>KMC015148A_C01 KMC015148A_c01
tacgggcccccctccaggtttttttTTTTTTTTTTTAGATAACAGAAACTTTTATTGGAT
TAGAAACATAACAGATACAAAGAGAAATAGAACATGGGAGTAAAAGAGTTCAATCCCCCA
AGTTACAAGGAACAAAAAGGTGACAAAAGGCAACATAACAACAACCCACTAAAACAACCC
AACAAGACGAAAGCAGGACCTGAGCAGTACCACAGTCCACACCGCTGCAGTTGGAACAAG
CAGATGGATACAACAAAAAACAAAATACGATCACAACAACCCACCCACATCAGCACCAGA
AGCGGATTCAGTCGAAGGATCATATAATTATTGAAAGCTTAACTTGAATGATGTACTTCA
TTGCATAATTTGTAGAACAATTAGTCATCCAAAGTCTAATATCTTTTGAGAAAGTTCGTG
AACCATTTTGCGGATAGCTTTTTGTACCTCTTCAATCCATTTTCGTAATCCACAAAATTC
ATTCCAAATCGCACTGTATAACCTGAAGACCATTCATAATTGTCCAACAATGACCATGCG
AAATATCCTTTGACGTTTGATCCATTCCTTATTGCAGATTGAAGATAAAAGAGATGTCGA
AAATAATAGTCAATTCTGAAAGTGTCT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC015148A_C01 KMC015148A_c01
         (627 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAL07491.1|AF414608_1 prunasin hydrolase isoform PH I precurs...   126  3e-28
gb|AAL35324.1|AF411131_1 prunasin hydrolase isoform PH C precurs...   126  3e-28
gb|AAL07434.1|AF413213_1 prunasin hydrolase isoform PH C precurs...   126  3e-28
pir||T09657 beta-glucosidase (EC 3.2.1.21) precursor, cyanogenic...   126  3e-28
gb|AAF34650.1|AF221526_1 prunasin hydrolase isoform PHA precurso...   125  3e-28

>gb|AAL07491.1|AF414608_1 prunasin hydrolase isoform PH I precursor [Prunus serotina]
          Length = 513

 Score =  126 bits (316), Expect = 3e-28
 Identities = 53/75 (70%), Positives = 66/75 (87%)
 Frame = -2

Query: 626 DTFRIDYYFRHLFYLQSAIRNGSNVKGYFAWSLLDNYEWSSGYTVRFGMNFVDYENGLKR 447
           D  RIDYY+RHL YLQ+AI+ G+NV+GYFAWSLLDN+EWS GYTVRFG+N++DY+NGL+R
Sbjct: 414 DANRIDYYYRHLCYLQAAIKEGANVQGYFAWSLLDNFEWSEGYTVRFGINYIDYDNGLER 473

Query: 446 YKKLSAKWFTNFLKR 402
           + KLS  WF +FLKR
Sbjct: 474 HSKLSTHWFKSFLKR 488

>gb|AAL35324.1|AF411131_1 prunasin hydrolase isoform PH C precursor [Prunus serotina]
          Length = 542

 Score =  126 bits (316), Expect = 3e-28
 Identities = 53/75 (70%), Positives = 66/75 (87%)
 Frame = -2

Query: 626 DTFRIDYYFRHLFYLQSAIRNGSNVKGYFAWSLLDNYEWSSGYTVRFGMNFVDYENGLKR 447
           D  RIDYY+RHL YLQ+AI+ G+NV+GYFAWSLLDN+EWS GYTVRFG+N++DY+NGL+R
Sbjct: 443 DVNRIDYYYRHLCYLQAAIKEGANVQGYFAWSLLDNFEWSEGYTVRFGINYIDYDNGLER 502

Query: 446 YKKLSAKWFTNFLKR 402
           + KLS  WF +FLKR
Sbjct: 503 HSKLSTHWFKSFLKR 517

>gb|AAL07434.1|AF413213_1 prunasin hydrolase isoform PH C precursor [Prunus serotina]
          Length = 517

 Score =  126 bits (316), Expect = 3e-28
 Identities = 53/75 (70%), Positives = 66/75 (87%)
 Frame = -2

Query: 626 DTFRIDYYFRHLFYLQSAIRNGSNVKGYFAWSLLDNYEWSSGYTVRFGMNFVDYENGLKR 447
           D  RIDYY+RHL YLQ+AI+ G+NV+GYFAWSLLDN+EWS GYTVRFG+N++DY+NGL+R
Sbjct: 418 DVNRIDYYYRHLCYLQAAIKEGANVQGYFAWSLLDNFEWSEGYTVRFGINYIDYDNGLER 477

Query: 446 YKKLSAKWFTNFLKR 402
           + KLS  WF +FLKR
Sbjct: 478 HSKLSTHWFKSFLKR 492

>pir||T09657 beta-glucosidase (EC 3.2.1.21) precursor, cyanogenic - black cherry
           gi|1236961|gb|AAA93032.1| prunasin hydrolase isoform PH
           I precursor [Prunus serotina]
          Length = 549

 Score =  126 bits (316), Expect = 3e-28
 Identities = 53/75 (70%), Positives = 66/75 (87%)
 Frame = -2

Query: 626 DTFRIDYYFRHLFYLQSAIRNGSNVKGYFAWSLLDNYEWSSGYTVRFGMNFVDYENGLKR 447
           D  RIDYY+RHL YLQ+AI+ G+NV+GYFAWSLLDN+EWS GYTVRFG+N++DY+NGL+R
Sbjct: 450 DANRIDYYYRHLCYLQAAIKEGANVQGYFAWSLLDNFEWSEGYTVRFGINYIDYDNGLER 509

Query: 446 YKKLSAKWFTNFLKR 402
           + KLS  WF +FLKR
Sbjct: 510 HSKLSTHWFKSFLKR 524

>gb|AAF34650.1|AF221526_1 prunasin hydrolase isoform PHA precursor [Prunus serotina]
          Length = 537

 Score =  125 bits (315), Expect = 3e-28
 Identities = 54/76 (71%), Positives = 64/76 (84%)
 Frame = -2

Query: 626 DTFRIDYYFRHLFYLQSAIRNGSNVKGYFAWSLLDNYEWSSGYTVRFGMNFVDYENGLKR 447
           DT RIDYY+ HL YLQ+AI++G  VKGYFAWS+LDN+EW+SGYTVRFG+N+VDY+NGLKR
Sbjct: 438 DTMRIDYYYHHLCYLQAAIKDGVRVKGYFAWSVLDNFEWNSGYTVRFGINYVDYDNGLKR 497

Query: 446 YKKLSAKWFTNFLKRY 399
             K SA W  NFLK Y
Sbjct: 498 RSKFSAHWLKNFLKNY 513

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 567,540,101
Number of Sequences: 1393205
Number of extensions: 12433738
Number of successful extensions: 37799
Number of sequences better than 10.0: 501
Number of HSP's better than 10.0 without gapping: 35777
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 37613
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 25586195130
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MFB091a11_f BP040621 1 484 2 MFB017d10_f BP035181 23 632 3 MF073f04_f BP032182 24 486 4 MF018h03_f BP029220 26 182 5 SPD005d02_f BP044389 29 632 6 MF024c09_f BP029529 37 212 7 MFB002d08_f BP034038 37 596 8 MFB076g02_f BP039573 37 547 9 MF056c12_f BP031237 37 554 10 MF090a03_f BP033001 37 553 11 MF060c09_f BP031457 37 549 12 SPD091d05_f BP051266 37 607 13 MF096g08_f BP033317 37 610 14 MF073g03_f BP032188 37 543 15 MF066b01_f BP031793 37 492 16 MF076h04_f BP032345 37 561 17 MF092c06_f BP033114 37 525 18 MF016h12_f BP029119 37 521 19 MF019f03_f BP029267 37 506 20 MWM048g01_f AV765445 37 570 21 MF089c05_f BP032965 37 477 22 MF025h12_f BP029608 37 357 23 SPDL084e06_f BP057262 37 563 24 MF032b10_f BP029957 37 407 25 MF026a12_f BP029618 37 448 26 MFB017b05_f BP035158 37 498 27 MF079h12_f BP032500 37 345 28 SPD073b12_f BP049817 37 364 29 MFB077e04_f BP039629 37 501 30 MF070f12_f BP032024 38 489 31 MF011e02_f BP028812 40 488 32 MF032f04_f BP029984 40 563 33 MF025d04_f BP029578 40 546 34 MFB071g08_f BP039189 40 578 35 MF043g04_f BP030575 40 563 36 MF015d06_f BP029032 40 224 37 SPDL063b07_f BP055895 40 535 38 MF005c10_f BP028487 41 563 39 MF085c02_f BP032763 48 523 40 SPD079f07_f BP050336 52 517 41 MF072h10_f BP032146 56 574 42 MF070c08_f BP032002 57 369 43 MF034f05_f BP030104 61 464 44 MF092f06_f BP033129 61 498 45 SPDL016h11_f BP053027 61 576

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