>KMC008452A_C01 KMC008452A_c01
caaagattataatacattctaattaaatgattaatgttaattcataaaatatccaacatc
cctcactttactccgtgccaCATTCTCTTGACCACCCAAATTGTTATGTGATTTTGGGAC
AATTTGGAAGTTGTTGTATTTTTCTTTTCAAAGAGAATTATTATTGAAATAAAGAGAACA
TGAGAACACCCTTCTCATGAGATAGGTCATAGTTTATAGTCCACAAGACTTAGGAAAGGA
TAAAAGAAACTGAAATAAGAAAGAAAACAAGAAAAGAACCAAGTTCCAACGACAACTACA
AACAAAACACAATACAACATAAAACATCAGCAAAAAGAGGCTAACGAGATTGAACAGCAT
CAGGACAGCCAAAAAGATGGGACCAGTGGCAGCAAGCATCTAATCCTGCGCAGTAGACAT
ATCCAAATTAAAGGCTTTGTCCCAAGACTTTGGGACTTCTATTCATCAGACCTCCATGGA
CATTCTGGCACTTCTTTCCAGTCGGATGAGATTGTCAATCTATTATAATCTCCTTGCAAC
GCATCCTGCACAGTAGACATATCCAAAGGCTTTGTCCCAAGACTTTGGACTTCTGGGTGA
ACTAACCCTGATGTTCTCTCAACCTTGACAGCATCCCTACTTTCCTCCTTA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC008452A_C01 KMC008452A_c01
         (651 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

pir||E22845 hypothetical protein 4 - Trypanosoma brucei mitochon...    37  0.18
ref|NP_702802.1| hypothetical protein [Plasmodium falciparum 3D7...    37  0.30
gb|AAN86606.1| MURF1 [Trypanosoma sp.]                                 36  0.51
gb|EAA22516.1| hypothetical protein [Plasmodium yoelii yoelii]         35  1.1
ref|NP_050068.1| NH2 terminus uncertain [Leishmania tarentolae] ...    35  1.1

>pir||E22845 hypothetical protein 4 - Trypanosoma brucei mitochondrion
          Length = 445

 Score = 37.4 bits (85), Expect = 0.18
 Identities = 28/103 (27%), Positives = 52/103 (50%), Gaps = 8/103 (7%)
 Frame = -2

Query: 431 LIWICLLRRIRCLLPLVPSFWLS*-CCSISLASFC*CFMLYCVLFVVVVGTWFFSCFLSY 255
           L+  CL   I  + P+V  F++   C  + L+  C C   + +++++++  ++F   ++ 
Sbjct: 341 LLLACLFLCIGAI-PIVFGFFIKVFCLLLQLSYLCICIGFFFIIWLIIIYIFYFRLIVNI 399

Query: 254 FSFFYPFLSLVDYKL*PIS*EG-----CS--HVLFISIIILFE 147
           F F Y FL     KL  I+ +      CS  ++LF  II LF+
Sbjct: 400 FIFSYQFLGFWVVKLSFINIKNLLFFICSSVYILFFDIINLFD 442

>ref|NP_702802.1| hypothetical protein [Plasmodium falciparum 3D7]
           gi|23498218|emb|CAD49189.1| hypothetical protein
           [Plasmodium falciparum 3D7]
          Length = 104

 Score = 36.6 bits (83), Expect = 0.30
 Identities = 28/71 (39%), Positives = 37/71 (51%)
 Frame = -1

Query: 375 FLAVLMLFNLVSLFLLMFYVVLCFVCSCRWNLVLFLFSFLFQFLLSFPKSCGL*TMTYLM 196
           FL+ L+ F L  L   +   +L F+ S    L+ FL SFL  FLLSF   C L    YL+
Sbjct: 33  FLSFLLSFLLSFLLSFLLSFLLSFLLSF---LLSFLLSFLLSFLLSFLLICFL---NYLL 86

Query: 195 RRVFSCSLYFN 163
              FS SL+ +
Sbjct: 87  SFSFSFSLFLS 97

>gb|AAN86606.1| MURF1 [Trypanosoma sp.]
          Length = 97

 Score = 35.8 bits (81), Expect = 0.51
 Identities = 22/82 (26%), Positives = 43/82 (51%), Gaps = 1/82 (1%)
 Frame = -2

Query: 392 LPLVPSFWLS*CCSISLASFC*-CFMLYCVLFVVVVGTWFFSCFLSYFSFFYPFLSLVDY 216
           +P+V  F+L   C +   S+   C   + +L+++VV  ++F   ++ F F Y F+     
Sbjct: 5   IPIVFGFFLKVFCLLLQLSYLGVCVTFFLMLWLIVVYIFYFRLIVNIFIFSYQFIGFWVV 64

Query: 215 KL*PIS*EGCSHVLFISIIILF 150
           +L  ++ +    VL I++ ILF
Sbjct: 65  RLYVVTFQNLFFVLAINLYILF 86

>gb|EAA22516.1| hypothetical protein [Plasmodium yoelii yoelii]
          Length = 1668

 Score = 34.7 bits (78), Expect = 1.1
 Identities = 22/81 (27%), Positives = 39/81 (47%), Gaps = 1/81 (1%)
 Frame = +2

Query: 194 LMR*VIVYSPQDLGKDKRN*NKKENKKRTKFQRQLQTKHNTT*NISKKR-LTRLNSIRTA 370
           LM   ++    ++G  +R  N +      K+  +L  +H+TT  I KKR    +N  +  
Sbjct: 620 LMNKNVIIDTVNVGNKRRGNNLQTKYSDEKYNNELSEEHDTTHLIDKKRKFNDINEHKNN 679

Query: 371 KKMGPVAASI*SCAVDISKLK 433
            K G    +I +C V+ +K+K
Sbjct: 680 SKNGKYNGTIKACDVNENKIK 700

>ref|NP_050068.1| NH2 terminus uncertain [Leishmania tarentolae] gi|83996|pir||B26696
           hypothetical protein 1 (CYb-COII intergenic region) -
           Leishmania tarentolae mitochondrion (fragment)
           gi|896286|gb|AAA96601.1| NH2 terminus uncertain
          Length = 443

 Score = 34.7 bits (78), Expect = 1.1
 Identities = 19/82 (23%), Positives = 41/82 (49%), Gaps = 1/82 (1%)
 Frame = -2

Query: 392 LPLVPSFWLS*CCSISLASFC*-CFMLYCVLFVVVVGTWFFSCFLSYFSFFYPFLSLVDY 216
           +P+V  F+L   C +   S+   C + + +++++++  ++F   ++ F F Y F+     
Sbjct: 351 IPIVFGFFLKVFCLLLHLSYLGICIVFFSIIWLIIIYIFYFRLIINIFIFSYQFIGFWVV 410

Query: 215 KL*PIS*EGCSHVLFISIIILF 150
           +L  +       +L  SI ILF
Sbjct: 411 RLHILGFSNLFFILSFSIFILF 432

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 557,439,332
Number of Sequences: 1393205
Number of extensions: 11982493
Number of successful extensions: 35881
Number of sequences better than 10.0: 63
Number of HSP's better than 10.0 without gapping: 32705
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 35716
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 27860523586
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MF074b09_f BP032213 1 549 2 GNf041g08 BP070418 144 574 3 MF057f07_f BP031310 144 482 4 MF075e10_f BP032281 149 665 5 GNf023c07 BP069026 152 534 6 SPD072e08_f BP049764 165 369

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