>KMC011378A_C01 KMC011378A_c01
ttaaagcagagactgaatgacattaaaatggatattgaacaaaaaatcaatgacatgaat
ggatgagtgtagcccccctcCCCCCACAAAATTAAAGTCACATATATAGAAAGTGATCTT
CAAAAACATATTCTAAATTCTTTAAATCATTACAAATCCATAACAAAACCCCATCTGAAA
GGGGTTCACCAAAAAAATAGCACATGGCTATTGGCTAGCTAACATTTTCCTTATGATCAA
TAAACGCTGAAACAATCAAACCAACAATATTACTCAATTCTTCCAAAAGCAGAGCTTATA
TTATTACCTTTCACTGCTTCTGGCCACTATACATGGACTCCACCTTCCCATCATTCCCTC
CCAGCAACAAATACTTATCTTTCCTGGTCAAGCCAGTGCATTCAAACCCAAGAACATCCC
CCAACACCTTCTGGACATGATTCGCCACCTCAATCGACGACTTCCCTCCGGCCTTACACG
ACATCTCCTCCGGCAACCGGTCAAGGAAGGTAGCCTCATAAACCGGCCTAGGATTCATGA
AGAAGAAGTAAGGGTCCCAGAACTTGACACCACGCACGGTGGTCCCGTAGAACATGCTCT
GCTTACAGTCAACGGCGACGGGGACAATCCGGTCACTGAGCTCTGCAAACAAA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC011378A_C01 KMC011378A_c01
         (653 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_191950.1| putative protein; protein id: At4g00400.1 [Arab...   191  6e-48
gb|AAL32544.1| Unknown protein [Arabidopsis thaliana] gi|2025989...   189  3e-47
ref|NP_171667.1| hypothetical protein; protein id: At1g01610.1, ...   189  3e-47
ref|NP_181346.1| unknown protein; protein id: At2g38110.1, suppo...   151  6e-36
ref|NP_563768.1| expressed protein; protein id: At1g06520.1, sup...   136  3e-31

>ref|NP_191950.1| putative protein; protein id: At4g00400.1 [Arabidopsis thaliana]
           gi|7485267|pir||T01531 hypothetical protein A_IG005I10.4
           - Arabidopsis thaliana gi|2252827|gb|AAB62826.1|
           A_IG005I10.4 gene product [Arabidopsis thaliana]
           gi|6049869|gb|AAF02784.1|AF195115_4 F5I10.4 gene product
           [Arabidopsis thaliana] gi|7267127|emb|CAB80798.1|
           putative protein [Arabidopsis thaliana]
          Length = 289

 Score =  191 bits (486), Expect = 6e-48
 Identities = 92/112 (82%), Positives = 101/112 (90%)
 Frame = -2

Query: 652 LFAELSDRIVPVAVDCKQSMFYGTTVRGVKFWDPYFFFMNPRPVYEATFLDRLPEEMSCK 473
           LFAELSDRIVPVA++CKQ MF GTTVRGVKFWDPYFFFMNPRP YEATFLDRLPEEM+  
Sbjct: 178 LFAELSDRIVPVAMNCKQGMFNGTTVRGVKFWDPYFFFMNPRPSYEATFLDRLPEEMTVN 237

Query: 472 AGGKSSIEVANHVQKVLGDVLGFECTGLTRKDKYLLLGGNDGKVESMYSGQK 317
            GGK+ IEVAN+VQKV+G VLGFECT LTRKDKYLLLGGNDGKVES+ + +K
Sbjct: 238 GGGKTPIEVANYVQKVIGAVLGFECTELTRKDKYLLLGGNDGKVESINNTKK 289

>gb|AAL32544.1| Unknown protein [Arabidopsis thaliana] gi|20259892|gb|AAM13293.1|
           unknown protein [Arabidopsis thaliana]
          Length = 503

 Score =  189 bits (480), Expect = 3e-47
 Identities = 90/107 (84%), Positives = 97/107 (90%)
 Frame = -2

Query: 652 LFAELSDRIVPVAVDCKQSMFYGTTVRGVKFWDPYFFFMNPRPVYEATFLDRLPEEMSCK 473
           LFAELSDRIVPVA++CKQ MF GTTVRGVKFWDPYFFFMNPRP YEATFLDRLPEEM+  
Sbjct: 390 LFAELSDRIVPVAMNCKQGMFNGTTVRGVKFWDPYFFFMNPRPSYEATFLDRLPEEMTVN 449

Query: 472 AGGKSSIEVANHVQKVLGDVLGFECTGLTRKDKYLLLGGNDGKVESM 332
            GGK+  EVAN+VQKV+G VLGFECT LTRKDKYLLLGGNDGKVES+
Sbjct: 450 GGGKTPFEVANYVQKVIGGVLGFECTELTRKDKYLLLGGNDGKVESI 496

>ref|NP_171667.1| hypothetical protein; protein id: At1g01610.1, supported by cDNA:
           gi_17064779, supported by cDNA: gi_20259891 [Arabidopsis
           thaliana] gi|25372669|pir||H86146 hypothetical protein
           F22L4.15 - Arabidopsis thaliana
           gi|8920597|gb|AAF81319.1|AC061957_15 Contains similarity
           to a hypothetical protein F16M14.4 gi|7485589 from
           Arabidopsis thaliana BAC F16M14 gb|T01243
          Length = 503

 Score =  189 bits (480), Expect = 3e-47
 Identities = 90/107 (84%), Positives = 97/107 (90%)
 Frame = -2

Query: 652 LFAELSDRIVPVAVDCKQSMFYGTTVRGVKFWDPYFFFMNPRPVYEATFLDRLPEEMSCK 473
           LFAELSDRIVPVA++CKQ MF GTTVRGVKFWDPYFFFMNPRP YEATFLDRLPEEM+  
Sbjct: 390 LFAELSDRIVPVAMNCKQGMFNGTTVRGVKFWDPYFFFMNPRPSYEATFLDRLPEEMTVN 449

Query: 472 AGGKSSIEVANHVQKVLGDVLGFECTGLTRKDKYLLLGGNDGKVESM 332
            GGK+  EVAN+VQKV+G VLGFECT LTRKDKYLLLGGNDGKVES+
Sbjct: 450 GGGKTPFEVANYVQKVIGGVLGFECTELTRKDKYLLLGGNDGKVESI 496

>ref|NP_181346.1| unknown protein; protein id: At2g38110.1, supported by cDNA:
           gi_17065289 [Arabidopsis thaliana]
           gi|7485589|pir||T01243 hypothetical protein At2g38110
           [imported] - Arabidopsis thaliana
           gi|3335359|gb|AAC27160.1| unknown protein [Arabidopsis
           thaliana] gi|17065290|gb|AAL32799.1| Unknown protein
           [Arabidopsis thaliana] gi|21387145|gb|AAM47976.1|
           unknown protein [Arabidopsis thaliana]
          Length = 501

 Score =  151 bits (382), Expect = 6e-36
 Identities = 72/104 (69%), Positives = 84/104 (80%)
 Frame = -2

Query: 652 LFAELSDRIVPVAVDCKQSMFYGTTVRGVKFWDPYFFFMNPRPVYEATFLDRLPEEMSCK 473
           LFAEL+DRIVPVA++ KQSMF GTT RG K  DPYF FMNPRP YE TFL ++P E++CK
Sbjct: 392 LFAELTDRIVPVAINTKQSMFNGTTTRGYKLLDPYFAFMNPRPTYEITFLKQIPAELTCK 451

Query: 472 AGGKSSIEVANHVQKVLGDVLGFECTGLTRKDKYLLLGGNDGKV 341
            GGKS IEVAN++Q+VLG  LGFECT  TRKDKY +L G DG+V
Sbjct: 452 -GGKSPIEVANYIQRVLGGTLGFECTNFTRKDKYAMLAGTDGRV 494

>ref|NP_563768.1| expressed protein; protein id: At1g06520.1, supported by cDNA:
           94974. [Arabidopsis thaliana] gi|25372666|pir||G86200
           protein F12K11.15 [imported] - Arabidopsis thaliana
           gi|6692682|gb|AAF24816.1|AC007592_9 F12K11.15
           [Arabidopsis thaliana]
          Length = 585

 Score =  136 bits (342), Expect = 3e-31
 Identities = 69/104 (66%), Positives = 80/104 (76%)
 Frame = -2

Query: 652 LFAELSDRIVPVAVDCKQSMFYGTTVRGVKFWDPYFFFMNPRPVYEATFLDRLPEEMSCK 473
           LFAEL++ IVPVAVD + SMFYGTT  G+K  DP FF MNPRPVY    L +LP+EM+C 
Sbjct: 482 LFAELTEDIVPVAVDARVSMFYGTTASGLKCLDPIFFLMNPRPVYCLEILKKLPKEMTC- 540

Query: 472 AGGKSSIEVANHVQKVLGDVLGFECTGLTRKDKYLLLGGNDGKV 341
           AGGKSS EVAN +Q  L  VLGFECT LTR+DKYL+L GN+G V
Sbjct: 541 AGGKSSFEVANFIQGELARVLGFECTNLTRRDKYLVLAGNEGIV 584

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 617,215,423
Number of Sequences: 1393205
Number of extensions: 14878752
Number of successful extensions: 48081
Number of sequences better than 10.0: 95
Number of HSP's better than 10.0 without gapping: 42716
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 47560
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 28144814643
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MF012f03_f BP028879 1 528 2 SPD002d07_f BP044158 108 541 3 SPD028d01_f BP046211 108 628 4 SPD072f08_f BP049774 108 299 5 SPD094e07_f BP051515 108 618 6 SPDL061g01_f BP055821 114 294 7 MPDL001c07_f AV776563 115 278 8 MF075d05_f BP032274 132 568 9 MWM230c03_f AV768232 133 465 10 MPD016g07_f AV771122 136 656 11 SPD047e03_f BP047747 140 637

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