>KMC012809A_C01 KMC012809A_c01
ggatagaactcagaatttctgaggtcgatggttatggaatattaaagaaaaagatgagca
caaggctctacaataggaaaTACTCCAGAGTCTATGACTCCTTACCAGAGAGATATTCTC
TCCTTAACCTAATTCTCACCAACAAACATGCCTCCTTTCTCCTCCCCTCCTCCCTCTTAT
ATAATCCCTATTAACCTAAAATAACCTAACTGCCACATAGGCTACATATATTTATTTTAA
ATATATAAAATAACAATAGTACTAATTATTAATAACCCCATCCCACTAATCAAGGAAATC
CCCCCTAATAAACTGAATCCCACTAATCAAGTGAATCATGAATCCTCATATCTGGTGTCC
TATCAGAAGCTCCGAAGGCCAACGACGCCGTTCTGTTCTACAAGGCCGCGTTTGGTGCTG
AGGAAGTTAGCCGTGCTCTGAACCCTAAGCGCAAAGCTGACCATGAGCTTCCTCTCATCC
TCTCTGCTGAGCTCAAGATCGCTGGCTCCTCCATTCTCGTTTCTGACCTCGCTGTTGACT
CTACTGCACCAGCGAAGACTGGGGGAAACGGTATCGTTTTGTGCCTGGAGACTGAGGACG
TGGAAGCTGCCGTTGCTAAGGCTGTGCGCGCAGGTGCCGTTAAGGAAGGTGAGGTGGTTG
AAGGTGAAGGCGGCGCGTGTTGCGGTGGGCTCGTGGTGAAGGTGAAGGACCCGTACGGTT
TCATTTGGCAGATCTGCTCTCCAGTGAAGGCGAGTGCTGAGGTGGCAGCTTGATAACCGT
CAGATCTGTTAGTGGTGGTGATGTTCCTGACTAGTAATTTCAAATTTGGATTTAGGAGTA
GTTTGGTTAATGGATAAAAAGGACCTAATTGGTTTCAGAAAAGACnAATCTATGATTCGG
TTTAGGATATTTTGTTAGACGAAGGCATTGGTAGTAGTatttattaatattttgatatca
gtgcttattttatgctatgttttgaatgacccgagaggagaaaccactttatgttctt

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC012809A_C01 KMC012809A_c01
         (1018 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAO43000.1| early tobacco anther 1 [Nicotiana tabacum]             165  9e-40
ref|NP_568698.1| expressed protein; protein id: At5g48480.1, sup...   107  3e-22
ref|NP_436346.1| Hypothetical protein [Sinorhizobium meliloti] g...    56  8e-07
ref|NP_745582.1| glyoxalase family protein [Pseudomonas putida K...    55  1e-06
ref|NP_518228.1| CONSERVED HYPOTHETICAL PROTEIN [Ralstonia solan...    55  2e-06

>gb|AAO43000.1| early tobacco anther 1 [Nicotiana tabacum]
          Length = 166

 Score =  165 bits (418), Expect = 9e-40
 Identities = 87/131 (66%), Positives = 99/131 (75%), Gaps = 1/131 (0%)
 Frame = +3

Query: 366 EAPKANDAVLFYKAAFGAEEVSRALNPKRKADHELPLILSAELKIAGSSILVSDLAVD-S 542
           EAPKA DA  FYKAAFG EEV+R +N KRKA+ E+PL+ S ELK+  SS LVS+L  D S
Sbjct: 36  EAPKAKDAAQFYKAAFGVEEVTR-VNQKRKAEQEIPLVSSVELKLGSSSFLVSNLTDDDS 94

Query: 543 TAPAKTGGNGIVLCLETEDVEAAVAKAVRAGAVKEGEVVEGEGGACCGGLVVKVKDPYGF 722
           +AP K    G V CLETEDVEAAVAKAV AGAV EGE+ EG+G A  GG V K+KDPYG+
Sbjct: 95  SAPVKAATTGCVFCLETEDVEAAVAKAVSAGAVSEGEIAEGDGAAYFGGRVGKLKDPYGY 154

Query: 723 IWQICSPVKAS 755
           IW ICSPVK S
Sbjct: 155 IWMICSPVKKS 165

>ref|NP_568698.1| expressed protein; protein id: At5g48480.1, supported by cDNA:
           31667., supported by cDNA: gi_18389223 [Arabidopsis
           thaliana] gi|8777378|dbj|BAA96968.1|
           gene_id:MJE7.12~unknown protein [Arabidopsis thaliana]
           gi|18389224|gb|AAL67055.1| unknown protein [Arabidopsis
           thaliana] gi|21592710|gb|AAM64659.1| unknown
           [Arabidopsis thaliana] gi|22136970|gb|AAM91714.1|
           unknown protein [Arabidopsis thaliana]
          Length = 166

 Score =  107 bits (267), Expect = 3e-22
 Identities = 65/125 (52%), Positives = 81/125 (64%), Gaps = 1/125 (0%)
 Frame = +3

Query: 357 VLSEAPKANDAVLFYKAAFGAEEVSRALNPKRKADHELPLILSAELKIAGSSILVSDL-A 533
           +L EA K  DAV FYK+AFGA E   +L PKRK D ELP +LS+EL +AGSS +V D+ +
Sbjct: 30  LLVEAQKVGDAVTFYKSAFGAIESGHSLYPKRKLDQELPHVLSSELNLAGSSFVVCDVSS 89

Query: 534 VDSTAPAKTGGNGIVLCLETEDVEAAVAKAVRAGAVKEGEVVEGEGGACCGGLVVKVKDP 713
           +   + AK+ G+G+   L T+D EAAVAKAV AGAVK  EV E E      G   KV DP
Sbjct: 90  LPGFSTAKSEGSGVTFLLGTKDAEAAVAKAVDAGAVKV-EVTEAEVEL---GFKGKVTDP 145

Query: 714 YGFIW 728
           +G  W
Sbjct: 146 FGVTW 150

>ref|NP_436346.1| Hypothetical protein [Sinorhizobium meliloti]
           gi|25505078|pir||D95399 protein [imported] -
           Sinorhizobium meliloti (strain 1021) magaplasmid pSymA
           gi|14524256|gb|AAK65758.1| Hypothetical protein
           [Sinorhizobium meliloti]
          Length = 149

 Score = 56.2 bits (134), Expect = 8e-07
 Identities = 42/126 (33%), Positives = 63/126 (49%), Gaps = 2/126 (1%)
 Frame = +3

Query: 378 ANDAVLFYKAAFGAEEVSRALNPKRKADHELPLILSAELKIAGSSILVSDLAVD--STAP 551
           A  A+ FY  AFGAEE+ R  +P+         I  AELKI  S+++++D   D  + +P
Sbjct: 17  ARVAIDFYGRAFGAEELFRMTDPRDGR------IGHAELKIGESTVMIADEYPDFGALSP 70

Query: 552 AKTGGNGIVLCLETEDVEAAVAKAVRAGAVKEGEVVEGEGGACCGGLVVKVKDPYGFIWQ 731
              GG+ +   L T  V+A +A+AV AGAV     +        G  V  V DP+G  W 
Sbjct: 71  DTIGGSPVTFHLATLAVDADLARAVDAGAV----ALRAAADQGYGERVAMVVDPFGHRWM 126

Query: 732 ICSPVK 749
           +   ++
Sbjct: 127 LSQKIE 132

>ref|NP_745582.1| glyoxalase family protein [Pseudomonas putida KT2440]
           gi|24985094|gb|AAN69046.1|AE016537_4 glyoxalase family
           protein [Pseudomonas putida KT2440]
          Length = 159

 Score = 55.5 bits (132), Expect = 1e-06
 Identities = 42/120 (35%), Positives = 59/120 (49%), Gaps = 3/120 (2%)
 Frame = +3

Query: 378 ANDAVLFYKAAFGAEEVSRALNPKRKADHELPLILSAELKIAGSSILVS---DLAVDSTA 548
           A  A+ FYK AFGA E+ R   P  +  H       AEL+I  SS+++    D+    TA
Sbjct: 22  AAKAIEFYKQAFGAVEMFRLDAPGGRVGH-------AELRIGDSSLMLGDPCDMEGGLTA 74

Query: 549 PAKTGGNGIVLCLETEDVEAAVAKAVRAGAVKEGEVVEGEGGACCGGLVVKVKDPYGFIW 728
             K  G G+ L L  ED +   A+A+ AG ++   + +   G   G L    KDP+G IW
Sbjct: 75  SQKLNGAGVGLHLYVEDCDKVYAQALAAGGIQLHPLTDQFYGDRSGTL----KDPFGNIW 130

>ref|NP_518228.1| CONSERVED HYPOTHETICAL PROTEIN [Ralstonia solanacearum]
           gi|17427115|emb|CAD13635.1| CONSERVED HYPOTHETICAL
           PROTEIN [Ralstonia solanacearum]
          Length = 159

 Score = 54.7 bits (130), Expect = 2e-06
 Identities = 38/126 (30%), Positives = 60/126 (47%), Gaps = 2/126 (1%)
 Frame = +3

Query: 378 ANDAVLFYKAAFGAEEVSRALNPKRKADHELPLILSAELKIAGSSILVSDLA--VDSTAP 551
           A+ A+ FYK AFGA E+ R   P     H       AE++I  S ++++D    +   +P
Sbjct: 22  ADKALDFYKQAFGATEIMRMAGPNGAIAH-------AEIRIGDSPVMMADEMPNMSCASP 74

Query: 552 AKTGGNGIVLCLETEDVEAAVAKAVRAGAVKEGEVVEGEGGACCGGLVVKVKDPYGFIWQ 731
                  + L +  +DV+A  A A++ GA +   V     G   G L    KDP+G +W 
Sbjct: 75  DTLQSTSVGLMIYVKDVDAVFANAIKTGATEVRPVQNQFYGDRSGTL----KDPFGHVWT 130

Query: 732 ICSPVK 749
           I + V+
Sbjct: 131 ISTHVE 136

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 853,150,757
Number of Sequences: 1393205
Number of extensions: 19511170
Number of successful extensions: 81285
Number of sequences better than 10.0: 262
Number of HSP's better than 10.0 without gapping: 64699
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 78583
length of database: 448,689,247
effective HSP length: 124
effective length of database: 275,931,827
effective search space used: 59049410978
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 SPD041a10_f BP047230 1 517 2 MFB083g08_f BP040097 213 752 3 MPD096g01_f AV776303 217 785 4 SPD003d06_f BP044232 578 1018

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