Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC007476A_C01 KMC007476A_c01
(578 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAB09318.1| ubiquitin activating enzyme E1-like protein [Ara... 123 2e-27
ref|NP_568652.1| ubiquitin activating enzyme E1-like protein; pr... 123 2e-27
dbj|BAB17106.1| P0410E01.27 [Oryza sativa (japonica cultivar-gro... 106 2e-22
gb|AAO39077.1| autophagy protein 7 [Dictyostelium discoideum] 89 3e-20
ref|NP_006386.1| ubiquitin activating enzyme E1-like protein [Ho... 87 8e-18
>dbj|BAB09318.1| ubiquitin activating enzyme E1-like protein [Arabidopsis thaliana]
Length = 678
Score = 123 bits (308), Expect = 2e-27
Identities = 69/113 (61%), Positives = 82/113 (72%), Gaps = 2/113 (1%)
Frame = -1
Query: 578 NRTLDQQCTVTRPGLAPIASALAVELLVGILHHPQGILAEADIGN-GVTGAN-EQPLGIL 405
+RTLDQQCTVTRPGLAPIA ALAVELLVG+L HP GI A+ D + TG N + PLGIL
Sbjct: 532 DRTLDQQCTVTRPGLAPIAGALAVELLVGVLQHPLGINAKGDNSSLSNTGNNDDSPLGIL 591
Query: 404 PHQIRGSLSQFSQMNLVGYSSTSCTACCDTRCIQSTETEEWRSYFKQLIILPT 246
PHQIRGS+SQFSQ+ L+G +S SCTAC +T + S E S+ + I PT
Sbjct: 592 PHQIRGSVSQFSQITLLGQASNSCTACSET--VISEYRERGNSFILEAINHPT 642
Score = 70.1 bits (170), Expect = 2e-11
Identities = 34/55 (61%), Positives = 43/55 (77%)
Frame = -3
Query: 315 TLYPEYRNRGMEVVLQAINHPTYLEDLTGLTELMKSVSSFSLDFDKELDDEDEED 151
T+ EYR RG +L+AINHPTYLEDLTGLTEL K+ +SF+LD+ E DD D++D
Sbjct: 621 TVISEYRERGNSFILEAINHPTYLEDLTGLTELKKAANSFNLDW--EDDDTDDDD 673
>ref|NP_568652.1| ubiquitin activating enzyme E1-like protein; protein id:
At5g45900.1, supported by cDNA: gi_19912146 [Arabidopsis
thaliana] gi|14334508|gb|AAK59451.1| putative ubiquitin
activating enzyme E1 [Arabidopsis thaliana]
gi|19912147|dbj|BAB88385.1| autophagy 7 [Arabidopsis
thaliana] gi|21636960|gb|AAM70190.1|AF492761_1 autophagy
APG7 [Arabidopsis thaliana] gi|23297572|gb|AAN12897.1|
putative ubiquitin-activating enzyme E1 [Arabidopsis
thaliana]
Length = 697
Score = 123 bits (308), Expect = 2e-27
Identities = 69/113 (61%), Positives = 82/113 (72%), Gaps = 2/113 (1%)
Frame = -1
Query: 578 NRTLDQQCTVTRPGLAPIASALAVELLVGILHHPQGILAEADIGN-GVTGAN-EQPLGIL 405
+RTLDQQCTVTRPGLAPIA ALAVELLVG+L HP GI A+ D + TG N + PLGIL
Sbjct: 551 DRTLDQQCTVTRPGLAPIAGALAVELLVGVLQHPLGINAKGDNSSLSNTGNNDDSPLGIL 610
Query: 404 PHQIRGSLSQFSQMNLVGYSSTSCTACCDTRCIQSTETEEWRSYFKQLIILPT 246
PHQIRGS+SQFSQ+ L+G +S SCTAC +T + S E S+ + I PT
Sbjct: 611 PHQIRGSVSQFSQITLLGQASNSCTACSET--VISEYRERGNSFILEAINHPT 661
Score = 70.1 bits (170), Expect = 2e-11
Identities = 34/55 (61%), Positives = 43/55 (77%)
Frame = -3
Query: 315 TLYPEYRNRGMEVVLQAINHPTYLEDLTGLTELMKSVSSFSLDFDKELDDEDEED 151
T+ EYR RG +L+AINHPTYLEDLTGLTEL K+ +SF+LD+ E DD D++D
Sbjct: 640 TVISEYRERGNSFILEAINHPTYLEDLTGLTELKKAANSFNLDW--EDDDTDDDD 692
>dbj|BAB17106.1| P0410E01.27 [Oryza sativa (japonica cultivar-group)]
Length = 800
Score = 106 bits (265), Expect = 2e-22
Identities = 56/111 (50%), Positives = 73/111 (65%)
Frame = -1
Query: 578 NRTLDQQCTVTRPGLAPIASALAVELLVGILHHPQGILAEADIGNGVTGANEQPLGILPH 399
NRTLDQQCTVTRPGL+ I S A +L +LHHP GI A +I ++E PLG+LPH
Sbjct: 661 NRTLDQQCTVTRPGLSSITSGCAADLFTRMLHHPDGIHAPGEIAG---TSSEGPLGLLPH 717
Query: 398 QIRGSLSQFSQMNLVGYSSTSCTACCDTRCIQSTETEEWRSYFKQLIILPT 246
QIRGSLSQ++ + L+GYSS++CTAC + + S + Q+I PT
Sbjct: 718 QIRGSLSQYNLLTLLGYSSSNCTAC--SNAVLSEYHRRGMDFVMQVINEPT 766
Score = 72.0 bits (175), Expect = 5e-12
Identities = 45/111 (40%), Positives = 58/111 (51%)
Frame = -1
Query: 578 NRTLDQQCTVTRPGLAPIASALAVELLVGILHHPQGILAEADIGNGVTGANEQPLGILPH 399
N LDQQ V PGL +AS AVEL +LHHP I A DI T E LG+LPH
Sbjct: 317 NGALDQQSAVILPGLTSVASGKAVELFARMLHHPDEIHAPGDIAGTDT---EHQLGLLPH 373
Query: 398 QIRGSLSQFSQMNLVGYSSTSCTACCDTRCIQSTETEEWRSYFKQLIILPT 246
Q++GSLS+ ++ SS++C AC + + S + Q I PT
Sbjct: 374 QMQGSLSKCVLSTVLCNSSSNCIAC--SNAVLSEYRRRGFDFVTQAITCPT 422
Score = 67.8 bits (164), Expect = 9e-11
Identities = 40/92 (43%), Positives = 52/92 (56%), Gaps = 19/92 (20%)
Frame = -3
Query: 369 SNEPCRLLLHQLHG---------LLRYT----------LYPEYRNRGMEVVLQAINHPTY 247
S P LL HQ+ G LL Y+ + EY RGM+ V+Q IN PTY
Sbjct: 708 SEGPLGLLPHQIRGSLSQYNLLTLLGYSSSNCTACSNAVLSEYHRRGMDFVMQVINEPTY 767
Query: 246 LEDLTGLTELMKSVSSFSLDFDKELDDEDEED 151
LEDLTGLT+LMKS + +++ E+DD+DE D
Sbjct: 768 LEDLTGLTDLMKSAAYSQVEWIDEVDDDDEMD 799
Score = 44.3 bits (103), Expect = 0.001
Identities = 20/35 (57%), Positives = 26/35 (74%)
Frame = -3
Query: 303 EYRNRGMEVVLQAINHPTYLEDLTGLTELMKSVSS 199
EYR RG + V QAI PTYL+DLTG+++L K +S
Sbjct: 405 EYRRRGFDFVTQAITCPTYLKDLTGISDLKKPFAS 439
>gb|AAO39077.1| autophagy protein 7 [Dictyostelium discoideum]
Length = 707
Score = 89.0 bits (219), Expect(2) = 3e-20
Identities = 42/87 (48%), Positives = 56/87 (64%)
Frame = -1
Query: 578 NRTLDQQCTVTRPGLAPIASALAVELLVGILHHPQGILAEADIGNGVTGANEQPLGILPH 399
+RTLDQ CTVTRPGL+ +AS++AVELL+ +HHP G A+ + V PLGI+PH
Sbjct: 556 DRTLDQMCTVTRPGLSMMASSIAVELLISTIHHPYGGRAKGETETDVYVQGSTPLGIIPH 615
Query: 398 QIRGSLSQFSQMNLVGYSSTSCTACCD 318
Q+RG +S + + L CTAC D
Sbjct: 616 QLRGFISHYQTLPLFSNPYKHCTACSD 642
Score = 31.2 bits (69), Expect(2) = 3e-20
Identities = 11/51 (21%), Positives = 31/51 (60%)
Frame = -3
Query: 303 EYRNRGMEVVLQAINHPTYLEDLTGLTELMKSVSSFSLDFDKELDDEDEED 151
EY ++G + ++ +N + L + G+ +L + ++D+D ++ D+D+++
Sbjct: 647 EYNSKGFDFIINVMNDSSCLTKICGIDDLKN--TEVNIDWDIDISDDDDDN 695
>ref|NP_006386.1| ubiquitin activating enzyme E1-like protein [Homo sapiens]
gi|3820614|gb|AAC69630.1| E1-like protein [Homo sapiens]
Length = 703
Score = 87.4 bits (215), Expect(2) = 8e-18
Identities = 45/85 (52%), Positives = 56/85 (64%)
Frame = -1
Query: 578 NRTLDQQCTVTRPGLAPIASALAVELLVGILHHPQGILAEADIGNGVTGANEQPLGILPH 399
+RTLDQQCTV+RPGLA IA ALAVEL+V +L HP+G A A + LG++PH
Sbjct: 565 DRTLDQQCTVSRPGLAVIAGALAVELMVSVLQHPEGGYAIASSSDDRMNEPPTSLGLVPH 624
Query: 398 QIRGSLSQFSQMNLVGYSSTSCTAC 324
QIRG LS+F + V + CTAC
Sbjct: 625 QIRGFLSRFDNVLPVSLAFDKCTAC 649
Score = 24.3 bits (51), Expect(2) = 8e-18
Identities = 12/30 (40%), Positives = 17/30 (56%), Gaps = 1/30 (3%)
Frame = -3
Query: 303 EYRNRGMEVVLQAINHP-TYLEDLTGLTEL 217
+Y G + + N ++LEDLTGLT L
Sbjct: 656 QYEREGFNFLAKVFNSSHSFLEDLTGLTLL 685
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 474,882,084
Number of Sequences: 1393205
Number of extensions: 9716698
Number of successful extensions: 52120
Number of sequences better than 10.0: 52
Number of HSP's better than 10.0 without gapping: 32054
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 42675
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 21426319650
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
