Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC014348A_C01 KMC014348A_c01
(459 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAF63202.1|AF240679_1 poly(A)-binding protein [Cucumis sativus] 242 8e-65
gb|AAF66823.1|AF190655_1 poly(A)-binding protein [Nicotiana taba... 225 2e-59
gb|AAK30205.1|AF349964_1 poly(A)-binding protein [Daucus carota] 218 3e-57
ref|NP_195137.1| polyadenylate-binding protein 2 (PABP2); protei... 211 3e-54
gb|AAL86321.1| putative poly(A)-binding protein [Arabidopsis tha... 211 3e-54
>gb|AAF63202.1|AF240679_1 poly(A)-binding protein [Cucumis sativus]
Length = 649
Score = 242 bits (617), Expect(2) = 8e-65
Identities = 120/141 (85%), Positives = 128/141 (90%), Gaps = 3/141 (2%)
Frame = +2
Query: 2 PAPNAN---FVTTSLYVGDLDQNVNDSQLYDLFNQVGQVVSVRVCRDLTTRRSLGYGYVN 172
PA N VTTSLYVGDLD NV DSQLYDLFNQVGQVVSVRVCRDLT+RRSLGYGYVN
Sbjct: 17 PAANGGANQHVTTSLYVGDLDVNVTDSQLYDLFNQVGQVVSVRVCRDLTSRRSLGYGYVN 76
Query: 173 FSNPPDAARALDVLNFTPLNNRPIRVMYSHRDPSIRKSGTANIFIKNLDKAIDHKALHDT 352
+SNP DA+RALDVLNFTPLN PIRVMYSHRDPS+RKSG+ NIFIKNLDKAIDHKALHDT
Sbjct: 77 YSNPVDASRALDVLNFTPLNGNPIRVMYSHRDPSVRKSGSGNIFIKNLDKAIDHKALHDT 136
Query: 353 FSSFGSILSCKIATDASGQSK 415
FS+FGSILSCK+ATD+SGQSK
Sbjct: 137 FSAFGSILSCKVATDSSGQSK 157
Score = 74.3 bits (181), Expect = 5e-13
Identities = 44/144 (30%), Positives = 80/144 (55%), Gaps = 16/144 (11%)
Frame = +2
Query: 32 SLYVGDLDQNVNDSQLYDLFNQVGQVVSVRVCRDLTTRRSLGYGYVNFSNPPDAARALDV 211
+++V +L + ++ L ++F + G + SV V RD +S +G+VNF N DAAR+++
Sbjct: 209 NVFVKNLAETTSEEDLKNMFGEFGPITSVVVMRD-GEGKSKCFGFVNFENADDAARSVEA 267
Query: 212 LNFTPLNNRPIRV------------MYSHRDPSIRKSGT----ANIFIKNLDKAIDHKAL 343
LN ++ + V + S + S++++ AN+++KNLD +ID L
Sbjct: 268 LNGKKVDGKEWYVGKAQKKSEREVELKSRFEQSVKEAADKYQGANLYVKNLDDSIDDDKL 327
Query: 344 HDTFSSFGSILSCKIATDASGQSK 415
+ F+ FG+I SCK+ D +G S+
Sbjct: 328 KELFTGFGTITSCKVMRDPNGISR 351
Score = 68.2 bits (165), Expect = 4e-11
Identities = 38/132 (28%), Positives = 71/132 (53%), Gaps = 4/132 (3%)
Frame = +2
Query: 32 SLYVGDLDQNVNDSQLYDLFNQVGQVVSVRVCRDLTTRRSLGYGYVNFSNPPDAARALDV 211
++++ +LD+ ++ L+D F+ G ++S +V D ++ +S G+G+V F A +A++
Sbjct: 118 NIFIKNLDKAIDHKALHDTFSAFGSILSCKVATD-SSGQSKGFGFVQFDTEEAALKAIEK 176
Query: 212 LNFTPLNNRPIRV----MYSHRDPSIRKSGTANIFIKNLDKAIDHKALHDTFSSFGSILS 379
LN LN++ + V R+ K+ N+F+KNL + + L + F FG I S
Sbjct: 177 LNGMLLNDKQVFVGPFLRKQERESVSEKTKFNNVFVKNLAETTSEEDLKNMFGEFGPITS 236
Query: 380 CKIATDASGQSK 415
+ D G+SK
Sbjct: 237 VVVMRDGEGKSK 248
Score = 54.3 bits (129), Expect = 6e-07
Identities = 30/78 (38%), Positives = 48/78 (61%)
Frame = +2
Query: 32 SLYVGDLDQNVNDSQLYDLFNQVGQVVSVRVCRDLTTRRSLGYGYVNFSNPPDAARALDV 211
+LYV +LD +++D +L +LF G + S +V RD S G G+V FS+P +AARAL
Sbjct: 312 NLYVKNLDDSIDDDKLKELFTGFGTITSCKVMRD-PNGISRGSGFVAFSSPEEAARALAE 370
Query: 212 LNFTPLNNRPIRVMYSHR 265
+N + ++P+ V + R
Sbjct: 371 MNGRMIVSKPLYVALAQR 388
Score = 26.2 bits (56), Expect(2) = 8e-65
Identities = 10/14 (71%), Positives = 12/14 (85%)
Frame = +1
Query: 403 GPVQGYGFVQFDTE 444
G +G+GFVQFDTE
Sbjct: 154 GQSKGFGFVQFDTE 167
>gb|AAF66823.1|AF190655_1 poly(A)-binding protein [Nicotiana tabacum]
Length = 649
Score = 225 bits (573), Expect(2) = 2e-59
Identities = 107/133 (80%), Positives = 122/133 (91%)
Frame = +2
Query: 17 NFVTTSLYVGDLDQNVNDSQLYDLFNQVGQVVSVRVCRDLTTRRSLGYGYVNFSNPPDAA 196
N TSLYVGDLD NV DSQLYDLFNQ+GQVVSVRVCRDLT++RSLGYGYVN+ NP DAA
Sbjct: 23 NQFVTSLYVGDLDVNVTDSQLYDLFNQLGQVVSVRVCRDLTSQRSLGYGYVNYGNPQDAA 82
Query: 197 RALDVLNFTPLNNRPIRVMYSHRDPSIRKSGTANIFIKNLDKAIDHKALHDTFSSFGSIL 376
RAL+VLNFTPL+ +PIR+MYS+RDP+IR+SG NIFIKNLDKAIDHKALHDTFS+FG+IL
Sbjct: 83 RALEVLNFTPLHGKPIRIMYSNRDPTIRRSGNGNIFIKNLDKAIDHKALHDTFSAFGNIL 142
Query: 377 SCKIATDASGQSK 415
SCK+A D+SGQSK
Sbjct: 143 SCKVAVDSSGQSK 155
Score = 80.1 bits (196), Expect = 1e-14
Identities = 50/145 (34%), Positives = 79/145 (54%), Gaps = 16/145 (11%)
Frame = +2
Query: 29 TSLYVGDLDQNVNDSQLYDLFNQVGQVVSVRVCRDLTTRRSLGYGYVNFSNPPDAARALD 208
T+++V +L ++ + +L +F + G + SV V +D +S +G+VNF N DAARA++
Sbjct: 206 TNVFVKNLSESTLEEELRKIFGEFGAITSVAVMKD-EDGKSRCFGFVNFENAEDAARAVE 264
Query: 209 VLNFTPLNNRPIRV-----------MYSHR-----DPSIRKSGTANIFIKNLDKAIDHKA 340
LN L+N+ V HR ++ KS N++IKNLD +I +
Sbjct: 265 ALNGYKLDNKDWFVGRAQKKSEREMELKHRFEQSAKEAVDKSQGLNLYIKNLDDSISDEK 324
Query: 341 LHDTFSSFGSILSCKIATDASGQSK 415
L + FS +G+I SCK+ D SG SK
Sbjct: 325 LKELFSPYGTITSCKVMRDPSGVSK 349
Score = 71.6 bits (174), Expect = 4e-12
Identities = 38/132 (28%), Positives = 75/132 (56%), Gaps = 4/132 (3%)
Frame = +2
Query: 32 SLYVGDLDQNVNDSQLYDLFNQVGQVVSVRVCRDLTTRRSLGYGYVNFSNPPDAARALDV 211
++++ +LD+ ++ L+D F+ G ++S +V D ++ +S GYG+V + + A +A++
Sbjct: 116 NIFIKNLDKAIDHKALHDTFSAFGNILSCKVAVD-SSGQSKGYGFVQYDSEEAAQKAIEK 174
Query: 212 LNFTPLNNRPIRV----MYSHRDPSIRKSGTANIFIKNLDKAIDHKALHDTFSSFGSILS 379
LN LN++ + V RD ++ K+ N+F+KNL ++ + L F FG+I S
Sbjct: 175 LNGMLLNDKQVYVGPFVRKHERDMAVDKTRFTNVFVKNLSESTLEEELRKIFGEFGAITS 234
Query: 380 CKIATDASGQSK 415
+ D G+S+
Sbjct: 235 VAVMKDEDGKSR 246
Score = 56.2 bits (134), Expect = 2e-07
Identities = 29/78 (37%), Positives = 50/78 (63%)
Frame = +2
Query: 32 SLYVGDLDQNVNDSQLYDLFNQVGQVVSVRVCRDLTTRRSLGYGYVNFSNPPDAARALDV 211
+LY+ +LD +++D +L +LF+ G + S +V RD + S G G+V FSNP +A+RAL
Sbjct: 310 NLYIKNLDDSISDEKLKELFSPYGTITSCKVMRD-PSGVSKGSGFVAFSNPEEASRALSE 368
Query: 212 LNFTPLNNRPIRVMYSHR 265
+N + ++P+ V + R
Sbjct: 369 MNGKMVVSKPLYVALAQR 386
Score = 25.0 bits (53), Expect(2) = 2e-59
Identities = 9/14 (64%), Positives = 12/14 (85%)
Frame = +1
Query: 403 GPVQGYGFVQFDTE 444
G +GYGFVQ+D+E
Sbjct: 152 GQSKGYGFVQYDSE 165
>gb|AAK30205.1|AF349964_1 poly(A)-binding protein [Daucus carota]
Length = 658
Score = 218 bits (555), Expect(2) = 3e-57
Identities = 108/132 (81%), Positives = 118/132 (88%)
Frame = +2
Query: 20 FVTTSLYVGDLDQNVNDSQLYDLFNQVGQVVSVRVCRDLTTRRSLGYGYVNFSNPPDAAR 199
F TTSLYVGDLDQ+V DSQLYDLFNQVGQVVSVRVCRDL+T RSLGYGYVN+SN DA R
Sbjct: 37 FTTTSLYVGDLDQSVTDSQLYDLFNQVGQVVSVRVCRDLSTGRSLGYGYVNYSNQQDATR 96
Query: 200 ALDVLNFTPLNNRPIRVMYSHRDPSIRKSGTANIFIKNLDKAIDHKALHDTFSSFGSILS 379
A+DVLNFTPLNN+ IRV S RDP+ RKSG NIFIKNLDK+ID KALH+TFSSFG+I+S
Sbjct: 97 AIDVLNFTPLNNKTIRVSVSRRDPTDRKSGAGNIFIKNLDKSIDIKALHETFSSFGTIIS 156
Query: 380 CKIATDASGQSK 415
CKIATDASGQSK
Sbjct: 157 CKIATDASGQSK 168
Score = 79.0 bits (193), Expect = 2e-14
Identities = 48/144 (33%), Positives = 76/144 (52%), Gaps = 16/144 (11%)
Frame = +2
Query: 32 SLYVGDLDQNVNDSQLYDLFNQVGQVVSVRVCRDLTTRRSLGYGYVNFSNPPDAARALDV 211
++YV +L + ++ L F + G + SV V RD +S +G+VNF NP DAA+A+D
Sbjct: 220 NIYVKNLSDSTSEDDLRKTFGEYGTITSVVVMRD-ADGKSKCFGFVNFENPEDAAKAVDA 278
Query: 212 LNFTPLNNRPIRV----MYSHRDPSIR------------KSGTANIFIKNLDKAIDHKAL 343
LN +++ V S R+ ++ K N+++KNLD ID + L
Sbjct: 279 LNGKKFDDKEWYVGKAQKKSEREVELKSRFEQTVKEQVDKYQGVNLYVKNLDDTIDDEKL 338
Query: 344 HDTFSSFGSILSCKIATDASGQSK 415
+ FS +G+I SCK+ D SG S+
Sbjct: 339 KELFSEYGTITSCKVMRDPSGISR 362
Score = 72.4 bits (176), Expect = 2e-12
Identities = 38/132 (28%), Positives = 73/132 (54%), Gaps = 4/132 (3%)
Frame = +2
Query: 32 SLYVGDLDQNVNDSQLYDLFNQVGQVVSVRVCRDLTTRRSLGYGYVNFSNPPDAARALDV 211
++++ +LD++++ L++ F+ G ++S ++ D + +S GYG+V + + A A+D
Sbjct: 129 NIFIKNLDKSIDIKALHETFSSFGTIISCKIATD-ASGQSKGYGFVQYDSEEAAQTAIDK 187
Query: 212 LNFTPLNNRPIRV----MYSHRDPSIRKSGTANIFIKNLDKAIDHKALHDTFSSFGSILS 379
LN +N++ + V RD + K+ NI++KNL + L TF +G+I S
Sbjct: 188 LNGMLMNDKQVYVGVFLRKQDRDSEMSKTKFNNIYVKNLSDSTSEDDLRKTFGEYGTITS 247
Query: 380 CKIATDASGQSK 415
+ DA G+SK
Sbjct: 248 VVVMRDADGKSK 259
Score = 55.1 bits (131), Expect = 3e-07
Identities = 29/78 (37%), Positives = 49/78 (62%)
Frame = +2
Query: 32 SLYVGDLDQNVNDSQLYDLFNQVGQVVSVRVCRDLTTRRSLGYGYVNFSNPPDAARALDV 211
+LYV +LD ++D +L +LF++ G + S +V RD + S G G+V FS P +A+RAL
Sbjct: 323 NLYVKNLDDTIDDEKLKELFSEYGTITSCKVMRD-PSGISRGSGFVAFSTPEEASRALGE 381
Query: 212 LNFTPLNNRPIRVMYSHR 265
+N + ++P+ V + R
Sbjct: 382 MNGKMIVSKPLYVALAQR 399
Score = 25.0 bits (53), Expect(2) = 3e-57
Identities = 9/14 (64%), Positives = 12/14 (85%)
Frame = +1
Query: 403 GPVQGYGFVQFDTE 444
G +GYGFVQ+D+E
Sbjct: 165 GQSKGYGFVQYDSE 178
>ref|NP_195137.1| polyadenylate-binding protein 2 (PABP2); protein id: At4g34110.1
[Arabidopsis thaliana] gi|1171978|sp|P42731|PAB2_ARATH
Polyadenylate-binding protein 2 (Poly(A) binding protein
2) (PABP 2) gi|7439968|pir||T05425 polyadenylate-binding
protein F28A23.130 - Arabidopsis thaliana
gi|304109|gb|AAA61780.1| poly(A)-binding protein
gi|2911051|emb|CAA17561.1| poly(A)-binding protein
[Arabidopsis thaliana] gi|7270360|emb|CAB80128.1|
poly(A)-binding protein [Arabidopsis thaliana]
gi|26983870|gb|AAN86187.1| putative
polyadenylate-binding protein 2 (PABP2) [Arabidopsis
thaliana]
Length = 629
Score = 211 bits (538), Expect(2) = 3e-54
Identities = 101/137 (73%), Positives = 116/137 (83%)
Frame = +2
Query: 5 APNANFVTTSLYVGDLDQNVNDSQLYDLFNQVGQVVSVRVCRDLTTRRSLGYGYVNFSNP 184
A F TSLYVGDLD NV DSQL+D F Q+G VV+VRVCRDL TRRSLGYGYVNF+NP
Sbjct: 28 ATATQFGNTSLYVGDLDFNVTDSQLFDAFGQMGTVVTVRVCRDLVTRRSLGYGYVNFTNP 87
Query: 185 PDAARALDVLNFTPLNNRPIRVMYSHRDPSIRKSGTANIFIKNLDKAIDHKALHDTFSSF 364
DAARA+ LN+ PL +PIRVMYSHRDPS+R+SG NIFIKNLD++IDHKALHDTFSSF
Sbjct: 88 QDAARAIQELNYIPLYGKPIRVMYSHRDPSVRRSGAGNIFIKNLDESIDHKALHDTFSSF 147
Query: 365 GSILSCKIATDASGQSK 415
G+I+SCK+A D+SGQSK
Sbjct: 148 GNIVSCKVAVDSSGQSK 164
Score = 77.0 bits (188), Expect = 8e-14
Identities = 47/145 (32%), Positives = 79/145 (54%), Gaps = 16/145 (11%)
Frame = +2
Query: 29 TSLYVGDLDQNVNDSQLYDLFNQVGQVVSVRVCRDLTTRRSLGYGYVNFSNPPDAARALD 208
T++YV +L ++ D L + F + G++ S V +D +S G+G+VNF N DAARA++
Sbjct: 215 TNVYVKNLAESTTDDDLKNAFGEYGKITSAVVMKD-GEGKSKGFGFVNFENADDAARAVE 273
Query: 209 VLNFTPLNNRPIRV----MYSHRDPSIR------------KSGTANIFIKNLDKAIDHKA 340
LN +++ V S R+ +R K ++N+++KNLD +I +
Sbjct: 274 SLNGHKFDDKEWYVGRAQKKSERETELRVRYEQNLKEAADKFQSSNLYVKNLDPSISDEK 333
Query: 341 LHDTFSSFGSILSCKIATDASGQSK 415
L + FS FG++ S K+ D +G SK
Sbjct: 334 LKEIFSPFGTVTSSKVMRDPNGTSK 358
Score = 76.3 bits (186), Expect = 1e-13
Identities = 39/132 (29%), Positives = 75/132 (56%), Gaps = 4/132 (3%)
Frame = +2
Query: 32 SLYVGDLDQNVNDSQLYDLFNQVGQVVSVRVCRDLTTRRSLGYGYVNFSNPPDAARALDV 211
++++ +LD++++ L+D F+ G +VS +V D ++ +S GYG+V ++N A +A++
Sbjct: 125 NIFIKNLDESIDHKALHDTFSSFGNIVSCKVAVD-SSGQSKGYGFVQYANEESAQKAIEK 183
Query: 212 LNFTPLNNRPIRV----MYSHRDPSIRKSGTANIFIKNLDKAIDHKALHDTFSSFGSILS 379
LN LN++ + V RD + K+ N+++KNL ++ L + F +G I S
Sbjct: 184 LNGMLLNDKQVYVGPFLRRQERDSTANKTKFTNVYVKNLAESTTDDDLKNAFGEYGKITS 243
Query: 380 CKIATDASGQSK 415
+ D G+SK
Sbjct: 244 AVVMKDGEGKSK 255
Score = 21.6 bits (44), Expect(2) = 3e-54
Identities = 8/14 (57%), Positives = 10/14 (71%)
Frame = +1
Query: 403 GPVQGYGFVQFDTE 444
G +GYGFVQ+ E
Sbjct: 161 GQSKGYGFVQYANE 174
>gb|AAL86321.1| putative poly(A)-binding protein [Arabidopsis thaliana]
Length = 613
Score = 211 bits (538), Expect(2) = 3e-54
Identities = 101/137 (73%), Positives = 116/137 (83%)
Frame = +2
Query: 5 APNANFVTTSLYVGDLDQNVNDSQLYDLFNQVGQVVSVRVCRDLTTRRSLGYGYVNFSNP 184
A F TSLYVGDLD NV DSQL+D F Q+G VV+VRVCRDL TRRSLGYGYVNF+NP
Sbjct: 12 ATATQFGNTSLYVGDLDFNVTDSQLFDAFGQMGTVVTVRVCRDLVTRRSLGYGYVNFTNP 71
Query: 185 PDAARALDVLNFTPLNNRPIRVMYSHRDPSIRKSGTANIFIKNLDKAIDHKALHDTFSSF 364
DAARA+ LN+ PL +PIRVMYSHRDPS+R+SG NIFIKNLD++IDHKALHDTFSSF
Sbjct: 72 QDAARAIQELNYIPLYGKPIRVMYSHRDPSVRRSGAGNIFIKNLDESIDHKALHDTFSSF 131
Query: 365 GSILSCKIATDASGQSK 415
G+I+SCK+A D+SGQSK
Sbjct: 132 GNIVSCKVAVDSSGQSK 148
Score = 77.0 bits (188), Expect = 8e-14
Identities = 47/145 (32%), Positives = 79/145 (54%), Gaps = 16/145 (11%)
Frame = +2
Query: 29 TSLYVGDLDQNVNDSQLYDLFNQVGQVVSVRVCRDLTTRRSLGYGYVNFSNPPDAARALD 208
T++YV +L ++ D L + F + G++ S V +D +S G+G+VNF N DAARA++
Sbjct: 199 TNVYVKNLAESTTDDDLKNAFGEYGKITSAVVMKD-GEGKSKGFGFVNFENADDAARAVE 257
Query: 209 VLNFTPLNNRPIRV----MYSHRDPSIR------------KSGTANIFIKNLDKAIDHKA 340
LN +++ V S R+ +R K ++N+++KNLD +I +
Sbjct: 258 SLNGHKFDDKEWYVGRAQKKSERETELRVRYEQNLKEAADKFQSSNLYVKNLDPSISDEK 317
Query: 341 LHDTFSSFGSILSCKIATDASGQSK 415
L + FS FG++ S K+ D +G SK
Sbjct: 318 LKEIFSPFGTVTSSKVMRDPNGTSK 342
Score = 76.3 bits (186), Expect = 1e-13
Identities = 39/132 (29%), Positives = 75/132 (56%), Gaps = 4/132 (3%)
Frame = +2
Query: 32 SLYVGDLDQNVNDSQLYDLFNQVGQVVSVRVCRDLTTRRSLGYGYVNFSNPPDAARALDV 211
++++ +LD++++ L+D F+ G +VS +V D ++ +S GYG+V ++N A +A++
Sbjct: 109 NIFIKNLDESIDHKALHDTFSSFGNIVSCKVAVD-SSGQSKGYGFVQYANEESAQKAIEK 167
Query: 212 LNFTPLNNRPIRV----MYSHRDPSIRKSGTANIFIKNLDKAIDHKALHDTFSSFGSILS 379
LN LN++ + V RD + K+ N+++KNL ++ L + F +G I S
Sbjct: 168 LNGMLLNDKQVYVGPFLRRQERDSTANKTKFTNVYVKNLAESTTDDDLKNAFGEYGKITS 227
Query: 380 CKIATDASGQSK 415
+ D G+SK
Sbjct: 228 AVVMKDGEGKSK 239
Score = 21.6 bits (44), Expect(2) = 3e-54
Identities = 8/14 (57%), Positives = 10/14 (71%)
Frame = +1
Query: 403 GPVQGYGFVQFDTE 444
G +GYGFVQ+ E
Sbjct: 145 GQSKGYGFVQYANE 158
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 395,669,815
Number of Sequences: 1393205
Number of extensions: 8121247
Number of successful extensions: 26461
Number of sequences better than 10.0: 1888
Number of HSP's better than 10.0 without gapping: 23981
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25967
length of database: 448,689,247
effective HSP length: 112
effective length of database: 292,650,287
effective search space used: 11706011480
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
