Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC012739A_C01 KMC012739A_c01
(552 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAL66194.1|AF386512_1 cytochrome P450 [Pyrus communis] 191 5e-48
emb|CAC26932.1| ferulate-5-hydroxylase [Arabidopsis thaliana] gi... 156 2e-37
emb|CAC26936.1| ferulate-5-hydroxylase [Arabidopsis thaliana] gi... 156 2e-37
ref|NP_195345.1| ferulate-5-hydroxylase (FAH1); protein id: At4g... 156 2e-37
ref|NP_182079.1| cytochrome p450 family; protein id: At2g45550.1... 153 2e-36
>gb|AAL66194.1|AF386512_1 cytochrome P450 [Pyrus communis]
Length = 506
Score = 191 bits (485), Expect = 5e-48
Identities = 83/138 (60%), Positives = 106/138 (76%)
Frame = -1
Query: 552 PVAPLLIPHHAVEDCVVGEFFIPKNSRVIVNAWAVMRDPSAWTEPEKFWPERFEGSNIDL 373
PVAPLL+PH ++ED V + PK SR+ +N W + RDP +W E+F+PERF N+DL
Sbjct: 368 PVAPLLVPHQSMEDITVDGYHTPKKSRIFINIWTIGRDPKSWDNAEEFYPERFMNRNVDL 427
Query: 372 RGRDFQLIPFGSGRRGCPGLQLGLTMIRLVVAQLVHCFDWKLPNHMLPTDLDMTEEFGLT 193
RG DFQLIPFGSGRRGCP +QLGLT +RL + L+HC +W+LP+ MLP DLDMTE+FGL+
Sbjct: 428 RGHDFQLIPFGSGRRGCPAMQLGLTTVRLALGNLLHCSNWELPSGMLPKDLDMTEKFGLS 487
Query: 192 MPRAKHLVAIPTYRLQNE 139
+ +AKHL+A PT RL NE
Sbjct: 488 LSKAKHLLATPTCRLYNE 505
>emb|CAC26932.1| ferulate-5-hydroxylase [Arabidopsis thaliana]
gi|12578895|emb|CAC26933.1| ferulate-5-hydroxylase
[Arabidopsis thaliana]
Length = 520
Score = 156 bits (394), Expect = 2e-37
Identities = 73/136 (53%), Positives = 97/136 (70%), Gaps = 1/136 (0%)
Frame = -1
Query: 552 PVAPLLIPHHAVEDCVVGEFFIPKNSRVIVNAWAVMRDPSAWTEPEKFWPERFEGSNI-D 376
P PLL+ H ED + FFIPK SRV++NA+A+ RDP++WT+P+ F P RF + D
Sbjct: 382 PPIPLLL-HETAEDTSIDGFFIPKKSRVMINAFAIGRDPTSWTDPDTFRPSRFLEPGVPD 440
Query: 375 LRGRDFQLIPFGSGRRGCPGLQLGLTMIRLVVAQLVHCFDWKLPNHMLPTDLDMTEEFGL 196
+G +F+ IPFGSGRR CPG+QLGL + L VA ++HCF WKLP+ M P++LDM + FGL
Sbjct: 441 FKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHILHCFTWKLPDGMKPSELDMNDVFGL 500
Query: 195 TMPRAKHLVAIPTYRL 148
T P+A L A+PT RL
Sbjct: 501 TAPKATRLFAVPTTRL 516
>emb|CAC26936.1| ferulate-5-hydroxylase [Arabidopsis thaliana]
gi|12578903|emb|CAC26937.1| ferulate-5-hydroxylase
[Arabidopsis thaliana] gi|12578905|emb|CAC26938.1|
ferulate-5-hydroxylase [Arabidopsis thaliana]
gi|12578907|emb|CAC26939.1| ferulate-5-hydroxylase
[Arabidopsis thaliana] gi|12578909|emb|CAC26940.1|
ferulate-5-hydroxylase [Arabidopsis thaliana]
gi|12578911|emb|CAC26941.1| ferulate-5-hydroxylase
[Arabidopsis thaliana]
Length = 520
Score = 156 bits (394), Expect = 2e-37
Identities = 73/136 (53%), Positives = 97/136 (70%), Gaps = 1/136 (0%)
Frame = -1
Query: 552 PVAPLLIPHHAVEDCVVGEFFIPKNSRVIVNAWAVMRDPSAWTEPEKFWPERFEGSNI-D 376
P PLL+ H ED + FFIPK SRV++NA+A+ RDP++WT+P+ F P RF + D
Sbjct: 382 PPIPLLL-HETAEDTSIDGFFIPKKSRVMINAFAIGRDPTSWTDPDTFRPSRFLEPGVPD 440
Query: 375 LRGRDFQLIPFGSGRRGCPGLQLGLTMIRLVVAQLVHCFDWKLPNHMLPTDLDMTEEFGL 196
+G +F+ IPFGSGRR CPG+QLGL + L VA ++HCF WKLP+ M P++LDM + FGL
Sbjct: 441 FKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHILHCFTWKLPDGMKPSELDMNDVFGL 500
Query: 195 TMPRAKHLVAIPTYRL 148
T P+A L A+PT RL
Sbjct: 501 TAPKATRLFAVPTTRL 516
>ref|NP_195345.1| ferulate-5-hydroxylase (FAH1); protein id: At4g36220.1, supported
by cDNA: gi_1488254 [Arabidopsis thaliana]
gi|5921932|sp|Q42600|C84A_ARATH Cytochrome P450 84A1
(Ferulate-5-hydroxylase) (F5H) gi|7430674|pir||T04591
ferulate-5-hydroxylase (EC 1.-.-.-) - Arabidopsis
thaliana gi|1488255|gb|AAC49389.1|
ferulate-5-hydroxylase gi|2961381|emb|CAA18128.1|
ferulate-5-hydroxylase (FAH1) [Arabidopsis thaliana]
gi|3925365|gb|AAD11580.1| ferulate-5-hydroxylase
[Arabidopsis thaliana] gi|7270575|emb|CAB80293.1|
ferulate-5-hydroxylase (FAH1) [Arabidopsis thaliana]
gi|12578873|emb|CAC26922.1| ferulate-5-hydroxylase
[Arabidopsis thaliana] gi|12578875|emb|CAC26923.1|
ferulate-5-hydroxylase [Arabidopsis thaliana]
gi|12578877|emb|CAC26924.1| ferulate-5-hydroxylase
[Arabidopsis thaliana] gi|12578879|emb|CAC26925.1|
ferulate-5-hydroxylase [Arabidopsis thaliana]
gi|12578881|emb|CAC26926.1| ferulate-5-hydroxylase
[Arabidopsis thaliana] gi|12578883|emb|CAC26927.1|
ferulate-5-hydroxylase [Arabidopsis thaliana]
gi|12578885|emb|CAC26928.1| ferulate-5-hydroxylase
[Arabidopsis thaliana] gi|12578887|emb|CAC26929.1|
ferulate-5-hydroxylase [Arabidopsis thaliana]
gi|12578889|emb|CAC26930.1| ferulate-5-hydroxylase
[Arabidopsis thaliana] gi|12578891|emb|CAC26931.1|
ferulate-5-hydroxylase [Arabidopsis thaliana]
gi|12578897|emb|CAC26934.1| ferulate-5-hydroxylase
[Arabidopsis thaliana] gi|12578899|emb|CAC26935.1|
ferulate-5-hydroxylase [Arabidopsis thaliana]
Length = 520
Score = 156 bits (394), Expect = 2e-37
Identities = 73/136 (53%), Positives = 97/136 (70%), Gaps = 1/136 (0%)
Frame = -1
Query: 552 PVAPLLIPHHAVEDCVVGEFFIPKNSRVIVNAWAVMRDPSAWTEPEKFWPERFEGSNI-D 376
P PLL+ H ED + FFIPK SRV++NA+A+ RDP++WT+P+ F P RF + D
Sbjct: 382 PPIPLLL-HETAEDTSIDGFFIPKKSRVMINAFAIGRDPTSWTDPDTFRPSRFLEPGVPD 440
Query: 375 LRGRDFQLIPFGSGRRGCPGLQLGLTMIRLVVAQLVHCFDWKLPNHMLPTDLDMTEEFGL 196
+G +F+ IPFGSGRR CPG+QLGL + L VA ++HCF WKLP+ M P++LDM + FGL
Sbjct: 441 FKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHILHCFTWKLPDGMKPSELDMNDVFGL 500
Query: 195 TMPRAKHLVAIPTYRL 148
T P+A L A+PT RL
Sbjct: 501 TAPKATRLFAVPTTRL 516
>ref|NP_182079.1| cytochrome p450 family; protein id: At2g45550.1 [Arabidopsis
thaliana] gi|5915834|sp|O64635|C7C4_ARATH Cytochrome
P450 76C4 gi|7430677|pir||T00868 probable cytochrome
P450 [imported] - Arabidopsis thaliana
gi|2979547|gb|AAC06156.1| putative cytochrome P450
[Arabidopsis thaliana]
Length = 511
Score = 153 bits (386), Expect = 2e-36
Identities = 69/131 (52%), Positives = 92/131 (69%)
Frame = -1
Query: 552 PVAPLLIPHHAVEDCVVGEFFIPKNSRVIVNAWAVMRDPSAWTEPEKFWPERFEGSNIDL 373
P APLL+P A D V F +PK+++V+VN WA+ RDPS W P +F PERF G +ID+
Sbjct: 374 PAAPLLVPRKAESDVEVLGFMVPKDTQVLVNVWAIGRDPSVWENPSQFEPERFMGKDIDV 433
Query: 372 RGRDFQLIPFGSGRRGCPGLQLGLTMIRLVVAQLVHCFDWKLPNHMLPTDLDMTEEFGLT 193
+GRD++L PFG GRR CPGL L + + L++A L++ FDWKLPN ++ DLDM E FG+T
Sbjct: 434 KGRDYELTPFGGGRRICPGLPLAVKTVSLMLASLLYSFDWKLPNGVVSEDLDMDETFGIT 493
Query: 192 MPRAKHLVAIP 160
+ R L AIP
Sbjct: 494 LHRTNTLYAIP 504
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 491,259,798
Number of Sequences: 1393205
Number of extensions: 10663222
Number of successful extensions: 32082
Number of sequences better than 10.0: 2701
Number of HSP's better than 10.0 without gapping: 29607
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 30343
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 19234190289
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
