>KMC012995A_C01 KMC012995A_c01
gaaCGTTTAAAAATAATATTTCTTTTTAGGATGGTTTAACAATCTCAGCTTCCCTTTTTA
TTGCACTGATTGAAAAAACAATGGAAATTGACATGGTCGATACGTCTCATCCGTGACATA
CAATTGGAAATATGAATTCTCTTGAAGACATAGTATGAAAACAAAAAATAAACTCATGCA
TTTCTTTTTATCAGTTCACAAGTTTTCCTGTGACATCTCTAAAGAGCTTCATGGATGGCT
TTTCCATGGTAATTCCAGCCATGAAGGGTAACTCGAGGAGCGACACCTGGAGGGACCTCG
AAAGAGATTTTAACAGTCATGGCCTCTCCGGGCACCAGTGAAAAATAGTTATCTGAGTAG
TGAACAGGGAGAATTCTTGTGTCTTCCCCTTCCTTGTGGTCCCTATTTGAAGCATGAACA
GAAAAATGAAGAAAGAAAGCAACTCCTATATCTTGTCCATTTATTTCAGAAACCTTCAAG
CCATCACTTTTCCCAGCTAAGCATCTGTGTATCCTCTTAAAncaancaanttc

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC012995A_C01 KMC012995A_c01
         (533 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAK84953.2| putative beta-mannosidase [Gossypium hirsutum]         148  5e-35
gb|AAO27794.1| glycosyl hydrolase [Gossypium hirsutum]                148  5e-35
ref|NP_563833.1| expressed protein; protein id: At1g09010.1, sup...   105  3e-22
pir||A86222 hypothetical protein [imported] - Arabidopsis thalia...    82  4e-15
ref|NP_638508.1| glycosyl hydrolase [Xanthomonas campestris pv. ...    47  1e-04

>gb|AAK84953.2| putative beta-mannosidase [Gossypium hirsutum]
          Length = 786

 Score =  148 bits (373), Expect = 5e-35
 Identities = 68/100 (68%), Positives = 85/100 (85%)
 Frame = -1

Query: 521 FKRIHRCLAGKSDGLKVSEINGQDIGVAFFLHFSVHASNRDHKEGEDTRILPVHYSDNYF 342
           F+R++R  + +SDGL+V+EING D GVAFFL+FSVH +  +H+EGED+RILPVHYSDNYF
Sbjct: 688 FQRLYRQFSRESDGLRVAEINGSDGGVAFFLNFSVHGAKLEHEEGEDSRILPVHYSDNYF 747

Query: 341 SLVPGEAMTVKISFEVPPGVAPRVTLHGWNYHGKAIHEAL 222
           SLVPGE M++KISF+VPPGV+PRVTL GWNYH   +H  L
Sbjct: 748 SLVPGEEMSIKISFKVPPGVSPRVTLRGWNYH-HGVHTVL 786

>gb|AAO27794.1| glycosyl hydrolase [Gossypium hirsutum]
          Length = 976

 Score =  148 bits (373), Expect = 5e-35
 Identities = 68/100 (68%), Positives = 85/100 (85%)
 Frame = -1

Query: 521  FKRIHRCLAGKSDGLKVSEINGQDIGVAFFLHFSVHASNRDHKEGEDTRILPVHYSDNYF 342
            F+R++R  + +SDGL+V+EING D GVAFFL+FSVH +  +H+EGED+RILPVHYSDNYF
Sbjct: 878  FQRLYRQFSRESDGLRVAEINGSDGGVAFFLNFSVHGAKLEHEEGEDSRILPVHYSDNYF 937

Query: 341  SLVPGEAMTVKISFEVPPGVAPRVTLHGWNYHGKAIHEAL 222
            SLVPGE M++KISF+VPPGV+PRVTL GWNYH   +H  L
Sbjct: 938  SLVPGEEMSIKISFKVPPGVSPRVTLRGWNYH-HGVHTVL 976

>ref|NP_563833.1| expressed protein; protein id: At1g09010.1, supported by cDNA:
           gi_15028222, supported by cDNA: gi_20259144 [Arabidopsis
           thaliana]
          Length = 647

 Score =  105 bits (263), Expect = 3e-22
 Identities = 57/94 (60%), Positives = 66/94 (69%), Gaps = 4/94 (4%)
 Frame = -1

Query: 518 KRIHRCL--AGKSDGLKVSEINGQDIGVAFFLHFSVHASNRDHKEGEDTRILPVHYSDNY 345
           K   RC+  A  + GLKV E+ G D GVAFFL FSVH +     E +DTRILPVHYSDNY
Sbjct: 549 KLFSRCVVSADSNRGLKVVEMKGSDSGVAFFLRFSVHNAET---EKQDTRILPVHYSDNY 605

Query: 344 FSLVPGEAMTVKISFEVPPGV--APRVTLHGWNY 249
           FSLVPGE+M+ KISF  P G+  +PRV L GWNY
Sbjct: 606 FSLVPGESMSFKISFAAPTGMKKSPRVMLQGWNY 639

>pir||A86222 hypothetical protein [imported] - Arabidopsis thaliana
           gi|2342683|gb|AAB70407.1| Contains similarity to Bos
           beta-mannosidase (gb|U46067). [Arabidopsis thaliana]
          Length = 810

 Score = 82.0 bits (201), Expect = 4e-15
 Identities = 45/78 (57%), Positives = 49/78 (62%)
 Frame = -1

Query: 482 GLKVSEINGQDIGVAFFLHFSVHASNRDHKEGEDTRILPVHYSDNYFSLVPGEAMTVKIS 303
           GLKV E+ G D GVAFFL FSVH +     E +DTRILPVHYSDNYFSLVPG        
Sbjct: 740 GLKVVEMKGSDSGVAFFLRFSVHNAET---EKQDTRILPVHYSDNYFSLVPGMKK----- 791

Query: 302 FEVPPGVAPRVTLHGWNY 249
                  +PRV L GWNY
Sbjct: 792 -------SPRVMLQGWNY 802

>ref|NP_638508.1| glycosyl hydrolase [Xanthomonas campestris pv. campestris str. ATCC
           33913] gi|21114390|gb|AAM42432.1| glycosyl hydrolase
           [Xanthomonas campestris pv. campestris str. ATCC 33913]
          Length = 890

 Score = 47.4 bits (111), Expect = 1e-04
 Identities = 23/48 (47%), Positives = 30/48 (61%), Gaps = 1/48 (2%)
 Frame = -1

Query: 392 EGEDTRILPVHYSDNYFSLVPGEAMTVKISFEVPPGV-APRVTLHGWN 252
           + +  RILP +YSDNY +L PGE+ TV+I       + A RV L GWN
Sbjct: 831 DAQGQRILPAYYSDNYLNLAPGESRTVEIRGPAEASLRAARVQLRGWN 878

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 444,152,574
Number of Sequences: 1393205
Number of extensions: 9395984
Number of successful extensions: 21758
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 21174
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 21751
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 17885181664
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 SPD016d09_f BP045260 1 422 2 MPDL009e02_f AV776979 4 374 3 MFL021e12_f BP033893 28 520 4 SPD055b01_f BP048359 33 441 5 SPDL028h01_f BP053761 74 538

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