Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC013578A_C01 KMC013578A_c01
(574 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||S71467 diacylglycerol kinase (EC 2.7.1.107) ATDGK1 - Arabid... 86 3e-16
ref|NP_196409.1| diacylglycerol kinase (ATDGK1); protein id: At5... 86 3e-16
ref|NP_201182.1| diacylglycerol kinase; protein id: At5g63770.1,... 62 7e-09
ref|XP_228055.1| similar to testis expressed sequence 27; likely... 35 0.51
sp|P34125|KMHC_DICDI Myosin heavy chain kinase (MHCK) gi|423914|... 32 4.3
>pir||S71467 diacylglycerol kinase (EC 2.7.1.107) ATDGK1 - Arabidopsis thaliana
gi|1374772|dbj|BAA09856.1| diacylglycerol kinase
[Arabidopsis thaliana]
Length = 728
Score = 86.3 bits (212), Expect = 3e-16
Identities = 44/49 (89%), Positives = 46/49 (93%)
Frame = -2
Query: 354 AFMLKRAAEEPLGHAAAIITDVLENAETNNVINATQKRALLHEMALRLS 208
AFMLKRAAEEPLGHAAAIITDVLENAETN VINA+QKR LL EMALRL+
Sbjct: 680 AFMLKRAAEEPLGHAAAIITDVLENAETNQVINASQKRTLLQEMALRLT 728
>ref|NP_196409.1| diacylglycerol kinase (ATDGK1); protein id: At5g07920.1
[Arabidopsis thaliana] gi|20141593|sp|Q39017|KDG1_ARATH
Diacylglycerol kinase 1 (Diglyceride kinase 1) (DGK 1)
(DAG kinase 1) gi|6562306|emb|CAB62604.1| diacylglycerol
kinase (ATDGK1) [Arabidopsis thaliana]
gi|10176726|dbj|BAB09956.1| diacylglycerol kinase ATDGK1
homolog [Arabidopsis thaliana]
gi|28393496|gb|AAO42169.1| putative diacylglycerol
kinase (ATDGK1) [Arabidopsis thaliana]
Length = 728
Score = 86.3 bits (212), Expect = 3e-16
Identities = 44/49 (89%), Positives = 46/49 (93%)
Frame = -2
Query: 354 AFMLKRAAEEPLGHAAAIITDVLENAETNNVINATQKRALLHEMALRLS 208
AFMLKRAAEEPLGHAAAIITDVLENAETN VINA+QKR LL EMALRL+
Sbjct: 680 AFMLKRAAEEPLGHAAAIITDVLENAETNQVINASQKRTLLQEMALRLT 728
>ref|NP_201182.1| diacylglycerol kinase; protein id: At5g63770.1, supported by cDNA:
gi_17065157 [Arabidopsis thaliana]
gi|10177058|dbj|BAB10470.1| diacylglycerol kinase
[Arabidopsis thaliana] gi|17065158|gb|AAL32733.1|
diacylglycerol kinase [Arabidopsis thaliana]
Length = 712
Score = 61.6 bits (148), Expect = 7e-09
Identities = 30/48 (62%), Positives = 39/48 (80%)
Frame = -2
Query: 351 FMLKRAAEEPLGHAAAIITDVLENAETNNVINATQKRALLHEMALRLS 208
FML+RA++EP GHAAAI+ +VL +AE VINA+QK+ LL +MAL LS
Sbjct: 665 FMLRRASDEPRGHAAAIMNEVLLDAECKGVINASQKKVLLQQMALHLS 712
>ref|XP_228055.1| similar to testis expressed sequence 27; likely ortholog of mouse
testis expressed gene 27 [Homo sapiens] [Rattus
norvegicus]
Length = 336
Score = 35.4 bits (80), Expect = 0.51
Identities = 22/75 (29%), Positives = 32/75 (42%)
Frame = +1
Query: 142 VCKVYSQKTGTKTLITGWRIFLGQPQCHLMKKCSLLCSINYIIGFSILKHISDYCSCMTK 321
+C +S+ T L TG Q QCH + L S+ +G+ + + Y CM
Sbjct: 240 LCVTFSRVANTHFLCTG------QGQCHTFPRVELQISLYQEVGWELPARVRGYVFCMLH 293
Query: 322 RFLSRPLQHEGLNDH 366
R P QH+ DH
Sbjct: 294 RL---PEQHDCTFDH 305
>sp|P34125|KMHC_DICDI Myosin heavy chain kinase (MHCK) gi|423914|pir||A46136
myosin-heavy-chain kinase (EC 2.7.1.129) - slime mold
(Dictyostelium discoideum)
Length = 783
Score = 32.3 bits (72), Expect = 4.3
Identities = 17/46 (36%), Positives = 26/46 (55%)
Frame = +2
Query: 392 FLMHLMVRSITKKTKI*GHIKSET*YKDETNS*SMTEHFHHKNHGN 529
F++HL+++S TK I S T T++ + T HF+H NH N
Sbjct: 738 FILHLLLKSTTKFKSI-STTTSTTSITTSTSTSTSTTHFNHPNHQN 782
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 460,616,031
Number of Sequences: 1393205
Number of extensions: 9246309
Number of successful extensions: 22003
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 21435
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 21994
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 21243732558
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
