>KMC012693A_C01 KMC012693A_c01
ctctaaactgAAGGGTAGATTTAGAATGTAAAGTAAGTAATTACAGTTACTAATTTGAAA
ATTAACAACTGAGATATCATCATATGCATACACATGTATGAAATAACAAGGATACCATAT
AAGTACCATTATTTTCTATCATGTTGCTTTCTTCCATTTCTGTATTAATATTTGTATCAA
AAAAAATTTAAAAACTTCTTACTCCGTCCATGTTACATGCTTTTTTTATGCTAAGGATTT
GGGTATGCAGATTTGGATGATCTTGTAGTCAAAGAAGGCTTCATCTGAACTATATGTTCT
TCACTTACTCCCATTTTCCTTGCATAACGACCTGCTTCAAAGAACCTTTGGGCTGCATCA
TCCATGTAATCTTTCCCTTGCTTAACAGTCATGTCTTTCCCTATTGTGTCAGGGTAACTA
GTGAAGATGTTATCTCCTTTAATCACCGCCGCGAGTTGGATCAACTCCTGCTGAAATTCA
CTGAGCTTTGCATCTTCAT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC012693A_C01 KMC012693A_c01
         (499 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_180255.1| putative phospholipase C; protein id: At2g26870...    97  1e-19
gb|AAM74513.1| At1g07230/F10K1_4 [Arabidopsis thaliana] gi|24417...    95  4e-19
ref|NP_172203.1| unknown protein; protein id: At1g07230.1 [Arabi...    95  4e-19
dbj|BAB90779.1| B1139B11.7 [Oryza sativa (japonica cultivar-group)]    95  5e-19
ref|NP_190430.1| putative protein; protein id: At3g48610.1 [Arab...    87  1e-16

>ref|NP_180255.1| putative phospholipase C; protein id: At2g26870.1 [Arabidopsis
           thaliana] gi|7485404|pir||T02648 probable phospholipase
           C [imported] - Arabidopsis thaliana
           gi|3426039|gb|AAC32238.1| putative phospholipase C
           [Arabidopsis thaliana] gi|24417129|dbj|BAC22507.1|
           phosphatidylglycerol specific phospholipase C
           [Arabidopsis thaliana]
          Length = 514

 Score = 97.1 bits (240), Expect = 1e-19
 Identities = 48/79 (60%), Positives = 60/79 (75%)
 Frame = -3

Query: 497 EDAKLSEFQQELIQLAAVIKGDNIFTSYPDTIGKDMTVKQGKDYMDDAAQRFFEAGRYAR 318
           E A L+EFQQEL+QLAAV+KGDN+ T++P  I K MTV +GK YM+DA +RF EAGR A 
Sbjct: 434 EKALLTEFQQELVQLAAVLKGDNMLTTFPKEISKGMTVIEGKRYMEDAMKRFLEAGRMAL 493

Query: 317 KMGVSEEHIVQMKPSLTTR 261
            MG ++E +V MK SLT R
Sbjct: 494 SMGANKEELVHMKTSLTGR 512

>gb|AAM74513.1| At1g07230/F10K1_4 [Arabidopsis thaliana]
           gi|24417127|dbj|BAC22506.1| phosphatidylglycerol
           specific phospholipase C [Arabidopsis thaliana]
          Length = 533

 Score = 95.1 bits (235), Expect = 4e-19
 Identities = 50/87 (57%), Positives = 62/87 (70%)
 Frame = -3

Query: 497 EDAKLSEFQQELIQLAAVIKGDNIFTSYPDTIGKDMTVKQGKDYMDDAAQRFFEAGRYAR 318
           ED+KLSEFQ ELIQLA+ + GD++  SYPD IGK+MTV +G  Y +DA Q+F EAG  A 
Sbjct: 442 EDSKLSEFQVELIQLASQLVGDHLLNSYPD-IGKNMTVSEGNKYAEDAVQKFLEAGMAAL 500

Query: 317 KMGVSEEHIVQMKPSLTTRSSKSAYPN 237
           + G  E  IV M+PSLTTR+S S   N
Sbjct: 501 EAGADENTIVTMRPSLTTRTSPSEGTN 527

>ref|NP_172203.1| unknown protein; protein id: At1g07230.1 [Arabidopsis thaliana]
           gi|25406964|pir||E86207 hypothetical protein [imported]
           - Arabidopsis thaliana
           gi|8954023|gb|AAF82197.1|AC067971_5 Contains similarity
           to an unknown protein T8P19.120 gi|6523092 from
           Arabidopsis thaliana BAC T8P19 gb|AL133315.  ESTs
           gb|R84021, gb|AI992399, gb|H76814, gb|F15169,
           gb|AA585873, and gb|AA605516 come from this gene
          Length = 533

 Score = 95.1 bits (235), Expect = 4e-19
 Identities = 50/87 (57%), Positives = 62/87 (70%)
 Frame = -3

Query: 497 EDAKLSEFQQELIQLAAVIKGDNIFTSYPDTIGKDMTVKQGKDYMDDAAQRFFEAGRYAR 318
           ED+KLSEFQ ELIQLA+ + GD++  SYPD IGK+MTV +G  Y +DA Q+F EAG  A 
Sbjct: 442 EDSKLSEFQVELIQLASQLVGDHLLNSYPD-IGKNMTVSEGNKYAEDAVQKFLEAGMAAL 500

Query: 317 KMGVSEEHIVQMKPSLTTRSSKSAYPN 237
           + G  E  IV M+PSLTTR+S S   N
Sbjct: 501 EAGADENTIVTMRPSLTTRTSPSEGTN 527

>dbj|BAB90779.1| B1139B11.7 [Oryza sativa (japonica cultivar-group)]
          Length = 520

 Score = 94.7 bits (234), Expect = 5e-19
 Identities = 47/81 (58%), Positives = 62/81 (76%)
 Frame = -3

Query: 497 EDAKLSEFQQELIQLAAVIKGDNIFTSYPDTIGKDMTVKQGKDYMDDAAQRFFEAGRYAR 318
           E+AKLSEFQQEL+QLA+V+ GD+  +S  DTI   M V++G  YM  A +RFFE G  A+
Sbjct: 434 EEAKLSEFQQELVQLASVLNGDHQLSSLQDTIRDRMNVREGIAYMRGAVKRFFETGMSAK 493

Query: 317 KMGVSEEHIVQMKPSLTTRSS 255
           +MGV +E IV+M+PSLTTR+S
Sbjct: 494 RMGVDDEQIVKMRPSLTTRTS 514

>ref|NP_190430.1| putative protein; protein id: At3g48610.1 [Arabidopsis thaliana]
           gi|11358440|pir||T46205 hypothetical protein T8P19.120 -
           Arabidopsis thaliana gi|6523092|emb|CAB62350.1| putative
           protein [Arabidopsis thaliana]
          Length = 462

 Score = 86.7 bits (213), Expect = 1e-16
 Identities = 42/79 (53%), Positives = 55/79 (69%)
 Frame = -3

Query: 497 EDAKLSEFQQELIQLAAVIKGDNIFTSYPDTIGKDMTVKQGKDYMDDAAQRFFEAGRYAR 318
           EDA LSEFQ E++QLAAV+ GD+  +S+P+ IGK MTVKQ  +Y+  A  RF  A + A 
Sbjct: 381 EDAALSEFQGEVVQLAAVLNGDHFLSSFPEEIGKKMTVKQAHEYVKGATSRFIRASKEAM 440

Query: 317 KMGVSEEHIVQMKPSLTTR 261
           K+G  +  IV M+ SLTTR
Sbjct: 441 KLGADKSAIVDMRSSLTTR 459

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 393,592,848
Number of Sequences: 1393205
Number of extensions: 8159290
Number of successful extensions: 18670
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 17993
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 18662
length of database: 448,689,247
effective HSP length: 114
effective length of database: 289,863,877
effective search space used: 14783057727
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MFBL032f09_f BP042875 1 406 2 MPDL059c05_f AV779490 11 467 3 MPD090h12_f AV775950 55 502

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