>KMC008277A_C01 KMC008277A_c01
tcaaaactaggtattaaaatattagaatTTCTAAGTATGACAATATCTTTTCCTAGACAA
ATATAATATTCACTACCATGGCAACATAACTTAAAATATTCACCAAATTTAAGAGACTTC
ATTATGCTAATAAGGTACAAATCAAATATGGTCTGAGAATTTATGTGATCTTGGCAATTG
CCATCCAAGAGTGCATTTTCTCTCTCAATCTTGTCCATCTTCTTAGTCTTGACAAGGAGA
AACTAGACTTGGTACATAACCATAGGCATCCATCCAAAATACTTCGTTGGGATTTGGAAT
ATACTCCATACTATAATCTCTTATATTATACTTTATAAACTCCAAATTCTCTGTTATTGC
GATCATAAGAATGTCACCATCTTCTAACAAGAACATCGGCACTGGGATATACGGAAATCC
AATACAATGAAGACGCTCATAACTGATACTCAACAACTGAGTCCAAGACTCTAGAGCTCC
ATACTTTACCATTTTCCACACAAATAAATGGATACTGTTATGGTCATGGAAAAGGTACAT
GTGATTCCCTAGAACCTTAAGACTTGGCTCAAAACGAG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC008277A_C01 KMC008277A_c01
         (578 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_199849.1| putative protein; protein id: At5g50380.1 [Arab...    35  0.52
ref|NP_189039.1| hypothetical protein; protein id: At3g23970.1 [...    34  1.2
gb|EAA41029.1| GLP_12_49813_66465 [Giardia lamblia ATCC 50803]         33  2.6
gb|EAA21325.1| hypothetical protein [Plasmodium yoelii yoelii]         33  2.6

>ref|NP_199849.1| putative protein; protein id: At5g50380.1 [Arabidopsis thaliana]
           gi|9758920|dbj|BAB09457.1|
           gb|AAD25781.1~gene_id:MXI22.10~strong similarity to
           unknown protein [Arabidopsis thaliana]
          Length = 637

 Score = 35.4 bits (80), Expect = 0.52
 Identities = 17/49 (34%), Positives = 25/49 (50%), Gaps = 2/49 (4%)
 Frame = -2

Query: 304 VYSKSQRSILDGCLWLCTKSSFSLSRLRR--WTRLREKMHSWMAIAKIT 164
           VYS  +R  LD CL +      S+  +++  W  + EKM  W+   KIT
Sbjct: 219 VYSSVRRDALDDCLMILGVEKLSIEEVQKIDWKSMDEKMKKWIQAVKIT 267

>ref|NP_189039.1| hypothetical protein; protein id: At3g23970.1 [Arabidopsis
           thaliana] gi|9294666|dbj|BAB03015.1|
           gene_id:F14O13.16~pir||T05437~similar to unknown protein
           [Arabidopsis thaliana]
          Length = 413

 Score = 34.3 bits (77), Expect = 1.2
 Identities = 20/72 (27%), Positives = 36/72 (49%), Gaps = 1/72 (1%)
 Frame = -3

Query: 531 FHDHNSIHLFVWKMVKY-GALESWTQLLSISYERLHCIGFPYIPVPMFLLEDGDILMIAI 355
           F ++ +  L VW++VKY G  E+W     +S   +  +G  Y PV M  L+  +I+ +  
Sbjct: 280 FGENGNRRLRVWRLVKYTGGPEAWQLFWEVSLASVTKLGIDYFPVVMHPLK-SEIIYLWS 338

Query: 354 TENLEFIKYNIR 319
                 + +N+R
Sbjct: 339 RNKKGMVLFNLR 350

>gb|EAA41029.1| GLP_12_49813_66465 [Giardia lamblia ATCC 50803]
          Length = 5550

 Score = 33.1 bits (74), Expect = 2.6
 Identities = 21/82 (25%), Positives = 35/82 (42%), Gaps = 1/82 (1%)
 Frame = -3

Query: 537  YLFHDHNSIHLFVWKMVKYGALESWTQLLSISYERLHCIGFPYIPVPMFLLEDGDILMIA 358
            +LF  HN    +  K V  G  +                 F Y P P+ L+E+    ++ 
Sbjct: 5126 FLFDTHNPFSFYYKKAVAQGDTD-----------------FDYAPPPIVLIENSGNQVVV 5168

Query: 357  ITENL-EFIKYNIRDYSMEYIP 295
            +TE + + +KY+IR Y    +P
Sbjct: 5169 LTEMITKLVKYSIRKYGAAGLP 5190

>gb|EAA21325.1| hypothetical protein [Plasmodium yoelii yoelii]
          Length = 2112

 Score = 33.1 bits (74), Expect = 2.6
 Identities = 21/74 (28%), Positives = 33/74 (44%), Gaps = 8/74 (10%)
 Frame = -1

Query: 200  ENALLDGNCQDHINSQTIFDLYLISIMKSLK--------FGEYFKLCCHGSEYYICLGKD 45
            EN+L D N  + INS  + ++YL +  K           F    ++  + S+YY C    
Sbjct: 814  ENSLRDNNNYNSINSNIVTNMYLSNNSKKYNSVSNSCHLFNNKNEMSNNSSKYYACKNGP 873

Query: 44   IVILRNSNILIPSF 3
              +L  SN L P +
Sbjct: 874  YNVLNKSNTLHPYY 887

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 489,213,080
Number of Sequences: 1393205
Number of extensions: 10374938
Number of successful extensions: 25418
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 24652
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25412
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 21426319650
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 SPD051d12_f BP048073 1 344 2 GNf014h04 BP068414 29 504 3 MFB097f12_f BP041084 49 580 4 SPD089a07_f BP051078 52 551

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