>KMC012785A_C01 KMC012785A_c01
gcaataggggttaagaaatgcctGGGCAAAGGGAGAGCAAATAAAGTGAATCCAAGTACT
TGTACAATTAATTGAACCAATATATCAGGACTTCCAGTAAAGCCAATCCTCATTCATTCC
AACATACTGACTGACAACTTCCCCAGAGCCCCCCTCACTTCATTTTCGCTCTGCTTATCC
AGGGCAGTGCTGAGTTTTGAGAAGTAACAACTAACCCCATTCATAACAAATTCAATACAA
AAAAATCTAAGTTCAAACATAACATCCATTTATGGCTTCACATATTCTCTGATGAACTCT
TCCAGCTGAGATATCTCTCCAGTTAAACCAGAAGCATCTGCAGTGGTAACTTTGTTACAG
CACTGGGTAAAAGAAAATGCCTCACCAGGTACCCCCAACGTGAACTCATTTGTAACGTCA
GCATCTGACACTGCACTTCTTGATGCCCGTAATTTGTCAATTTCCTTCTCCAACTGTCTG
CGGATAAATTCCTTTGTATGAGCAGTGACTTTGTCTGTTTTGTTGCAGAGAATAAGCAGT
GGGATCTTCTTCTTTACCACACTTCCCTTAGTCAATATATCATATAGGTACTCTGAAGCA
GCACGGCAATTTGG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC012785A_C01 KMC012785A_c01
         (614 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_196186.1| signal recognition particle receptor beta subun...   185  5e-46
ref|NP_179467.1| putative signal recognition particle receptor b...   177  7e-44
ref|NP_701809.1| hypothetical protein [Plasmodium falciparum 3D7...    64  1e-09
ref|NP_788485.1| Signal recognition particle receptor beta CG331...    62  6e-09
ref|NP_033301.1| signal recognition particle receptor, B subunit...    60  2e-08

>ref|NP_196186.1| signal recognition particle receptor beta subunit-like protein;
           protein id: At5g05670.1 [Arabidopsis thaliana]
           gi|9759092|dbj|BAB09661.1| signal recognition particle
           receptor beta subunit-like protein [Arabidopsis
           thaliana]
          Length = 260

 Score =  185 bits (469), Expect = 5e-46
 Identities = 86/114 (75%), Positives = 102/114 (89%)
 Frame = -1

Query: 614 PNCRAASEYLYDILTKGSVVKKKIPLLILCNKTDKVTAHTKEFIRRQLEKEIDKLRASRS 435
           PNCRAASEYLY+ILT  +VVKKKIP+L+ CNKTDK+TAHTKEFIR+Q+EKEI+KLRASRS
Sbjct: 147 PNCRAASEYLYEILTNANVVKKKIPVLLCCNKTDKLTAHTKEFIRKQMEKEIEKLRASRS 206

Query: 434 AVSDADVTNEFTLGVPGEAFSFTQCCNKVTTADASGLTGEISQLEEFIREYVKP 273
           AVS AD+ N+FT+G+ GE FSFT C NKVT A+ASGLTGE  Q+E+FIREY+KP
Sbjct: 207 AVSTADIANDFTIGIEGEVFSFTHCSNKVTVAEASGLTGETIQIEDFIREYIKP 260

>ref|NP_179467.1| putative signal recognition particle receptor beta subunit; protein
           id: At2g18770.1, supported by cDNA: 14084. [Arabidopsis
           thaliana] gi|25411901|pir||D84568 hypothetical protein
           At2g18770 [imported] - Arabidopsis thaliana
           gi|4185143|gb|AAD08946.1| putative signal recognition
           particle receptor beta subunit [Arabidopsis thaliana]
           gi|21553377|gb|AAM62470.1| putative signal recognition
           particle receptor beta subunit [Arabidopsis thaliana]
          Length = 260

 Score =  177 bits (450), Expect = 7e-44
 Identities = 84/114 (73%), Positives = 98/114 (85%)
 Frame = -1

Query: 614 PNCRAASEYLYDILTKGSVVKKKIPLLILCNKTDKVTAHTKEFIRRQLEKEIDKLRASRS 435
           PN RAASEYLYDILT  SV+K K P+L+ CNKTDKVTAHTKEFIR+Q+EKEIDKLR SRS
Sbjct: 147 PNIRAASEYLYDILTNASVIKNKTPVLLCCNKTDKVTAHTKEFIRKQMEKEIDKLRVSRS 206

Query: 434 AVSDADVTNEFTLGVPGEAFSFTQCCNKVTTADASGLTGEISQLEEFIREYVKP 273
           A+S AD+ N+FTLG+ GE FSF+ C NKVT A+ASGLTGE  Q++EFIRE+VKP
Sbjct: 207 AISTADIANDFTLGIEGEVFSFSHCHNKVTVAEASGLTGETDQVQEFIREHVKP 260

>ref|NP_701809.1| hypothetical protein [Plasmodium falciparum 3D7]
           gi|23496982|gb|AAN36533.1|AE014851_52 hypothetical
           protein [Plasmodium falciparum 3D7]
          Length = 261

 Score = 64.3 bits (155), Expect = 1e-09
 Identities = 35/107 (32%), Positives = 58/107 (53%), Gaps = 2/107 (1%)
 Frame = -1

Query: 596 SEYLYDILTKGSVVKKKIPLLILCNKTDKVTAHTKEFIRRQLEKEIDKLRASRSAVSDAD 417
           +E L+++ T   +VKKKIP +I+CNKTD   +  K+ I+  LE+EI+ L+ S+    D D
Sbjct: 154 AEKLFELFTNKVIVKKKIPFIIVCNKTDLCNSRPKQVIKEDLEREIEILKMSKYNNLDDD 213

Query: 416 VTNEFT--LGVPGEAFSFTQCCNKVTTADASGLTGEISQLEEFIREY 282
             +E    LG   E F F +    +    AS     + ++ EF+ ++
Sbjct: 214 CIDETECFLGANSEFFRFEKAPCHIELCSASVKNNNVDEIIEFVEKH 260

>ref|NP_788485.1| Signal recognition particle receptor beta CG33162-PA
           gi|5901870|gb|AAD55443.1|AF181658_1 BcDNA.GM04779
           [Drosophila melanogaster] gi|23093878|gb|AAF50377.2|
           CG33162-PA [Drosophila melanogaster]
          Length = 244

 Score = 62.0 bits (149), Expect = 6e-09
 Identities = 35/109 (32%), Positives = 59/109 (54%), Gaps = 4/109 (3%)
 Frame = -1

Query: 605 RAASEYLYDILTKGSVVKKKIPLLILCNKTDKVTAHTKEFIRRQLEKEIDKLRASRS--- 435
           R  +++LY IL+  +   +   +L+LCNK D+ TA + + I+  LE E+  +R +RS   
Sbjct: 134 RDVADFLYTILSDSAT--QPCSVLVLCNKQDQTTAKSAQVIKSLLESELHTVRDTRSRKL 191

Query: 434 -AVSDADVTNEFTLGVPGEAFSFTQCCNKVTTADASGLTGEISQLEEFI 291
            +V D D +   TLG PG  F F+     +  A+AS    E+  L +++
Sbjct: 192 QSVGDEDGSKSITLGKPGRDFEFSHIAQNIQFAEASAKDTELDPLTDWL 240

>ref|NP_033301.1| signal recognition particle receptor, B subunit [Mus musculus]
           gi|1351115|sp|P47758|SRPB_MOUSE Signal recognition
           particle receptor beta subunit (SR-beta)
           gi|1363237|pir||A56487 signal recognition particle
           receptor beta chain - mouse gi|600886|gb|AAA69976.1|
           signal recognition particle receptor beta subunit
           gi|12836352|dbj|BAB23618.1| unnamed protein product [Mus
           musculus] gi|12841684|dbj|BAB25311.1| unnamed protein
           product [Mus musculus] gi|12853827|dbj|BAB29860.1|
           unnamed protein product [Mus musculus]
           gi|13277831|gb|AAH03798.1| signal recognition particle
           receptor, B subunit [Mus musculus]
           gi|26326439|dbj|BAC26963.1| unnamed protein product [Mus
           musculus]
          Length = 269

 Score = 60.1 bits (144), Expect = 2e-08
 Identities = 39/112 (34%), Positives = 58/112 (50%), Gaps = 10/112 (8%)
 Frame = -1

Query: 596 SEYLYDILTKGSVVKKKIPLLILCNKTDKVTAHTKEFIRRQLEKEIDKLRASRSAVS--- 426
           +E+LY +L     +K    LLI CNK D   A + + I++QLEKE++ LR +RSA     
Sbjct: 154 AEFLYQVLIDSMALKNSPSLLIACNKQDIAMAKSAKLIQQQLEKELNTLRVTRSAAPSTL 213

Query: 425 DADVTNEFTLGVPGEAFSFTQCCNKVTTADASGLTG-------EISQLEEFI 291
           D+  T    LG  G+ F F+Q   KV   + S   G       +I  LE+++
Sbjct: 214 DSSSTAPAQLGKKGKEFEFSQLPLKVEFLECSAKGGRGDTGSADIQDLEKWL 265

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 528,740,283
Number of Sequences: 1393205
Number of extensions: 11087804
Number of successful extensions: 27261
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 26614
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 27254
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 24854530794
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MF045f08_f BP030679 1 521 2 MF019h06_f BP029284 24 490 3 SPD011c11_f BP044865 25 612 4 SPD041f02_f BP047271 33 504 5 SPD053a06_f BP048200 43 507 6 MPD095e08_f AV776225 44 216 7 MFB095g10_f BP040951 48 621

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