Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC011009A_C01 KMC011009A_c01
(602 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||T09649 delta-1-pyrroline-5-carboxylate synthase - alfalfa g... 170 4e-46
emb|CAC82184.1| pyrroline-5-carboxylate synthetase 1 [Medicago t... 167 3e-45
gb|AAK01361.1|AF314812_1 delta 1-pyrroline-5-carboxylate synthet... 164 1e-43
sp|O04015|P5CS_ACTCH Delta 1-pyrroline-5-carboxylate synthetase ... 166 6e-43
ref|NP_181510.1| delta-1-pyrroline 5-carboxylase synthetase (P5C... 161 7e-43
>pir||T09649 delta-1-pyrroline-5-carboxylate synthase - alfalfa
gi|1419036|emb|CAA67069.1|
delta-1-pyrroline-5-carboxylate synthase [Medicago
sativa]
Length = 752
Score = 170 bits (430), Expect(2) = 4e-46
Identities = 81/97 (83%), Positives = 88/97 (90%)
Frame = -2
Query: 520 IFHSAHTDCIVAEDHEVADVFLRQVDSASVIHNASTRFCDGARFGLGAEVGTSTSRIHAR 341
++ SAHTD IV EDHEVADVFLRQVDSA+V HNASTRF DGARFGLGAEVG STSRIHAR
Sbjct: 656 LYGSAHTDSIVTEDHEVADVFLRQVDSAAVFHNASTRFSDGARFGLGAEVGISTSRIHAR 715
Query: 340 GPVGVEGLLTTRWILKGSGQVVDGDKEIVYTHRDLAT 230
GPVGV+GLLTTRW+LKGSGQVVDGDK + YTH+DL T
Sbjct: 716 GPVGVDGLLTTRWLLKGSGQVVDGDKTVTYTHKDLTT 752
Score = 36.6 bits (83), Expect(2) = 4e-46
Identities = 15/17 (88%), Positives = 17/17 (99%)
Frame = -1
Query: 563 GVTLYGGPKATSLLNIP 513
GVTLYGGPKA+SLLN+P
Sbjct: 609 GVTLYGGPKASSLLNVP 625
>emb|CAC82184.1| pyrroline-5-carboxylate synthetase 1 [Medicago truncatula]
Length = 715
Score = 167 bits (423), Expect(2) = 3e-45
Identities = 79/96 (82%), Positives = 88/96 (91%)
Frame = -2
Query: 517 FHSAHTDCIVAEDHEVADVFLRQVDSASVIHNASTRFCDGARFGLGAEVGTSTSRIHARG 338
+ SAHTD IV EDHEVA+VFLRQVDSA+V HNASTRF DGARFGLGAEVG STSRIHARG
Sbjct: 620 YGSAHTDSIVTEDHEVANVFLRQVDSAAVFHNASTRFSDGARFGLGAEVGISTSRIHARG 679
Query: 337 PVGVEGLLTTRWILKGSGQVVDGDKEIVYTHRDLAT 230
PVGV+GLLTTRW+LKG+GQVVDGDK++ YTHRD+ T
Sbjct: 680 PVGVDGLLTTRWLLKGNGQVVDGDKKVTYTHRDITT 715
Score = 36.6 bits (83), Expect(2) = 3e-45
Identities = 15/17 (88%), Positives = 17/17 (99%)
Frame = -1
Query: 563 GVTLYGGPKATSLLNIP 513
GVTLYGGPKA+SLLN+P
Sbjct: 572 GVTLYGGPKASSLLNVP 588
>gb|AAK01361.1|AF314812_1 delta 1-pyrroline-5-carboxylate synthetase B [Brassica napus]
Length = 727
Score = 164 bits (416), Expect(2) = 1e-43
Identities = 77/92 (83%), Positives = 84/92 (90%)
Frame = -2
Query: 511 SAHTDCIVAEDHEVADVFLRQVDSASVIHNASTRFCDGARFGLGAEVGTSTSRIHARGPV 332
SAHTDCIV ED EVA++FLRQVDSA+VIHNASTRFCDG RFGLGAEVG STSRIHARGPV
Sbjct: 623 SAHTDCIVTEDSEVAEIFLRQVDSAAVIHNASTRFCDGFRFGLGAEVGISTSRIHARGPV 682
Query: 331 GVEGLLTTRWILKGSGQVVDGDKEIVYTHRDL 236
GVEGLLTTRWI++G GQVVDGD + YTH+DL
Sbjct: 683 GVEGLLTTRWIMRGKGQVVDGDNGVAYTHKDL 714
Score = 33.9 bits (76), Expect(2) = 1e-43
Identities = 14/18 (77%), Positives = 16/18 (88%)
Frame = -1
Query: 563 GVTLYGGPKATSLLNIPQ 510
GVTLYGGPKA+ LNIP+
Sbjct: 573 GVTLYGGPKASGKLNIPE 590
>sp|O04015|P5CS_ACTCH Delta 1-pyrroline-5-carboxylate synthetase (P5CS) [Includes:
Glutamate 5-kinase (Gamma-glutamyl kinase) (GK);
Gamma-glutamyl phosphate reductase (GPR)
(Glutamate-5-semialdehyde dehydrogenase)
(Glutamyl-gamma-semialdehyde dehydrogenase)]
gi|1928960|gb|AAC14481.1| pyrroline-5-carboxylate
synthetase [Actinidia deliciosa]
Length = 717
Score = 166 bits (420), Expect(2) = 6e-43
Identities = 79/94 (84%), Positives = 87/94 (92%)
Frame = -2
Query: 511 SAHTDCIVAEDHEVADVFLRQVDSASVIHNASTRFCDGARFGLGAEVGTSTSRIHARGPV 332
SAHTD I+ EDHEVA++FLRQVDS+SV+HNASTRF DGARFGLGAEVG STSRIHARGPV
Sbjct: 622 SAHTDSIITEDHEVAEIFLRQVDSSSVLHNASTRFSDGARFGLGAEVGISTSRIHARGPV 681
Query: 331 GVEGLLTTRWILKGSGQVVDGDKEIVYTHRDLAT 230
GVEGLLTTRWI +GSGQVVDGDK IVYTH+DL +
Sbjct: 682 GVEGLLTTRWIARGSGQVVDGDKGIVYTHKDLTS 715
Score = 30.0 bits (66), Expect(2) = 6e-43
Identities = 11/18 (61%), Positives = 17/18 (94%)
Frame = -1
Query: 563 GVTLYGGPKATSLLNIPQ 510
GV ++GGP+A+SLL+IP+
Sbjct: 572 GVVIHGGPRASSLLHIPE 589
>ref|NP_181510.1| delta-1-pyrroline 5-carboxylase synthetase (P5C1); protein id:
At2g39800.1, supported by cDNA: gi_1532270, supported by
cDNA: gi_15983516, supported by cDNA: gi_19423915
[Arabidopsis thaliana] gi|1709534|sp|P54887|P5C1_ARATH
Delta 1-pyrroline-5-carboxylate synthetase A (P5CS A)
[Includes: Glutamate 5-kinase (Gamma-glutamyl kinase)
(GK); Gamma-glutamyl phosphate reductase (GPR)
(Glutamate-5-semialdehyde dehydrogenase)
(Glutamyl-gamma-semialdehyde dehydrogenase)]
gi|2129572|pir||S66637 delta-1-pyrroline-5 carboxylase
synthetase [imported] - Arabidopsis thaliana
gi|829100|emb|CAA60740.1| pyrroline-5-carboxylate
synthetase [Arabidopsis thaliana]
gi|870866|emb|CAA60446.1| pyrroline-5-carboxylate
synthetase A [Arabidopsis thaliana]
gi|1041248|emb|CAA61593.1| pyrroline-5-carboxylate
synthase [Arabidopsis thaliana]
gi|2642162|gb|AAB87129.1| delta-1-pyrroline
5-carboxylase synthetase (P5C1) [Arabidopsis thaliana]
gi|15983517|gb|AAL11626.1|AF424633_1 At2g39800/T5I7.10
[Arabidopsis thaliana] gi|21360477|gb|AAM47354.1|
At2g39800/T5I7.10 [Arabidopsis thaliana]
gi|23297451|gb|AAN12972.1| delta-1-pyrroline
5-carboxylase synthetase (P5C1) [Arabidopsis thaliana]
Length = 717
Score = 161 bits (408), Expect(2) = 7e-43
Identities = 76/92 (82%), Positives = 83/92 (89%)
Frame = -2
Query: 511 SAHTDCIVAEDHEVADVFLRQVDSASVIHNASTRFCDGARFGLGAEVGTSTSRIHARGPV 332
SAHTDCIV EDHEVA++FLRQVDSA+V HNASTRF DG RFGLGAEVG ST RIHARGPV
Sbjct: 622 SAHTDCIVTEDHEVAELFLRQVDSAAVFHNASTRFSDGFRFGLGAEVGVSTGRIHARGPV 681
Query: 331 GVEGLLTTRWILKGSGQVVDGDKEIVYTHRDL 236
GVEGLLTTRWI++G GQVVDGD IVYTH+D+
Sbjct: 682 GVEGLLTTRWIMRGKGQVVDGDNGIVYTHQDI 713
Score = 34.3 bits (77), Expect(2) = 7e-43
Identities = 13/18 (72%), Positives = 17/18 (94%)
Frame = -1
Query: 563 GVTLYGGPKATSLLNIPQ 510
GVTLYGGP+A+ +LNIP+
Sbjct: 572 GVTLYGGPRASKILNIPE 589
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 563,135,903
Number of Sequences: 1393205
Number of extensions: 12721648
Number of successful extensions: 33136
Number of sequences better than 10.0: 175
Number of HSP's better than 10.0 without gapping: 31766
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 33060
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 23711793746
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
