KMC008611A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC008611A_C01 KMC008611A_c01
gacctgAGAAAAAAAATTATAATACATTAATTAAAATGGTATGAAATTTACAGCAAGTTT
ATAATACAGTGCTCAAAATGATCCATTCCAATCTCATAAATGTCAGATTCAACAAAGCTT
ACATATAGTTTAACTGGATATAATGTCTCAAGCTCACACAGAAACTATGTTGAGGAAGGA
GATCGATAAATCACTTCTCTATAAACGGTGCTAACGCACCACTTAAAATATCTTAGAACA
GAAAGGTAACAAAAGTTGCCTTCAGGCTCCTCTTCTGGTCTGACATGCACTCTTCATCGT
CCAAACATCGACCAGACAATGACTACATACATACATACATACATCCTAAAATTACCAAGA
TTGTACTGACTACTGCCAGTTCAGCCTACAAGCAAAGCTATTTCTATTTCATCTGTCAAA
CTATGAGAGCCTATCACCAGCACGTTTTGACAGCAATGCTGCCTTAACTATATTTCCCCT
TGTAATAAGTCCAACCAGCTTTCCATCTCTATCAACTACAGGCA


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC008611A_C01 KMC008611A_c01
         (524 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_195409.1| putative protein; protein id: At4g36910.1 [Arab...    52  3e-06
ref|NP_567952.1| putative protein; protein id: At4g34120.1, supp...    44  9e-04
pir||S25462 Ig kappa chain V region - mouse gi|938263|emb|CAA478...    35  0.54
ref|ZP_00072250.1| hypothetical protein [Trichodesmium erythraeu...    34  0.91
gb|AAB48727.1| anti-DNA immunoglobulin light chain IgG [Mus musc...    34  0.91

>ref|NP_195409.1| putative protein; protein id: At4g36910.1 [Arabidopsis thaliana]
           gi|25407775|pir||H85435 hypothetical protein AT4g36910
           [imported] - Arabidopsis thaliana
           gi|4006881|emb|CAB16799.1| putative protein [Arabidopsis
           thaliana] gi|7270640|emb|CAB80357.1| putative protein
           [Arabidopsis thaliana] gi|21537376|gb|AAM61717.1|
           unknown [Arabidopsis thaliana]
           gi|28392900|gb|AAO41886.1| unknown protein [Arabidopsis
           thaliana] gi|28827758|gb|AAO50723.1| unknown protein
           [Arabidopsis thaliana]
          Length = 236

 Score = 52.4 bits (124), Expect = 3e-06
 Identities = 24/31 (77%), Positives = 28/31 (89%)
 Frame = -3

Query: 522 PVVDRDGKLVGLITRGNIVKAALLSKRAGDR 430
           PVVD DGKLVG+ITRGN+V+AAL  KR+GDR
Sbjct: 204 PVVDSDGKLVGIITRGNVVRAALQIKRSGDR 234

>ref|NP_567952.1| putative protein; protein id: At4g34120.1, supported by cDNA:
           gi_13430837, supported by cDNA: gi_15810600 [Arabidopsis
           thaliana] gi|13430838|gb|AAK26041.1|AF360331_1 unknown
           protein [Arabidopsis thaliana]
           gi|15810601|gb|AAL07188.1| unknown protein [Arabidopsis
           thaliana]
          Length = 238

 Score = 44.3 bits (103), Expect = 9e-04
 Identities = 19/27 (70%), Positives = 24/27 (88%)
 Frame = -3

Query: 522 PVVDRDGKLVGLITRGNIVKAALLSKR 442
           PVVD DGKL+G++TRGN+V+AAL  KR
Sbjct: 206 PVVDADGKLIGILTRGNVVRAALQIKR 232

>pir||S25462 Ig kappa chain V region - mouse gi|938263|emb|CAA47881.1| IgG light
           chain V region [Mus musculus]
          Length = 91

 Score = 35.0 bits (79), Expect = 0.54
 Identities = 17/41 (41%), Positives = 22/41 (53%)
 Frame = +2

Query: 395 KLFLFHLSNYESLSPARFDSNAALTIFPLVISPTSFPSLST 517
           KL ++H SN ES  PARF  + + T F L I P      +T
Sbjct: 49  KLLIYHASNLESGVPARFSGSGSRTDFTLTIDPVEADDAAT 89

>ref|ZP_00072250.1| hypothetical protein [Trichodesmium erythraeum IMS101]
          Length = 153

 Score = 34.3 bits (77), Expect = 0.91
 Identities = 12/21 (57%), Positives = 20/21 (95%)
 Frame = -3

Query: 522 PVVDRDGKLVGLITRGNIVKA 460
           PVVD +GK++G++TRG+I++A
Sbjct: 129 PVVDENGKVIGILTRGDIIRA 149

>gb|AAB48727.1| anti-DNA immunoglobulin light chain IgG [Mus musculus]
          Length = 102

 Score = 34.3 bits (77), Expect = 0.91
 Identities = 16/41 (39%), Positives = 23/41 (56%)
 Frame = +2

Query: 395 KLFLFHLSNYESLSPARFDSNAALTIFPLVISPTSFPSLST 517
           KL ++  SN ES  PARF  + + T F L I+P     ++T
Sbjct: 46  KLLIYRASNLESXIPARFSGSGSRTDFTLTINPVEADDVAT 86

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 407,760,140
Number of Sequences: 1393205
Number of extensions: 7986985
Number of successful extensions: 16800
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 16245
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 16789
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 17019769648
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 MF059h10_f BP031437 1 470
2 MFB076c09_f BP039541 7 413
3 SPD056f05_f BP048473 22 399
4 SPD054g02_f BP048332 23 291
5 GNf034f09 BP069859 33 525




Lotus japonicus
Kazusa DNA Research Institute