KMC011627A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC011627A_C01 KMC011627A_c01
gagtgaaacttattcatctttaattgaaagggacaattaattacgatacacatgcatctg
gttggagataaagtaggaaaTGGAAACACCATCATATTCATAACGCATTTTATATACATA
TGAATTAAAAAAAAAAAAATCCTGTGATCTAGACTCATGAACAACAATGGATCACACTCA
ACTTCATCAAACTTCAAACTTCTTCAAATAACATCTCTCTATTCTGTGTTTTCTCCACTC
TCACTTGATGCTGATCTTTTGTTCACATATGCAGTTGAAGGCTTTTATTTGCAATAATAA
GTTTCCTAAGTCCTTGATGATAGGAAAGCAAAAAAAGGAAAACAGAAAACATGCATGCAC
ATATATTTTGTGTAAAACAAAGAGCCATAATATTGTAGCTCAGCAATATCTTCTAAGAAA
GAGGATTATTAAAACAGGTGGTAGTGGAGGCAATTTGCCAAACTTAGGGTGATGGAAAAA
CTTGGTGGGAGGCAGTGGAGGTAATTTCCCAAGACTGGGGTGATGGAAGAAGCGTTGGTG
AGGTAGTGGAGGCAATTTTCCATACGTGGGGTGGTGGAAAAACTTTATGGGAGGTA


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC011627A_C01 KMC011627A_c01
         (596 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAF28388.1|AF151217_1 proline-rich protein [Arabidopsis thali...    53  3e-06
gb|AAF64551.1|AF110988_1 proline-rich protein 4 [Arabidopsis tha...    53  3e-06
ref|NP_195588.1| extensin - like protein; protein id: At4g38770....    53  3e-06
sp|Q38913|EXT1_ARATH Extensin 1 precursor (AtExt1) (AtExt4) gi|2...    40  0.023
pir||S71227 extensin 1 - Arabidopsis thaliana gi|1167961|gb|AAA8...    40  0.023

>gb|AAF28388.1|AF151217_1 proline-rich protein [Arabidopsis thaliana]
           gi|21592385|gb|AAM64336.1| extensin-like protein
           [Arabidopsis thaliana]
          Length = 448

 Score = 52.8 bits (125), Expect = 3e-06
 Identities = 28/57 (49%), Positives = 31/57 (54%), Gaps = 4/57 (7%)
 Frame = -3

Query: 594 PPIKFFHHPTY----GKLPPLPHQRFFHHPSLGKLPPLPPTKFFHHPKFGKLPPLPP 436
           PP+  +  P         PPLP       P L K PPLPP K+ HHPKFGK PPLPP
Sbjct: 396 PPVPVYKPPVVVIPKKPCPPLPQL-----PPLPKFPPLPP-KYIHHPKFGKWPPLPP 446

>gb|AAF64551.1|AF110988_1 proline-rich protein 4 [Arabidopsis thaliana]
          Length = 448

 Score = 52.8 bits (125), Expect = 3e-06
 Identities = 28/57 (49%), Positives = 31/57 (54%), Gaps = 4/57 (7%)
 Frame = -3

Query: 594 PPIKFFHHPTY----GKLPPLPHQRFFHHPSLGKLPPLPPTKFFHHPKFGKLPPLPP 436
           PP+  +  P         PPLP       P L K PPLPP K+ HHPKFGK PPLPP
Sbjct: 396 PPVPVYKPPVVVIPKKPCPPLPQL-----PPLPKFPPLPP-KYIHHPKFGKWPPLPP 446

>ref|NP_195588.1| extensin - like protein; protein id: At4g38770.1, supported by
           cDNA: gi_15809889, supported by cDNA: gi_20260185,
           supported by cDNA: gi_6782441 [Arabidopsis thaliana]
           gi|7488231|pir||T06076 proline-rich protein GPP1 homolog
           T9A14.50 - Arabidopsis thaliana
           gi|4490329|emb|CAB38611.1| extensin-like protein
           [Arabidopsis thaliana] gi|7270860|emb|CAB80540.1|
           extensin-like protein [Arabidopsis thaliana]
           gi|15809890|gb|AAL06873.1| AT4g38770/T9A14_50
           [Arabidopsis thaliana] gi|20260186|gb|AAM12991.1|
           extensin-like protein [Arabidopsis thaliana]
           gi|24030361|gb|AAN41344.1| putative extensin protein
           [Arabidopsis thaliana] gi|24899753|gb|AAN65091.1|
           extensin-like protein [Arabidopsis thaliana]
          Length = 448

 Score = 52.8 bits (125), Expect = 3e-06
 Identities = 28/57 (49%), Positives = 31/57 (54%), Gaps = 4/57 (7%)
 Frame = -3

Query: 594 PPIKFFHHPTY----GKLPPLPHQRFFHHPSLGKLPPLPPTKFFHHPKFGKLPPLPP 436
           PP+  +  P         PPLP       P L K PPLPP K+ HHPKFGK PPLPP
Sbjct: 396 PPVPVYKPPVVVIPKKPCPPLPQL-----PPLPKFPPLPP-KYIHHPKFGKWPPLPP 446

>sp|Q38913|EXT1_ARATH Extensin 1 precursor (AtExt1) (AtExt4) gi|25307716|pir||B96798
           extensin [imported] - Arabidopsis thaliana
           gi|2829922|gb|AAC00630.1| extensin [Arabidopsis
           thaliana]
          Length = 373

 Score = 40.0 bits (92), Expect = 0.023
 Identities = 22/54 (40%), Positives = 30/54 (54%)
 Frame = -3

Query: 594 PPIKFFHHPTYGKLPPLPHQRFFHHPSLGKLPPLPPTKFFHHPKFGKLPPLPPV 433
           PP+K++  P   K PP P  + +  P + K PP PP K++  P   K PP PPV
Sbjct: 196 PPVKYYSPPPVYKSPP-PPVKHYSPPPVYKSPP-PPVKYYSPPPVYKSPP-PPV 246

 Score = 40.0 bits (92), Expect = 0.023
 Identities = 22/54 (40%), Positives = 30/54 (54%)
 Frame = -3

Query: 594 PPIKFFHHPTYGKLPPLPHQRFFHHPSLGKLPPLPPTKFFHHPKFGKLPPLPPV 433
           PP+K++  P   K PP P  + +  P + K PP PP K++  P   K PP PPV
Sbjct: 164 PPVKYYSPPPVYKSPP-PPVKHYSPPPVYKSPP-PPVKYYSPPPVYKSPP-PPV 214

 Score = 38.5 bits (88), Expect = 0.066
 Identities = 22/54 (40%), Positives = 29/54 (52%)
 Frame = -3

Query: 594 PPIKFFHHPTYGKLPPLPHQRFFHHPSLGKLPPLPPTKFFHHPKFGKLPPLPPV 433
           PP+K +  P   K PP P  + +  P + K PP PP K++  P   K PP PPV
Sbjct: 44  PPVKHYSPPPVYKSPP-PPVKHYSPPPVYKSPP-PPVKYYSPPPVYKSPP-PPV 94

 Score = 38.5 bits (88), Expect = 0.066
 Identities = 22/54 (40%), Positives = 29/54 (52%)
 Frame = -3

Query: 594 PPIKFFHHPTYGKLPPLPHQRFFHHPSLGKLPPLPPTKFFHHPKFGKLPPLPPV 433
           PP+K +  P   K PP P  + +  P + K PP PP K++  P   K PP PPV
Sbjct: 132 PPVKHYSPPPVYKSPP-PPVKHYSPPPVYKSPP-PPVKYYSPPPVYKSPP-PPV 182

 Score = 37.0 bits (84), Expect = 0.19
 Identities = 22/54 (40%), Positives = 28/54 (51%)
 Frame = -3

Query: 594 PPIKFFHHPTYGKLPPLPHQRFFHHPSLGKLPPLPPTKFFHHPKFGKLPPLPPV 433
           PP+K +  P   K PP P  + +  P + K PP PP K +  P   K PP PPV
Sbjct: 28  PPVKHYSPPPVYKSPP-PPVKHYSPPPVYKSPP-PPVKHYSPPPVYKSPP-PPV 78

 Score = 37.0 bits (84), Expect = 0.19
 Identities = 22/54 (40%), Positives = 28/54 (51%)
 Frame = -3

Query: 594 PPIKFFHHPTYGKLPPLPHQRFFHHPSLGKLPPLPPTKFFHHPKFGKLPPLPPV 433
           PP+K +  P   K PP P  + +  P + K PP PP K +  P   K PP PPV
Sbjct: 100 PPVKHYSPPPVYKSPP-PPVKHYSPPPVYKSPP-PPVKHYSPPPVYKSPP-PPV 150

 Score = 35.0 bits (79), Expect = 0.73
 Identities = 22/61 (36%), Positives = 29/61 (47%), Gaps = 7/61 (11%)
 Frame = -3

Query: 594 PPIKFFHHPTYGKLPPLPHQRFFHHPSLGKLPPLP-------PTKFFHHPKFGKLPPLPP 436
           PP+K +  P   K PP P  +++  P + K PP P       P K +  P   K PP PP
Sbjct: 60  PPVKHYSPPPVYKSPP-PPVKYYSPPPVYKSPPPPVYKSPPPPVKHYSPPPVYKSPP-PP 117

Query: 435 V 433
           V
Sbjct: 118 V 118

>pir||S71227 extensin 1 - Arabidopsis thaliana gi|1167961|gb|AAA85899.1|
           extensin
          Length = 284

 Score = 40.0 bits (92), Expect = 0.023
 Identities = 22/54 (40%), Positives = 30/54 (54%)
 Frame = -3

Query: 594 PPIKFFHHPTYGKLPPLPHQRFFHHPSLGKLPPLPPTKFFHHPKFGKLPPLPPV 433
           PP+K++  P   K PP P  + +  P + K PP PP K++  P   K PP PPV
Sbjct: 108 PPVKYYSPPPVYKSPP-PPVKHYSPPPVYKSPP-PPVKYYSPPPVYKSPP-PPV 158

 Score = 39.7 bits (91), Expect = 0.030
 Identities = 23/54 (42%), Positives = 29/54 (53%)
 Frame = -3

Query: 594 PPIKFFHHPTYGKLPPLPHQRFFHHPSLGKLPPLPPTKFFHHPKFGKLPPLPPV 433
           PP+K +  P   K PP P  R++  P + K PP PP K +  P   K PP PPV
Sbjct: 60  PPVKHYSPPPVYKSPP-PPVRYYSPPPVYKSPP-PPVKHYSPPPVYKSPP-PPV 110

 Score = 38.9 bits (89), Expect = 0.050
 Identities = 21/54 (38%), Positives = 30/54 (54%)
 Frame = -3

Query: 594 PPIKFFHHPTYGKLPPLPHQRFFHHPSLGKLPPLPPTKFFHHPKFGKLPPLPPV 433
           PP++++  P   K PP P  + +  P + K PP PP K++  P   K PP PPV
Sbjct: 76  PPVRYYSPPPVYKSPP-PPVKHYSPPPVYKSPP-PPVKYYSPPPVYKSPP-PPV 126

 Score = 37.0 bits (84), Expect = 0.19
 Identities = 22/54 (40%), Positives = 28/54 (51%)
 Frame = -3

Query: 594 PPIKFFHHPTYGKLPPLPHQRFFHHPSLGKLPPLPPTKFFHHPKFGKLPPLPPV 433
           PP+K +  P   K PP P  + +  P + K PP PP K +  P   K PP PPV
Sbjct: 28  PPVKHYSPPPVYKSPP-PPVKHYSPPPVYKSPP-PPVKHYSPPPVYKSPP-PPV 78

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 550,515,817
Number of Sequences: 1393205
Number of extensions: 13183993
Number of successful extensions: 38571
Number of sequences better than 10.0: 105
Number of HSP's better than 10.0 without gapping: 34000
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 37820
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 23140425222
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 MF033a07_f BP030005 1 537
2 MPD031e11_f AV772134 96 600
3 MF014a11_f BP028959 96 475




Lotus japonicus
Kazusa DNA Research Institute