Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC009513A_C01 KMC009513A_c01
(583 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|P08927|RUBB_PEA RuBisCO subunit binding-protein beta subunit,... 288 3e-77
pir||T07733 probable chaperonin 60 beta chain precursor, chlorop... 250 8e-66
ref|NP_200461.1| RuBisCO subunit binding-protein beta subunit pr... 247 9e-65
ref|NP_187956.1| chaperonin 60 beta, putative; protein id: At3g1... 241 5e-63
dbj|BAA92724.1| ESTs C99033(E4350),C99032(E4350),D46006(S10372),... 239 1e-62
>sp|P08927|RUBB_PEA RuBisCO subunit binding-protein beta subunit, chloroplast precursor
(60 kDa chaperonin beta subunit) (CPN-60 beta)
gi|7443853|pir||T06412 probable chaperonin 60 beta chain
- garden pea chloroplast gi|806808|gb|AAA66365.1|
chaperonin precursor [Pisum sativum]
Length = 595
Score = 288 bits (738), Expect = 3e-77
Identities = 154/177 (87%), Positives = 161/177 (90%)
Frame = +2
Query: 53 STAAGSCNLSSSASISAFPFAGGARRSSSSSSNSVVLSRKTRNLKVSAMAKELHFNKDGS 232
S SCNLSSSA+IS+FP A G R ++N VVL RK RN+KVSAMAKELHFNKDGS
Sbjct: 6 SATTSSCNLSSSAAISSFPLAAGKR-----NANKVVLPRKNRNVKVSAMAKELHFNKDGS 60
Query: 233 AIKRLQAGVNKLADLVGVTLGPKGRNVVLESKYGSPKIVNDGVTVAKEVELEDPVENIGA 412
AIK+LQ GVNKLADLVGVTLGPKGRNVVLESKYGSPKIVNDGVTVAKEVELEDPVENIGA
Sbjct: 61 AIKKLQNGVNKLADLVGVTLGPKGRNVVLESKYGSPKIVNDGVTVAKEVELEDPVENIGA 120
Query: 413 KLVRQAAAKTNDLAGDGTTTSVVLAQGLIAEGVKVVAAGANPVLITRGIEKTTKALV 583
KLVRQAAAKTNDLAGDGTTTSVVLAQGLIAEGVKVVAAGANPVLITRGIEKT+KALV
Sbjct: 121 KLVRQAAAKTNDLAGDGTTTSVVLAQGLIAEGVKVVAAGANPVLITRGIEKTSKALV 177
>pir||T07733 probable chaperonin 60 beta chain precursor, chloroplast (clone
potbchap1) - potato gi|1762130|gb|AAB39827.1|
chaperonin-60 beta subunit
Length = 599
Score = 250 bits (639), Expect = 8e-66
Identities = 139/179 (77%), Positives = 152/179 (84%)
Frame = +2
Query: 47 MASTAAGSCNLSSSASISAFPFAGGARRSSSSSSNSVVLSRKTRNLKVSAMAKELHFNKD 226
+A+ + S LSS A+IS+ F G++R+ V RK+R + A AKELHFNKD
Sbjct: 14 LAAPSTSSNKLSSVANISSTSF--GSKRN--------VALRKSRRPTILAAAKELHFNKD 63
Query: 227 GSAIKRLQAGVNKLADLVGVTLGPKGRNVVLESKYGSPKIVNDGVTVAKEVELEDPVENI 406
GSAIK+LQ GVNKLADLVGVTLGPKGRNVVLESKYG+PKIVNDGVTVA+EVELEDPVENI
Sbjct: 64 GSAIKKLQNGVNKLADLVGVTLGPKGRNVVLESKYGAPKIVNDGVTVAREVELEDPVENI 123
Query: 407 GAKLVRQAAAKTNDLAGDGTTTSVVLAQGLIAEGVKVVAAGANPVLITRGIEKTTKALV 583
GAKLVRQAAAKTNDLAGDGTTTSVVLAQGLIAEGVKVVAAGANPVLITRGIEKT KALV
Sbjct: 124 GAKLVRQAAAKTNDLAGDGTTTSVVLAQGLIAEGVKVVAAGANPVLITRGIEKTAKALV 182
>ref|NP_200461.1| RuBisCO subunit binding-protein beta subunit precursor; chaperonin,
60 kDa; protein id: At5g56500.1 [Arabidopsis thaliana]
Length = 596
Score = 247 bits (630), Expect = 9e-65
Identities = 137/180 (76%), Positives = 153/180 (84%), Gaps = 1/180 (0%)
Frame = +2
Query: 47 MASTAAGSCNLSSSASISAFPFAGGARRSSSSSSNSV-VLSRKTRNLKVSAMAKELHFNK 223
MAST + + ++ SS + + + SSSS + + RK R K+ A AK+LHFNK
Sbjct: 1 MASTFSATSSMGSSLAPPSNRLSSFVSISSSSFGRTQSIAQRKARFPKIYA-AKQLHFNK 59
Query: 224 DGSAIKRLQAGVNKLADLVGVTLGPKGRNVVLESKYGSPKIVNDGVTVAKEVELEDPVEN 403
DG+AIK+LQAGVNKLADLVGVTLGPKGRNVVLESKYGSP+IVNDGVTVA+EVELEDPVEN
Sbjct: 60 DGTAIKKLQAGVNKLADLVGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREVELEDPVEN 119
Query: 404 IGAKLVRQAAAKTNDLAGDGTTTSVVLAQGLIAEGVKVVAAGANPVLITRGIEKTTKALV 583
IGAKLVRQAA+KTNDLAGDGTTTSVVLAQGLIAEGVKVVAAGANPVLITRGIEKTTKALV
Sbjct: 120 IGAKLVRQAASKTNDLAGDGTTTSVVLAQGLIAEGVKVVAAGANPVLITRGIEKTTKALV 179
>ref|NP_187956.1| chaperonin 60 beta, putative; protein id: At3g13470.1 [Arabidopsis
thaliana] gi|9280299|dbj|BAB01754.1| GloEL protein;
chaperonin, 60 kDa [Arabidopsis thaliana]
Length = 596
Score = 241 bits (615), Expect = 5e-63
Identities = 135/180 (75%), Positives = 147/180 (81%), Gaps = 1/180 (0%)
Frame = +2
Query: 47 MASTAAGSCNLSSSASISAFPFAGGARRSSSS-SSNSVVLSRKTRNLKVSAMAKELHFNK 223
MAST + +L S + +A + SSSS V R++R V A AKELHFNK
Sbjct: 1 MASTFTATSSLGSLLAPNAIKLSSATSISSSSFGRRHNVCVRRSRPAIVCA-AKELHFNK 59
Query: 224 DGSAIKRLQAGVNKLADLVGVTLGPKGRNVVLESKYGSPKIVNDGVTVAKEVELEDPVEN 403
DG+ I++LQ GVNKLADLVGVTLGPKGRNVVLESKYGSP+IVNDGVTVA+EVELEDPVEN
Sbjct: 60 DGTTIRKLQTGVNKLADLVGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREVELEDPVEN 119
Query: 404 IGAKLVRQAAAKTNDLAGDGTTTSVVLAQGLIAEGVKVVAAGANPVLITRGIEKTTKALV 583
IGAKLVRQAAAKTNDLAGDGTTTSVVLAQG IAEGVKVVAAGANPVLITRGIEKT KALV
Sbjct: 120 IGAKLVRQAAAKTNDLAGDGTTTSVVLAQGFIAEGVKVVAAGANPVLITRGIEKTAKALV 179
>dbj|BAA92724.1| ESTs C99033(E4350),C99032(E4350),D46006(S10372),
D47177(S12347),C28582(C61678),C27203(C51329) correspond
to a region of the predicted gene.~Similar to
Arabidopsis thaliana chromosome I BAC T5A14 genomic
sequence; 60 KD Chaperonin Beta subunit (AC005223)
[Oryza sativa (japonica cultivar-group)]
Length = 601
Score = 239 bits (611), Expect = 1e-62
Identities = 133/183 (72%), Positives = 148/183 (80%), Gaps = 9/183 (4%)
Frame = +2
Query: 62 AGSCNLSSSASISAFP--FAGGARRSSSSSSNSVVLSRKTRNLKVSAM-------AKELH 214
A + +S+ + A P + SS SS +SV ++ + RN ++ AKEL+
Sbjct: 2 ASTFGATSTVGLMAAPTGIVSDKKPSSLSSVSSVSVASRPRNARLQRKCNFRVKAAKELY 61
Query: 215 FNKDGSAIKRLQAGVNKLADLVGVTLGPKGRNVVLESKYGSPKIVNDGVTVAKEVELEDP 394
FNKDGSAIK+LQ GVNKLADLVGVTLGPKGRNVVLESKYGSP+IVNDGVTVA+EVELEDP
Sbjct: 62 FNKDGSAIKKLQTGVNKLADLVGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREVELEDP 121
Query: 395 VENIGAKLVRQAAAKTNDLAGDGTTTSVVLAQGLIAEGVKVVAAGANPVLITRGIEKTTK 574
VENIGAKLVRQAAAKTNDLAGDGTTTSVVLAQGLIAEGVKVVAAGANPV ITRGIEKT K
Sbjct: 122 VENIGAKLVRQAAAKTNDLAGDGTTTSVVLAQGLIAEGVKVVAAGANPVQITRGIEKTAK 181
Query: 575 ALV 583
ALV
Sbjct: 182 ALV 184
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 560,795,251
Number of Sequences: 1393205
Number of extensions: 15560791
Number of successful extensions: 438955
Number of sequences better than 10.0: 7803
Number of HSP's better than 10.0 without gapping: 118319
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 284046
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 21712003912
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
