identities = 34/85 (40%), Positives = 48/85 (56%), Gaps = 1/85 (1%)
 Frame = -1

Query: 468 EESSNSNSKR-SLVRFPKTPPVAPPSPLQAEADPESSTQTSHPFILWQVYALGGIIISKW 292
           EE S S +K   +VRFP T P   P  L+ E     + + ++P ILWQ+YALGG I+ KW
Sbjct: 59  EEGSGSYTKTPDVVRFPATQPEVAPLKLENE----DAQEDTNPLILWQIYALGGFIVLKW 114

Query: 291 IWARWNERHERGKAPNDDRNEERPS 217
            W +W ER     +  +D+ +  PS
Sbjct: 115 AWGKWQERKANNGSSGEDQPQPPPS 139

>dbj|BAB85328.1| OJ1656_A11.17 [Oryza sativa (japonica cultivar-group)]
          Length = 106

 Score = 55.8 bits (133), Expect = 3e-07
 Identities = 31/95 (32%), Positives = 48/95 (49%), Gaps = 10/95 (10%)
 Frame = -1

Query: 498 ATTTPEQHHNEESSNSNSKR---SLVRFPKT---PPVAPPSPLQAEAD----PESSTQTS 349
           A ++PEQ       ++ ++R   S VRF  +   P    P P++ E +    P  + +  
Sbjct: 4   AGSSPEQAAGNNKEDAEARRQPPSTVRFYPSADQPKARQPPPIKLEEEDVPPPPVADEEM 63

Query: 348 HPFILWQVYALGGIIISKWIWARWNERHERGKAPN 244
            P  LWQVYALG  I+ +W W +W E  ER  +P+
Sbjct: 64  APRNLWQVYALGAFIVLRWAWVKWKESKERDDSPD 98

>ref|NP_593527.1| hypothetical protein [Schizosaccharomyces pombe]
           gi|12188977|emb|CAC21486.1| hypothetical protein;
           similar to proteoglycans; similar to S. cerevisiae MUC1
           [Schizosaccharomyces pombe]
          Length = 529

 Score = 37.7 bits (86), Expect = 0.084
 Identities = 27/101 (26%), Positives = 43/101 (41%), Gaps = 15/101 (14%)
 Frame = -1

Query: 522 KPSFGDKTATTTPEQHHNEESSNSNSKRSLVRFPKTPPVAPPS---PLQAEADPESSTQT 352
           KP+  D T+++TP   + E  +N   K S  R     P   PS    + A    ESST +
Sbjct: 424 KPALPDATSSSTPTTENKESWTNQGIKSSQQRSANASPATSPSNQASIHASFTKESSTHS 483

Query: 351 SHPFILWQVYALGGIII------------SKWIWARWNERH 265
           S  F L  +++    ++            S+ + ARW E +
Sbjct: 484 SPSFTLESLFSGAPRVVELTRFSQPSPACSRALLARWKEEY 524

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 483,110,832
Number of Sequences: 1393205
Number of extensions: 10951055
Number of successful extensions: 53625
Number of sequences better than 10.0: 64
Number of HSP's better than 10.0 without gapping: 44871
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 52389
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 17308240320
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MFB091f12_f BP040667 1 478 2 MWM151d10_f AV767088 97 528 3 MF022a05_f BP029402 97 586

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