Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC014680A_C01 KMC014680A_c01
(600 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAM12530.1|AF462633_1 isoflavone synthase [Pueraria montana v... 271 5e-72
emb|CAB50768.1| cytochrome P450 [Cicer arietinum] 267 7e-71
dbj|BAA93632.1| cytochrome P450 [Lotus japonicus] 267 9e-71
dbj|BAA76380.1| cytochrome P450 [Glycyrrhiza echinata] 266 2e-70
gb|AAF34527.1|AF195806_1 isoflavone synthase 1 [Vigna radiata] 265 3e-70
>gb|AAM12530.1|AF462633_1 isoflavone synthase [Pueraria montana var. lobata]
Length = 521
Score = 271 bits (693), Expect = 5e-72
Identities = 132/160 (82%), Positives = 146/160 (90%), Gaps = 2/160 (1%)
Frame = -3
Query: 598 TFRMHPPLPVVKRKCVQECELNGYVIPEGALILFNVWAVQRDPKYWEGPSEFRPERFLT- 422
TFRMHPPLPVVKRKCV+ECE+NG+VIPEGALILFNVWAV RDPKYW+ P EFRPERFL
Sbjct: 362 TFRMHPPLPVVKRKCVEECEVNGFVIPEGALILFNVWAVGRDPKYWDRPLEFRPERFLES 421
Query: 421 -AEGGATSIDLRGQNFELLPFGSGRRMCPGVNLATAGMATLLASVIQCFDLQVVGQKGKL 245
AEG IDLRGQ+F+LLPFGSGRRMCPGVNLAT+GMATLLAS+IQCFDLQVVG KG++
Sbjct: 422 GAEGEVGPIDLRGQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCFDLQVVGPKGEI 481
Query: 244 LKGSDAKVSMEESPGLTVPRAHNLMCVPLARTNVTSELLS 125
LKG+DAKVSMEES GLTVPRAHNL CVPLART+V ++LLS
Sbjct: 482 LKGNDAKVSMEESAGLTVPRAHNLRCVPLARTSVAAKLLS 521
>emb|CAB50768.1| cytochrome P450 [Cicer arietinum]
Length = 525
Score = 267 bits (683), Expect = 7e-71
Identities = 127/161 (78%), Positives = 143/161 (87%), Gaps = 2/161 (1%)
Frame = -3
Query: 598 TFRMHPPLPVVKRKCVQECELNGYVIPEGALILFNVWAVQRDPKYWEGPSEFRPERFL-- 425
TFRMHPPLPVVKRKC +ECE+NGYVIPEGAL+LFNVWAV RDPKYW+ P EFRPERFL
Sbjct: 365 TFRMHPPLPVVKRKCTEECEINGYVIPEGALVLFNVWAVGRDPKYWDRPLEFRPERFLEN 424
Query: 424 TAEGGATSIDLRGQNFELLPFGSGRRMCPGVNLATAGMATLLASVIQCFDLQVVGQKGKL 245
EG A SIDLRGQ+F+LLPFGSGRRMCPGVNLATAGMATLL+SV+QCF+LQV G G++
Sbjct: 425 AGEGDAGSIDLRGQHFQLLPFGSGRRMCPGVNLATAGMATLLSSVLQCFELQVAGPNGQI 484
Query: 244 LKGSDAKVSMEESPGLTVPRAHNLMCVPLARTNVTSELLSS 122
LKG+DAKVSM+E PGLTVPRAHNL+CVPLAR ++LLSS
Sbjct: 485 LKGADAKVSMDERPGLTVPRAHNLVCVPLARPGAAAKLLSS 525
>dbj|BAA93632.1| cytochrome P450 [Lotus japonicus]
Length = 518
Score = 267 bits (682), Expect = 9e-71
Identities = 130/159 (81%), Positives = 143/159 (89%)
Frame = -3
Query: 598 TFRMHPPLPVVKRKCVQECELNGYVIPEGALILFNVWAVQRDPKYWEGPSEFRPERFLTA 419
TFRMHPPLPVVKRKCVQECELNGYVIPEGAL+LFNVWAVQRDPKYW+ P EFRPERFL
Sbjct: 364 TFRMHPPLPVVKRKCVQECELNGYVIPEGALVLFNVWAVQRDPKYWKTPLEFRPERFLEE 423
Query: 418 EGGATSIDLRGQNFELLPFGSGRRMCPGVNLATAGMATLLASVIQCFDLQVVGQKGKLLK 239
IDL+GQ+FELLPFGSGRRMCPGVNLATAGMATLL+SVIQCF+LQVVG KG++LK
Sbjct: 424 ----ADIDLKGQHFELLPFGSGRRMCPGVNLATAGMATLLSSVIQCFELQVVGPKGQILK 479
Query: 238 GSDAKVSMEESPGLTVPRAHNLMCVPLARTNVTSELLSS 122
GSDAK +MEE GLTVPRA++LMCVPLAR+ V +ELLSS
Sbjct: 480 GSDAKANMEERAGLTVPRANSLMCVPLARSKVAAELLSS 518
>dbj|BAA76380.1| cytochrome P450 [Glycyrrhiza echinata]
Length = 523
Score = 266 bits (679), Expect = 2e-70
Identities = 128/160 (80%), Positives = 144/160 (90%), Gaps = 1/160 (0%)
Frame = -3
Query: 598 TFRMHPPLPVVKRKCVQECELNGYVIPEGALILFNVWAVQRDPKYWEGPSEFRPERFLTA 419
TFRMHPPLPVVKRKCVQECE++GYVIPEGALILFNVWAV RDPKYW+ P+EFRPERFL
Sbjct: 364 TFRMHPPLPVVKRKCVQECEVDGYVIPEGALILFNVWAVGRDPKYWDRPTEFRPERFLEN 423
Query: 418 EG-GATSIDLRGQNFELLPFGSGRRMCPGVNLATAGMATLLASVIQCFDLQVVGQKGKLL 242
G G ++DLRGQ+F+LLPFGSGRRMCPGVNLATAGMATLLASVIQCFDL VVG +GK+L
Sbjct: 424 VGEGDQAVDLRGQHFQLLPFGSGRRMCPGVNLATAGMATLLASVIQCFDLSVVGPQGKIL 483
Query: 241 KGSDAKVSMEESPGLTVPRAHNLMCVPLARTNVTSELLSS 122
KG+DAKVSMEE GLTVPRAHNL+CVP+AR++ +L SS
Sbjct: 484 KGNDAKVSMEERAGLTVPRAHNLICVPVARSSAVPKLFSS 523
>gb|AAF34527.1|AF195806_1 isoflavone synthase 1 [Vigna radiata]
Length = 522
Score = 265 bits (678), Expect = 3e-70
Identities = 128/160 (80%), Positives = 143/160 (89%), Gaps = 2/160 (1%)
Frame = -3
Query: 598 TFRMHPPLPVVKRKCVQECELNGYVIPEGALILFNVWAVQRDPKYWEGPSEFRPERFLT- 422
TFRMHPPLPVVKRKC +ECE+NGYVIPEGALILFNVW V RDPKYW+ PSEFRPERFL
Sbjct: 362 TFRMHPPLPVVKRKCTEECEINGYVIPEGALILFNVWQVGRDPKYWDRPSEFRPERFLET 421
Query: 421 -AEGGATSIDLRGQNFELLPFGSGRRMCPGVNLATAGMATLLASVIQCFDLQVVGQKGKL 245
AEG A +DLRGQ+F+LLPFGSGRRMCPGVNLAT+GMATLLAS+IQCFDLQV+G +G++
Sbjct: 422 GAEGEARPLDLRGQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCFDLQVLGPQGQI 481
Query: 244 LKGSDAKVSMEESPGLTVPRAHNLMCVPLARTNVTSELLS 125
LKG DAKVSMEE GLTVPRAH+L+CVPLAR V S+LLS
Sbjct: 482 LKGGDAKVSMEERAGLTVPRAHSLVCVPLARIGVASKLLS 521
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 570,903,592
Number of Sequences: 1393205
Number of extensions: 13176983
Number of successful extensions: 39948
Number of sequences better than 10.0: 2901
Number of HSP's better than 10.0 without gapping: 35698
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 37602
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 23426109484
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
