>KMC015704A_C01 KMC015704A_c01
CTCTAAAAACGTTTTATTTTTATCATAAAAGGGAACGGGCAGAAACGTTCTCTTTTTAAC
ACAATATTTCTTCTATTAGACGCTGGTATTAGAAGATTTACCAACAGTCATGATCAACCA
ATAAATGAACCACATGATATTGATGACATTTTTAAACCAAAGTTCAATCCTCAAACGGAC
AGACTAACAAGAAAGGGGTGAGATCGAAAGTGTAAATCATCAAGCAGCAACTCCGTCCTG
GAGCAGCATCTGATTGAAGTGCCAGGTACCATGTCCTTGGCCAACTCTTCTAAGCTGAGC
AACGTACTCCGAGATTGGTTTAATAGATTCCACCTGTTCACCCAAATACTCGCTTTCAAT
AAAATCTGTCAACTGCGCATCGTTGTTCTTTGAGGCAACACTGTGCAAGTTAAGGAGCTT
TTCATTGGTTAGCTTCTCCAATGACAGAGCAAGTTCCATTGCATATAGCGCATCTCCCTT
TTCCTCATGTTCAAACTCAGAAGGAGGCATCACTATAGACTGCAACTTCACTCTCCCACC
CCGTTTGTTCTGATACTCCATCAATTTTTCAGCATGCTCCCTTTCCTCCATACTTGACTC
CTTGAAAAACTTGGCAAGACCCTTAAGCGCAACATTGTCCCTGTCGAAGTAGGCAAACAT
TGCATGGTAAACATACGAAACAGTGTACTCCACATTGATCTGCTCGTTAATCGCAGCCTC
ACACTCATCTACGAACTTCTGGCGAGCAAGGGAAGCTTGAGGAACAGTGGGAACAAGGTC
GAGCTCCTTCTTGACCTCTTCAAAGGGTTCAAAAACGACGCCGGTTAGGGGGCGGTTGTT
GGAGCCCTTGGTGGCGCAGGGGAGAAAGGTTTTGGGTGAAACGGAAGAGGAAAACAGAGG
GGGGAGAAGATGGTCGCCGCCATTGGAGTTGAGAAGAGAGAAAGAGGAAGCTGCTCTGAG
GAGcatggttgttgttgagcaaaaacagggaacgaatgaatgaagaaagagggttcagtt
cggagtgtatgtgcgtgtgttgc

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC015704A_C01 KMC015704A_c01
         (1043 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

dbj|BAB64536.1| ferritin [Glycine max]                                405  e-112
pir||T08124 ferritin 2 precursor - cowpea gi|2970654|gb|AAC06027...   365  e-100
gb|AAN06322.1| ferritin 2 [Nicotiana tabacum]                         362  4e-99
gb|AAM11429.1| ferritin [Nicotiana tabacum]                           362  7e-99
dbj|BAB64537.1| ferritin [Glycine max]                                356  3e-97

>dbj|BAB64536.1| ferritin [Glycine max]
          Length = 256

 Score =  405 bits (1041), Expect = e-112
 Identities = 212/257 (82%), Positives = 226/257 (87%), Gaps = 9/257 (3%)
 Frame = -3

Query: 966 MLLRAASSFSLLNSNGGDHLLPPLFSSSVS--------PKTFLPCATKGSNNRPLTGVVF 811
           MLLR ASSFSLL +N  DH+LP   SSS           K  +PCATK +NNRPLTGVVF
Sbjct: 1   MLLRTASSFSLLKANA-DHILPLPNSSSSGIIRYSQSLGKNLVPCATKDTNNRPLTGVVF 59

Query: 810 EPFEEVKKELDLVPTVPQASLARQKFVDECEAAINEQINVEYTVSYVYHAMFAYFDRDNV 631
           EPFEEVKKELDLVPTVPQASLARQK+ D+CEA INEQINVEY VSYVYHAMFAYFDRDNV
Sbjct: 60  EPFEEVKKELDLVPTVPQASLARQKYTDDCEATINEQINVEYNVSYVYHAMFAYFDRDNV 119

Query: 630 ALKGLAKFFKESSMEEREHAEKLMEYQNKRGGRVKLQSIVMPPSEFEHEEKGDALYAMEL 451
           ALKGLAKFFKESS EEREHAEKLMEYQNKRGG+VKLQSIVMP SEF+HEEKGDALYAMEL
Sbjct: 120 ALKGLAKFFKESSEEEREHAEKLMEYQNKRGGKVKLQSIVMPLSEFDHEEKGDALYAMEL 179

Query: 450 ALSLEKLTNEKLLNLHSVASKNNDAQLTDFIESEYLGEQVESIKPISEYVAQLRRVGQGH 271
           ALSLEKLTNEKLLNLHSVASKNND QL DFIESE+LGEQVE+IK ISEYVAQLRRVG+GH
Sbjct: 180 ALSLEKLTNEKLLNLHSVASKNNDVQLADFIESEFLGEQVEAIKKISEYVAQLRRVGKGH 239

Query: 270 GTWHFNQMLL-QDGVAA 223
           G WHF+QMLL ++GVAA
Sbjct: 240 GVWHFDQMLLHEEGVAA 256

>pir||T08124 ferritin 2 precursor - cowpea gi|2970654|gb|AAC06027.1| ferritin
           subunit cowpea2 precursor [Vigna unguiculata]
          Length = 250

 Score =  365 bits (937), Expect = e-100
 Identities = 191/248 (77%), Positives = 214/248 (86%), Gaps = 3/248 (1%)
 Frame = -3

Query: 966 MLLR--AASSFSLLNSNGGDHLLPPLFSSSVSPKTFLPCATKGS-NNRPLTGVVFEPFEE 796
           MLLR  AASS SL N N       P+ +++ S    +  A KGS N+R LTGV+FEPFEE
Sbjct: 1   MLLRTAAASSLSLFNPNAEPSRSVPVLANNAS--RLVVRAAKGSTNHRALTGVIFEPFEE 58

Query: 795 VKKELDLVPTVPQASLARQKFVDECEAAINEQINVEYTVSYVYHAMFAYFDRDNVALKGL 616
           VKKELDLVPTVPQASLARQK+VDE EAA+NEQINVEY VSYVYHA+FAYFDRDNVAL+GL
Sbjct: 59  VKKELDLVPTVPQASLARQKYVDESEAAVNEQINVEYNVSYVYHALFAYFDRDNVALRGL 118

Query: 615 AKFFKESSMEEREHAEKLMEYQNKRGGRVKLQSIVMPPSEFEHEEKGDALYAMELALSLE 436
           AKFFKESS EEREHAEKLMEYQN+RGG+VKLQSIVMP SEF+H +KGDAL+AMELALSLE
Sbjct: 119 AKFFKESSEEEREHAEKLMEYQNRRGGKVKLQSIVMPLSEFDHADKGDALHAMELALSLE 178

Query: 435 KLTNEKLLNLHSVASKNNDAQLTDFIESEYLGEQVESIKPISEYVAQLRRVGQGHGTWHF 256
           KLTNEKLL+LHSVA+KN D QL DF+ESE+LGEQVESIK ISEYVAQLRRVG+GHG WHF
Sbjct: 179 KLTNEKLLHLHSVATKNGDVQLADFVESEFLGEQVESIKRISEYVAQLRRVGKGHGVWHF 238

Query: 255 NQMLLQDG 232
           +QMLL +G
Sbjct: 239 DQMLLHEG 246

>gb|AAN06322.1| ferritin 2 [Nicotiana tabacum]
          Length = 259

 Score =  362 bits (930), Expect = 4e-99
 Identities = 187/255 (73%), Positives = 217/255 (84%), Gaps = 11/255 (4%)
 Frame = -3

Query: 966 MLLRAASSFSLLNSNGGDHLLPPLFSSS-------VSPKT----FLPCATKGSNNRPLTG 820
           MLL+ A +F+LLNS+G ++L P L +SS        S K+     + CA+KGSN +PLTG
Sbjct: 1   MLLKLAPAFTLLNSHG-ENLSPMLSTSSQGFVLKNFSTKSRNGLLVVCASKGSNTKPLTG 59

Query: 819 VVFEPFEEVKKELDLVPTVPQASLARQKFVDECEAAINEQINVEYTVSYVYHAMFAYFDR 640
           VVFEPFEEVKKEL LVPTVPQ SLAR K+ D+CEAA+NEQINVEY VSYVYH M+AYFDR
Sbjct: 60  VVFEPFEEVKKELMLVPTVPQVSLARHKYSDQCEAAVNEQINVEYNVSYVYHGMYAYFDR 119

Query: 639 DNVALKGLAKFFKESSMEEREHAEKLMEYQNKRGGRVKLQSIVMPPSEFEHEEKGDALYA 460
           DNVALKGLA+FFKESS EER HAEKLMEYQNKRGG+VKLQSI+MP SEF+H E+GDALYA
Sbjct: 120 DNVALKGLARFFKESSEEERGHAEKLMEYQNKRGGKVKLQSILMPLSEFDHAEEGDALYA 179

Query: 459 MELALSLEKLTNEKLLNLHSVASKNNDAQLTDFIESEYLGEQVESIKPISEYVAQLRRVG 280
           MELALSL KLTN+KLLNLH+VA++NND QL DF+ES+YL EQVE+IK ISEYVAQLRRVG
Sbjct: 180 MELALSLAKLTNQKLLNLHAVATRNNDVQLADFVESKYLREQVEAIKMISEYVAQLRRVG 239

Query: 279 QGHGTWHFNQMLLQD 235
           +GHG WHF+QMLLQ+
Sbjct: 240 KGHGVWHFDQMLLQE 254

>gb|AAM11429.1| ferritin [Nicotiana tabacum]
          Length = 251

 Score =  362 bits (928), Expect = 7e-99
 Identities = 187/253 (73%), Positives = 215/253 (84%), Gaps = 5/253 (1%)
 Frame = -3

Query: 966 MLLRAASSFSLLNSNGGDHLLPPLFSSS--VSPKT---FLPCATKGSNNRPLTGVVFEPF 802
           MLL+AA +F+LLN+ G +  L PLFSSS   SPK    F+  A+K +N++PLTGVVFEPF
Sbjct: 1   MLLKAAPAFALLNTQGEN--LSPLFSSSKSFSPKNGNRFVVSASKATNHKPLTGVVFEPF 58

Query: 801 EEVKKELDLVPTVPQASLARQKFVDECEAAINEQINVEYTVSYVYHAMFAYFDRDNVALK 622
           EE+KKEL LVP VP  SL RQK+ D+CEAAINEQINVEY  SYVYHAMFAYFDRDNVALK
Sbjct: 59  EELKKELMLVPAVPDTSLCRQKYSDDCEAAINEQINVEYNNSYVYHAMFAYFDRDNVALK 118

Query: 621 GLAKFFKESSMEEREHAEKLMEYQNKRGGRVKLQSIVMPPSEFEHEEKGDALYAMELALS 442
           GLAKFFKESS+EEREHAEKLME+QNKRGGRVKL SI  PP+EF+H EKGDALYAMELAL 
Sbjct: 119 GLAKFFKESSLEEREHAEKLMEFQNKRGGRVKLLSICAPPTEFDHCEKGDALYAMELALC 178

Query: 441 LEKLTNEKLLNLHSVASKNNDAQLTDFIESEYLGEQVESIKPISEYVAQLRRVGQGHGTW 262
           LEKLTN++LLNLH+VAS++ND  L DF+ESE+L EQV++IK ISEYVAQLRRVGQGHG W
Sbjct: 179 LEKLTNQRLLNLHAVASRSNDVHLADFLESEFLVEQVDAIKKISEYVAQLRRVGQGHGVW 238

Query: 261 HFNQMLLQDGVAA 223
            F+QMLL +G AA
Sbjct: 239 QFDQMLLNEGAAA 251

>dbj|BAB64537.1| ferritin [Glycine max]
          Length = 247

 Score =  356 bits (914), Expect = 3e-97
 Identities = 189/250 (75%), Positives = 211/250 (83%), Gaps = 5/250 (2%)
 Frame = -3

Query: 966 MLLR----AASSFSLLNSNGGDHLLPPLFSSSVSPKTFLPCATKGS-NNRPLTGVVFEPF 802
           MLLR    +ASS SL + N      PP    SV  +  +  A KGS N+R LTGV+FEPF
Sbjct: 1   MLLRTAAASASSLSLFSPNAE----PP---RSVPARGLVVRAAKGSTNHRALTGVIFEPF 53

Query: 801 EEVKKELDLVPTVPQASLARQKFVDECEAAINEQINVEYTVSYVYHAMFAYFDRDNVALK 622
           EEVKKELDLVPTVPQASLARQK+VDE E+A+NEQINVEY VSYVYHAMFAYFDRDNVAL+
Sbjct: 54  EEVKKELDLVPTVPQASLARQKYVDESESAVNEQINVEYNVSYVYHAMFAYFDRDNVALR 113

Query: 621 GLAKFFKESSMEEREHAEKLMEYQNKRGGRVKLQSIVMPPSEFEHEEKGDALYAMELALS 442
           GLAKFFKESS EEREHAEKLMEYQNKRGG+VKLQSIVMP S+F+H +KGDAL+AMELALS
Sbjct: 114 GLAKFFKESSEEEREHAEKLMEYQNKRGGKVKLQSIVMPLSDFDHADKGDALHAMELALS 173

Query: 441 LEKLTNEKLLNLHSVASKNNDAQLTDFIESEYLGEQVESIKPISEYVAQLRRVGQGHGTW 262
           LEKLTNEKLLNLHSVA+KN D QL DF+E+EYLGEQVE+IK ISEYVAQLR+  QGHG W
Sbjct: 174 LEKLTNEKLLNLHSVATKNGDVQLADFVETEYLGEQVEAIKRISEYVAQLRKSWQGHGVW 233

Query: 261 HFNQMLLQDG 232
           HF+QMLL +G
Sbjct: 234 HFDQMLLHEG 243

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 910,687,110
Number of Sequences: 1393205
Number of extensions: 21338578
Number of successful extensions: 83585
Number of sequences better than 10.0: 401
Number of HSP's better than 10.0 without gapping: 70932
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 81559
length of database: 448,689,247
effective HSP length: 124
effective length of database: 275,931,827
effective search space used: 61532797421
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 SPD087e09_f BP050957 1 484 2 SPD087e07_f BP050955 1 499 3 MFB016c09_f BP035096 1 480 4 MWM141h05_f AV766937 1 383 5 SPD058c06_f BP048602 1 521 6 MFBL005g07_f BP041544 58 594 7 MFB037f01_f BP036718 476 1043

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