Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC008361A_C01 KMC008361A_c01
(410 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAO41898.1| putative biotin synthase (Bio B) [Arabidopsis tha... 156 5e-38
ref|NP_181864.1| biotin synthase (Bio B); protein id: At2g43360.... 156 5e-38
gb|EAA27328.1| hypothetical protein [Neurospora crassa] 96 8e-20
ref|NP_587728.1| biotin synthase (EC 2.8.1.6) [Schizosaccharomyc... 95 2e-19
emb|CAA12229.1| Biotin synthase [Schizosaccharomyces pombe] 95 2e-19
>gb|AAO41898.1| putative biotin synthase (Bio B) [Arabidopsis thaliana]
Length = 378
Score = 156 bits (395), Expect = 5e-38
Identities = 84/138 (60%), Positives = 102/138 (73%), Gaps = 4/138 (2%)
Frame = +2
Query: 8 MFLVRPIFRTHPKPSSLGVLH----YSSSSPAAIQAERTIKQGPRKDRTRDEVKSIYDSP 175
M LVR +FR+ +PS G L YSS S A+ +AERTI++GPR D +RDE+KS+YDSP
Sbjct: 1 MMLVRSVFRSQLRPSVSGGLQSASCYSSLSAASAEAERTIREGPRNDWSRDEIKSVYDSP 60
Query: 176 VLVSSRVPVGLKFTDMLITSGEVQQCTLLSVKTGGCSEDCSYCPQSSRYDTGLKAQRLLE 355
+L + G + + EVQQCTLLS+KTGGCSEDCSYCPQSSRY TG+KAQRL+
Sbjct: 61 LL--DLLFHGAQVHRHVHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYSTGVKAQRLMS 118
Query: 356 KEAVIQAANKAKEAGSTR 409
K+AVI AA KAKEAGSTR
Sbjct: 119 KDAVIDAAKKAKEAGSTR 136
>ref|NP_181864.1| biotin synthase (Bio B); protein id: At2g43360.1, supported by
cDNA: 42038., supported by cDNA: gi_1403661, supported
by cDNA: gi_1769456 [Arabidopsis thaliana]
gi|1705463|sp|P54967|BIOB_ARATH Biotin synthase (Biotin
synthetase) gi|2129547|pir||S71201 biotin synthase (EC
2.8.1.6) bioB [similarity] - Arabidopsis thaliana
gi|1045316|gb|AAA80226.1| biotin sythase
gi|1403662|gb|AAC49445.1| BIO2 protein
gi|1769457|gb|AAB39953.1| biotin synthase [Arabidopsis
thaliana] gi|2288983|gb|AAB64312.1| biotin synthase (Bio
B) [Arabidopsis thaliana] gi|1589016|prf||2209438A
biotin synthase
Length = 378
Score = 156 bits (395), Expect = 5e-38
Identities = 84/138 (60%), Positives = 102/138 (73%), Gaps = 4/138 (2%)
Frame = +2
Query: 8 MFLVRPIFRTHPKPSSLGVLH----YSSSSPAAIQAERTIKQGPRKDRTRDEVKSIYDSP 175
M LVR +FR+ +PS G L YSS S A+ +AERTI++GPR D +RDE+KS+YDSP
Sbjct: 1 MMLVRSVFRSQLRPSVSGGLQSASCYSSLSAASAEAERTIREGPRNDWSRDEIKSVYDSP 60
Query: 176 VLVSSRVPVGLKFTDMLITSGEVQQCTLLSVKTGGCSEDCSYCPQSSRYDTGLKAQRLLE 355
+L + G + + EVQQCTLLS+KTGGCSEDCSYCPQSSRY TG+KAQRL+
Sbjct: 61 LL--DLLFHGAQVHRHVHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYSTGVKAQRLMS 118
Query: 356 KEAVIQAANKAKEAGSTR 409
K+AVI AA KAKEAGSTR
Sbjct: 119 KDAVIDAAKKAKEAGSTR 136
>gb|EAA27328.1| hypothetical protein [Neurospora crassa]
Length = 439
Score = 96.3 bits (238), Expect = 8e-20
Identities = 50/97 (51%), Positives = 66/97 (67%), Gaps = 2/97 (2%)
Frame = +2
Query: 125 PRKDRTRDEVKSIYDSPV--LVSSRVPVGLKFTDMLITSGEVQQCTLLSVKTGGCSEDCS 298
PR D T++E++ IY +P+ LV S + +F D VQ CTL+++KTGGCSEDCS
Sbjct: 81 PRNDWTKEEIREIYQTPLMELVYSASTLHRRFFD----PASVQLCTLMNIKTGGCSEDCS 136
Query: 299 YCPQSSRYDTGLKAQRLLEKEAVIQAANKAKEAGSTR 409
YC QSSRY TG+KA ++ + V+QAA AKE GSTR
Sbjct: 137 YCAQSSRYKTGVKATKISSVDEVLQAAKIAKENGSTR 173
>ref|NP_587728.1| biotin synthase (EC 2.8.1.6) [Schizosaccharomyces pombe]
gi|12643615|sp|O59778|BIOB_SCHPO Biotin synthase (Biotin
synthetase) gi|7490157|pir||T40876 biotin synthase (EC
2.8.1.6) - fission yeast (Schizosaccharomyces pombe)
gi|2995363|emb|CAA18303.1| biotin synthase (EC 2.8.1.6)
[Schizosaccharomyces pombe] gi|3668149|emb|CAA21106.1|
biotin synthase. [Schizosaccharomyces pombe]
Length = 363
Score = 94.7 bits (234), Expect = 2e-19
Identities = 48/96 (50%), Positives = 67/96 (69%), Gaps = 2/96 (2%)
Frame = +2
Query: 128 RKDRTRDEVKSIYDSPV--LVSSRVPVGLKFTDMLITSGEVQQCTLLSVKTGGCSEDCSY 301
R + TR+E++ IYD+P+ L+ + KF D +VQQCTLLS+KTGGC+EDC Y
Sbjct: 20 RNNWTREEIQKIYDTPLIDLIFRAASIHRKFHD----PKKVQQCTLLSIKTGGCTEDCKY 75
Query: 302 CPQSSRYDTGLKAQRLLEKEAVIQAANKAKEAGSTR 409
C QSSRY+TG+KA +L++ + V++ A AK GSTR
Sbjct: 76 CAQSSRYNTGVKATKLMKIDEVLEKAKIAKAKGSTR 111
>emb|CAA12229.1| Biotin synthase [Schizosaccharomyces pombe]
Length = 362
Score = 94.7 bits (234), Expect = 2e-19
Identities = 48/96 (50%), Positives = 67/96 (69%), Gaps = 2/96 (2%)
Frame = +2
Query: 128 RKDRTRDEVKSIYDSPV--LVSSRVPVGLKFTDMLITSGEVQQCTLLSVKTGGCSEDCSY 301
R + TR+E++ IYD+P+ L+ + KF D +VQQCTLLS+KTGGC+EDC Y
Sbjct: 20 RNNWTREEIQKIYDTPLIDLIFRAASIHRKFHD----PKKVQQCTLLSIKTGGCTEDCKY 75
Query: 302 CPQSSRYDTGLKAQRLLEKEAVIQAANKAKEAGSTR 409
C QSSRY+TG+KA +L++ + V++ A AK GSTR
Sbjct: 76 CAQSSRYNTGVKATKLMKIDEVLEKAKIAKAKGSTR 111
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 389,192,076
Number of Sequences: 1393205
Number of extensions: 8387641
Number of successful extensions: 23198
Number of sequences better than 10.0: 143
Number of HSP's better than 10.0 without gapping: 22493
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 23181
length of database: 448,689,247
effective HSP length: 112
effective length of database: 292,650,287
effective search space used: 7023606888
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
