>KMC013228A_C01 KMC013228A_c01
gcaaagcaaaatgaatctctatggatctgacgaagtgggtaatACACGAATGATTGGGCC
GGATCGGGTTTTGTTCACCCAATACCCACCAGTGTATTAACAATCCCAGCCATAAAGCGT
AATTAAACTGCCACAACTGAGAAGATTCCAGATATGAATATCCAACTTACTTAACTATTG
TACAAAAATCTCTAATAAACATGGCAAAAACTTTTATGTTTTGTTACTACAACACTGCAA
TTAGAACGTAATGCTTGACCATTGACACCATCATAATATTCAGTATTTTGATCACTGGAG
AGAAATCTCATGCTAATGTTATTCAACTAATACCATTGCACATTTAACCAAGGAAGACCA
TATCAACATGTGAATCCAATGTGTTGGTACTCCTTTTGTCATCAAGCAATGTCTGTCTAC
CCGGCTTCTGCAACCTGGTTCCAGCACCATGCATCATTAAACAGGAGAATCTAGTCCCAG
TACTTTATCAATCCATCCCAGCCGCATGTGGCAACCTGGCTCTGTTCAAGGGGATGCCAT
TCACAACCAATGCAAACACCTTCGTGACACTTGAGAGTTCTGAAGACTTGGCAACTCTTC
CAATCCCAGAACCAACACTTACCATCACCATCACCTGACATGACAAAGCGTCCATCGGGC
GAAAAATTGACCTG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC013228A_C01 KMC013228A_c01
         (674 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_172528.1| putative pre-mRNA splicing factor; protein id: ...   150  2e-35
ref|NP_651005.1| CG6015-PA [Drosophila melanogaster] gi|7300806|...   100  1e-20
ref|NP_056975.1| pre-mRNA splicing factor 17; EH-binding protein...    98  9e-20
sp|Q9DC48|PR17_MOUSE Pre-mRNA splicing factor PRP17 gi|12835829|...    98  9e-20
gb|AAC25166.1| splicing factor hPRP17 [Homo sapiens]                   98  9e-20

>ref|NP_172528.1| putative pre-mRNA splicing factor; protein id: At1g10580.1
           [Arabidopsis thaliana] gi|25402606|pir||C86239 protein
           T10O24.21 [imported] - Arabidopsis thaliana
           gi|5091552|gb|AAD39581.1|AC007067_21 T10O24.21
           [Arabidopsis thaliana]
          Length = 616

 Score =  150 bits (379), Expect = 2e-35
 Identities = 61/66 (92%), Positives = 64/66 (96%)
 Frame = -1

Query: 674 QVNFSPDGRFVMSGDGDGKCWFWDWKSCQVFRTLKCHEGVCIGCEWHPLEQSQVATCGWD 495
           QVNFSPDGRFVMSGDG+GKCWFWDWKSC+VFRTLKCH GVCIG EWHPLEQS+VATCGWD
Sbjct: 507 QVNFSPDGRFVMSGDGEGKCWFWDWKSCKVFRTLKCHNGVCIGAEWHPLEQSKVATCGWD 566

Query: 494 GLIKYW 477
           GLIKYW
Sbjct: 567 GLIKYW 572

>ref|NP_651005.1| CG6015-PA [Drosophila melanogaster] gi|7300806|gb|AAF55949.1|
           CG6015-PA [Drosophila melanogaster]
           gi|17862316|gb|AAL39635.1| LD21931p [Drosophila
           melanogaster]
          Length = 576

 Score =  100 bits (250), Expect = 1e-20
 Identities = 38/67 (56%), Positives = 52/67 (76%)
 Frame = -1

Query: 674 QVNFSPDGRFVMSGDGDGKCWFWDWKSCQVFRTLKCHEGVCIGCEWHPLEQSQVATCGWD 495
           Q++FSPD  +++SGDGDGKC+ WDWK+ ++++  + H+GVCI   WHP E S+V T GWD
Sbjct: 510 QLDFSPDMSYLVSGDGDGKCYIWDWKTTKMYKKWQAHDGVCISALWHPHEASKVVTAGWD 569

Query: 494 GLIKYWD 474
           G IKYWD
Sbjct: 570 GQIKYWD 576

>ref|NP_056975.1| pre-mRNA splicing factor 17; EH-binding protein 3 [Homo sapiens]
           gi|17380181|sp|O60508|PR17_HUMAN Pre-mRNA splicing
           factor PRP17 (hPRP17) (EH-binding protein 3) (Ehb3)
           gi|3123908|gb|AAC39730.1| pre-mRNA splicing factor [Homo
           sapiens]
          Length = 579

 Score = 98.2 bits (243), Expect = 9e-20
 Identities = 40/67 (59%), Positives = 50/67 (73%)
 Frame = -1

Query: 674 QVNFSPDGRFVMSGDGDGKCWFWDWKSCQVFRTLKCHEGVCIGCEWHPLEQSQVATCGWD 495
           QV+FSPD  +V+SGDG+GK   WDWK+ +++   K H+ VCIG  WHP E S+V TCGWD
Sbjct: 513 QVDFSPDMSYVISGDGNGKLNIWDWKTTKLYSRFKAHDKVCIGAVWHPHETSKVITCGWD 572

Query: 494 GLIKYWD 474
           GLIK WD
Sbjct: 573 GLIKLWD 579

>sp|Q9DC48|PR17_MOUSE Pre-mRNA splicing factor PRP17 gi|12835829|dbj|BAB23380.1| unnamed
           protein product [Mus musculus]
          Length = 579

 Score = 98.2 bits (243), Expect = 9e-20
 Identities = 40/67 (59%), Positives = 50/67 (73%)
 Frame = -1

Query: 674 QVNFSPDGRFVMSGDGDGKCWFWDWKSCQVFRTLKCHEGVCIGCEWHPLEQSQVATCGWD 495
           QV+FSPD  +V+SGDG+GK   WDWK+ +++   K H+ VCIG  WHP E S+V TCGWD
Sbjct: 513 QVDFSPDMSYVISGDGNGKLNIWDWKTTKLYSRFKAHDKVCIGAVWHPHETSKVITCGWD 572

Query: 494 GLIKYWD 474
           GLIK WD
Sbjct: 573 GLIKLWD 579

>gb|AAC25166.1| splicing factor hPRP17 [Homo sapiens]
          Length = 542

 Score = 98.2 bits (243), Expect = 9e-20
 Identities = 40/67 (59%), Positives = 50/67 (73%)
 Frame = -1

Query: 674 QVNFSPDGRFVMSGDGDGKCWFWDWKSCQVFRTLKCHEGVCIGCEWHPLEQSQVATCGWD 495
           QV+FSPD  +V+SGDG+GK   WDWK+ +++   K H+ VCIG  WHP E S+V TCGWD
Sbjct: 476 QVDFSPDMSYVISGDGNGKLNIWDWKTTKLYSRFKAHDKVCIGAVWHPHETSKVITCGWD 535

Query: 494 GLIKYWD 474
           GLIK WD
Sbjct: 536 GLIKLWD 542

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 620,919,929
Number of Sequences: 1393205
Number of extensions: 13880131
Number of successful extensions: 43289
Number of sequences better than 10.0: 743
Number of HSP's better than 10.0 without gapping: 38835
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 42893
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 29704274460
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 SPD083f05_f BP050644 1 234 2 MPDL026h07_f AV777825 44 425 3 SPD036e09_f BP046875 156 676

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