KMC014323A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC014323A_C01 KMC014323A_c01
acacacaatgggtgtaagctttggcattagacctcaaagggagttacctcattagagcta
acaagtgaagttaaatgaatAGCCAAGTATATAGACACCTAATGGCTTTAAAGTTTTAAC
AGATAACGGCCGATGAAATTTGGGTAAGAGGTGCAACTTAGAGAAAAGCTAGGGGCCATG
AATATCTGGACTCCATAAAAAGGAAGTCTAGTGTGCAATGACAATTCATGGAAAAACCAT
ATCATTGAAAATTGAAGGAGATTTAATTATTCTTAAATTTGAGGCTATTACTAAAAATCT
AAAAATTGGACCTACAAATGTCAATTTCACACTAACTTGCACAAGAAACCTGTGTTGAAG
GCCTTATCTGAGGCAGCCACAGAAACCCAAAATnGGGTTAGCGAGGACCAAGTTTGGGGC
ACCCCTGGCCAAGGGGGAGGTTCAAGTTGTCCACAGTTCTAAAAGTTCTAGGTACCATCT
TATCAACATACAAAGTGCCATCCAAGTGATCACACTC


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC014323A_C01 KMC014323A_c01
         (517 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

sp|Q9FV53|DEFM_ARATH Peptide deformylase, mitochondrial precurso...    80  2e-14
ref|NP_563974.1| expressed protein; protein id: At1g15390.1, sup...    80  2e-14
sp|Q9FUZ0|DEFM_LYCES Peptide deformylase, mitochondrial precurso...    70  1e-11
ref|NP_406383.1| histidyl-tRNA synthetase [Yersinia pestis] gi|2...    33  1.9
ref|NP_668676.1| histidine tRNA synthetase [Yersinia pestis KIM]...    32  3.3

>sp|Q9FV53|DEFM_ARATH Peptide deformylase, mitochondrial precursor (PDF) (Polypeptide
           deformylase) gi|25518549|pir||E86288 hypothetical
           protein F9L1.34 - Arabidopsis thaliana
           gi|5103837|gb|AAD39667.1|AC007591_32 Simalar to
           gi|4377403 Polypeptide Deformylase from Chlamydia
           pneumoniae genome gb|AE001687. [Arabidopsis thaliana]
           gi|13605760|gb|AAK32873.1|AF361861_1 At1g15390/F9L1_34
           [Arabidopsis thaliana] gi|22136570|gb|AAM91071.1|
           At1g15390/F9L1_34 [Arabidopsis thaliana]
          Length = 259

 Score = 79.7 bits (195), Expect = 2e-14
 Identities = 34/39 (87%), Positives = 37/39 (94%)
 Frame = -1

Query: 517 ECDHLDGTLYVDKMVPRTFRTVDNLNLPLGQGCPKLGPR 401
           ECDHLDG LYVDKMVPRTFRTVDNL+LPL +GCPKLGP+
Sbjct: 221 ECDHLDGNLYVDKMVPRTFRTVDNLDLPLAEGCPKLGPQ 259

>ref|NP_563974.1| expressed protein; protein id: At1g15390.1, supported by cDNA:
           gi_11320951 [Arabidopsis thaliana]
           gi|11320952|gb|AAG33973.1|AF250959_1 peptide
           deformylase-like protein [Arabidopsis thaliana]
          Length = 269

 Score = 79.7 bits (195), Expect = 2e-14
 Identities = 34/39 (87%), Positives = 37/39 (94%)
 Frame = -1

Query: 517 ECDHLDGTLYVDKMVPRTFRTVDNLNLPLGQGCPKLGPR 401
           ECDHLDG LYVDKMVPRTFRTVDNL+LPL +GCPKLGP+
Sbjct: 231 ECDHLDGNLYVDKMVPRTFRTVDNLDLPLAEGCPKLGPQ 269

>sp|Q9FUZ0|DEFM_LYCES Peptide deformylase, mitochondrial precursor (PDF) (Polypeptide
           deformylase) gi|11320968|gb|AAG33981.1|AF271258_1
           peptide deformylase-like protein [Lycopersicon
           esculentum]
          Length = 277

 Score = 70.1 bits (170), Expect = 1e-11
 Identities = 31/37 (83%), Positives = 33/37 (88%)
 Frame = -1

Query: 517 ECDHLDGTLYVDKMVPRTFRTVDNLNLPLGQGCPKLG 407
           E DHLDGTLYVDKM PRTFRTV+NL+LPL  GCPKLG
Sbjct: 239 EYDHLDGTLYVDKMAPRTFRTVENLDLPLAAGCPKLG 275

>ref|NP_406383.1| histidyl-tRNA synthetase [Yersinia pestis] gi|25293367|pir||AF0350
           histidine-tRNA ligase (EC 6.1.1.21) [imported] -
           Yersinia pestis (strain CO92)
           gi|15980845|emb|CAC92129.1| histidyl-tRNA synthetase
           [Yersinia pestis CO92]
          Length = 424

 Score = 33.1 bits (74), Expect = 1.9
 Identities = 12/31 (38%), Positives = 20/31 (63%)
 Frame = -2

Query: 387 GFCGCLR*GLQHRFLVQVSVKLTFVGPIFRF 295
           G  GC+R G++H  L     +L ++GP+FR+
Sbjct: 84  GTAGCVRAGIEHGLLYNQEQRLWYIGPMFRY 114

>ref|NP_668676.1| histidine tRNA synthetase [Yersinia pestis KIM]
           gi|21958124|gb|AAM84927.1|AE013738_4 histidine tRNA
           synthetase [Yersinia pestis KIM]
          Length = 424

 Score = 32.3 bits (72), Expect = 3.3
 Identities = 12/31 (38%), Positives = 19/31 (60%)
 Frame = -2

Query: 387 GFCGCLR*GLQHRFLVQVSVKLTFVGPIFRF 295
           G  GC R G++H  L     +L ++GP+FR+
Sbjct: 84  GTAGCARAGIEHGLLYNQEQRLWYIGPMFRY 114

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 432,520,211
Number of Sequences: 1393205
Number of extensions: 8735012
Number of successful extensions: 24093
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 23258
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 24090
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 16154357632
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 SPD030b04_f BP046354 1 517
2 MWL003e04_f AV768629 243 368




Lotus japonicus
Kazusa DNA Research Institute