>KMC012583A_C01 KMC012583A_c01
gtggtacaaagaaTTTATTATTATGCCTCTTAATGTACAGTGATGGTTTTGAATAAAAAA
AGGAAGTAGAAGAACAAGATGAATAGAGCAAGTCATTAAATAATCTTAAAGACAACATAT
TTTACAAAGACCATTCAAATCCAACTCAAGAAGTGAGTTTGATTTGGATCAGTGCCTAGT
TCCTACACTCAGCAGGAATGGCCGTGAGGGAATCGCTTGAGATCATTGCAGTAGTTATAG
ATCATGAAGTATTTCTGAACCCATCTCAGTCTTCTTCTGCCATAAGCAGTCAAGTGCATT
TGTCTGATACTCAGCTCCAGAATTGTGGATATTGAAGACCCGATTGAAATGGAAGTGGCC
TTGAAGTTGCGGTAGTATGCTGTGAAGGGGTGCTTCGGACCAATCAGTTTTCACCAATCC
AGCGTCG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC012583A_C01 KMC012583A_c01
         (427 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

sp|P35694|BRU1_SOYBN Brassinosteroid-regulated protein BRU1 prec...    87  6e-17
gb|AAB18368.1| xyloglucan endotransglycosylase-related protein g...    80  6e-17
ref|NP_193149.1| xyloglucan endotransglycosylase (XTR7); protein...    80  6e-17
ref|NP_566738.1| xyloglucan endotransglycosylase, putative; prot...    78  4e-16
gb|AAM61021.1| xyloglucan endotransglycosylase, putative [Arabid...    75  3e-15

>sp|P35694|BRU1_SOYBN Brassinosteroid-regulated protein BRU1 precursor
           gi|7447408|pir||T07678 xyloglucan
           endo-1,4-beta-D-glucanase (EC 3.2.1.-) BRU1 - soybean
           gi|347459|gb|AAA81350.1| brassinosteroid-regulated
           protein
          Length = 283

 Score = 86.7 bits (213), Expect = 6e-17
 Identities = 44/65 (67%), Positives = 45/65 (68%)
 Frame = -3

Query: 395 KHPFTAYYRNFKATSISIGSSISTILELSIRQMHLTAYGRRRLRWVQKYFMIYNYCNDLK 216
           K PFTAYYRNFKA   S  SSIS           L AY RRRLRWVQKYFMIYNYC+DLK
Sbjct: 213 KAPFTAYYRNFKAIEFSSKSSISNS-GAEYEANELDAYSRRRLRWVQKYFMIYNYCSDLK 271

Query: 215 RFPHG 201
           RFP G
Sbjct: 272 RFPQG 276

 Score = 42.0 bits (97), Expect = 0.002
 Identities = 21/50 (42%), Positives = 30/50 (60%)
 Frame = -2

Query: 420 GLVKTDWSEAPLHSILPQLQGHFHFNRVFNIHNSGAEYQTNALDCLWQKK 271
           GLVKTDWS+AP  +     +    F+   +I NSGAEY+ N LD   +++
Sbjct: 205 GLVKTDWSKAPFTAYYRNFKA-IEFSSKSSISNSGAEYEANELDAYSRRR 253

>gb|AAB18368.1| xyloglucan endotransglycosylase-related protein
           gi|15028017|gb|AAK76539.1| putative xyloglucan
           endotransglycosylase-related protein XTR-7 [Arabidopsis
           thaliana] gi|21592885|gb|AAM64835.1| xyloglucan
           endotransglycosylase-related protein XTR-7 [Arabidopsis
           thaliana]
          Length = 289

 Score = 80.5 bits (197), Expect(2) = 6e-17
 Identities = 40/71 (56%), Positives = 44/71 (61%), Gaps = 6/71 (8%)
 Frame = -3

Query: 395 KHPFTAYYRNFKATSISIGSSISTILELSIRQ------MHLTAYGRRRLRWVQKYFMIYN 234
           K PFTAYYR F A + +  S      + S           L AYGRRRLRWVQKYFMIYN
Sbjct: 209 KAPFTAYYRGFNAAACTASSGCDPKFKSSFGDGKLQVATELNAYGRRRLRWVQKYFMIYN 268

Query: 233 YCNDLKRFPHG 201
           YC+DLKRFP G
Sbjct: 269 YCSDLKRFPRG 279

 Score = 26.6 bits (57), Expect(2) = 6e-17
 Identities = 10/11 (90%), Positives = 11/11 (99%)
 Frame = -2

Query: 420 GLVKTDWSEAP 388
           GLVKTDWS+AP
Sbjct: 201 GLVKTDWSKAP 211

>ref|NP_193149.1| xyloglucan endotransglycosylase (XTR7); protein id: At4g14130.1,
           supported by cDNA: 33554., supported by cDNA:
           gi_1244759, supported by cDNA: gi_15028016 [Arabidopsis
           thaliana] gi|7447401|pir||F71402 xyloglucan
           endo-1,4-beta-D-glucanase (EC 3.2.1.-) XTR-7 -
           Arabidopsis thaliana gi|2244769|emb|CAB10192.1|
           xyloglucan endotransglycosylase-related protein XTR-7
           [Arabidopsis thaliana] gi|7268118|emb|CAB78455.1|
           xyloglucan endotransglycosylase-related protein XTR-7
           [Arabidopsis thaliana]
          Length = 289

 Score = 80.5 bits (197), Expect(2) = 6e-17
 Identities = 40/71 (56%), Positives = 44/71 (61%), Gaps = 6/71 (8%)
 Frame = -3

Query: 395 KHPFTAYYRNFKATSISIGSSISTILELSIRQ------MHLTAYGRRRLRWVQKYFMIYN 234
           K PFTAYYR F A + +  S      + S           L AYGRRRLRWVQKYFMIYN
Sbjct: 209 KAPFTAYYRGFNAAACTASSGCDPKFKSSFGDGKLQVATELNAYGRRRLRWVQKYFMIYN 268

Query: 233 YCNDLKRFPHG 201
           YC+DLKRFP G
Sbjct: 269 YCSDLKRFPRG 279

 Score = 26.6 bits (57), Expect(2) = 6e-17
 Identities = 10/11 (90%), Positives = 11/11 (99%)
 Frame = -2

Query: 420 GLVKTDWSEAP 388
           GLVKTDWS+AP
Sbjct: 201 GLVKTDWSKAP 211

>ref|NP_566738.1| xyloglucan endotransglycosylase, putative; protein id: At3g23730.1,
           supported by cDNA: 10852. [Arabidopsis thaliana]
           gi|9293946|dbj|BAB01849.1| endoxyloglucan
           endotransglycosylase [Arabidopsis thaliana]
          Length = 291

 Score = 77.8 bits (190), Expect(2) = 4e-16
 Identities = 41/75 (54%), Positives = 47/75 (62%), Gaps = 10/75 (13%)
 Frame = -3

Query: 395 KHPFTAYYRNFKATSISIGSSIS----------TILELSIRQMHLTAYGRRRLRWVQKYF 246
           K PFTAYYR F A + ++ S  S          T  E  +    L AYGRRRLRWVQKYF
Sbjct: 208 KAPFTAYYRGFNAAACTVSSGSSFCDPKFKSSFTNGESQVAN-ELNAYGRRRLRWVQKYF 266

Query: 245 MIYNYCNDLKRFPHG 201
           MIY+YC+DLKRFP G
Sbjct: 267 MIYDYCSDLKRFPQG 281

 Score = 26.6 bits (57), Expect(2) = 4e-16
 Identities = 10/11 (90%), Positives = 11/11 (99%)
 Frame = -2

Query: 420 GLVKTDWSEAP 388
           GLVKTDWS+AP
Sbjct: 200 GLVKTDWSKAP 210

>gb|AAM61021.1| xyloglucan endotransglycosylase, putative [Arabidopsis thaliana]
          Length = 291

 Score = 74.7 bits (182), Expect(2) = 3e-15
 Identities = 40/75 (53%), Positives = 46/75 (61%), Gaps = 10/75 (13%)
 Frame = -3

Query: 395 KHPFTAYYRNFKATSISIGSSIS----------TILELSIRQMHLTAYGRRRLRWVQKYF 246
           K PFTAYYR F A + ++ S  S          T  E  +    L AYG RRLRWVQKYF
Sbjct: 208 KAPFTAYYRGFNAAACTVSSGSSFCDPKFKSSFTNGESQVAN-ELNAYGIRRLRWVQKYF 266

Query: 245 MIYNYCNDLKRFPHG 201
           MIY+YC+DLKRFP G
Sbjct: 267 MIYDYCSDLKRFPQG 281

 Score = 26.6 bits (57), Expect(2) = 3e-15
 Identities = 10/11 (90%), Positives = 11/11 (99%)
 Frame = -2

Query: 420 GLVKTDWSEAP 388
           GLVKTDWS+AP
Sbjct: 200 GLVKTDWSKAP 210

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 349,128,142
Number of Sequences: 1393205
Number of extensions: 6606544
Number of successful extensions: 16108
Number of sequences better than 10.0: 95
Number of HSP's better than 10.0 without gapping: 15462
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 16052
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 6856422288
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MPD085f10_f AV775595 1 427 2 MFB090f02_f BP040586 13 161

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