Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC009131A_C01 KMC009131A_c01
(548 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAC58012.1| S-adenosyl-L-methionine: daidzein 7-0-methyltran... 270 6e-72
pir||T09254 isoflavone-7-O-methyltransferase (EC 2.1.1.-) 9 - al... 259 1e-68
pir||T09707 isoflavone-O-methytransferase (EC 2.1.1.-) - alfalfa... 259 1e-68
gb|AAC49926.1| 7-O-methyltransferase [Medicago sativa] 259 1e-68
pir||T09299 o-methyltransferase (EC 2.1.1.-) iomt2003 - alfalfa ... 259 1e-68
>dbj|BAC58012.1| S-adenosyl-L-methionine: daidzein 7-0-methyltransferase
[Glycyrrhiza echinata]
Length = 357
Score = 270 bits (691), Expect = 6e-72
Identities = 129/175 (73%), Positives = 150/175 (85%)
Frame = +3
Query: 24 SSSKGRAASEIFKGQALVYRHLFAFENSMSLKWIVELGIPDIIHNHGHPITLSNLVSILQ 203
SS GR SEIF+GQAL+YRH++AF +SM LKWIVEL IP+IIHNHG PIT+S LVSIL+
Sbjct: 3 SSINGRKPSEIFQGQALLYRHIYAFIDSMCLKWIVELDIPNIIHNHGKPITVSELVSILK 62
Query: 204 IPQEKIGNVRRLMRYLAHNGFFEIVRIHEDQEEKEAYALTPASELLVKSSDMCLAPMVEC 383
+PQ K GNV+R+MRY+AHNGFFE VRI E+QEE EAYALT ASELLVK S++CLAPMVEC
Sbjct: 63 VPQTKAGNVQRIMRYMAHNGFFERVRIQEEQEENEAYALTAASELLVKGSELCLAPMVEC 122
Query: 384 ILDPTLSGSYHLLKEWIYEKDKTLFNVSLGSDIWDFLQKNPSYNSIFNEAMASDS 548
+LDPTLSGSYH LK+WIYE+D TLF VSLGS W+FL +NP YN FN+AMASDS
Sbjct: 123 VLDPTLSGSYHQLKKWIYEEDLTLFGVSLGSHFWEFLNENPEYNKSFNDAMASDS 177
>pir||T09254 isoflavone-7-O-methyltransferase (EC 2.1.1.-) 9 - alfalfa
gi|2580584|gb|AAC49927.1| 7-O-methyltransferase
[Medicago sativa]
Length = 352
Score = 259 bits (663), Expect = 1e-68
Identities = 126/175 (72%), Positives = 147/175 (84%)
Frame = +3
Query: 24 SSSKGRAASEIFKGQALVYRHLFAFENSMSLKWIVELGIPDIIHNHGHPITLSNLVSILQ 203
SS GR SEIFK QAL+Y+H++AF +SMSLKW V + IP+IIHNHG PI+LSNLVSILQ
Sbjct: 3 SSINGRKPSEIFKAQALLYKHIYAFIDSMSLKWAVGMNIPNIIHNHGKPISLSNLVSILQ 62
Query: 204 IPQEKIGNVRRLMRYLAHNGFFEIVRIHEDQEEKEAYALTPASELLVKSSDMCLAPMVEC 383
+P KIGNVRRLMRYLAHNGFFEI+ +E+E+YALT ASELLV+ SD+CLAPMVEC
Sbjct: 63 VPSSKIGNVRRLMRYLAHNGFFEIIT-----KEEESYALTVASELLVRGSDLCLAPMVEC 117
Query: 384 ILDPTLSGSYHLLKEWIYEKDKTLFNVSLGSDIWDFLQKNPSYNSIFNEAMASDS 548
+LDPTLSGSYH LK+WIYE+D TLF V+LGS WDFL KNP YN+ FN+AMASDS
Sbjct: 118 VLDPTLSGSYHELKKWIYEEDLTLFGVTLGSGFWDFLDKNPEYNTSFNDAMASDS 172
>pir||T09707 isoflavone-O-methytransferase (EC 2.1.1.-) - alfalfa
gi|13399463|pdb|1FP2|A Chain A, Crystal Structure
Analysis Of Isoflavone O-Methyltransferase
gi|2580540|gb|AAC49928.1| isoflavone-O-methytransferase
[Medicago sativa]
Length = 352
Score = 259 bits (662), Expect = 1e-68
Identities = 126/175 (72%), Positives = 147/175 (84%)
Frame = +3
Query: 24 SSSKGRAASEIFKGQALVYRHLFAFENSMSLKWIVELGIPDIIHNHGHPITLSNLVSILQ 203
SS GR SEIFK QAL+Y+H++AF +SMSLKW VE+ IP+II NHG PI+LSNLVSILQ
Sbjct: 3 SSINGRKPSEIFKAQALLYKHIYAFIDSMSLKWAVEMNIPNIIQNHGKPISLSNLVSILQ 62
Query: 204 IPQEKIGNVRRLMRYLAHNGFFEIVRIHEDQEEKEAYALTPASELLVKSSDMCLAPMVEC 383
+P KIGNVRRLMRYLAHNGFFEI+ +E+E+YALT ASELLV+ SD+CLAPMVEC
Sbjct: 63 VPSSKIGNVRRLMRYLAHNGFFEIIT-----KEEESYALTVASELLVRGSDLCLAPMVEC 117
Query: 384 ILDPTLSGSYHLLKEWIYEKDKTLFNVSLGSDIWDFLQKNPSYNSIFNEAMASDS 548
+LDPTLSGSYH LK+WIYE+D TLF V+LGS WDFL KNP YN+ FN+AMASDS
Sbjct: 118 VLDPTLSGSYHELKKWIYEEDLTLFGVTLGSGFWDFLDKNPEYNTSFNDAMASDS 172
>gb|AAC49926.1| 7-O-methyltransferase [Medicago sativa]
Length = 352
Score = 259 bits (662), Expect = 1e-68
Identities = 126/175 (72%), Positives = 147/175 (84%)
Frame = +3
Query: 24 SSSKGRAASEIFKGQALVYRHLFAFENSMSLKWIVELGIPDIIHNHGHPITLSNLVSILQ 203
SS GR SEIFK QAL+Y+H++AF +SMSLKW VE+ IP+II NHG PI+LSNLVSILQ
Sbjct: 3 SSINGRKPSEIFKAQALLYKHIYAFIDSMSLKWAVEMNIPNIIQNHGKPISLSNLVSILQ 62
Query: 204 IPQEKIGNVRRLMRYLAHNGFFEIVRIHEDQEEKEAYALTPASELLVKSSDMCLAPMVEC 383
+P KIGNVRRLMRYLAHNGFFEI+ +E+E+YALT ASELLV+ SD+CLAPMVEC
Sbjct: 63 VPSSKIGNVRRLMRYLAHNGFFEIIT-----KEEESYALTVASELLVRGSDLCLAPMVEC 117
Query: 384 ILDPTLSGSYHLLKEWIYEKDKTLFNVSLGSDIWDFLQKNPSYNSIFNEAMASDS 548
+LDPTLSGSYH LK+WIYE+D TLF V+LGS WDFL KNP YN+ FN+AMASDS
Sbjct: 118 VLDPTLSGSYHELKKWIYEEDLTLFGVTLGSGFWDFLDKNPEYNTSFNDAMASDS 172
>pir||T09299 o-methyltransferase (EC 2.1.1.-) iomt2003 - alfalfa
gi|2565273|gb|AAB88294.1| o-methytransferase [Medicago
sativa]
Length = 343
Score = 259 bits (662), Expect = 1e-68
Identities = 126/175 (72%), Positives = 147/175 (84%)
Frame = +3
Query: 24 SSSKGRAASEIFKGQALVYRHLFAFENSMSLKWIVELGIPDIIHNHGHPITLSNLVSILQ 203
SS GR SEIFK QAL+Y+H++AF +SMSLKW VE+ IP+II NHG PI+LSNLVSILQ
Sbjct: 3 SSINGRKPSEIFKAQALLYKHIYAFIDSMSLKWAVEMNIPNIIQNHGKPISLSNLVSILQ 62
Query: 204 IPQEKIGNVRRLMRYLAHNGFFEIVRIHEDQEEKEAYALTPASELLVKSSDMCLAPMVEC 383
+P KIGNVRRLMRYLAHNGFFEI+ +E+E+YALT ASELLV+ SD+CLAPMVEC
Sbjct: 63 VPSSKIGNVRRLMRYLAHNGFFEIIT-----KEEESYALTVASELLVRGSDLCLAPMVEC 117
Query: 384 ILDPTLSGSYHLLKEWIYEKDKTLFNVSLGSDIWDFLQKNPSYNSIFNEAMASDS 548
+LDPTLSGSYH LK+WIYE+D TLF V+LGS WDFL KNP YN+ FN+AMASDS
Sbjct: 118 VLDPTLSGSYHELKKWIYEEDLTLFGVTLGSGFWDFLDKNPEYNTSFNDAMASDS 172
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 498,495,968
Number of Sequences: 1393205
Number of extensions: 11108612
Number of successful extensions: 33425
Number of sequences better than 10.0: 70
Number of HSP's better than 10.0 without gapping: 32616
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 33363
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 18947112822
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
