>KMC009409A_C01 KMC009409A_c01
atacttggaattaaaGCCCCAAATGGTTGCCAAACGGCACTTACAACATGTGAGACTAAA
TCTCAGGGAGTGCTACAACAAATGCACTAAAACATAAGAAAATGAAACTCAAACATGCAC
ACTCAGCTGACTCAATCAGCATAAATCATGAAGTAAAACCTTAAAAGGCCAGACAGAAAC
CTCTCAAAACCCAAATTAACAGAAAGCTAAATAACTTCTAAGCAGTTAAAATATATCTTG
TTTGATCGGTGCTCCCAAAGCAACAACACAACCGGCTCGTGCCGGTGTTGCAAGTAGTGC
AGGCTTCAGCGGCAACAAAGGGGGGTCATCCAACAGATCTGACAACAAAGAGAAGGGAGA
AGCTCCCGCAGATCCCAAGCAAAACAGCAACAAAGGTTGCAAGGACGGGGGAAGCGGCAA
AATAAGGAGGCGACCGAGGTGGGGGCAGTGGCGGAAATGGGTGGCGCAGCGGTTGGACCA
GTAGCAGCAGCGGCGGCGGTGTTTACAGGTGATCGGAAGCAGAGGAGGATGAACATGTTC
A

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC009409A_C01 KMC009409A_c01
         (541 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

emb|CAB75628.1| hypothetical protein L8325.01 [Leishmania major]       38  0.004
ref|NP_180614.1| putative glycine-rich protein; protein id: At2g...    32  0.006
sp|Q9R0I7|ZAP3_MOUSE Nuclear protein ZAP3 gi|6016842|dbj|BAA8518...    30  0.055
ref|XP_126978.3| ZAP3 protein [Mus musculus]                           30  0.055
emb|CAD21122.1| related to lariat-debranching enzyme [Neurospora...    38  0.090

>emb|CAB75628.1| hypothetical protein L8325.01 [Leishmania major]
          Length = 664

 Score = 37.7 bits (86), Expect(2) = 0.004
 Identities = 29/69 (42%), Positives = 36/69 (52%), Gaps = 11/69 (15%)
 Frame = +2

Query: 263 TTQPARAGVASS----AGFSGNKGGSSNR----SDNKEKGEAPADPKQNSN---KGCKDG 409
           T +PA  G A+S    AG SG  G S+      SDN+E G     P+Q S+   +G K G
Sbjct: 359 TVRPAAKGPAASSSPAAGASGVLGTSAQGRGYGSDNEEAGNNAYMPQQPSSAVPRGAKAG 418

Query: 410 GSGKIRRRP 436
           GSG   RRP
Sbjct: 419 GSGAASRRP 427

 Score = 23.5 bits (49), Expect(2) = 0.004
 Identities = 12/29 (41%), Positives = 14/29 (47%), Gaps = 4/29 (13%)
 Frame = +3

Query: 426 GGDRGGGSGGNGWRS----GWTSSSSGGG 500
           GG  GGG G N W +    G    +S GG
Sbjct: 451 GGGGGGGGGMNDWANAYPPGMVPPTSTGG 479

>ref|NP_180614.1| putative glycine-rich protein; protein id: At2g30560.1 [Arabidopsis
           thaliana] gi|25296180|pir||H84709 probable glycine-rich
           protein [imported] - Arabidopsis thaliana
           gi|1946363|gb|AAB63081.1| putative glycine-rich protein
           [Arabidopsis thaliana]
          Length = 171

 Score = 32.0 bits (71), Expect(2) = 0.006
 Identities = 17/33 (51%), Positives = 18/33 (54%)
 Frame = +3

Query: 408 GEAAK*GGDRGGGSGGNGWRSGWTSSSSGGGVY 506
           G   K GG  GGG GG G +SG     SGGG Y
Sbjct: 110 GGGGKNGGGCGGGGGGKGGKSG---GGSGGGGY 139

 Score = 28.9 bits (63), Expect(2) = 0.006
 Identities = 18/50 (36%), Positives = 21/50 (42%), Gaps = 5/50 (10%)
 Frame = +2

Query: 284 GVASSAGFSGNKGG-----SSNRSDNKEKGEAPADPKQNSNKGCKDGGSG 418
           G    +G  G  GG      SNRS    +    +DPK  S  G K GG G
Sbjct: 38  GGGGKSGGGGGGGGYMVAPGSNRSSYISRDNFESDPKGGSGGGGKGGGGG 87

 Score = 28.1 bits (61), Expect(2) = 1.7
 Identities = 16/33 (48%), Positives = 18/33 (54%), Gaps = 1/33 (3%)
 Frame = +3

Query: 405 TGEAAK*GGDRGGGSGGN-GWRSGWTSSSSGGG 500
           +G   K GG  GG SGG  G +SG     SGGG
Sbjct: 77  SGGGGKGGGGGGGISGGGAGGKSGCGGGKSGGG 109

 Score = 24.3 bits (51), Expect(2) = 1.7
 Identities = 14/45 (31%), Positives = 14/45 (31%)
 Frame = +2

Query: 284 GVASSAGFSGNKGGSSNRSDNKEKGEAPADPKQNSNKGCKDGGSG 418
           G   S    G KGG    S     G      K     G K GG G
Sbjct: 3   GKGGSGSGGGGKGGGGGGSGGGRGGGGGGGAKGGCGGGGKSGGGG 47

>sp|Q9R0I7|ZAP3_MOUSE Nuclear protein ZAP3 gi|6016842|dbj|BAA85182.1| nuclear protein ZAP
           [Mus musculus]
          Length = 1386

 Score = 29.6 bits (65), Expect(2) = 0.055
 Identities = 19/43 (44%), Positives = 21/43 (48%)
 Frame = -2

Query: 411 PPSLQPLLLFCLGSAGASPFSLLSDLLDDPPLLPLKPALLATP 283
           PP L P     L SAG+ P      L   PP+LPL P   ATP
Sbjct: 551 PPVLPPP---ALSSAGSPPVLPPPALPGGPPILPLPPLSSATP 590

 Score = 27.7 bits (60), Expect(2) = 0.055
 Identities = 12/30 (40%), Positives = 15/30 (50%)
 Frame = -1

Query: 499 PPPLLLLVQPLRHPFPPLPPPRSPPYFAAS 410
           PPP++    P   P P +PPP  PP    S
Sbjct: 507 PPPVMPPALPTSIPPPGMPPPVMPPSLPTS 536

>ref|XP_126978.3| ZAP3 protein [Mus musculus]
          Length = 1386

 Score = 29.6 bits (65), Expect(2) = 0.055
 Identities = 19/43 (44%), Positives = 21/43 (48%)
 Frame = -2

Query: 411 PPSLQPLLLFCLGSAGASPFSLLSDLLDDPPLLPLKPALLATP 283
           PP L P     L SAG+ P      L   PP+LPL P   ATP
Sbjct: 551 PPVLPPP---ALSSAGSPPVLPPPALPGGPPILPLPPLSSATP 590

 Score = 27.7 bits (60), Expect(2) = 0.055
 Identities = 12/30 (40%), Positives = 15/30 (50%)
 Frame = -1

Query: 499 PPPLLLLVQPLRHPFPPLPPPRSPPYFAAS 410
           PPP++    P   P P +PPP  PP    S
Sbjct: 507 PPPVMPPALPTSIPPPGMPPPVMPPSLPTS 536

>emb|CAD21122.1| related to lariat-debranching enzyme [Neurospora crassa]
           gi|28917935|gb|EAA27620.1| hypothetical protein
           [Neurospora crassa]
          Length = 617

 Score = 37.7 bits (86), Expect = 0.090
 Identities = 19/51 (37%), Positives = 23/51 (44%)
 Frame = +3

Query: 348 KRREKLPQIPSKTATKVARTGEAAK*GGDRGGGSGGNGWRSGWTSSSSGGG 500
           +R  +  Q P   A +    G   + GG  GGG GG GWR G      GGG
Sbjct: 551 EREGRRVQGPRPMAERFGGRGGGHRGGGRGGGGGGGGGWRGGRGGRGGGGG 601

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 505,101,617
Number of Sequences: 1393205
Number of extensions: 12291288
Number of successful extensions: 125586
Number of sequences better than 10.0: 420
Number of HSP's better than 10.0 without gapping: 64852
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 108820
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 18462123008
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MFB084e04_f BP040151 1 541 2 MFB057b08_f BP038110 16 445

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