>KMC014519A_C01 KMC014519A_c01
atctaaaattctagatcacctttccattcatgaaaaggatactcaaggagattgtcaact
aaaatactggtatggcttagGGGAAAAAACTAGAATACTGCTACTCTATGAATTATCGAA
CACCTCTCTTAAAATTATTTAATTTTCACTTTTTTTCTTCTTTTTGGAATAAATTTTTAT
TTGAACATGATGTTCTGCTCAATGCACTGCAGTACCATGGGAGCTACAAAAGCAGCAAAA
GAATACTTTTTTTTGTTGGTCATCTACTTCTTTTCCTTTTAAGAGCTTGAAGAACAGATT
GTGCTGTGCAGTAAGCAATTGCTTGAACAGTGATCATTGGATTCACACCCAAAGCTGTGG
GGAAAACACTTGAATCTGCCAAGTAAAGGTCCTCCACTTCCCATGTCTCCCCAGTCTGAT
TCACCACTGAATTCTTCGGATTAGAACCCATCCGGCAGCTCCCCATCTGATGCGCCGAGC
ACAATGGTGTTTTAAGGTCTGTCAGTGACCTTGCACTTTCCTCTTTGATAAATTTCTCAA
GTTCATGAACACTTGCTTGCTTTACATTCAAGCTCTTTCCCTTGTTATGATGAGTTCCAA
TCTCTTCAGCTCCAGCCGCTGCCAGAATTCTCAGCACCTTGTCAATTCCATTCTGTAAAT
GCTCTTCATCTGCATTTTCCATCTGATAACTGATGTGGCTTCGCGAATGCACTGTCCCTG
A

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC014519A_C01 KMC014519A_c01
         (721 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_193673.1| putative protein; protein id: At4g19380.1 [Arab...   201  9e-51
gb|AAL31049.1|AC078893_12 putative oxidase [Oryza sativa]             141  8e-33
gb|AAL31021.1|AC078948_5 putative alcohol oxidase [Oryza sativa]      141  8e-33
emb|CAC87643.1| alcohol oxidase [Arabidopsis thaliana]                131  8e-30
ref|NP_566729.1| expressed protein; protein id: At3g23410.1, sup...   131  8e-30

>ref|NP_193673.1| putative protein; protein id: At4g19380.1 [Arabidopsis thaliana]
           gi|7487715|pir||T05821 hypothetical protein T5K18.160 -
           Arabidopsis thaliana gi|3080368|emb|CAA18625.1| putative
           protein [Arabidopsis thaliana]
           gi|7268733|emb|CAB78940.1| putative protein [Arabidopsis
           thaliana]
          Length = 678

 Score =  201 bits (511), Expect = 9e-51
 Identities = 92/151 (60%), Positives = 125/151 (81%)
 Frame = -1

Query: 721 SGTVHSRSHISYQMENADEEHLQNGIDKVLRILAAAGAEEIGTHHNKGKSLNVKQASVHE 542
           +GT+ S+++I Y + + DEE L+NG+++VL+ILAAAGAEEIGTHH++G+SLNV+ AS  E
Sbjct: 528 TGTIDSKTYIDYNLNDEDEESLKNGLERVLKILAAAGAEEIGTHHSEGRSLNVRTASSLE 587

Query: 541 LEKFIKEESARSLTDLKTPLCSAHQMGSCRMGSNPKNSVVNQTGETWEVEDLYLADSSVF 362
           +E+F++EES++ L DL   +CSAHQMGSCRMG  P+ S V  TGETWEVE L++AD+SVF
Sbjct: 588 IERFVREESSKPLKDLSGQICSAHQMGSCRMGIRPEESAVRPTGETWEVERLFVADTSVF 647

Query: 361 PTALGVNPMITVQAIAYCTAQSVLQALKRKR 269
           PTALGVNPM+TVQ+IAYC   +V+  LK+K+
Sbjct: 648 PTALGVNPMVTVQSIAYCIGLNVVDVLKKKK 678

>gb|AAL31049.1|AC078893_12 putative oxidase [Oryza sativa]
          Length = 595

 Score =  141 bits (356), Expect = 8e-33
 Identities = 69/152 (45%), Positives = 97/152 (63%), Gaps = 5/152 (3%)
 Frame = -1

Query: 721 SGTVHSRSHISYQMENADEEHLQNGIDKVLRILAAAGAEEIGTHHNKGKSLNVKQASVHE 542
           +G+V     + Y     D E L+ G+ + LRIL AAGA E+GTH + G  L  K A   +
Sbjct: 437 AGSVDGEGRVRYAPSRDDAEELRAGLRRALRILVAAGAAEVGTHRSDGARLRCKGARDAD 496

Query: 541 LEKFIKEESAR-----SLTDLKTPLCSAHQMGSCRMGSNPKNSVVNQTGETWEVEDLYLA 377
           +E F+ E +       S TD  + LCSAHQMGSCRMG++P++  V+  GE+WE E LY+ 
Sbjct: 497 VEAFLDEVTVEKGPMHSTTDKWSVLCSAHQMGSCRMGASPRDGAVDVAGESWEAEGLYVC 556

Query: 376 DSSVFPTALGVNPMITVQAIAYCTAQSVLQAL 281
           D S+ PTA+GVNPMIT+Q+IAYC A+ +  ++
Sbjct: 557 DGSLLPTAVGVNPMITIQSIAYCVAKGIADSM 588

>gb|AAL31021.1|AC078948_5 putative alcohol oxidase [Oryza sativa]
          Length = 590

 Score =  141 bits (356), Expect = 8e-33
 Identities = 69/152 (45%), Positives = 97/152 (63%), Gaps = 5/152 (3%)
 Frame = -1

Query: 721 SGTVHSRSHISYQMENADEEHLQNGIDKVLRILAAAGAEEIGTHHNKGKSLNVKQASVHE 542
           +G+V     + Y     D E L+ G+ + LRIL AAGA E+GTH + G  L  K A   +
Sbjct: 432 AGSVDGEGRVRYAPSRDDAEELRAGLRRALRILVAAGAAEVGTHRSDGARLRCKGARDAD 491

Query: 541 LEKFIKEESAR-----SLTDLKTPLCSAHQMGSCRMGSNPKNSVVNQTGETWEVEDLYLA 377
           +E F+ E +       S TD  + LCSAHQMGSCRMG++P++  V+  GE+WE E LY+ 
Sbjct: 492 VEAFLDEVTVEKGPMHSTTDKWSVLCSAHQMGSCRMGASPRDGAVDVAGESWEAEGLYVC 551

Query: 376 DSSVFPTALGVNPMITVQAIAYCTAQSVLQAL 281
           D S+ PTA+GVNPMIT+Q+IAYC A+ +  ++
Sbjct: 552 DGSLLPTAVGVNPMITIQSIAYCVAKGIADSM 583

>emb|CAC87643.1| alcohol oxidase [Arabidopsis thaliana]
          Length = 678

 Score =  131 bits (330), Expect = 8e-30
 Identities = 62/151 (41%), Positives = 98/151 (64%), Gaps = 4/151 (2%)
 Frame = -1

Query: 721 SGTVHSRSHISYQMENADEEHLQNGIDKVLRILAAAGAEEIGTHHNKGKSLNVKQASVHE 542
           SG V +   I+Y ++  D ++L+ G+ + LRIL AAGAEE+GTH + G+ L  K  + + 
Sbjct: 522 SGEVKTEGRINYTVDKTDRDNLKAGLRESLRILIAAGAEEVGTHRSDGQRLICKGVNENS 581

Query: 541 LEKFIK----EESARSLTDLKTPLCSAHQMGSCRMGSNPKNSVVNQTGETWEVEDLYLAD 374
           +++F+     EE A+ +T+      SAHQMGSCR+G N K   ++  GE+WE E L++ D
Sbjct: 582 IQEFLDSVSTEEGAKGMTEKWNVYSSAHQMGSCRIGENEKEGAIDLNGESWEAEKLFVCD 641

Query: 373 SSVFPTALGVNPMITVQAIAYCTAQSVLQAL 281
           +S  P+A+GVNPMITV + AYC +  + +++
Sbjct: 642 ASALPSAVGVNPMITVMSTAYCISTRIAKSM 672

>ref|NP_566729.1| expressed protein; protein id: At3g23410.1, supported by cDNA:
            gi_13605691 [Arabidopsis thaliana]
            gi|11994326|dbj|BAB02285.1| contains similarity to long
            chain fatty alcohol oxidase~gene_id:MLM24.14 [Arabidopsis
            thaliana] gi|13605692|gb|AAK32839.1|AF361827_1
            AT3g23410/MLM24_23 [Arabidopsis thaliana]
            gi|27363420|gb|AAO11629.1| At3g23410/MLM24_23
            [Arabidopsis thaliana]
          Length = 746

 Score =  131 bits (330), Expect = 8e-30
 Identities = 62/151 (41%), Positives = 98/151 (64%), Gaps = 4/151 (2%)
 Frame = -1

Query: 721  SGTVHSRSHISYQMENADEEHLQNGIDKVLRILAAAGAEEIGTHHNKGKSLNVKQASVHE 542
            SG V +   I+Y ++  D ++L+ G+ + LRIL AAGAEE+GTH + G+ L  K  + + 
Sbjct: 590  SGEVKTEGRINYTVDKTDRDNLKAGLRESLRILIAAGAEEVGTHRSDGQRLICKGVNENS 649

Query: 541  LEKFIK----EESARSLTDLKTPLCSAHQMGSCRMGSNPKNSVVNQTGETWEVEDLYLAD 374
            +++F+     EE A+ +T+      SAHQMGSCR+G N K   ++  GE+WE E L++ D
Sbjct: 650  IQEFLDSVSTEEGAKGMTEKWNVYSSAHQMGSCRIGENEKEGAIDLNGESWEAEKLFVCD 709

Query: 373  SSVFPTALGVNPMITVQAIAYCTAQSVLQAL 281
            +S  P+A+GVNPMITV + AYC +  + +++
Sbjct: 710  ASALPSAVGVNPMITVMSTAYCISTRIAKSM 740

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 608,204,854
Number of Sequences: 1393205
Number of extensions: 13195634
Number of successful extensions: 40055
Number of sequences better than 10.0: 174
Number of HSP's better than 10.0 without gapping: 37740
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 39974
length of database: 448,689,247
effective HSP length: 120
effective length of database: 281,504,647
effective search space used: 33499052993
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MWL036h10_f AV769188 1 295 2 SPD085d12_f BP050787 167 717 3 SPDL003a07_f BP052149 180 389 4 SPDL074c06_f BP056570 194 721

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