Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC012376A_C01 KMC012376A_c01
(539 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAD29733.1| pectin methylesterase [Sesbania rostrata] 114 1e-24
pir||T06468 pectinesterase (EC 3.1.1.11) precursor - garden pea ... 111 5e-24
pir||T06374 probable pectinesterase (EC 3.1.1.11) precursor - ga... 108 3e-23
ref|NP_172624.1| pectin methylesterase, putative; protein id: At... 42 2e-07
gb|AAF16636.1|AC011661_14 T23J18.23 [Arabidopsis thaliana] 42 2e-07
>emb|CAD29733.1| pectin methylesterase [Sesbania rostrata]
Length = 554
Score = 114 bits (284), Expect = 1e-24
Identities = 59/87 (67%), Positives = 71/87 (80%), Gaps = 1/87 (1%)
Frame = +1
Query: 280 HVSEVSQS-LGSATKDPKLNILISLMTKSTSHIQEAMVKTKAIRNRINNPREEAAWSDCE 456
HVSEV+Q + + TKD KLN+LISL+T+ST IQ AM KAI+++INNP+EEAA DCE
Sbjct: 67 HVSEVAQGPILATTKDHKLNLLISLLTQSTPQIQNAMDTVKAIKHKINNPKEEAALHDCE 126
Query: 457 QLMDLSIDRVWDSVMALTKDKTDSHQD 537
QLMDLSIDRVWDSV+ALTK+ DS QD
Sbjct: 127 QLMDLSIDRVWDSVVALTKNTIDSQQD 153
Score = 92.4 bits (228), Expect = 3e-18
Identities = 55/88 (62%), Positives = 57/88 (64%), Gaps = 1/88 (1%)
Frame = +3
Query: 93 QQSLLDKPRKSFSKTFWLFLSLVAIISSSALMASYL-KPTSFNLFLSPPHGCEHALDASS 269
QQSLLDKPR S SKTFWL LSLVAIISSSAL+ASYL PTSF S PH CEHALD S
Sbjct: 4 QQSLLDKPRNSLSKTFWLILSLVAIISSSALIASYLFNPTSFFNLTSSPHVCEHALDTKS 63
Query: 270 CLASCFRSISKPRLSHKRSQTEYSHILN 353
CLA P L+ T H LN
Sbjct: 64 CLAHVSEVAQGPILA-----TTKDHKLN 86
>pir||T06468 pectinesterase (EC 3.1.1.11) precursor - garden pea
gi|2578440|emb|CAA47810.1| pectinesterase [Pisum
sativum]
Length = 554
Score = 111 bits (278), Expect = 5e-24
Identities = 68/139 (48%), Positives = 85/139 (60%), Gaps = 17/139 (12%)
Frame = +1
Query: 172 VPQPLWLLISNPPPSTSSC----HL-------PMAVNMHLTHH-----HA*RHVSEVSQS 303
+P+ WL++S SS HL P++ +L H HVSEV Q
Sbjct: 15 IPKTFWLILSLAAIIGSSALIVSHLNKPISFFPLSSAPNLCEHAVDTKSCLTHVSEVVQG 74
Query: 304 LGSA-TKDPKLNILISLMTKSTSHIQEAMVKTKAIRNRINNPREEAAWSDCEQLMDLSID 480
A TKD KL+ LISL+TKSTSHIQ+AM I+ R+N+PREE A +DCEQLMDLS+D
Sbjct: 75 QALANTKDHKLSTLISLLTKSTSHIQKAMETANVIKRRVNSPREETALNDCEQLMDLSMD 134
Query: 481 RVWDSVMALTKDKTDSHQD 537
RVWDSV+ LTK+ DS QD
Sbjct: 135 RVWDSVLTLTKNNIDSQQD 153
Score = 75.9 bits (185), Expect = 3e-13
Identities = 39/62 (62%), Positives = 46/62 (73%), Gaps = 1/62 (1%)
Frame = +3
Query: 93 QQSLLDKPRKSFSKTFWLFLSLVAIISSSALMASYL-KPTSFNLFLSPPHGCEHALDASS 269
Q++L+DKPRKS KTFWL LSL AII SSAL+ S+L KP SF S P+ CEHA+D S
Sbjct: 4 QETLIDKPRKSIPKTFWLILSLAAIIGSSALIVSHLNKPISFFPLSSAPNLCEHAVDTKS 63
Query: 270 CL 275
CL
Sbjct: 64 CL 65
>pir||T06374 probable pectinesterase (EC 3.1.1.11) precursor - garden pea
gi|3088646|gb|AAC14742.1| pectin methylesterase [Pisum
sativum] gi|3426335|gb|AAC32273.1| pectin methylesterase
[Pisum sativum]
Length = 554
Score = 108 bits (271), Expect = 3e-23
Identities = 66/139 (47%), Positives = 85/139 (60%), Gaps = 17/139 (12%)
Frame = +1
Query: 172 VPQPLWLLISNPPPSTSSC----HL-------PMAVNMHLTHH-----HA*RHVSEVSQS 303
+P+ WL++S SS HL P++ +L H HVSEV Q
Sbjct: 15 IPKTFWLILSLAAIIGSSALIVSHLNKPISFFPLSSAPNLCEHAVDTKSCLTHVSEVVQG 74
Query: 304 LGSA-TKDPKLNILISLMTKSTSHIQEAMVKTKAIRNRINNPREEAAWSDCEQLMDLSID 480
A TKD KL+ LISL+TKSTSHIQ+AM I+ R+N+P++E A +DCEQLMDLS+D
Sbjct: 75 QALANTKDHKLSTLISLLTKSTSHIQKAMETANVIKRRVNSPKKETALNDCEQLMDLSMD 134
Query: 481 RVWDSVMALTKDKTDSHQD 537
RVWDSV+ LTK+ DS QD
Sbjct: 135 RVWDSVLTLTKNNIDSQQD 153
Score = 74.7 bits (182), Expect = 7e-13
Identities = 38/62 (61%), Positives = 46/62 (73%), Gaps = 1/62 (1%)
Frame = +3
Query: 93 QQSLLDKPRKSFSKTFWLFLSLVAIISSSALMASYL-KPTSFNLFLSPPHGCEHALDASS 269
Q++L+DKP+KS KTFWL LSL AII SSAL+ S+L KP SF S P+ CEHA+D S
Sbjct: 4 QETLIDKPKKSIPKTFWLILSLAAIIGSSALIVSHLNKPISFFPLSSAPNLCEHAVDTKS 63
Query: 270 CL 275
CL
Sbjct: 64 CL 65
>ref|NP_172624.1| pectin methylesterase, putative; protein id: At1g11580.1, supported
by cDNA: gi_14334991 [Arabidopsis thaliana]
Length = 557
Score = 41.6 bits (96), Expect(2) = 2e-07
Identities = 21/66 (31%), Positives = 37/66 (55%)
Frame = +1
Query: 310 SATKDPKLNILISLMTKSTSHIQEAMVKTKAIRNRINNPREEAAWSDCEQLMDLSIDRVW 489
S +K +L++L + S ++ M R R N R++A ++DCE++MD+S DR+
Sbjct: 79 SLSKLNRLDLLHVFLKNSVWRLESTMTMVSEARIRSNGVRDKAGFADCEEMMDVSKDRMM 138
Query: 490 DSVMAL 507
S+ L
Sbjct: 139 SSMEEL 144
Score = 35.0 bits (79), Expect(2) = 2e-07
Identities = 28/84 (33%), Positives = 38/84 (44%), Gaps = 6/84 (7%)
Frame = +3
Query: 81 MATQQQSLLDKPRKSFSKTFWLFLSLVAIISSSALMASYLKPTSFN----LFLSPPHGCE 248
M+ Q LL KP+ K L LS VAI+ S A + L + N L+ C
Sbjct: 1 MSNSNQPLLSKPKSLKHKNLCLVLSFVAILGSVAFFTAQLISVNTNNNDDSLLTTSQICH 60
Query: 249 HALDASSC--LASCFRSISKPRLS 314
A D SC L S F ++S +L+
Sbjct: 61 GAHDQDSCQALLSEFTTLSLSKLN 84
>gb|AAF16636.1|AC011661_14 T23J18.23 [Arabidopsis thaliana]
Length = 376
Score = 41.6 bits (96), Expect(2) = 2e-07
Identities = 21/66 (31%), Positives = 37/66 (55%)
Frame = +1
Query: 310 SATKDPKLNILISLMTKSTSHIQEAMVKTKAIRNRINNPREEAAWSDCEQLMDLSIDRVW 489
S +K +L++L + S ++ M R R N R++A ++DCE++MD+S DR+
Sbjct: 100 SLSKLNRLDLLHVFLKNSVWRLESTMTMVSEARIRSNGVRDKAGFADCEEMMDVSKDRMM 159
Query: 490 DSVMAL 507
S+ L
Sbjct: 160 SSMEEL 165
Score = 35.0 bits (79), Expect(2) = 2e-07
Identities = 28/84 (33%), Positives = 38/84 (44%), Gaps = 6/84 (7%)
Frame = +3
Query: 81 MATQQQSLLDKPRKSFSKTFWLFLSLVAIISSSALMASYLKPTSFN----LFLSPPHGCE 248
M+ Q LL KP+ K L LS VAI+ S A + L + N L+ C
Sbjct: 22 MSNSNQPLLSKPKSLKHKNLCLVLSFVAILGSVAFFTAQLISVNTNNNDDSLLTTSQICH 81
Query: 249 HALDASSC--LASCFRSISKPRLS 314
A D SC L S F ++S +L+
Sbjct: 82 GAHDQDSCQALLSEFTTLSLSKLN 105
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 446,802,565
Number of Sequences: 1393205
Number of extensions: 8907561
Number of successful extensions: 23587
Number of sequences better than 10.0: 27
Number of HSP's better than 10.0 without gapping: 22965
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 23567
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 18462123008
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
