>KMC012059A_C01 KMC012059A_c01
gGAAAAAAAAATTTCAGGACTTATTTGGATACGGGGTACAATTTCAGGGGAGGCTACAGA
GTATTTAAGCCTTAAAAGAAACTAAGCTCGCAGAAACATATTTAAGTACAAAATGTAACT
GGTAGAAATTGTTCATACAAGAACGAAAATTAGCATCACCTGAAGCTACGCCTAGACACA
AAAGCACATCAATACACTTGAAACTGACATTTACTGAATAAACATGAGATTCGAAGAAGA
AACCCCAAAAGAGAAATGCATGGCTAAAACAGGGTTTATTCTTTCTCTCACATTTAAACG
AATACAGGGCAAGCAGATTTTATTTTCCTTCTCAAAAACCACAAACAACACTTTCATTTG
GTAGTAGTGCTTGCATCTTCATTTACTGAGGGATCTTGTTGAGCTTGCAGTAGGCATAGT
CCTTATATTTCTTGGTCTTGTATTTTGGTGGATTGTCTTGGTTAACCAACTTGGAGTGAG
GACCAACTTCATGGTCTCCTTGGGGCTCTATGAACACCGGCCATGACATTCTTGTCTCCT
CTTTGTTCACTGTTGTTCTGTGCAGGACAGCCTTATATTTCCCATTGCTCAGTATCTCCA
TTTGGTCTCCAATGTGAATGACGAGGG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC012059A_C01 KMC012059A_c01
         (627 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

sp|Q9M547|FLS_EUSGR Flavonol synthase (FLS) gi|7578883|gb|AAF641...   144  7e-34
sp|Q07512|FLS_PETHY Flavonol synthase (FLS) gi|421946|pir||S3351...   140  1e-32
sp|Q41452|FLS_SOLTU Flavonol synthase (FLS) gi|7433193|pir||T073...   139  3e-32
sp|Q9ZWQ9|FLS_CITUN Flavonol synthase (FLS) (CitFLS) gi|4126403|...   138  7e-32
sp|Q9XHG2|FLS_MALDO Flavonol synthase (FLS) gi|4588916|gb|AAD262...   138  7e-32

>sp|Q9M547|FLS_EUSGR Flavonol synthase (FLS) gi|7578883|gb|AAF64168.1|AF240764_1
           flavonol synthase [Eustoma grandiflorum]
          Length = 334

 Score =  144 bits (364), Expect = 7e-34
 Identities = 63/80 (78%), Positives = 74/80 (91%)
 Frame = -3

Query: 625 LVIHIGDQMEILSNGKYKAVLHRTTVNKEETRMSWPVFIEPQGDHEVGPHSKLVNQDNPP 446
           L++HIGDQ+EI+SNGKYK+V HRTTVNKE+TRMSWPVF+EP  DHEVGP  KLVN++NP 
Sbjct: 255 LIVHIGDQVEIMSNGKYKSVYHRTTVNKEKTRMSWPVFLEPPPDHEVGPIPKLVNEENPA 314

Query: 445 KYKTKKYKDYAYCKLNKIPQ 386
           K+KTKKYKDYAYCKLNK+PQ
Sbjct: 315 KFKTKKYKDYAYCKLNKLPQ 334

>sp|Q07512|FLS_PETHY Flavonol synthase (FLS) gi|421946|pir||S33510 flavonol synthase -
           garden petunia gi|311658|emb|CAA80264.1| flavonol
           synthase [Petunia x hybrida]
          Length = 348

 Score =  140 bits (353), Expect = 1e-32
 Identities = 60/80 (75%), Positives = 73/80 (91%)
 Frame = -3

Query: 625 LVIHIGDQMEILSNGKYKAVLHRTTVNKEETRMSWPVFIEPQGDHEVGPHSKLVNQDNPP 446
           L++HIGDQ+EILSNGKYK+V HRTTVNK++TRMSWPVF+EP  +HEVGP  KL+++ NPP
Sbjct: 269 LIVHIGDQVEILSNGKYKSVYHRTTVNKDKTRMSWPVFLEPPSEHEVGPIPKLLSEANPP 328

Query: 445 KYKTKKYKDYAYCKLNKIPQ 386
           K+KTKKYKDY YCKLNK+PQ
Sbjct: 329 KFKTKKYKDYVYCKLNKLPQ 348

>sp|Q41452|FLS_SOLTU Flavonol synthase (FLS) gi|7433193|pir||T07373 flavonol synthase -
           potato gi|1039357|emb|CAA63092.1| flavonol synthase
           [Solanum tuberosum]
          Length = 349

 Score =  139 bits (350), Expect = 3e-32
 Identities = 59/80 (73%), Positives = 71/80 (88%)
 Frame = -3

Query: 625 LVIHIGDQMEILSNGKYKAVLHRTTVNKEETRMSWPVFIEPQGDHEVGPHSKLVNQDNPP 446
           +++HIGDQ+EILSNGKYK+V HRTTVNK +TRMSWPVF+EP  +HEVGP   L+N+ NPP
Sbjct: 270 IIVHIGDQVEILSNGKYKSVYHRTTVNKYKTRMSWPVFLEPSSEHEVGPIPNLINEANPP 329

Query: 445 KYKTKKYKDYAYCKLNKIPQ 386
           K+KTKKYKDY YCKLNK+PQ
Sbjct: 330 KFKTKKYKDYVYCKLNKLPQ 349

>sp|Q9ZWQ9|FLS_CITUN Flavonol synthase (FLS) (CitFLS) gi|4126403|dbj|BAA36554.1|
           flavonol synthase [Citrus unshiu]
          Length = 335

 Score =  138 bits (347), Expect = 7e-32
 Identities = 62/80 (77%), Positives = 72/80 (89%)
 Frame = -3

Query: 625 LVIHIGDQMEILSNGKYKAVLHRTTVNKEETRMSWPVFIEPQGDHEVGPHSKLVNQDNPP 446
           LVIHIGDQ+EILSNGKYKAVLHRTTVNK++TRMSWPVF+EP  D  VGP  +LV+ +NPP
Sbjct: 256 LVIHIGDQIEILSNGKYKAVLHRTTVNKDKTRMSWPVFLEPPADTVVGPLPQLVDDENPP 315

Query: 445 KYKTKKYKDYAYCKLNKIPQ 386
           KYK KK+KDY+YCKLNK+PQ
Sbjct: 316 KYKAKKFKDYSYCKLNKLPQ 335

>sp|Q9XHG2|FLS_MALDO Flavonol synthase (FLS) gi|4588916|gb|AAD26261.1|AF119095_1
           flavonol synthase [Malus x domestica]
          Length = 337

 Score =  138 bits (347), Expect = 7e-32
 Identities = 61/80 (76%), Positives = 70/80 (87%)
 Frame = -3

Query: 625 LVIHIGDQMEILSNGKYKAVLHRTTVNKEETRMSWPVFIEPQGDHEVGPHSKLVNQDNPP 446
           LVIHIGDQMEI+SNGKY +VLHRTTVNK++TR+SWPVF+EP  DH VGPH +LVN  N P
Sbjct: 258 LVIHIGDQMEIMSNGKYTSVLHRTTVNKDKTRISWPVFLEPPADHVVGPHPQLVNAVNQP 317

Query: 445 KYKTKKYKDYAYCKLNKIPQ 386
           KYKTKKY DY YCK+NK+PQ
Sbjct: 318 KYKTKKYGDYVYCKINKLPQ 337

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 535,624,227
Number of Sequences: 1393205
Number of extensions: 11515822
Number of successful extensions: 29354
Number of sequences better than 10.0: 560
Number of HSP's better than 10.0 without gapping: 28262
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 29295
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 25586195130
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MF097f06_f BP033360 1 525 2 MFBL023f02_f BP042416 2 365 3 MPD056h09_f AV773786 31 387 4 MF023f03_f BP029490 104 635

---