>KMC011747A_C01 KMC011747A_c01
gtggaatggggaaaggtcagaacttgtattgaagATGAAAATCATTAATCATATCATGTG
AAAGCAATCTCATCACTATCCAGCTTCAAAAAAAAAAGAAAAACTCATCACTATCAGAAT
TTCTATTCTGAAAGTCTAGATGGAGTCTATATGCAATCAAATCTAAACTAGTCACTTGTT
TTCTGACATGAACTTCCACCATAATTGGAAAGGCTGGGAATCAATTAAGAGAGATTTGAA
AGCACTTATCAAACTGGGAAACTCAGTTACTTCAAAGGTAGAATCGATTTCACCCATGCT
TTCCATGGAGCATCCTGTTGCTGCTCAACCCAAGACAAGAAACAGCTATCCAACAAGCAG
AAGAAGTTGACATAAAGGAGCTGATACCTTACAGGTATATACCGAAAATTCGCAACCTGA
ACAACTGGCCATATACCTCCTTCTAAGATCAAGGCTGGGAGAAAATCCCTCTTCACATCC
TCTTTTATTTGAGGAACACTCTTTCCAGTAGAAAAACCCATGTAAGTAAAGAACACAAGT
AGATCCAATGGCCCAAAGAGAAACCCATCAGCCCCAACTTTAGCAGCAACGAAGCGGAAG
GAATGGGGTTTAAGCATCAGTCTCAATCTTATAAACCGATCCAAACCTTCATAC

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC011747A_C01 KMC011747A_c01
         (654 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_189100.1| unknown protein; protein id: At3g24570.1 [Arabi...   195  4e-49
dbj|BAB78679.1| P0452F10.16 [Oryza sativa (japonica cultivar-gro...   186  2e-46
gb|EAA34618.1| hypothetical protein [Neurospora crassa]                65  6e-10
ref|NP_593696.1| putative peroxisomal membrane protein [Schizosa...    62  9e-09
ref|NP_651944.1| CG11077-PA [Drosophila melanogaster] gi|7304363...    59  6e-08

>ref|NP_189100.1| unknown protein; protein id: At3g24570.1 [Arabidopsis thaliana]
           gi|9294052|dbj|BAB02009.1|
           gene_id:MOB24.15~ref|NP_013352.1~similar to unknown
           protein [Arabidopsis thaliana]
          Length = 235

 Score =  195 bits (496), Expect = 4e-49
 Identities = 90/129 (69%), Positives = 107/129 (82%), Gaps = 1/129 (0%)
 Frame = -2

Query: 653 YEGLDRFIRLRLMLKPHSFRFVAAKVGADGFLFGPLDLLVFFTYMGFSTGKSVPQIKEDV 474
           YEGLD+FI+L+L   P S RFVAAKV  DG +FGP+DLLVFFTYMGF+TGK+  ++KE +
Sbjct: 99  YEGLDKFIKLKLRYVPKSTRFVAAKVAMDGLIFGPVDLLVFFTYMGFATGKNTAEVKEGL 158

Query: 473 KRDFLPALILEGGIWPVVQVANFRYIPVRYQLLYVNFFCLLDSCFLSWVEQQQDAPWKAW 294
           KRDFLPAL LEGG WP++Q+ANFRY+PV+YQLLYVN FCL+DS FLSWVEQQ+DA WK W
Sbjct: 159 KRDFLPALALEGGAWPLLQIANFRYVPVQYQLLYVNIFCLVDSAFLSWVEQQKDAAWKQW 218

Query: 293 -VKSILPLK 270
              S  PLK
Sbjct: 219 FTSSFQPLK 227

>dbj|BAB78679.1| P0452F10.16 [Oryza sativa (japonica cultivar-group)]
          Length = 222

 Score =  186 bits (473), Expect = 2e-46
 Identities = 85/123 (69%), Positives = 104/123 (84%)
 Frame = -2

Query: 653 YEGLDRFIRLRLMLKPHSFRFVAAKVGADGFLFGPLDLLVFFTYMGFSTGKSVPQIKEDV 474
           YE LDRFI  R   +P +F+FVA+KV ADG LFGP+DLL+FF+Y+G ++G+SV Q+K+DV
Sbjct: 91  YEYLDRFILRRY--QPKTFKFVASKVAADGLLFGPVDLLLFFSYVGLASGRSVEQVKDDV 148

Query: 473 KRDFLPALILEGGIWPVVQVANFRYIPVRYQLLYVNFFCLLDSCFLSWVEQQQDAPWKAW 294
           KRDF+PAL+L G IWP VQ+ANFR+IPVRYQLLYVN FCLLDSCFLSW++QQ DAPWK W
Sbjct: 149 KRDFIPALVLGGTIWPAVQIANFRFIPVRYQLLYVNLFCLLDSCFLSWIDQQGDAPWKQW 208

Query: 293 VKS 285
             S
Sbjct: 209 FTS 211

>gb|EAA34618.1| hypothetical protein [Neurospora crassa]
          Length = 190

 Score = 65.5 bits (158), Expect = 6e-10
 Identities = 33/107 (30%), Positives = 62/107 (57%)
 Frame = -2

Query: 638 RFIRLRLMLKPHSFRFVAAKVGADGFLFGPLDLLVFFTYMGFSTGKSVPQIKEDVKRDFL 459
           RF++ R+++   + + + A+V AD  LF P  + +F   M    G  V   KE +++++ 
Sbjct: 69  RFLQKRVVVPGSTNKTILARVAADQGLFAPTFIGIFLGSMAVLEGTDV---KEKLQKNYW 125

Query: 458 PALILEGGIWPVVQVANFRYIPVRYQLLYVNFFCLLDSCFLSWVEQQ 318
            AL     +WP VQ+ NF+ +P+ +++L+VN   +  +C+LSW+  Q
Sbjct: 126 EALSTNWMVWPFVQMVNFKVVPLDHRVLFVNVISIGWNCYLSWLNGQ 172

>ref|NP_593696.1| putative peroxisomal membrane protein [Schizosaccharomyces pombe]
           gi|1723430|sp|Q10244|YD1E_SCHPO Hypothetical protein
           C4G9.14 in chromosome I gi|7492794|pir||T38873 probable
           peroxisoaml membrane protein - fission yeast
           (Schizosaccharomyces pombe) gi|1217656|emb|CAA93564.1|
           putative peroxisomal membrane protein
           [Schizosaccharomyces pombe]
          Length = 221

 Score = 61.6 bits (148), Expect = 9e-09
 Identities = 27/89 (30%), Positives = 51/89 (56%)
 Frame = -2

Query: 599 FRFVAAKVGADGFLFGPLDLLVFFTYMGFSTGKSVPQIKEDVKRDFLPALILEGGIWPVV 420
           F  +  +V  D F+F PL ++ FF +MG +  KS  ++K   ++ + P L     +WP V
Sbjct: 123 FIAIVLRVALDQFIFAPLGIVFFFLFMGITECKSYERLKSYFRKHYWPTLKANYILWPAV 182

Query: 419 QVANFRYIPVRYQLLYVNFFCLLDSCFLS 333
           Q+ NF ++P+  Q+++ N   ++ + +LS
Sbjct: 183 QLFNFTFVPLVLQVIFANAVSMVWTAYLS 211

>ref|NP_651944.1| CG11077-PA [Drosophila melanogaster] gi|7304363|gb|AAF59393.1|
           CG11077-PA [Drosophila melanogaster]
           gi|17946125|gb|AAL49104.1| RE55125p [Drosophila
           melanogaster]
          Length = 168

 Score = 58.9 bits (141), Expect = 6e-08
 Identities = 29/103 (28%), Positives = 52/103 (50%)
 Frame = -2

Query: 635 FIRLRLMLKPHSFRFVAAKVGADGFLFGPLDLLVFFTYMGFSTGKSVPQIKEDVKRDFLP 456
           F+  R+       R    K+  D  LF P   +     +  S G+ + +I++ +   +L 
Sbjct: 63  FLESRVPKTYSPMRRGVTKMLVDQTLFAPPFTMAMSFLVPLSNGEPIDRIRQRILDSYLS 122

Query: 455 ALILEGGIWPVVQVANFRYIPVRYQLLYVNFFCLLDSCFLSWV 327
            L+    +WP  Q+ NFR++P+ YQ+LY  F  L+ +C+LS +
Sbjct: 123 ILVRNYMLWPAAQMLNFRFVPLGYQVLYAQFIALVWNCYLSMI 165

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 552,559,024
Number of Sequences: 1393205
Number of extensions: 11834751
Number of successful extensions: 38050
Number of sequences better than 10.0: 58
Number of HSP's better than 10.0 without gapping: 36999
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 38034
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 28144814643
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MPD038d02_f AV772594 1 513 2 SPD058b03_f BP048590 35 529 3 MF023a06_f BP029461 36 507 4 SPD062d10_f BP048940 52 563 5 SPD042g12_f BP047379 88 657

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