>KMC014774A_C01 KMC014774A_c01
gaaaacaaatatatCTGCTATTTACTTTTTATTAAAGTCATCAAACAAGATACACCCCTA
CATTAGACGTGTTCTAATGTCAATCTACCATTGAAAATGCTGTCCAAAGTGGCCCTTCTA
GACAAGCTGGGAAGCCCAGAAACTACACATATAAATGCTCTTGGATCAGGTGAAACAAAA
ATGGTGAATCACATATTGACAGGCAGTTCCACAACTAGGGGGCTATCCATACCTCAACTT
CAGAGTTGCAAATCAGATTTTAAACTACTCTCTTGTGTTTGACAGGCAGTTCCACAACTA
GGGGGCTATCCATACCGCAACTTCAGAGTTGCAGATCTAGATTTTAAACTACTCTCTTAC
GTCCGTCTCCTGACCCGCCTGCCATTTGGATGAGCTGATTCAGCGGCAAACGCATCAGCT
AAATCTTGCCCGTAAATGCTGCTGCTACTTCTCTTCCTGCTCAGGTTCTGGCTT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC014774A_C01 KMC014774A_c01
         (474 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_357323.1| AGR_L_3070p [Agrobacterium tumefaciens] gi|1793...    32  3.4
sp|P18695|TBG_EMENI Tubulin gamma chain (Gamma tubulin) gi|83723...    31  5.8
sp|P53377|TBG_NEUCR Tubulin gamma chain (Gamma tubulin) gi|14309...    31  7.6
gb|AAO52742.1| adhesion molecule VCAM-1 [Oryctolagus cuniculus]        31  7.6

>ref|NP_357323.1| AGR_L_3070p [Agrobacterium tumefaciens]
           gi|17936993|ref|NP_533782.1| ABC transporter, substrate
           binding protein [sugar] [Agrobacterium tumefaciens str.
           C58 (U. Washington)] gi|25519487|pir||B98323
           hypothetical protein AGR_L_3070 [imported] -
           Agrobacterium tumefaciens (strain C58, Cereon)
           gi|25524068|pir||AD2960 hypothetical protein Atu3282
           [imported] - Agrobacterium tumefaciens (strain C58,
           Dupont) gi|15160099|gb|AAK90108.1| AGR_L_3070p
           [Agrobacterium tumefaciens str. C58 (Cereon)]
           gi|17741666|gb|AAL44098.1| ABC transporter, substrate
           binding protein [sugar] [Agrobacterium tumefaciens str.
           C58 (U. Washington)]
          Length = 416

 Score = 32.0 bits (71), Expect = 3.4
 Identities = 24/80 (30%), Positives = 40/80 (50%)
 Frame = +3

Query: 234 STSELQIRF*TTLLCLTGSSTTRGLSIPQLQSCRSRF*TTLLRPSPDPPAIWMS*FSGKR 413
           +TSE+Q ++  T++    SS    L +  L          ++RP+    A W S FSGK 
Sbjct: 69  NTSEMQAQYLNTVMSAKDSS----LDVLMLD---------VIRPAQFATAGWTSDFSGKD 115

Query: 414 IS*ILPVNAAATSLPAQVLA 473
           +S  LP  A A ++  +++A
Sbjct: 116 LSAYLPTYAEANTVNGKIVA 135

>sp|P18695|TBG_EMENI Tubulin gamma chain (Gamma tubulin) gi|83723|pir||S03916 tubulin
           gamma chain - Emericella nidulans
           gi|2363|emb|CAA33507.1| gamma-tubulin [Emericella
           nidulans] gi|226345|prf||1507308A gamma tubulin
          Length = 454

 Score = 31.2 bits (69), Expect = 5.8
 Identities = 14/40 (35%), Positives = 20/40 (50%)
 Frame = +3

Query: 27  FY*SHQTRYTPTLDVF*CQSTIENAVQSGPSRQAGKPRNY 146
           FY S  TRY P   +   +  + N +QSGP +    P N+
Sbjct: 52  FYQSDDTRYIPRAILLDLEPRVLNGIQSGPYKNIYNPENF 91

>sp|P53377|TBG_NEUCR Tubulin gamma chain (Gamma tubulin) gi|1430903|emb|CAA66348.1|
           gamma-tubulin [Neurospora crassa]
           gi|28918686|gb|EAA28357.1| TUBULIN GAMMA CHAIN (GAMMA
           TUBULIN) [Neurospora crassa]
          Length = 461

 Score = 30.8 bits (68), Expect = 7.6
 Identities = 14/40 (35%), Positives = 20/40 (50%)
 Frame = +3

Query: 27  FY*SHQTRYTPTLDVF*CQSTIENAVQSGPSRQAGKPRNY 146
           FY S  TRY P   +   +  + N +Q+GP R    P N+
Sbjct: 52  FYQSDDTRYIPRSILIDLEPRVINTIQTGPYRNIYNPENF 91

>gb|AAO52742.1| adhesion molecule VCAM-1 [Oryctolagus cuniculus]
          Length = 739

 Score = 30.8 bits (68), Expect = 7.6
 Identities = 23/63 (36%), Positives = 29/63 (45%), Gaps = 11/63 (17%)
 Frame = -2

Query: 188 FTIFVSPDPR---------AFICVVSGL--PSLSRRATLDSIFNGRLTLEHV*CRGVSCL 42
           FT+ +SP PR            C V G   PS S R  +DS  NG++T E     G   L
Sbjct: 313 FTVEISPGPRIAAQIGDPVVLTCSVRGCETPSFSWRTQIDSPLNGQVTSE-----GTKSL 367

Query: 41  MTL 33
           +TL
Sbjct: 368 LTL 370

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 388,046,075
Number of Sequences: 1393205
Number of extensions: 7830137
Number of successful extensions: 15180
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 14710
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 15169
length of database: 448,689,247
effective HSP length: 113
effective length of database: 291,257,082
effective search space used: 12815311608
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MF076d08_f BP032326 1 474 2 MWL074c09_f AV769909 15 248

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