>KMC015139A_C01 KMC015139A_c01
agatgcaaataatgcttcgtaatacatgcagcaaaAAAAAAAAAAAATTATATCACGGCT
GGATATTGAAAGTCATCGTCAAACATTAGTTCACAGTAAGGAAGATATAAATAAAAAAGC
ACTAACATCCACTGCTTACAATGCAGCCAAAAAAGATGTTAGGGAAAGTGAATTTGAGAG
ACTCAAATAAAATAAACTTTAACAAAGACTCTGACCCACCTACCTCTCAAGAAGCATAAC
CAGTTCACAGTGTGGAGTGTGTGGAAAAAGATCAACGGCCATAGCTTTTACTGGTTGGAA
GGCCTCTGAGATGGGCATGGACTTGGCTCGATGCCGTGCTAGACCTGCCATGCTCCTGTT
TCTCCATCCTCTATTGTCTTTTTTTACTTTCTCATTAGTTGCAGGGGAAGGCGTGCAAAG
CTCAATAGCATTTGCCACCAAAGTTTCAGGATTACAGGATATGTAAACAAGCCTCCGCAG
GCCTGGATGAGTCCTCAAAGCTTTAATCACAGTTGGATGCAGTCCAGCACGTGGTGGATC
AACAATAGCAACAACATTTTTAAACTGTTTCCT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC015139A_C01 KMC015139A_c01
         (573 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAM91097.1| At2g28450/T1B3.3 [Arabidopsis thaliana] gi|250904...   182  3e-45
ref|NP_180412.1| putative RNA methyltransferase; protein id: At2...   182  3e-45
emb|CAD39349.1| OSJNBa0094O15.17 [Oryza sativa (japonica cultiva...   177  8e-44
gb|AAH17184.1| Similar to HpaII tiny  fragments locus 9C [Homo s...    93  2e-18
dbj|BAB15067.1| unnamed protein product [Homo sapiens] gi|154264...    93  2e-18

>gb|AAM91097.1| At2g28450/T1B3.3 [Arabidopsis thaliana] gi|25090447|gb|AAN72304.1|
            At2g28450/T1B3.3 [Arabidopsis thaliana]
          Length = 809

 Score =  182 bits (461), Expect = 3e-45
 Identities = 90/117 (76%), Positives = 100/117 (84%)
 Frame = -1

Query: 573  RKQFKNVVAIVDPPRAGLHPTVIKALRTHPGLRRLVYISCNPETLVANAIELCTPSPATN 394
            + QFKNVVAIVDPPR+GLHP VIKALRTHP L+RLVYISCNPETLVANAIELCTPS    
Sbjct: 693  KPQFKNVVAIVDPPRSGLHPAVIKALRTHPRLKRLVYISCNPETLVANAIELCTPSFDEP 752

Query: 393  EKVKKDNRGWRNRSMAGLARHRAKSMPISEAFQPVKAMAVDLFPHTPHCELVMLLER 223
            ++  K+ RG +   +A LARHRAKSMP SEAF+PVKAMAVDLFPHT HCE+VMLLER
Sbjct: 753  DRGNKNYRGRKKIGIAALARHRAKSMPTSEAFRPVKAMAVDLFPHTDHCEMVMLLER 809

>ref|NP_180412.1| putative RNA methyltransferase; protein id: At2g28450.1 [Arabidopsis
            thaliana] gi|25407988|pir||A84685 probable RNA
            methyltransferase [imported] - Arabidopsis thaliana
            gi|4432832|gb|AAD20681.1| putative RNA methyltransferase
            [Arabidopsis thaliana]
          Length = 850

 Score =  182 bits (461), Expect = 3e-45
 Identities = 90/117 (76%), Positives = 100/117 (84%)
 Frame = -1

Query: 573  RKQFKNVVAIVDPPRAGLHPTVIKALRTHPGLRRLVYISCNPETLVANAIELCTPSPATN 394
            + QFKNVVAIVDPPR+GLHP VIKALRTHP L+RLVYISCNPETLVANAIELCTPS    
Sbjct: 734  KPQFKNVVAIVDPPRSGLHPAVIKALRTHPRLKRLVYISCNPETLVANAIELCTPSFDEP 793

Query: 393  EKVKKDNRGWRNRSMAGLARHRAKSMPISEAFQPVKAMAVDLFPHTPHCELVMLLER 223
            ++  K+ RG +   +A LARHRAKSMP SEAF+PVKAMAVDLFPHT HCE+VMLLER
Sbjct: 794  DRGNKNYRGRKKIGIAALARHRAKSMPTSEAFRPVKAMAVDLFPHTDHCEMVMLLER 850

>emb|CAD39349.1| OSJNBa0094O15.17 [Oryza sativa (japonica cultivar-group)]
          Length = 886

 Score =  177 bits (449), Expect = 8e-44
 Identities = 87/115 (75%), Positives = 95/115 (81%)
 Frame = -1

Query: 567  QFKNVVAIVDPPRAGLHPTVIKALRTHPGLRRLVYISCNPETLVANAIELCTPSPATNEK 388
            QF+++VAIVDPPR GLHPTVIKALRTHP +RRLVYISCNP++LVANAIELCTPS    EK
Sbjct: 752  QFEDIVAIVDPPRVGLHPTVIKALRTHPRIRRLVYISCNPDSLVANAIELCTPSSEKQEK 811

Query: 387  VKKDNRGWRNRSMAGLARHRAKSMPISEAFQPVKAMAVDLFPHTPHCELVMLLER 223
              K NRGWR  S AGLAR R KSMP SE F P +AMAVDLFPHT HCE+VML ER
Sbjct: 812  -NKGNRGWRTMSSAGLARQRTKSMPNSEPFVPKRAMAVDLFPHTSHCEMVMLFER 865

>gb|AAH17184.1| Similar to HpaII tiny  fragments locus 9C [Homo sapiens]
          Length = 613

 Score = 93.2 bits (230), Expect = 2e-18
 Identities = 51/114 (44%), Positives = 68/114 (58%), Gaps = 1/114 (0%)
 Frame = -1

Query: 561 KNVVAIVDPPRAGLHPTVIKALRTHPGLRRLVYISCNPETLVANAIELC-TPSPATNEKV 385
           +++VAI+DPPRAGLH  VI A+R    LRRL+Y+SCNP   + N ++LC  PS       
Sbjct: 491 QHLVAILDPPRAGLHSKVILAIRRAKNLRRLLYVSCNPRAAMGNFVDLCRAPS------- 543

Query: 384 KKDNRGWRNRSMAGLARHRAKSMPISEAFQPVKAMAVDLFPHTPHCELVMLLER 223
                            +R K +P    F+PVKA+AVDLFP TPHCE+++L ER
Sbjct: 544 -----------------NRVKGIP----FRPVKAVAVDLFPQTPHCEMLILFER 576

>dbj|BAB15067.1| unnamed protein product [Homo sapiens]
           gi|15426494|gb|AAH13352.1|AAH13352 HpaII tiny  fragments
           locus 9C [Homo sapiens]
          Length = 475

 Score = 93.2 bits (230), Expect = 2e-18
 Identities = 51/114 (44%), Positives = 68/114 (58%), Gaps = 1/114 (0%)
 Frame = -1

Query: 561 KNVVAIVDPPRAGLHPTVIKALRTHPGLRRLVYISCNPETLVANAIELC-TPSPATNEKV 385
           +++VAI+DPPRAGLH  VI A+R    LRRL+Y+SCNP   + N ++LC  PS       
Sbjct: 353 QHLVAILDPPRAGLHSKVILAIRRAKNLRRLLYVSCNPRAAMGNFVDLCRAPS------- 405

Query: 384 KKDNRGWRNRSMAGLARHRAKSMPISEAFQPVKAMAVDLFPHTPHCELVMLLER 223
                            +R K +P    F+PVKA+AVDLFP TPHCE+++L ER
Sbjct: 406 -----------------NRVKGIP----FRPVKAVAVDLFPQTPHCEMLILFER 438

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 498,798,148
Number of Sequences: 1393205
Number of extensions: 10778925
Number of successful extensions: 29703
Number of sequences better than 10.0: 202
Number of HSP's better than 10.0 without gapping: 28415
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 29562
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 21243732558
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MFBL019b12_f BP042202 1 443 2 MFB008f12_f BP034503 31 573 3 MWM047f01_f AV765419 32 421

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