>KMC014657A_C01 KMC014657A_c01
gcatatacaattttcatacactaatacaatattcatacactaacacaaagaagaatccaa
gtttgaagataatTTTTTATTAAAAGAAACAACGTATCTTTATATTGGTGAAGCTTTTAA
GACTACACAGAAATATTTGCTGGGTACTCTTTTGGATGATTTTTTACAGTCTCCTTCAGC
CATTGCTCCTGCTCACGGAGATTGGATGGAGGGACATCATAAACATCACTAAAAATGTCC
CCAAGCGGAGGTTTTTCTACACTCTCCGCTACCTGAATTGTATGCAGAAGCTGCTGTCTC
AAACTGTTTCTAAGCTCTGACTCAGCCACAGCATTCCACCAACCATTCCTTTCTATCCAC
TTTCTAAATTTAGCCACAGGATCGCGTGCCTGTCTCCACCATTCAATCTCACTGGCTGGA
CGATACTTGGTTGAATCATCCGATGTGGAGTGGTGTCCAACTCTATAAGTAAGAGCTTCT
ATTAGAACTGGTCTCTCTTCACTAATTGCCATTTGACGAGCAGC

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC014657A_C01 KMC014657A_c01
         (524 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_568209.1| branched-chain alpha keto-acid dehydrogenase E1...   188  3e-47
emb|CAC05456.1| branched-chain alpha keto-acid dehydrogenase E1 ...   188  3e-47
pir||T51858 branched-chain alpha keto-acid dehydrogenase E1 alph...   178  4e-44
ref|NP_173562.1| branched-chain alpha keto-acid dehydrogenase, p...   178  4e-44
pir||T06589 3-methyl-2-oxobutanoate dehydrogenase (lipoamide) (E...   169  2e-41

>ref|NP_568209.1| branched-chain alpha keto-acid dehydrogenase E1 alpha subunit-like
           protein; protein id: At5g09300.1, supported by cDNA:
           3693. [Arabidopsis thaliana] gi|28393751|gb|AAO42286.1|
           putative branched-chain alpha keto-acid dehydrogenase E1
           alpha subunit [Arabidopsis thaliana]
           gi|28973423|gb|AAO64036.1| putative branched-chain alpha
           keto-acid dehydrogenase E1 alpha subunit [Arabidopsis
           thaliana]
          Length = 472

 Score =  188 bits (478), Expect = 3e-47
 Identities = 80/130 (61%), Positives = 111/130 (84%)
 Frame = -1

Query: 521 ARQMAISEERPVLIEALTYRVGHHSTSDDSTKYRPASEIEWWRQARDPVAKFRKWIERNG 342
           AR+MAI E+RP+LIEALTYRVGHHSTSDDST+YR A EIEWW +AR+P+++FR WIE NG
Sbjct: 341 AREMAIREQRPILIEALTYRVGHHSTSDDSTRYRSAGEIEWWNKARNPLSRFRTWIESNG 400

Query: 341 WWNAVAESELRNSLRQQLLHTIQVAESVEKPPLGDIFSDVYDVPPSNLREQEQWLKETVK 162
           WW+   ES+LR+ +++++L  ++VAE  EKP L ++FSDVYDVPPSNLREQE  +++T+ 
Sbjct: 401 WWSDKTESDLRSRIKKEMLEALRVAEKTEKPNLQNMFSDVYDVPPSNLREQELLVRQTIN 460

Query: 161 NHPKEYPANI 132
           +HP++YP+++
Sbjct: 461 SHPQDYPSDV 470

>emb|CAC05456.1| branched-chain alpha keto-acid dehydrogenase E1 alpha subunit-like
           protein [Arabidopsis thaliana]
          Length = 414

 Score =  188 bits (478), Expect = 3e-47
 Identities = 80/130 (61%), Positives = 111/130 (84%)
 Frame = -1

Query: 521 ARQMAISEERPVLIEALTYRVGHHSTSDDSTKYRPASEIEWWRQARDPVAKFRKWIERNG 342
           AR+MAI E+RP+LIEALTYRVGHHSTSDDST+YR A EIEWW +AR+P+++FR WIE NG
Sbjct: 283 AREMAIREQRPILIEALTYRVGHHSTSDDSTRYRSAGEIEWWNKARNPLSRFRTWIESNG 342

Query: 341 WWNAVAESELRNSLRQQLLHTIQVAESVEKPPLGDIFSDVYDVPPSNLREQEQWLKETVK 162
           WW+   ES+LR+ +++++L  ++VAE  EKP L ++FSDVYDVPPSNLREQE  +++T+ 
Sbjct: 343 WWSDKTESDLRSRIKKEMLEALRVAEKTEKPNLQNMFSDVYDVPPSNLREQELLVRQTIN 402

Query: 161 NHPKEYPANI 132
           +HP++YP+++
Sbjct: 403 SHPQDYPSDV 412

>pir||T51858 branched-chain alpha keto-acid dehydrogenase E1 alpha chain
           [imported] - Arabidopsis thaliana (fragment)
           gi|3822223|gb|AAC69851.1| branched-chain alpha keto-acid
           dehydrogenase E1 alpha subunit [Arabidopsis thaliana]
          Length = 472

 Score =  178 bits (451), Expect = 4e-44
 Identities = 81/133 (60%), Positives = 104/133 (77%)
 Frame = -1

Query: 524 AARQMAISEERPVLIEALTYRVGHHSTSDDSTKYRPASEIEWWRQARDPVAKFRKWIERN 345
           +AR+MA++E+RPVLIE +TYRVGHHSTSDDSTKYR A EI++W+ +R+PV +FRKW+E N
Sbjct: 340 SAREMAVTEQRPVLIEMMTYRVGHHSTSDDSTKYRAADEIQYWKMSRNPVNRFRKWVEDN 399

Query: 344 GWWNAVAESELRNSLRQQLLHTIQVAESVEKPPLGDIFSDVYDVPPSNLREQEQWLKETV 165
           GWW+   ES+LR++ R+QLL  IQ AE  EK PL ++F+DVYDV P NL EQE  LKE V
Sbjct: 400 GWWSEEDESKLRSNARKQLLQAIQAAEKWEKQPLTELFNDVYDVKPKNLEEQELGLKELV 459

Query: 164 KNHPKEYPANISV 126
           K  P++YP    V
Sbjct: 460 KKQPQDYPPGFHV 472

>ref|NP_173562.1| branched-chain alpha keto-acid dehydrogenase, putative; protein id:
           At1g21400.1, supported by cDNA: gi_20466297 [Arabidopsis
           thaliana] gi|25284423|pir||A86347 branched-chain alpha
           keto-acid dehydrogenase E1-alpha subunit [imported] -
           Arabidopsis thaliana gi|9454571|gb|AAF87894.1|AC015447_4
           branched-chain alpha keto-acid dehydrogenase E1 - alpha
           subunit [Arabidopsis thaliana]
           gi|20466298|gb|AAM20466.1| branched-chain alpha
           keto-acid dehydrogenase, putative [Arabidopsis thaliana]
           gi|23198122|gb|AAN15588.1| branched-chain alpha
           keto-acid dehydrogenase, putative [Arabidopsis thaliana]
          Length = 472

 Score =  178 bits (451), Expect = 4e-44
 Identities = 81/133 (60%), Positives = 104/133 (77%)
 Frame = -1

Query: 524 AARQMAISEERPVLIEALTYRVGHHSTSDDSTKYRPASEIEWWRQARDPVAKFRKWIERN 345
           +AR+MA++E+RPVLIE +TYRVGHHSTSDDSTKYR A EI++W+ +R+PV +FRKW+E N
Sbjct: 340 SAREMAVTEQRPVLIEMMTYRVGHHSTSDDSTKYRAADEIQYWKMSRNPVNRFRKWVEDN 399

Query: 344 GWWNAVAESELRNSLRQQLLHTIQVAESVEKPPLGDIFSDVYDVPPSNLREQEQWLKETV 165
           GWW+   ES+LR++ R+QLL  IQ AE  EK PL ++F+DVYDV P NL EQE  LKE V
Sbjct: 400 GWWSEEDESKLRSNARKQLLQAIQAAEKWEKQPLTELFNDVYDVKPKNLEEQELGLKELV 459

Query: 164 KNHPKEYPANISV 126
           K  P++YP    V
Sbjct: 460 KKQPQDYPPGFHV 472

>pir||T06589 3-methyl-2-oxobutanoate dehydrogenase (lipoamide) (EC 1.2.4.4)
           E1-alpha chain precursor, mitochondrial - tomato
           gi|1945277|emb|CAB08111.1| branched chain alpha-keto
           acid dehydrogenase E1-alpha subunit [Lycopersicon
           esculentum]
          Length = 456

 Score =  169 bits (427), Expect = 2e-41
 Identities = 76/130 (58%), Positives = 102/130 (78%)
 Frame = -1

Query: 524 AARQMAISEERPVLIEALTYRVGHHSTSDDSTKYRPASEIEWWRQARDPVAKFRKWIERN 345
           AAR MAISEERP+L+EA+TYRVGHHSTSDDSTKYRP +EIE+W+ AR P+  FRK I+RN
Sbjct: 324 AARTMAISEERPILVEAMTYRVGHHSTSDDSTKYRPTNEIEYWKTARCPINLFRKHIQRN 383

Query: 344 GWWNAVAESELRNSLRQQLLHTIQVAESVEKPPLGDIFSDVYDVPPSNLREQEQWLKETV 165
           GWW+ V ESEL   ++ Q+L  I+ AE +EKP L ++FSDVY+    NL+EQE+ +++ +
Sbjct: 384 GWWSDVQESELYTHVKNQVLEAIKTAEGMEKPALTELFSDVYEKMTPNLQEQERCIRDAI 443

Query: 164 KNHPKEYPAN 135
             +PK+YP +
Sbjct: 444 HKYPKDYPTD 453

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 453,504,822
Number of Sequences: 1393205
Number of extensions: 9884771
Number of successful extensions: 26246
Number of sequences better than 10.0: 243
Number of HSP's better than 10.0 without gapping: 25471
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 26169
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 17019769648
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MWL057a10_f AV769564 1 524 2 MFB027e07_f BP035971 74 524

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