Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC008345A_C01 KMC008345A_c01
(486 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_190233.1| putative protein; protein id: At3g46490.1 [Arab... 75 4e-31
ref|NP_174753.1| hyoscyamine 6-dioxygenase hydroxylase, putative... 83 2e-26
gb|AAK49616.1|AF372900_1 At1g35190/T32G9_27 [Arabidopsis thaliana] 83 2e-26
ref|NP_190232.1| putative protein; protein id: At3g46480.1 [Arab... 71 6e-24
ref|NP_190234.1| putative protein; protein id: At3g46500.1 [Arab... 63 9e-18
>ref|NP_190233.1| putative protein; protein id: At3g46490.1 [Arabidopsis thaliana]
gi|11255893|pir||T45586 hypothetical protein F12A12.10 -
Arabidopsis thaliana gi|6523052|emb|CAB62319.1| putative
protein [Arabidopsis thaliana]
Length = 306
Score = 74.7 bits (182), Expect(3) = 4e-31
Identities = 33/51 (64%), Positives = 39/51 (75%)
Frame = +1
Query: 238 DEVFAQSKKLFTLPHDEKMKLLRNDKHRGYTPKHDEILDPENQVHGDIQGG 390
DE F SKK F LP +EKMK+LRN+K+RGY P HD +LDPENQV GD + G
Sbjct: 57 DEAFEHSKKFFALPLEEKMKVLRNEKYRGYAPFHDSLLDPENQVRGDYKEG 107
Score = 60.8 bits (146), Expect(3) = 4e-31
Identities = 29/59 (49%), Positives = 39/59 (65%)
Frame = +3
Query: 72 QNPEHEEEAPKPQAVLNCIDLSNPNINQSVDLLKQASTDSGFFYLVNHGISQEVHGRGF 248
+N EE + + L CIDL N +++QS LLKQA DSGFFY++NHGIS+E+ F
Sbjct: 2 ENKTEEEVSIIKVSSLTCIDLDNSDLHQSAVLLKQACLDSGFFYVINHGISEELKDEAF 60
Score = 40.8 bits (94), Expect(3) = 4e-31
Identities = 15/35 (42%), Positives = 24/35 (67%)
Frame = +2
Query: 380 YKEGYDMGVEVAENDPQSQKPWYGPCMWPASDILP 484
YKEG+ +G E +++ P KP++ P +WP D+LP
Sbjct: 104 YKEGFTIGFEGSKDGPHWDKPFHSPNIWPNPDVLP 138
>ref|NP_174753.1| hyoscyamine 6-dioxygenase hydroxylase, putative; protein id:
At1g35190.1, supported by cDNA: 2551., supported by
cDNA: gi_13926296 [Arabidopsis thaliana]
gi|25285682|pir||G86472 probable hyoscyamine
6-dioxygenase hydroxylase [imported] - Arabidopsis
thaliana gi|12320957|gb|AAG50602.1|AC079605_7
hyoscyamine 6-dioxygenase hydroxylase, putative
[Arabidopsis thaliana]
Length = 329
Score = 83.2 bits (204), Expect(2) = 2e-26
Identities = 37/56 (66%), Positives = 47/56 (83%)
Frame = +1
Query: 223 ARRFMDEVFAQSKKLFTLPHDEKMKLLRNDKHRGYTPKHDEILDPENQVHGDIQGG 390
+ FMD+VF QSKKLF LP +EKMK+LRN+KHRGYTP DE+LDP+NQ++GD + G
Sbjct: 47 SEEFMDDVFEQSKKLFALPLEEKMKVLRNEKHRGYTPVLDELLDPKNQINGDHKEG 102
Score = 57.0 bits (136), Expect(2) = 2e-26
Identities = 25/38 (65%), Positives = 31/38 (80%)
Frame = +3
Query: 117 LNCIDLSNPNINQSVDLLKQASTDSGFFYLVNHGISQE 230
LNCIDL+N ++N SV LKQA D GFFY++NHGIS+E
Sbjct: 12 LNCIDLANDDLNHSVVSLKQACLDCGFFYVINHGISEE 49
Score = 50.4 bits (119), Expect = 1e-05
Identities = 20/42 (47%), Positives = 29/42 (68%)
Frame = +2
Query: 359 KTKYTETYKEGYDMGVEVAENDPQSQKPWYGPCMWPASDILP 484
K + +KEGY +G+EV ++DP KP+YGP WP +D+LP
Sbjct: 92 KNQINGDHKEGYYIGIEVPKDDPHWDKPFYGPNPWPDADVLP 133
>gb|AAK49616.1|AF372900_1 At1g35190/T32G9_27 [Arabidopsis thaliana]
Length = 146
Score = 83.2 bits (204), Expect(2) = 2e-26
Identities = 37/56 (66%), Positives = 47/56 (83%)
Frame = +1
Query: 223 ARRFMDEVFAQSKKLFTLPHDEKMKLLRNDKHRGYTPKHDEILDPENQVHGDIQGG 390
+ FMD+VF QSKKLF LP +EKMK+LRN+KHRGYTP DE+LDP+NQ++GD + G
Sbjct: 47 SEEFMDDVFEQSKKLFALPLEEKMKVLRNEKHRGYTPVLDELLDPKNQINGDHKEG 102
Score = 57.0 bits (136), Expect(2) = 2e-26
Identities = 25/38 (65%), Positives = 31/38 (80%)
Frame = +3
Query: 117 LNCIDLSNPNINQSVDLLKQASTDSGFFYLVNHGISQE 230
LNCIDL+N ++N SV LKQA D GFFY++NHGIS+E
Sbjct: 12 LNCIDLANDDLNHSVVSLKQACLDCGFFYVINHGISEE 49
Score = 50.4 bits (119), Expect = 1e-05
Identities = 20/42 (47%), Positives = 29/42 (68%)
Frame = +2
Query: 359 KTKYTETYKEGYDMGVEVAENDPQSQKPWYGPCMWPASDILP 484
K + +KEGY +G+EV ++DP KP+YGP WP +D+LP
Sbjct: 92 KNQINGDHKEGYYIGIEVPKDDPHWDKPFYGPNPWPDADVLP 133
>ref|NP_190232.1| putative protein; protein id: At3g46480.1 [Arabidopsis thaliana]
gi|11255898|pir||T45705 hypothetical protein F18L15.200
- Arabidopsis thaliana gi|6522627|emb|CAB62039.1|
putative protein [Arabidopsis thaliana]
Length = 297
Score = 70.9 bits (172), Expect(2) = 6e-24
Identities = 32/44 (72%), Positives = 37/44 (83%)
Frame = +1
Query: 238 DEVFAQSKKLFTLPHDEKMKLLRNDKHRGYTPKHDEILDPENQV 369
DE F QSKK F LP +EKMK+LRN+KHRGY+P D+ILDPENQV
Sbjct: 57 DEAFEQSKKFFALPLEEKMKVLRNEKHRGYSPVLDQILDPENQV 100
Score = 60.8 bits (146), Expect(2) = 6e-24
Identities = 29/59 (49%), Positives = 36/59 (60%)
Frame = +3
Query: 72 QNPEHEEEAPKPQAVLNCIDLSNPNINQSVDLLKQASTDSGFFYLVNHGISQEVHGRGF 248
+N EE + + L CIDL+NPN QS LKQA D GFFY+ NHGIS+E+ F
Sbjct: 2 ENKTQEEASTIKVSSLTCIDLANPNFQQSAVSLKQACLDCGFFYVTNHGISEELKDEAF 60
>ref|NP_190234.1| putative protein; protein id: At3g46500.1 [Arabidopsis thaliana]
gi|11255904|pir||T45587 hypothetical protein F12A12.20 -
Arabidopsis thaliana gi|6523053|emb|CAB62320.1| putative
protein [Arabidopsis thaliana]
Length = 296
Score = 62.8 bits (151), Expect(2) = 9e-18
Identities = 29/47 (61%), Positives = 36/47 (75%)
Frame = +1
Query: 238 DEVFAQSKKLFTLPHDEKMKLLRNDKHRGYTPKHDEILDPENQVHGD 378
DE F QSKK F LP DEKMK+L+N+KH+GY+P +I D NQ+HGD
Sbjct: 68 DEAFEQSKKFFALPLDEKMKVLKNEKHQGYSPVLSQISD--NQIHGD 112
Score = 48.1 bits (113), Expect(2) = 9e-18
Identities = 27/70 (38%), Positives = 37/70 (52%), Gaps = 11/70 (15%)
Frame = +3
Query: 72 QNPEHEEEAPKPQAVLNCIDLSNPNINQSVDLLKQA-----------STDSGFFYLVNHG 218
+N EE + + L CIDL+N N+++S LKQA D GFFY+ NHG
Sbjct: 2 ENKTQEEASTINVSSLACIDLANSNLHRSAASLKQAWLFFFLFLNVNDYDCGFFYVTNHG 61
Query: 219 ISQEVHGRGF 248
IS+E+ F
Sbjct: 62 ISEELKDEAF 71
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 441,355,293
Number of Sequences: 1393205
Number of extensions: 9225661
Number of successful extensions: 31039
Number of sequences better than 10.0: 151
Number of HSP's better than 10.0 without gapping: 30042
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 31024
length of database: 448,689,247
effective HSP length: 113
effective length of database: 291,257,082
effective search space used: 13980339936
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
