>KMC012509A_C01 KMC012509A_c01
gtAGCAAAAGCACACCAATAATGGCGTTTAGCAAAAACATTTCCATCCATAAGCCTTTAG
TTGTGATATGGTGTATACATTGGACTCATATTATCGACACAAACTGTTGGCTATGTTTTT
AAAAAGCAAAAGTAGCACATAATATAATCACCACCTAAGATACAACACTCAGCGCTTGCG
GGCTCCGGCTGAATTTCAGATCCAACCTCAACAGATGATGAAACTTGTGGAGGAGAGCTT
TCAGGCTGAAGCAACAGGTTCTGGTACTTCTGGCTGAATTTCAGATTCAGCCGCAACAGA
TGATGAAACTTCTGGAGCGGTTTCAGGCTGAACAACAGATGATGGTAGTTCTGGCTCAAT
AAGCGCGGGTGCTTGAGTAATTTCCGGTGGCTTGAACTTGCTGATGTAGTAACTAAGTGA
TGGCCCGGAGTACTCAGCTCTCTGGATCGGAGGAGTAGTCTTACCAACTTGTAGAAGTAT
AGATGATGCTGCCGCAATTGAGATCAGGGCAAGGAATGCGACAATGCCTGCTGTGTTCT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC012509A_C01 KMC012509A_c01
         (539 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_568178.1| expressed protein; protein id: At5g07020.1, sup...    85  5e-16
gb|AAM62950.1| unknown [Arabidopsis thaliana]                          84  1e-15
ref|XP_220903.1| similar to nuclear domain 10 protein [Homo sapi...    39  0.040
emb|CAD38908.1| hypothetical protein [Homo sapiens]                    38  0.090
pir||T50624 hypothetical protein DKFZp762N1313.1 - human (fragment)    38  0.090

>ref|NP_568178.1| expressed protein; protein id: At5g07020.1, supported by cDNA:
           17482., supported by cDNA: gi_15293030, supported by
           cDNA: gi_20258892 [Arabidopsis thaliana]
           gi|9759558|dbj|BAB11160.1| gene_id:MOJ9.19~unknown
           protein [Arabidopsis thaliana]
           gi|15293031|gb|AAK93626.1| unknown protein [Arabidopsis
           thaliana] gi|20258893|gb|AAM14118.1| unknown protein
           [Arabidopsis thaliana]
          Length = 235

 Score = 85.1 bits (209), Expect = 5e-16
 Identities = 59/119 (49%), Positives = 71/119 (59%), Gaps = 14/119 (11%)
 Frame = -3

Query: 537 NTAGIVAFLALISIAAASSILLQVGKTTPPIQRA-EYSGPSLSYYISKFKP--------P 385
           NTA IVA +ALI++AAASSILLQVGK  P   +A +YSGPSLSYYI+KFKP        P
Sbjct: 126 NTAAIVAGIALIAVAAASSILLQVGKDAPTRPKAVDYSGPSLSYYINKFKPSEIVQPSTP 185

Query: 384 EITQAPALIE-----PELPSSVVQPETAPEVSSSVAAESEIQPEVPEPVASA*KLSSTS 223
            +T+AP + E     PE PS   Q  + PE  +     SE QPE     +S    SSTS
Sbjct: 186 SVTEAPPVAELETSLPETPSVAQQETSLPETMA-----SEAQPE----ASSVPTTSSTS 235

>gb|AAM62950.1| unknown [Arabidopsis thaliana]
          Length = 235

 Score = 84.0 bits (206), Expect = 1e-15
 Identities = 51/99 (51%), Positives = 60/99 (60%), Gaps = 9/99 (9%)
 Frame = -3

Query: 537 NTAGIVAFLALISIAAASSILLQVGKTTPPIQRA-EYSGPSLSYYISKFKP--------P 385
           NTA IVA +ALI++AAASSILLQVGK  P   +A +YSGPSLSYYI+KFKP        P
Sbjct: 126 NTAAIVAGIALIAVAAASSILLQVGKDAPTRPKAVDYSGPSLSYYINKFKPSEIVQPSTP 185

Query: 384 EITQAPALIEPELPSSVVQPETAPEVSSSVAAESEIQPE 268
            +T+AP + E E       P    E S      SE QPE
Sbjct: 186 SVTEAPPVAELETSLPETPPVAQQETSLPETMASEAQPE 224

>ref|XP_220903.1| similar to nuclear domain 10 protein [Homo sapiens] [Rattus
           norvegicus]
          Length = 974

 Score = 38.9 bits (89), Expect = 0.040
 Identities = 22/48 (45%), Positives = 30/48 (61%)
 Frame = -3

Query: 387 PEITQAPALIEPELPSSVVQPETAPEVSSSVAAESEIQPEVPEPVASA 244
           PE+  A A  EPE+PS+  Q +  PEV S   A+++ +PEVP P A A
Sbjct: 376 PEVPSAQADAEPEVPSA--QADAEPEVPS---AQADAEPEVPSPQADA 418

 Score = 38.5 bits (88), Expect = 0.053
 Identities = 23/55 (41%), Positives = 30/55 (53%), Gaps = 7/55 (12%)
 Frame = -3

Query: 387 PEITQAPALIEPELPSSVVQPETAPEVSSSVAAESEI-------QPEVPEPVASA 244
           PE+  A A  EPE+PS+  Q +  PE  S+  AE E+       +PEVP P A A
Sbjct: 583 PEVPSAQADAEPEVPSA--QADAEPEAPSAQVAEPEVPSAQADAEPEVPSPQADA 635

 Score = 35.4 bits (80), Expect(2) = 0.093
 Identities = 23/56 (41%), Positives = 29/56 (51%), Gaps = 8/56 (14%)
 Frame = -3

Query: 387 PEITQAPALIEPELPSSVVQPETAPEVSSSVA--------AESEIQPEVPEPVASA 244
           PE+  A A  EPE+PS   Q +  PEV S  A        A+ + +PEVP P A A
Sbjct: 615 PEVPSAQADAEPEVPSP--QADAEPEVPSPQADAEPEVPPAQVDAEPEVPSPQADA 668

 Score = 35.4 bits (80), Expect = 0.44
 Identities = 24/55 (43%), Positives = 31/55 (55%), Gaps = 7/55 (12%)
 Frame = -3

Query: 387 PEITQAPALIEPELPSSVVQPETAPEVSSSVA-AESEI------QPEVPEPVASA 244
           PE+    A  EPE+PS   Q +  PEV S+ A AE+E+      +PEVP P A A
Sbjct: 409 PEVPSPQADAEPEVPSP--QADAEPEVPSAQADAETEVPSQADAEPEVPSPQADA 461

 Score = 34.7 bits (78), Expect = 0.76
 Identities = 19/48 (39%), Positives = 27/48 (55%)
 Frame = -3

Query: 387 PEITQAPALIEPELPSSVVQPETAPEVSSSVAAESEIQPEVPEPVASA 244
           PE++   A  EPE+PS   Q +  PEV S    +++ +PEVP P   A
Sbjct: 692 PEVSSPQAAAEPEVPSP--QADAEPEVPS---PQADAEPEVPSPQVDA 734

 Score = 34.3 bits (77), Expect = 0.99
 Identities = 19/48 (39%), Positives = 28/48 (57%)
 Frame = -3

Query: 387 PEITQAPALIEPELPSSVVQPETAPEVSSSVAAESEIQPEVPEPVASA 244
           PE+  A A  EPE+PS+  Q +  PE  S   A+++++PE P   A A
Sbjct: 529 PEVPSAQADAEPEVPSA--QADAEPEAPS---AQADVEPEAPSAQADA 571

 Score = 33.9 bits (76), Expect = 1.3
 Identities = 24/56 (42%), Positives = 30/56 (52%), Gaps = 8/56 (14%)
 Frame = -3

Query: 387 PEITQAPALIEPELPSSVVQPETAPEVSS-SVAAESEI-------QPEVPEPVASA 244
           PE+  A    EPE+PS   Q +  PEVSS   AAE E+       +PEV  P A+A
Sbjct: 648 PEVPPAQVDAEPEVPSP--QADAEPEVSSPQAAAEPEVSSPQVDAEPEVSSPQAAA 701

 Score = 33.5 bits (75), Expect = 1.7
 Identities = 22/48 (45%), Positives = 27/48 (55%)
 Frame = -3

Query: 387 PEITQAPALIEPELPSSVVQPETAPEVSSSVAAESEIQPEVPEPVASA 244
           PE   A A  EPE+PS  V  E  PEV S   A+++ +PEVP   A A
Sbjct: 507 PEAPSAQADAEPEVPSPHVDAE--PEVPS---AQADAEPEVPSAQADA 549

 Score = 31.6 bits (70), Expect = 6.4
 Identities = 24/64 (37%), Positives = 30/64 (46%), Gaps = 16/64 (25%)
 Frame = -3

Query: 387 PEITQAPALIEPELPSSV--VQPE-------TAPEVSSSVAAESEI-------QPEVPEP 256
           PE+  A A  EPE PS+   V+PE         PE  S+  AE E+       +PEVP  
Sbjct: 540 PEVPSAQADAEPEAPSAQADVEPEAPSAQADAEPEAPSAQVAEPEVPSAQADAEPEVPSA 599

Query: 255 VASA 244
            A A
Sbjct: 600 QADA 603

 Score = 31.6 bits (70), Expect = 6.4
 Identities = 21/56 (37%), Positives = 29/56 (51%), Gaps = 8/56 (14%)
 Frame = -3

Query: 387 PEITQAPALIEPELPSSVVQPETAPEVSS--------SVAAESEIQPEVPEPVASA 244
           PE+  A A  EPE+PS  V  E  PEV S        + +A+++ +PE P   A A
Sbjct: 463 PEVPSAQADAEPEVPSPHVDAE--PEVPSPHIDAEPEAPSAQADAEPEAPSAQADA 516

 Score = 21.2 bits (43), Expect(2) = 0.093
 Identities = 9/17 (52%), Positives = 11/17 (63%)
 Frame = -1

Query: 248 QPESSPPQVSSSVEVGS 198
           +PE S PQV +  EV S
Sbjct: 680 EPEVSSPQVDAEPEVSS 696

>emb|CAD38908.1| hypothetical protein [Homo sapiens]
          Length = 504

 Score = 37.7 bits (86), Expect = 0.090
 Identities = 28/74 (37%), Positives = 35/74 (46%), Gaps = 1/74 (1%)
 Frame = -3

Query: 477 LLQVGKTTPPIQRAEYSGPSLSYYISKFKPPEITQAPALIEPELPSSVVQPETA-PEVSS 301
           LL  G   PP+  A  S P      S   PPE  +AP L  PE P+S ++PE A P +S+
Sbjct: 278 LLPGGGAPPPLSEA--SSPLACLLQSLQIPPEQPEAPCL-PPESPASALEPEPARPPLSA 334

Query: 300 SVAAESEIQPEVPE 259
                    P VPE
Sbjct: 335 LAPPHGSPDPPVPE 348

>pir||T50624 hypothetical protein DKFZp762N1313.1 - human (fragment)
          Length = 220

 Score = 37.7 bits (86), Expect = 0.090
 Identities = 28/74 (37%), Positives = 35/74 (46%), Gaps = 1/74 (1%)
 Frame = -3

Query: 477 LLQVGKTTPPIQRAEYSGPSLSYYISKFKPPEITQAPALIEPELPSSVVQPETA-PEVSS 301
           LL  G   PP+  A  S P      S   PPE  +AP L  PE P+S ++PE A P +S+
Sbjct: 45  LLPGGGAPPPLSEA--SSPLACLLQSLQIPPEQPEAPCL-PPESPASALEPEPARPPLSA 101

Query: 300 SVAAESEIQPEVPE 259
                    P VPE
Sbjct: 102 LAPPHGSPDPPVPE 115

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 442,982,506
Number of Sequences: 1393205
Number of extensions: 8999757
Number of successful extensions: 33107
Number of sequences better than 10.0: 108
Number of HSP's better than 10.0 without gapping: 29515
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 32798
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 18462123008
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 SPD062e02_f BP048944 1 358 2 MFB089c10_f BP040490 3 549 3 MFB100d05_f BP041258 34 442 4 MPD081h05_f AV775350 43 336

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