Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC014847A_C01 KMC014847A_c01
(523 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAC63273.1| SKP1-like protein [Nicotiana clevelandii] 80 2e-14
pir||T17030 fimbriata-associated protein - garden snapdragon (fr... 78 7e-14
ref|NP_565296.1| E3 ubiquitin ligase SCF complex subunit SKP1/AS... 78 7e-14
gb|AAD34458.1| Skp1 [Medicago sativa] 77 2e-13
dbj|BAB85607.1| kinetochore protein [Brassica juncea] 73 2e-12
>gb|AAC63273.1| SKP1-like protein [Nicotiana clevelandii]
Length = 153
Score = 79.7 bits (195), Expect = 2e-14
Identities = 52/136 (38%), Positives = 70/136 (51%), Gaps = 23/136 (16%)
Frame = -2
Query: 501 SLSKEMKAVEIFIDDDDKAFIIPLPEVSSQDLVKIIEYCKQRRTAA----------NAKD 352
S++ E + ++ I+DD IPLP V+S+ L K+IEYCK+ AA + K
Sbjct: 19 SVALESQTIKHMIEDDCADTSIPLPNVTSKILAKVIEYCKRHVDAASKTEDKAVEDDLKA 78
Query: 351 FDAEFAKALNNKELLSLIAAANYLNMADLLDLLSQCIVDRV*NTI-------------FT 211
FDA+F K ++ L LI AANYLN+ LLDL Q + D + FT
Sbjct: 79 FDADFVK-VDQSTLFDLILAANYLNIKSLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFT 137
Query: 210 KEEEASLREEMPWAFE 163
EEE +R E WAFE
Sbjct: 138 PEEEEEVRRENAWAFE 153
>pir||T17030 fimbriata-associated protein - garden snapdragon (fragment)
gi|2673868|emb|CAA75117.1| fimbriata-associated protein
[Antirrhinum majus]
Length = 161
Score = 77.8 bits (190), Expect = 7e-14
Identities = 53/142 (37%), Positives = 70/142 (48%), Gaps = 29/142 (20%)
Frame = -2
Query: 501 SLSKEMKAVEIFIDDDDKAFIIPLPEVSSQDLVKIIEYCKQRRTAANA------------ 358
SL+ E + ++ I+DD +IPLP V+ + L K+IEYCK+ AA A
Sbjct: 21 SLALESQTIKHMIEDDCADNVIPLPNVTGKILSKVIEYCKRHVDAAAAKADDKLASTGTS 80
Query: 357 ----KDFDAEFAKALNNKELLSLIAAANYLNMADLLDLLSQCIVDRV*NTI--------- 217
K FDA+F K ++ L LI AANYLN+ LLDL Q + D +
Sbjct: 81 DDELKAFDADFVK-VDQATLFDLILAANYLNIKTLLDLTCQTVADMIKGKTPEEIRKTFN 139
Query: 216 ----FTKEEEASLREEMPWAFE 163
FT EEE +R E WAFE
Sbjct: 140 IKNDFTPEEEEEVRRENQWAFE 161
>ref|NP_565296.1| E3 ubiquitin ligase SCF complex subunit SKP1/ASK1 (At14), putative;
protein id: At2g03170.1 [Arabidopsis thaliana]
gi|7488338|pir||T02709 probable kinetechore (Skp1p-like)
protein [imported] - Arabidopsis thaliana
gi|3548813|gb|AAC34485.1| E3 ubiquitin ligase SCF
complex subunit SKP1/ASK1 (At14), putative [Arabidopsis
thaliana]
Length = 149
Score = 77.8 bits (190), Expect = 7e-14
Identities = 45/129 (34%), Positives = 71/129 (54%), Gaps = 16/129 (12%)
Frame = -2
Query: 501 SLSKEMKAVEIFIDDDDKAFIIPLPEVSSQDLVKIIEYCKQRRTAANAKDF---DAEFAK 331
++++++K VE I+DD +PL V+ + L ++EYCK+ + +F D EF K
Sbjct: 21 AVARKLKIVEHMIEDDCVVTEVPLQNVTGKILSIVVEYCKKHVVDEESDEFKTWDEEFMK 80
Query: 330 ALNNKELLSLIAAANYLNMADLLDLLSQCIVDRV*NTI-------------FTKEEEASL 190
+ + L+ AANYLN+ LLDL +Q + DR+ + FT EEEA++
Sbjct: 81 KFDQPTVFQLLLAANYLNIKGLLDLSAQTVADRIKDKTPEEIREIFNIENDFTPEEEAAV 140
Query: 189 REEMPWAFE 163
R+E WAFE
Sbjct: 141 RKENAWAFE 149
>gb|AAD34458.1| Skp1 [Medicago sativa]
Length = 153
Score = 76.6 bits (187), Expect = 2e-13
Identities = 50/135 (37%), Positives = 71/135 (52%), Gaps = 22/135 (16%)
Frame = -2
Query: 501 SLSKEMKAVEIFIDDDDKAFIIPLPEVSSQDLVKIIEYCKQRRTAANAKD---------F 349
+++ E + ++ I+DD IPLP V+S+ L K+IEYCK+ AA A+D +
Sbjct: 20 AVALESQTIKHMIEDDCADSGIPLPNVTSKILAKVIEYCKKHVDAAAAEDKPNEDELKSW 79
Query: 348 DAEFAKALNNKELLSLIAAANYLNMADLLDLLSQCIVDRV*NTI-------------FTK 208
D+EF K ++ L LI AANYLN+ LLDL Q + D + F+
Sbjct: 80 DSEFVK-VDQATLFDLILAANYLNIKSLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFSP 138
Query: 207 EEEASLREEMPWAFE 163
EEE +R E WAFE
Sbjct: 139 EEEEEVRRENQWAFE 153
>dbj|BAB85607.1| kinetochore protein [Brassica juncea]
Length = 160
Score = 73.2 bits (178), Expect = 2e-12
Identities = 50/141 (35%), Positives = 70/141 (49%), Gaps = 28/141 (19%)
Frame = -2
Query: 501 SLSKEMKAVEIFIDDDDKAFIIPLPEVSSQDLVKIIEYCKQRRTAA-------------- 364
+++ E + + ++DD IPLP V+S+ L K+IEYCK+ AA
Sbjct: 21 AVALESQTIAHMVEDDCVDNGIPLPNVTSKILAKVIEYCKKHVDAAASKTEAVDGGASSD 80
Query: 363 -NAKDFDAEFAKALNNKELLSLIAAANYLNMADLLDLLSQCIVDRV*NTI---------- 217
+ K +DAEF K ++ L LI AANYLN+ +LLDL Q + D +
Sbjct: 81 DDLKAWDAEFMK-IDQATLFELILAANYLNIKNLLDLTCQTVADMIKGKTPEEIRTTFNI 139
Query: 216 ---FTKEEEASLREEMPWAFE 163
FT EEE +R E WAFE
Sbjct: 140 KNDFTPEEEEEVRRENQWAFE 160
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 440,114,635
Number of Sequences: 1393205
Number of extensions: 9434079
Number of successful extensions: 38300
Number of sequences better than 10.0: 161
Number of HSP's better than 10.0 without gapping: 34305
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 37716
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 16731298976
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
