KMC013781A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC013781A_C01 KMC013781A_c01
ataaAGAGAAAGAAGGAGGCGTATATTAAGTATCTGATCTTGATGCATTACTAATGGTAT
TAACAAATGAAAGCTAGGTATAAACAGCAGTGGAAGCTATAGCCCCTTGGCCCAAACAAA
CTATGCCAAACTATGCCTGCCTAGTCCCTACCTATCATAGTTACATAATATAGTTTCTTT
ACAGAGAGTAATGTCAGTGGGTAATGGCTATAAGAATCTGATGACGATTTTATAATTCTC
ATCATCTCTTGAAGCCTCTTAAGAAATTCTTCTACAATTGATTCTGAAGTAAGAACCAAT
TCTAGGAAAAACTTCTTAGTAGCTTTTGAGTTTnGGCATTGATTCTGATAAAAGAGAAAC
CAATCTACGTATGCTATAAGACTAACATATCCTGAAATTGGAGAGATCCAAGTGTCTGAA
CCAGCCTTAGAAACAGAGAAATGAAGTAAACAACTAGAAGCCCTCCTCCAAGGACTGCAT
CAAGCTTCATATCCCTCCTAATCAGAACCACAAGTGCCCAAACCAATCCAGCCACCAAAA


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC013781A_C01 KMC013781A_c01
         (540 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_197287.1| potassium-dependent sodium-calcium exchanger - ...    52  4e-06
ref|NP_197288.1| potassium-dependent sodium-calcium exchanger - ...    50  1e-05
gb|AAL58918.1|AF462830_1 AT5g17860/MVA3_210 [Arabidopsis thaliana]     50  1e-05
gb|AAN65432.1| Putative potassium-dependent sodium-calcium excha...    45  4e-04
ref|NP_566474.1| similar to potassium-dependent sodium-calcium e...    44  0.002

>ref|NP_197287.1| potassium-dependent sodium-calcium exchanger - like protein;
           protein id: At5g17850.1 [Arabidopsis thaliana]
          Length = 559

 Score = 52.4 bits (124), Expect = 4e-06
 Identities = 25/44 (56%), Positives = 33/44 (74%)
 Frame = -3

Query: 538 LVAGLVWALVVLIRRDMKLDAVLGGGLLVVYFISLFLRLVQTLG 407
           LVAGLVW+ +VL    M+L  V+G GLLV+Y  SL LR++QT+G
Sbjct: 513 LVAGLVWSFLVLFSNRMRLGGVMGIGLLVIYLASLSLRIMQTVG 556

>ref|NP_197288.1| potassium-dependent sodium-calcium exchanger - like protein;
           protein id: At5g17860.1, supported by cDNA: gi_18087574
           [Arabidopsis thaliana] gi|10177887|dbj|BAB11219.1|
           contains similarity to unknown
           protein~gb|AAD25785.1~gene_id:MPI7.2 [Arabidopsis
           thaliana] gi|22655386|gb|AAM98285.1| At5g17860/MVA3_210
           [Arabidopsis thaliana]
          Length = 570

 Score = 50.4 bits (119), Expect = 1e-05
 Identities = 22/46 (47%), Positives = 33/46 (70%)
 Frame = -3

Query: 538 LVAGLVWALVVLIRRDMKLDAVLGGGLLVVYFISLFLRLVQTLGSL 401
           L+ GL+WALV++ ++ M+LD ++GGGLL +Y   L LRL +  G L
Sbjct: 521 LMVGLLWALVIMPKKKMRLDKLVGGGLLAIYLCFLSLRLARVFGVL 566

>gb|AAL58918.1|AF462830_1 AT5g17860/MVA3_210 [Arabidopsis thaliana]
          Length = 570

 Score = 50.4 bits (119), Expect = 1e-05
 Identities = 22/46 (47%), Positives = 33/46 (70%)
 Frame = -3

Query: 538 LVAGLVWALVVLIRRDMKLDAVLGGGLLVVYFISLFLRLVQTLGSL 401
           L+ GL+WALV++ ++ M+LD ++GGGLL +Y   L LRL +  G L
Sbjct: 521 LMVGLLWALVIMPKKKMRLDKLVGGGLLAIYLCFLSLRLARVFGVL 566

>gb|AAN65432.1| Putative potassium-dependent sodium-calcium exchanger-like protein
           [Oryza sativa (japonica cultivar-group)]
           gi|27436756|gb|AAO13475.1| Putative potassium-dependent
           sodium-calcium exchanger-like protein [Oryza sativa
           (japonica cultivar-group)]
          Length = 639

 Score = 45.4 bits (106), Expect = 4e-04
 Identities = 21/46 (45%), Positives = 32/46 (68%)
 Frame = -3

Query: 538 LVAGLVWALVVLIRRDMKLDAVLGGGLLVVYFISLFLRLVQTLGSL 401
           LV GL+WALV+L R  M+ + +LG GL+ +Y + LF+R+   +G L
Sbjct: 588 LVGGLIWALVMLPRGGMQPNKILGVGLIALYSVFLFIRVSNAMGIL 633

>ref|NP_566474.1| similar to potassium-dependent sodium-calcium exchanger - like
           protein; protein id: At3g14070.1 [Arabidopsis thaliana]
           gi|9294631|dbj|BAB02970.1| Na/Ca,K-exchanger-like
           protein [Arabidopsis thaliana]
          Length = 643

 Score = 43.5 bits (101), Expect = 0.002
 Identities = 21/44 (47%), Positives = 28/44 (62%)
 Frame = -3

Query: 538 LVAGLVWALVVLIRRDMKLDAVLGGGLLVVYFISLFLRLVQTLG 407
           LV GLVWA+V+L R DM+    LG GL+ +Y I +  RL   +G
Sbjct: 594 LVLGLVWAMVILPRNDMQPSRTLGVGLIAIYLIFVTFRLSCAMG 637

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 414,123,419
Number of Sequences: 1393205
Number of extensions: 7830536
Number of successful extensions: 16199
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 15826
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 16165
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 18462123008
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 MPDL062a09_f AV779636 1 227
2 SPDL081e07_f BP057061 5 540




Lotus japonicus
Kazusa DNA Research Institute