>KMC007696A_C01 KMC007696A_c01
acgccactgctccaaacaacgagtgcggccATTCCCATCCATCTTCATCTTCCTCTTCCA
GGGAGTTTGATTCCTGTGTATTCTCAAAATGACTTCGTTTTTGAAACCTGAAAACGCCCT
GAAAAGGGCGGAAGAGCTGATCAATGTGGGGCAGAAGCAGGATGCTTTGCAGACCCTCCA
TGATGTTATTACTTCGAAGAGATATAGGGCTTGGCAGAAGACCCTTGAGAGGATTATGTT
CAAGTATGTTGAACTGTGCGTGGAGCTGCGCAAAGGGAGGTTTGCGAAGGATGGGCTTAT
CCAATATCGTATCATATGTCAGCAAGTGAATGTTAGCTCGCTGGAGGAGGTTATCAAACA
TTTCATGCACTTGTCGACTGAGAAAGCTGAACAAGCCCGTAGCCAGGCCCAGGCATTGGA
AGAAGCGCTTGATGTTGATGATCTGGAGGCTGATAAGAGGCCTGAGGACTTGATGCTAAG
CT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC007696A_C01 KMC007696A_c01
         (482 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

sp|Q40554|IF3A_TOBAC Eukaryotic translation initiation factor 3 ...   231  2e-60
ref|NP_192881.1| putative protein; protein id: At4g11420.1, supp...   229  2e-59
dbj|BAB07943.1| putative eukaryotic translation initiation facto...   228  3e-59
sp|Q9XHR2|IF3A_MAIZE Eukaryotic translation initiation factor 3 ...   224  5e-58
sp|P23116|IF3A_MOUSE Eukaryotic translation initiation factor 3 ...   140  7e-33

>sp|Q40554|IF3A_TOBAC Eukaryotic translation initiation factor 3 subunit 10 (eIF-3 theta)
           (Eukaryotic translation initiation factor 3 large
           subunit) (eIF3a) (PNLA-35) gi|629692|pir||S47179
           hypothetical protein - common tobacco
           gi|506471|emb|CAA56189.1| unnamed protein product
           [Nicotiana tabacum]
          Length = 958

 Score =  231 bits (590), Expect = 2e-60
 Identities = 112/131 (85%), Positives = 126/131 (95%)
 Frame = +2

Query: 89  MTSFLKPENALKRAEELINVGQKQDALQTLHDVITSKRYRAWQKTLERIMFKYVELCVEL 268
           M +F KPENALKRAEELI VGQKQ+ALQ LHD+ITS+RYRAWQKTLERIMFKYVELCV++
Sbjct: 1   MATFAKPENALKRAEELITVGQKQEALQALHDLITSRRYRAWQKTLERIMFKYVELCVDM 60

Query: 269 RKGRFAKDGLIQYRIICQQVNVSSLEEVIKHFMHLSTEKAEQARSQAQALEEALDVDDLE 448
           R+GRFAKDGLIQYRI+CQQVN++SLEEVIKHFMHL+TE+AE AR+QAQALEEALDV+DLE
Sbjct: 61  RRGRFAKDGLIQYRIVCQQVNINSLEEVIKHFMHLATERAELARNQAQALEEALDVEDLE 120

Query: 449 ADKRPEDLMLS 481
           ADKRPEDLMLS
Sbjct: 121 ADKRPEDLMLS 131

>ref|NP_192881.1| putative protein; protein id: At4g11420.1, supported by cDNA:
           gi_12407748 [Arabidopsis thaliana]
           gi|23396624|sp|Q9LD55|IF3A_ARATH Eukaryotic translation
           initiation factor 3 subunit 10 (eIF-3 theta) (Eukaryotic
           translation initiation factor 3 large subunit) (eIF3a)
           (p114) gi|7486118|pir||T10562 hypothetical protein
           F25E4.40 - Arabidopsis thaliana
           gi|7267841|emb|CAB81243.1| putative protein [Arabidopsis
           thaliana] gi|7321039|emb|CAB82147.1| putative protein
           [Arabidopsis thaliana]
           gi|12407749|gb|AAG53635.1|AF291711_1 initiation factor
           3a [Arabidopsis thaliana]
          Length = 987

 Score =  229 bits (583), Expect = 2e-59
 Identities = 110/131 (83%), Positives = 125/131 (94%)
 Frame = +2

Query: 89  MTSFLKPENALKRAEELINVGQKQDALQTLHDVITSKRYRAWQKTLERIMFKYVELCVEL 268
           M +F KPENALKRA+ELINVGQKQDALQ LHD+ITSKRYRAWQK LE+IMFKY++LCV+L
Sbjct: 1   MANFAKPENALKRADELINVGQKQDALQALHDLITSKRYRAWQKPLEKIMFKYLDLCVDL 60

Query: 269 RKGRFAKDGLIQYRIICQQVNVSSLEEVIKHFMHLSTEKAEQARSQAQALEEALDVDDLE 448
           ++GRFAKDGLIQYRI+CQQVNVSSLEEVIKHF+HL+T+KAEQARSQA ALEEALDVDDLE
Sbjct: 61  KRGRFAKDGLIQYRIVCQQVNVSSLEEVIKHFLHLATDKAEQARSQADALEEALDVDDLE 120

Query: 449 ADKRPEDLMLS 481
           AD++PEDL LS
Sbjct: 121 ADRKPEDLQLS 131

>dbj|BAB07943.1| putative eukaryotic translation initiation factor 3 large subunit
           [Oryza sativa (japonica cultivar-group)]
          Length = 984

 Score =  228 bits (581), Expect = 3e-59
 Identities = 114/131 (87%), Positives = 123/131 (93%)
 Frame = +2

Query: 89  MTSFLKPENALKRAEELINVGQKQDALQTLHDVITSKRYRAWQKTLERIMFKYVELCVEL 268
           M +F KPENALKRAEELI+VGQKQ ALQ LHD+ITSKRYR+WQK LERIM KYVELCV+L
Sbjct: 1   MATFAKPENALKRAEELIHVGQKQAALQALHDLITSKRYRSWQKPLERIMMKYVELCVDL 60

Query: 269 RKGRFAKDGLIQYRIICQQVNVSSLEEVIKHFMHLSTEKAEQARSQAQALEEALDVDDLE 448
           RKGRFAKDGLIQYRI+CQQVNVSSLEEVIKHFM LS EKAEQAR+QAQALE+ALDV+DLE
Sbjct: 61  RKGRFAKDGLIQYRIVCQQVNVSSLEEVIKHFMQLSNEKAEQARNQAQALEDALDVEDLE 120

Query: 449 ADKRPEDLMLS 481
           ADKRPEDLMLS
Sbjct: 121 ADKRPEDLMLS 131

>sp|Q9XHR2|IF3A_MAIZE Eukaryotic translation initiation factor 3 subunit 10 (eIF-3 theta)
           (Eukaryotic translation initiation factor 3 large
           subunit) (eIF3a) gi|5106764|gb|AAD39834.1|AF073329_1
           eukaryotic translation initiation factor 3 large subunit
           [Zea mays]
          Length = 962

 Score =  224 bits (570), Expect = 5e-58
 Identities = 110/131 (83%), Positives = 122/131 (92%)
 Frame = +2

Query: 89  MTSFLKPENALKRAEELINVGQKQDALQTLHDVITSKRYRAWQKTLERIMFKYVELCVEL 268
           M +F KPENALKRAEELI+VGQKQ ALQ LHD+ITSKRYR+WQK LE+IM KYVELCV+L
Sbjct: 1   MATFAKPENALKRAEELIHVGQKQSALQALHDLITSKRYRSWQKPLEKIMMKYVELCVDL 60

Query: 269 RKGRFAKDGLIQYRIICQQVNVSSLEEVIKHFMHLSTEKAEQARSQAQALEEALDVDDLE 448
           RKGRFAKDGLIQYRI+CQQVNVSSLE+VIKHFM LS EKAEQA+SQ +ALE+ALDV+DLE
Sbjct: 61  RKGRFAKDGLIQYRIVCQQVNVSSLEDVIKHFMQLSNEKAEQAKSQVEALEDALDVEDLE 120

Query: 449 ADKRPEDLMLS 481
           ADKRPEDLMLS
Sbjct: 121 ADKRPEDLMLS 131

>sp|P23116|IF3A_MOUSE Eukaryotic translation initiation factor 3 subunit 10 (eIF-3 theta)
           (eIF3 p167) (eIF3 p180) (eIF3 p185) (eIF3a) (p162
           protein) (Centrosomin) gi|11360364|pir||T42637
           hypothetical protein 162K - mouse
           gi|1205976|gb|AAA90910.1| p162 protein
          Length = 1344

 Score =  140 bits (353), Expect = 7e-33
 Identities = 67/128 (52%), Positives = 95/128 (73%)
 Frame = +2

Query: 98  FLKPENALKRAEELINVGQKQDALQTLHDVITSKRYRAWQKTLERIMFKYVELCVELRKG 277
           F +PENALKRA E + VG+KQ AL  L+DV+ SK++R WQK  E IM KY+ELCV+LRK 
Sbjct: 5   FQRPENALKRANEFLEVGKKQPALDVLYDVMKSKKHRTWQKIHEPIMLKYLELCVDLRKS 64

Query: 278 RFAKDGLIQYRIICQQVNVSSLEEVIKHFMHLSTEKAEQARSQAQALEEALDVDDLEADK 457
             AK+GL QY+ ICQQVN+ SLE+V++ ++ L+ EK E A+ ++Q +   LD++DL+  +
Sbjct: 65  HLAKEGLYQYKNICQQVNIKSLEDVVRAYLKLAEEKTEAAKEESQQM--VLDIEDLDNIQ 122

Query: 458 RPEDLMLS 481
            PE ++LS
Sbjct: 123 TPESVLLS 130

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 435,428,886
Number of Sequences: 1393205
Number of extensions: 9360104
Number of successful extensions: 33750
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 31292
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 33413
length of database: 448,689,247
effective HSP length: 113
effective length of database: 291,257,082
effective search space used: 13689082854
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MFL004b09_f BP033588 1 447 2 GNLf004b11 BP075007 31 483

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