>KMC013144A_C01 KMC013144A_c01
cctccaacattcattcataaactgttccattcaccacattTCGGATTCAAAACATTCATC
ATCATCATCATCATCATCATCATCATCATCTCTGTCACCAATCCACACCATGTCATCGAA
TCCAGAAATGGGAGCAGACGACGACGTTGAAGCCGCCGGCGCAGACGGCGGTTTCCGCTC
CCCGATCGGCCGCAAGTACCGCCCTGTCTTAGCCAACGATCGTGCCGTCCTCGAAATGTC
TTCCATAGATCCAGGATCTTCCTCTTCGTCTTCCTCTGTCATTCCAGATCCCCCTCCTAA
TCTCAGGAAAATCAATGTAGGTAGCAGTTTGAATGCGAGTTCAGATGCTAAAGAAGGGAA
ATCTTCTCATCCACCACAACCTAATGGTCCCCAGCAGGATTCTAAGCTTGAATTGTTTGG
TTTTGATTCTCTTGTCAACATTCTTGGTCTTAAAAGCATGACTGGTGAGCAAGTTGCAGC
ACCATCTAGTCCTAGAGATGGTGAGGACATTACCATCCCTGCTGGGCTACCAAAGCCTCC
TGCTCTCAGACTAGGT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC013144A_C01 KMC013144A_c01
         (556 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_174333.1| cation-chloride co-transporter, putative; prote...   140  1e-32
pir||H86428 hypothetical protein F26G16.3 [imported] - Arabidops...   140  1e-32
pir||T01896 probable Na+/K+/Cl-cotransport protein - common toba...   137  1e-31
dbj|BAB20646.1| putative cation-chloride co-transporter [Oryza s...    84  2e-15
ref|NP_081602.2| RIKEN cDNA 2210011H10; DNA segment, Chr 4, ERAT...    49  5e-05

>ref|NP_174333.1| cation-chloride co-transporter, putative; protein id: At1g30450.1
           [Arabidopsis thaliana]
          Length = 1321

 Score =  140 bits (352), Expect = 1e-32
 Identities = 83/143 (58%), Positives = 98/143 (68%), Gaps = 5/143 (3%)
 Frame = +2

Query: 143 DVEAAGADGG--FRSPI---GRKYRPVLANDRAVLEMSSIDPGSSSSSSSVIPDPPPNLR 307
           D+E AG +G   FRS     G KYRPV+A+DRAV+EMSSIDPGSSSS+   I    P   
Sbjct: 5   DIEEAGGNGEEEFRSGPRLGGSKYRPVVAHDRAVVEMSSIDPGSSSSTLKNIKVVAPG-- 62

Query: 308 KINVGSSLNASSDAKEGKSSHPPQPNGPQQDSKLELFGFDSLVNILGLKSMTGEQVAAPS 487
             +VG+ +    D            NG Q++SKLELFGFDSLVNILGLKSMTGEQ+ APS
Sbjct: 63  --DVGAGVRGPEDGV----------NGHQKESKLELFGFDSLVNILGLKSMTGEQIQAPS 110

Query: 488 SPRDGEDITIPAGLPKPPALRLG 556
           SPRDGEDI+I  G PKPPAL++G
Sbjct: 111 SPRDGEDISITQGHPKPPALKMG 133

>pir||H86428 hypothetical protein F26G16.3 [imported] - Arabidopsis thaliana
           gi|6634764|gb|AAF19744.1|AC009917_3 Strong similarity to
           gb|AF021220 Nicotiana tabacum cation-chloride
           co-transporter and contains an amino acid permease
           PF|00324 domain. [Arabidopsis thaliana]
          Length = 973

 Score =  140 bits (352), Expect = 1e-32
 Identities = 83/143 (58%), Positives = 98/143 (68%), Gaps = 5/143 (3%)
 Frame = +2

Query: 143 DVEAAGADGG--FRSPI---GRKYRPVLANDRAVLEMSSIDPGSSSSSSSVIPDPPPNLR 307
           D+E AG +G   FRS     G KYRPV+A+DRAV+EMSSIDPGSSSS+   I    P   
Sbjct: 5   DIEEAGGNGEEEFRSGPRLGGSKYRPVVAHDRAVVEMSSIDPGSSSSTLKNIKVVAPG-- 62

Query: 308 KINVGSSLNASSDAKEGKSSHPPQPNGPQQDSKLELFGFDSLVNILGLKSMTGEQVAAPS 487
             +VG+ +    D            NG Q++SKLELFGFDSLVNILGLKSMTGEQ+ APS
Sbjct: 63  --DVGAGVRGPEDGV----------NGHQKESKLELFGFDSLVNILGLKSMTGEQIQAPS 110

Query: 488 SPRDGEDITIPAGLPKPPALRLG 556
           SPRDGEDI+I  G PKPPAL++G
Sbjct: 111 SPRDGEDISITQGHPKPPALKMG 133

>pir||T01896 probable Na+/K+/Cl-cotransport protein - common tobacco
           gi|2582381|gb|AAC49874.1| cation-chloride co-transporter
           [Nicotiana tabacum]
          Length = 990

 Score =  137 bits (344), Expect = 1e-31
 Identities = 79/155 (50%), Positives = 103/155 (65%), Gaps = 3/155 (1%)
 Frame = +2

Query: 101 IHTMSSNPEMGADDDVEAAGADGGFRSPIGRKYRPVLA---NDRAVLEMSSIDPGSSSSS 271
           +H+  +   +   +D+E A     F + +GRKY PV+A   ND AV+EM+SI PGSSSS 
Sbjct: 1   MHSDQNKEAIDDGEDIEIADDINQFPTGVGRKYSPVVAHDVNDSAVVEMTSIHPGSSSSF 60

Query: 272 SSVIPDPPPNLRKINVGSSLNASSDAKEGKSSHPPQPNGPQQDSKLELFGFDSLVNILGL 451
                 P   L+K+ VG   N +S+ +E +S+     NGPQ++SKLELFGFDSLVNILGL
Sbjct: 61  ------PKHELKKVKVGVQPNMASEERE-ESAANHNINGPQRESKLELFGFDSLVNILGL 113

Query: 452 KSMTGEQVAAPSSPRDGEDITIPAGLPKPPALRLG 556
           KSMTG+Q+ APSSPRDGED+TI    PKP A + G
Sbjct: 114 KSMTGDQIQAPSSPRDGEDVTITFEQPKPTADKSG 148

>dbj|BAB20646.1| putative cation-chloride co-transporter [Oryza sativa (japonica
           cultivar-group)]
          Length = 1270

 Score = 83.6 bits (205), Expect = 2e-15
 Identities = 69/185 (37%), Positives = 92/185 (49%), Gaps = 44/185 (23%)
 Frame = +2

Query: 131 GADDDVEAAGADGGFR----SPIGR-----KYRPVLANDRAVLEMSSIDPGSSSSSSS-- 277
           G   D+E  G  G  R    +P  R     +YR V ++DRAV++M+ ++ GSS+ +S+  
Sbjct: 198 GGGGDMERGGFGGAGRHDEEAPAMRPAPQQRYRTVESHDRAVVQMAPMEFGSSADASASA 257

Query: 278 ---VIP-----------------------------DPPPNLRKINVGSSLNASSDAK-EG 358
              +IP                                 NLR I  G++L   +DA+   
Sbjct: 258 GPRMIPIAKSLRVLVVRIRLGVFYWLDRWSNAPTLSHSSNLRYIKPGTNLR--TDARMHM 315

Query: 359 KSSHPPQPNGPQQDSKLELFGFDSLVNILGLKSMTGEQVAAPSSPRDGEDITIPAGLPKP 538
            SS+    NG Q DSKLELFGFDSLVNILGLK M GEQ  A +S RDGE+  I  G PK 
Sbjct: 316 ASSNGRSSNGSQGDSKLELFGFDSLVNILGLKRMVGEQAQASASTRDGENAGIAIGHPKL 375

Query: 539 PALRL 553
            ++ L
Sbjct: 376 SSIPL 380

>ref|NP_081602.2| RIKEN cDNA 2210011H10; DNA segment, Chr 4, ERATO Doi 321, expressed
           [Mus musculus] gi|18203961|gb|AAH21420.1| Similar to
           myeloid/lymphoid or mixed-lineage leukemia (trithorax
           homolog, Drosophila); translocated to, 3 [Mus musculus]
          Length = 557

 Score = 48.5 bits (114), Expect = 5e-05
 Identities = 41/117 (35%), Positives = 55/117 (46%), Gaps = 9/117 (7%)
 Frame = +2

Query: 41  SDSKHSS-SSSSSSSSSSLSPIHTMSSNP------EMGADDDVEAA--GADGGFRSPIGR 193
           SDS+  S +SSSSSSSSS +P  T    P      ++ +DD+ E +    D    S + R
Sbjct: 363 SDSEQPSPASSSSSSSSSFTPSQTRQQGPLRSIMKDLHSDDNEEESDEAEDNDNDSEMER 422

Query: 194 KYRPVLANDRAVLEMSSIDPGSSSSSSSVIPDPPPNLRKINVGSSLNASSDAKEGKS 364
                 +  R V      D  SSS+SS +  +PPP L K N    L   S  K+ KS
Sbjct: 423 PVNRGGSRSRRVSLSDGSDSESSSASSPLHHEPPPPLLKTNNNQILEVKSPIKQSKS 479

 Score = 34.3 bits (77), Expect = 1.0
 Identities = 21/34 (61%), Positives = 23/34 (66%)
 Frame = +2

Query: 20  NCSIHHISDSKHSSSSSSSSSSSSLSPIHTMSSN 121
           N SIH  S S  SSSSSSSSSSSS S   + SS+
Sbjct: 143 NRSIHTSSSSSSSSSSSSSSSSSSSSSSSSSSSS 176

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 521,876,967
Number of Sequences: 1393205
Number of extensions: 12803287
Number of successful extensions: 231819
Number of sequences better than 10.0: 2568
Number of HSP's better than 10.0 without gapping: 77265
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 149581
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 19521267756
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MFL013h02_f BP033785 1 556 2 MPDL020e01_f AV777512 41 464

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