Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC012981A_C01 KMC012981A_c01
(1109 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||T03964 probable ubiquitin-protein ligase (EC 6.3.2.19) - co... 669 0.0
ref|NP_565693.1| ubiquitin activating enzyme 1 (UBA1); protein i... 661 0.0
gb|AAL90910.1| At2g30110/T27E13.15 [Arabidopsis thaliana] 661 0.0
ref|NP_568168.1| ubiquitin activating enzyme 2 (UBA2); protein i... 658 0.0
sp|P20973|UBA1_WHEAT Ubiquitin-activating enzyme E1 1 gi|100841|... 634 0.0
>pir||T03964 probable ubiquitin-protein ligase (EC 6.3.2.19) - common tobacco
gi|1808656|emb|CAA71762.1| Ubiquitin activating enzyme E1
[Nicotiana tabacum]
Length = 1080
Score = 669 bits (1727), Expect = 0.0
Identities = 318/366 (86%), Positives = 346/366 (93%)
Frame = +3
Query: 12 DGRLEDVNPQLLRQFAFGARAVLNPMAAMFGGIVGQEVMKACSGKFHPLFQFFYFDSVES 191
DG+LE+++ +LLR FAFGARAVLNPMAAMFGGIVGQEV+KACSGKFHPL+QFFYFDSVES
Sbjct: 398 DGKLEEIDQKLLRNFAFGARAVLNPMAAMFGGIVGQEVVKACSGKFHPLYQFFYFDSVES 457
Query: 192 LPTEPLDPNDLKPINSRYDAQISVFGQKLQKKLEDAIVFVVGSGALGCEFLKNLALMGVS 371
LPT PLDPNDLKP+NSRYDAQISVFG KLQKKLE+A FVVGSGALGCEFLKNLALMGV
Sbjct: 458 LPTAPLDPNDLKPLNSRYDAQISVFGNKLQKKLEEAKAFVVGSGALGCEFLKNLALMGVC 517
Query: 372 CGSQGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAAAINPRLNIEALQNRVG 551
CG +GKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAA+AA+ INPR++IEALQNR
Sbjct: 518 CGDKGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAAAAASLINPRIHIEALQNRAS 577
Query: 552 PETENVFHDAFWENLNVVINALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPH 731
PETE+VF D FWENL+VVINALDNVNARLY+DQRCLYFQKPLLESGTLGAKCNTQMVIPH
Sbjct: 578 PETESVFDDTFWENLSVVINALDNVNARLYIDQRCLYFQKPLLESGTLGAKCNTQMVIPH 637
Query: 732 LTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPAEVNAYLSNPSEY 911
LTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTP EVNAYL NPS+Y
Sbjct: 638 LTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTEVNAYLINPSDY 697
Query: 912 TNGMKNAGDAQARDNLDRILECLDKEKCETFEDCITWARLKFEDYFADRVKQLTYTFPED 1091
+ M+ AGDAQARD LDR+LECLDKE+C+TF+DCITWARL+FEDYFADRVKQLT+TFPE+
Sbjct: 698 ISAMQKAGDAQARDTLDRVLECLDKERCDTFQDCITWARLRFEDYFADRVKQLTFTFPEE 757
Query: 1092 AMTSTG 1109
A TS+G
Sbjct: 758 ATTSSG 763
Score = 33.5 bits (75), Expect = 6.1
Identities = 19/56 (33%), Positives = 31/56 (54%), Gaps = 1/56 (1%)
Frame = +3
Query: 204 PLDPNDLKPINSR-YDAQISVFGQKLQKKLEDAIVFVVGSGALGCEFLKNLALMGV 368
P+D + I+ + Q++V+G++ ++L + V V G LG E KNL L GV
Sbjct: 64 PIDARNSPDIDEDLHSRQLAVYGRETMRRLFASNVLVSGLQGLGAEIAKNLILAGV 119
>ref|NP_565693.1| ubiquitin activating enzyme 1 (UBA1); protein id: At2g30110.1,
supported by cDNA: gi_19699086 [Arabidopsis thaliana]
gi|7437978|pir||T00587 probable ubiquitin-protein ligase
(EC 6.3.2.19) - Arabidopsis thaliana
gi|1750376|gb|AAB39246.1| ubiquitin activating enzyme
[Arabidopsis thaliana] gi|3150409|gb|AAC16961.1|
ubiquitin activating enzyme 1 (UBA1) [Arabidopsis
thaliana]
Length = 1080
Score = 661 bits (1706), Expect = 0.0
Identities = 318/367 (86%), Positives = 341/367 (92%)
Frame = +3
Query: 9 GDGRLEDVNPQLLRQFAFGARAVLNPMAAMFGGIVGQEVMKACSGKFHPLFQFFYFDSVE 188
GD ++E+V+ +LLR F+FGA+AVLNPMAAMFGGIVGQEV+KACSGKFHPLFQFFYFDSVE
Sbjct: 397 GDLKVENVDQKLLRHFSFGAKAVLNPMAAMFGGIVGQEVVKACSGKFHPLFQFFYFDSVE 456
Query: 189 SLPTEPLDPNDLKPINSRYDAQISVFGQKLQKKLEDAIVFVVGSGALGCEFLKNLALMGV 368
SLP+EP+D +D P NSRYDAQISVFG K QKKLEDA VF VGSGALGCEFLKNLALMGV
Sbjct: 457 SLPSEPVDSSDFAPRNSRYDAQISVFGAKFQKKLEDAKVFTVGSGALGCEFLKNLALMGV 516
Query: 369 SCGSQGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAAAINPRLNIEALQNRV 548
SCGSQGKLT+TDDD+IEKSNLSRQFLFRDWNIGQAKSTVAASAAA INPR NIEALQNRV
Sbjct: 517 SCGSQGKLTVTDDDIIEKSNLSRQFLFRDWNIGQAKSTVAASAAAVINPRFNIEALQNRV 576
Query: 549 GPETENVFHDAFWENLNVVINALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIP 728
G ETENVF DAFWENL VV+NALDNVNARLYVD RCLYFQKPLLESGTLG KCNTQ VIP
Sbjct: 577 GAETENVFDDAFWENLTVVVNALDNVNARLYVDSRCLYFQKPLLESGTLGTKCNTQSVIP 636
Query: 729 HLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPAEVNAYLSNPSE 908
HLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPAEVNAYLS+P E
Sbjct: 637 HLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPAEVNAYLSSPVE 696
Query: 909 YTNGMKNAGDAQARDNLDRILECLDKEKCETFEDCITWARLKFEDYFADRVKQLTYTFPE 1088
YTN M +AGDAQARD L+RI+ECL+KEKCETF+DC+TWARL+FEDYF +RVKQL YTFPE
Sbjct: 697 YTNSMMSAGDAQARDTLERIVECLEKEKCETFQDCLTWARLRFEDYFVNRVKQLIYTFPE 756
Query: 1089 DAMTSTG 1109
DA TSTG
Sbjct: 757 DAATSTG 763
Score = 49.7 bits (117), Expect = 8e-05
Identities = 28/120 (23%), Positives = 62/120 (51%)
Frame = +3
Query: 243 YDAQISVFGQKLQKKLEDAIVFVVGSGALGCEFLKNLALMGVSCGSQGKLTITDDDVIEK 422
+ Q++V+G++ ++L + V + G LG E KNL L GV +T+ D+ V+E
Sbjct: 78 HSRQLAVYGRETMRRLFASNVLISGMHGLGAEIAKNLILAGVK-----SVTLHDERVVEL 132
Query: 423 SNLSRQFLFRDWNIGQAKSTVAASAAAAINPRLNIEALQNRVGPETENVFHDAFWENLNV 602
+LS F+F + ++G+ ++ + +N + + +L + E + F + ++++
Sbjct: 133 WDLSSNFVFSEDDVGKNRADASVQKLQDLNNAVVVSSLTKSLNKEDLSGFQVVVFSDISM 192
>gb|AAL90910.1| At2g30110/T27E13.15 [Arabidopsis thaliana]
Length = 1080
Score = 661 bits (1706), Expect = 0.0
Identities = 318/367 (86%), Positives = 341/367 (92%)
Frame = +3
Query: 9 GDGRLEDVNPQLLRQFAFGARAVLNPMAAMFGGIVGQEVMKACSGKFHPLFQFFYFDSVE 188
GD ++E+V+ +LLR F+FGA+AVLNPMAAMFGGIVGQEV+KACSGKFHPLFQFFYFDSVE
Sbjct: 397 GDLKVENVDQKLLRHFSFGAKAVLNPMAAMFGGIVGQEVVKACSGKFHPLFQFFYFDSVE 456
Query: 189 SLPTEPLDPNDLKPINSRYDAQISVFGQKLQKKLEDAIVFVVGSGALGCEFLKNLALMGV 368
SLP+EP+D +D P NSRYDAQISVFG K QKKLEDA VF VGSGALGCEFLKNLALMGV
Sbjct: 457 SLPSEPVDSSDFAPRNSRYDAQISVFGAKFQKKLEDAKVFTVGSGALGCEFLKNLALMGV 516
Query: 369 SCGSQGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAAAINPRLNIEALQNRV 548
SCGSQGKLT+TDDD+IEKSNLSRQFLFRDWNIGQAKSTVAASAAA INPR NIEALQNRV
Sbjct: 517 SCGSQGKLTVTDDDIIEKSNLSRQFLFRDWNIGQAKSTVAASAAAVINPRFNIEALQNRV 576
Query: 549 GPETENVFHDAFWENLNVVINALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIP 728
G ETENVF DAFWENL VV+NALDNVNARLYVD RCLYFQKPLLESGTLG KCNTQ VIP
Sbjct: 577 GAETENVFDDAFWENLTVVVNALDNVNARLYVDSRCLYFQKPLLESGTLGTKCNTQSVIP 636
Query: 729 HLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPAEVNAYLSNPSE 908
HLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPAEVNAYLS+P E
Sbjct: 637 HLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPAEVNAYLSSPVE 696
Query: 909 YTNGMKNAGDAQARDNLDRILECLDKEKCETFEDCITWARLKFEDYFADRVKQLTYTFPE 1088
YTN M +AGDAQARD L+RI+ECL+KEKCETF+DC+TWARL+FEDYF +RVKQL YTFPE
Sbjct: 697 YTNSMMSAGDAQARDTLERIVECLEKEKCETFQDCLTWARLRFEDYFVNRVKQLIYTFPE 756
Query: 1089 DAMTSTG 1109
DA TSTG
Sbjct: 757 DAATSTG 763
Score = 49.7 bits (117), Expect = 8e-05
Identities = 28/120 (23%), Positives = 62/120 (51%)
Frame = +3
Query: 243 YDAQISVFGQKLQKKLEDAIVFVVGSGALGCEFLKNLALMGVSCGSQGKLTITDDDVIEK 422
+ Q++V+G++ ++L + V + G LG E KNL L GV +T+ D+ V+E
Sbjct: 78 HSRQLAVYGRETMRRLFASNVLISGMHGLGAEIAKNLILAGVK-----SVTLHDERVVEL 132
Query: 423 SNLSRQFLFRDWNIGQAKSTVAASAAAAINPRLNIEALQNRVGPETENVFHDAFWENLNV 602
+LS F+F + ++G+ ++ + +N + + +L + E + F + ++++
Sbjct: 133 WDLSSNFVFSEDDVGKNRADASVQKLQDLNNAVVVSSLTKSLNKEDLSGFQVVVFSDISM 192
>ref|NP_568168.1| ubiquitin activating enzyme 2 (UBA2); protein id: At5g06460.1
[Arabidopsis thaliana] gi|1703478|gb|AAB37569.1|
ubiquitin activating enzyme 2 gi|9758426|dbj|BAB08968.1|
ubiquitin activating enzyme 2 [Arabidopsis thaliana]
Length = 1077
Score = 658 bits (1698), Expect = 0.0
Identities = 316/367 (86%), Positives = 340/367 (92%)
Frame = +3
Query: 9 GDGRLEDVNPQLLRQFAFGARAVLNPMAAMFGGIVGQEVMKACSGKFHPLFQFFYFDSVE 188
GD RLEDVN +LLR AFG+RAVLNPMAAMFGGIVGQEV+KACSGKFHP+FQFFYFDSVE
Sbjct: 394 GDARLEDVNSKLLRHLAFGSRAVLNPMAAMFGGIVGQEVVKACSGKFHPIFQFFYFDSVE 453
Query: 189 SLPTEPLDPNDLKPINSRYDAQISVFGQKLQKKLEDAIVFVVGSGALGCEFLKNLALMGV 368
SLP EPLD ++ +P NSRYDAQISVFG LQKKLEDA VFVVG+GALGCEFLKNLALMGV
Sbjct: 454 SLPKEPLDASEFRPQNSRYDAQISVFGSTLQKKLEDARVFVVGAGALGCEFLKNLALMGV 513
Query: 369 SCGSQGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAAAINPRLNIEALQNRV 548
SCG+QGKLT+TDDDVIEKSNLSRQFLFRDWNIGQAKSTVAA+AAA IN RLNI+ALQNRV
Sbjct: 514 SCGTQGKLTVTDDDVIEKSNLSRQFLFRDWNIGQAKSTVAATAAAGINSRLNIDALQNRV 573
Query: 549 GPETENVFHDAFWENLNVVINALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIP 728
GPETENVF D+FWENL VV+NALDNV ARLYVD RC+YFQKPLLESGTLGAKCNTQMVIP
Sbjct: 574 GPETENVFDDSFWENLTVVVNALDNVTARLYVDSRCVYFQKPLLESGTLGAKCNTQMVIP 633
Query: 729 HLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPAEVNAYLSNPSE 908
HLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPAEVNAYLS+P E
Sbjct: 634 HLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPAEVNAYLSDPVE 693
Query: 909 YTNGMKNAGDAQARDNLDRILECLDKEKCETFEDCITWARLKFEDYFADRVKQLTYTFPE 1088
Y M+ AGDAQARD L R++ECL+KEKC +F+DCITWARL+FEDYFA+RVKQL YTFPE
Sbjct: 694 YMKAMRTAGDAQARDTLGRVVECLEKEKCNSFQDCITWARLRFEDYFANRVKQLCYTFPE 753
Query: 1089 DAMTSTG 1109
DA TSTG
Sbjct: 754 DAATSTG 760
Score = 51.2 bits (121), Expect = 3e-05
Identities = 28/105 (26%), Positives = 56/105 (52%)
Frame = +3
Query: 243 YDAQISVFGQKLQKKLEDAIVFVVGSGALGCEFLKNLALMGVSCGSQGKLTITDDDVIEK 422
+ Q++V+G++ +KL + V + G LG E KN+ L GV +T+ D++V+E
Sbjct: 75 HSRQLAVYGRETMRKLFASNVLISGMQGLGVEIAKNIILAGVK-----SVTLHDENVVEL 129
Query: 423 SNLSRQFLFRDWNIGQAKSTVAASAAAAINPRLNIEALQNRVGPE 557
+LS F+F + +IG+ ++ + +N + + L ++ E
Sbjct: 130 WDLSSNFVFTEEDIGKNRALASVHKLQELNNAVAVSTLTGKLTKE 174
>sp|P20973|UBA1_WHEAT Ubiquitin-activating enzyme E1 1 gi|100841|pir||A38373
ubiquitin-protein ligase (EC 6.3.2.19) E1 - wheat
gi|170780|gb|AAA34308.1| ubiquitin-activating enzyme E1
Length = 1051
Score = 634 bits (1636), Expect = 0.0
Identities = 296/367 (80%), Positives = 336/367 (90%)
Frame = +3
Query: 9 GDGRLEDVNPQLLRQFAFGARAVLNPMAAMFGGIVGQEVMKACSGKFHPLFQFFYFDSVE 188
GD +LE+++ +LL FA G+RAVLNPMAAMFGGIVGQEV+KACSGKFHPL+QFFYFDSVE
Sbjct: 367 GDRKLEEIDKKLLHHFASGSRAVLNPMAAMFGGIVGQEVVKACSGKFHPLYQFFYFDSVE 426
Query: 189 SLPTEPLDPNDLKPINSRYDAQISVFGQKLQKKLEDAIVFVVGSGALGCEFLKNLALMGV 368
SLP +PL+P DLKP NSRYDAQISVFG KLQ KLE+A +F+VGSGALGCEFLKNLALMG+
Sbjct: 427 SLPVDPLEPGDLKPKNSRYDAQISVFGSKLQNKLEEAKIFMVGSGALGCEFLKNLALMGI 486
Query: 369 SCGSQGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAAAINPRLNIEALQNRV 548
SC G LT+TDDDVIEKSNLSRQFLFRDWNIGQ KSTVAA+AA INP+L++EALQNR
Sbjct: 487 SCSQNGNLTLTDDDVIEKSNLSRQFLFRDWNIGQPKSTVAATAAMVINPKLHVEALQNRA 546
Query: 549 GPETENVFHDAFWENLNVVINALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIP 728
PETENVF+DAFWENL+ V+NALDNV AR+Y+D RC+YFQKPLLESGTLGAKCNTQMVIP
Sbjct: 547 SPETENVFNDAFWENLDAVVNALDNVTARMYIDSRCVYFQKPLLESGTLGAKCNTQMVIP 606
Query: 729 HLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPAEVNAYLSNPSE 908
HLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTP EVNA+LSNP+
Sbjct: 607 HLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTEVNAFLSNPTT 666
Query: 909 YTNGMKNAGDAQARDNLDRILECLDKEKCETFEDCITWARLKFEDYFADRVKQLTYTFPE 1088
Y + + AGDAQARD L+R++ECLD++KCETF+D ITWARLKFEDYF++RVKQLT+TFPE
Sbjct: 667 YISAARTAGDAQARDQLERVIECLDRDKCETFQDSITWARLKFEDYFSNRVKQLTFTFPE 726
Query: 1089 DAMTSTG 1109
D+MTS+G
Sbjct: 727 DSMTSSG 733
Score = 49.7 bits (117), Expect = 8e-05
Identities = 31/120 (25%), Positives = 58/120 (47%)
Frame = +3
Query: 243 YDAQISVFGQKLQKKLEDAIVFVVGSGALGCEFLKNLALMGVSCGSQGKLTITDDDVIEK 422
+ Q++V+G++ K+L + V V G LG E KNL L GV +T+ DD +E
Sbjct: 48 HSRQLAVYGRETMKRLFGSNVLVSGLQGLGAEIAKNLVLAGVK-----SVTLHDDGNVEL 102
Query: 423 SNLSRQFLFRDWNIGQAKSTVAASAAAAINPRLNIEALQNRVGPETENVFHDAFWENLNV 602
+LS F + ++GQ ++ +N + + AL + E + F + ++++
Sbjct: 103 WDLSSNFFLSENDVGQNRAQACVQKLQELNNAVLVSALTGDLTKEHLSKFQAVVFTDISL 162
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 949,455,970
Number of Sequences: 1393205
Number of extensions: 21572880
Number of successful extensions: 57169
Number of sequences better than 10.0: 425
Number of HSP's better than 10.0 without gapping: 53779
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 56675
length of database: 448,689,247
effective HSP length: 125
effective length of database: 274,538,622
effective search space used: 66987423768
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
