>KMC012453A_C01 KMC012453A_c01
tGGAAGCAAGCAAAATTCGAAGTTGAAAAAAAATGTAATTAAAAGGATAAATTTCAGATT
ACAACATGTCCATGGCATTAGAATATACTGATTTAGTTCATGGACAAACCAAACTAAACT
GAATTCAAGATCTATGTTCTCCAGCTCCGGTTACAATACAATCCCTCACTTCATGCAAAC
TTTCCTTGATCCAAAGTGAACTCCCAGCAACCAATAACAAGTCCTCAACCTTGAGCTTGG
GTTTCTATGCAAGAAAACCCCAACCTTGTTCAGGCCAACAACCATAAGGTCAACCTTATG
ATGTGTTTATCACCTACTGTCTCTCCTTTTCCATGTTGAACCTGGCCGTGATGATTTGGG
TGGTTCCTTACTCTTGTTAAACACTCTTGGTTTAAATTGTTTTTTCAGAATAAGGTTGCG
AACTTGTTTTTTCTTTTCTTCTGCCTTCAATTTGGTGAGACTGGGCTTGTATATAATCAA
TGTTCGACCAATCTGACTAACAGTCACTGAACCAGTTAATTCTTCCAACTGCTTCACCGC
ATCTTCTAACTCACCCGGACAAGTCCGATGTACTTTAATC

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC012453A_C01 KMC012453A_c01
         (580 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_179731.1| unknown protein; protein id: At2g21350.1 [Arabi...    79  5e-14
ref|NP_195615.1| putative protein; protein id: At4g39040.1 [Arab...    79  5e-14
gb|AAL35360.1|AF442732_3 reverse transcriptase/ribonuclease H [T...    32  4.4
ref|NP_009698.1| Inhibitory regulator of the RAS-cAMP pathway, n...    31  9.7
ref|NP_688654.1| conserved hypothetical protein TIGR00253 [Strep...    31  9.7

>ref|NP_179731.1| unknown protein; protein id: At2g21350.1 [Arabidopsis thaliana]
           gi|25412032|pir||C84600 hypothetical protein At2g21350
           [imported] - Arabidopsis thaliana
           gi|4567270|gb|AAD23683.1| unknown protein [Arabidopsis
           thaliana]
          Length = 151

 Score = 78.6 bits (192), Expect = 5e-14
 Identities = 35/52 (67%), Positives = 45/52 (86%)
 Frame = -2

Query: 579 IKVHRTCPGELEDAVKQLEELTGSVTVSQIGRTLIIYKPSLTKLKAEEKKKQ 424
           +K+HRTCPGELED + +LEE TGSV+V QI RT+I+Y+PS TKLKA+E KK+
Sbjct: 64  VKIHRTCPGELEDMILRLEEATGSVSVGQIARTVILYRPSPTKLKADEDKKR 115

>ref|NP_195615.1| putative protein; protein id: At4g39040.1 [Arabidopsis thaliana]
           gi|7485794|pir||T06064 hypothetical protein F19H22.140 -
           Arabidopsis thaliana gi|4539323|emb|CAB38824.1| putative
           protein [Arabidopsis thaliana]
           gi|7270887|emb|CAB80567.1| putative protein [Arabidopsis
           thaliana] gi|26452052|dbj|BAC43116.1| unknown protein
           [Arabidopsis thaliana] gi|28950859|gb|AAO63353.1|
           At4g39040 [Arabidopsis thaliana]
          Length = 296

 Score = 78.6 bits (192), Expect = 5e-14
 Identities = 43/91 (47%), Positives = 58/91 (63%), Gaps = 2/91 (2%)
 Frame = -2

Query: 579 IKVHRTCPGELEDAVKQLEELTGSVTVSQIGRTLIIYKPSLTKLKAEEKKKQVRNLILKK 400
           +K+ +T P ELEDAV+ LEE TGSV V QIGRT+I+Y+PS TK+KAE KKK+V  + + +
Sbjct: 202 VKIRKTSPDELEDAVQHLEEATGSVAVGQIGRTVILYRPSPTKMKAEAKKKEVERMSITR 261

Query: 399 QFKPRVFNKSK--EPPKSSRPGSTWKRRDSR 313
           + K      +K      S RP    +R  SR
Sbjct: 262 RQKYANTRPTKPFRREYSERPDGRGRRGGSR 292

>gb|AAL35360.1|AF442732_3 reverse transcriptase/ribonuclease H [Tetraodon nigroviridis]
          Length = 785

 Score = 32.3 bits (72), Expect = 4.4
 Identities = 23/81 (28%), Positives = 35/81 (42%)
 Frame = +2

Query: 236 LGFLCKKTPTLFRPTTIRSTL*CVYHLLSLLFHVEPGRDDLGGSLLLLNTLGLNCFFRIR 415
           LGF   +T +   P+     L    + LS    +   R+      L L  LG    FR+ 
Sbjct: 386 LGFTVNETKSRLEPSQYTDYLGLTLNSLSYRVRLSTEREQTFNHCLALFQLGKMVTFRLC 445

Query: 416 LRTCFFFSSAFNLVRLGLYII 478
           LR     +S  ++VRLGL ++
Sbjct: 446 LRLLGLMASVISVVRLGLLMM 466

>ref|NP_009698.1| Inhibitory regulator of the RAS-cAMP pathway, negatively regulates
            cAPK by antagonizing CDC25; Ira1p [Saccharomyces
            cerevisiae] gi|585325|sp|P18963|IRA1_YEAST Inhibitory
            regulator protein IRA1 gi|626168|pir||S46009
            GTPase-activating protein IRA1 - yeast (Saccharomyces
            cerevisiae) gi|536438|emb|CAA85098.1| ORF YBR140c
            [Saccharomyces cerevisiae]
          Length = 3092

 Score = 31.2 bits (69), Expect = 9.7
 Identities = 23/78 (29%), Positives = 38/78 (48%), Gaps = 3/78 (3%)
 Frame = +3

Query: 57   DYNMSMALEYTDLVHGQTKLN*IQDLCSPAPVTIQS---LTSCKLSLIQSELPATNNKSS 227
            D   S+  E T+++H  +  + +  L S +P++I S   +T+ +   IQS LP  +N+SS
Sbjct: 858  DAATSVYTEPTEIIHNSSDASLVSSL-SQSPLSINSGSNITNTRTWDIQSILPTLSNRSS 916

Query: 228  TLSLGFYARKPQPCSGQQ 281
               L        P   QQ
Sbjct: 917  ASDLSLSNILTNPLEAQQ 934

>ref|NP_688654.1| conserved hypothetical protein TIGR00253 [Streptococcus agalactiae
           2603V/R] gi|25011747|ref|NP_736142.1| Unknown
           [Streptococcus agalactiae NEM316]
           gi|22534696|gb|AAN00527.1|AE014267_10 conserved
           hypothetical protein TIGR00253 [Streptococcus agalactiae
           2603V/R] gi|24413287|emb|CAD47366.1| Unknown
           [Streptococcus agalactiae NEM316]
          Length = 105

 Score = 31.2 bits (69), Expect = 9.7
 Identities = 18/45 (40%), Positives = 26/45 (57%)
 Frame = -2

Query: 552 ELEDAVKQLEELTGSVTVSQIGRTLIIYKPSLTKLKAEEKKKQVR 418
           ++ D  + LE+  G  TV +IGR LI+YK S  K   E +K  V+
Sbjct: 57  DIHDVAEVLEDEIGCDTVLKIGRILILYKESARK---ENRKISVK 98

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 490,638,325
Number of Sequences: 1393205
Number of extensions: 10252791
Number of successful extensions: 27448
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 26737
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 27423
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 21426319650
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MF033d10_f BP030028 1 536 2 MPD078f06_f AV775135 95 602 3 SPD100d10_f BP051982 97 544 4 SPD100e11_f BP051989 97 597 5 MFB072e03_f BP039244 97 542 6 MF039f06_f BP030346 117 659 7 MFB055b11_f BP037975 119 631 8 MF016g01_f BP029106 151 421

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