Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC007029A_C01 KMC007029A_c01
(600 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAM12534.1|AF466378_1 putative sphingolipid delta 4 desaturas... 275 3e-73
ref|NP_192402.1| putative fatty acid desaturase; protein id: At4... 263 2e-69
ref|NP_476594.1| infertile crescent CG9078-PA gi|1667359|emb|CAA... 178 4e-44
ref|NP_031879.1| degenerative spermatocyte homolog (Drosophila) ... 175 3e-43
gb|AAM12532.1|AF466376_1 sphingolipid delta 4 desaturase protein... 175 5e-43
>gb|AAM12534.1|AF466378_1 putative sphingolipid delta 4 desaturase DES-1 [Lycopersicon
esculentum]
Length = 332
Score = 275 bits (703), Expect = 3e-73
Identities = 121/138 (87%), Positives = 133/138 (95%)
Frame = -1
Query: 600 KPKPPGIWEFINFSVQIALDVAMVYFWGWKSLAYLILSTFVGGGMHPMAGHFISEHYVFN 421
KPKPPG+WEFINF++Q++LD AMVYFWGWKSLAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 186 KPKPPGLWEFINFTIQLSLDGAMVYFWGWKSLAYLILSTFVGGGMHPMAGHFISEHYVFK 245
Query: 420 PDQETYSYYGPLNLVTWHVGYHNEHHDFPRIPGNKLHKVKEIAPEYYDELASYKSWSQVI 241
P+QETYSYYGPLNL+TW VGYHNEHHDFPRIPG+KL+KVKEIAPEYY+ L +YKSWSQVI
Sbjct: 246 PEQETYSYYGPLNLMTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYENLDAYKSWSQVI 305
Query: 240 YMYLMDRTVGPFSRMKRK 187
YMY+MDRTVGPFSRMKRK
Sbjct: 306 YMYIMDRTVGPFSRMKRK 323
>ref|NP_192402.1| putative fatty acid desaturase; protein id: At4g04930.1, supported
by cDNA: gi_6752877 [Arabidopsis thaliana]
gi|25353107|pir||A85062 probable fatty acid desaturase
[imported] - Arabidopsis thaliana
gi|4325341|gb|AAD17340.1| similar to the Drosophila
DES-1 protein (GB:X94180) [Arabidopsis thaliana]
gi|6752878|gb|AAF27915.1|AF220201_1 DES-1-like
transmembrane protein [Arabidopsis thaliana]
gi|7267252|emb|CAB81035.1| putative fatty acid
desaturase [Arabidopsis thaliana]
Length = 332
Score = 263 bits (671), Expect = 2e-69
Identities = 118/143 (82%), Positives = 130/143 (90%)
Frame = -1
Query: 600 KPKPPGIWEFINFSVQIALDVAMVYFWGWKSLAYLILSTFVGGGMHPMAGHFISEHYVFN 421
KPKPPG WEFINF +QI LDV++V F+GW+S AYLILSTFVGGGMHPMAGHFISEHYVFN
Sbjct: 190 KPKPPGYWEFINFLIQIVLDVSVVLFFGWRSFAYLILSTFVGGGMHPMAGHFISEHYVFN 249
Query: 420 PDQETYSYYGPLNLVTWHVGYHNEHHDFPRIPGNKLHKVKEIAPEYYDELASYKSWSQVI 241
P+QETYSYYGPLNL+TW VGYHNEHHDFPRIPGNKLH VKEIA EYY+ L SYKSWSQVI
Sbjct: 250 PNQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLHLVKEIAGEYYEGLESYKSWSQVI 309
Query: 240 YMYLMDRTVGPFSRMKRKPGKTE 172
YMY+MD TVGP+SRMKRK K++
Sbjct: 310 YMYIMDTTVGPYSRMKRKLSKSD 332
>ref|NP_476594.1| infertile crescent CG9078-PA gi|1667359|emb|CAA63889.1| Des-1
protein [Drosophila melanogaster]
gi|7297049|gb|AAF52318.1| CG9078-PA [Drosophila
melanogaster] gi|16769050|gb|AAL28744.1| LD15458p
[Drosophila melanogaster]
gi|20147522|gb|AAM12535.1|AF466379_1 sphingolipid delta
4 desaturase protein DES-1 [Drosophila melanogaster]
Length = 321
Score = 178 bits (452), Expect = 4e-44
Identities = 76/137 (55%), Positives = 105/137 (76%)
Frame = -1
Query: 597 PKPPGIWEFINFSVQIALDVAMVYFWGWKSLAYLILSTFVGGGMHPMAGHFISEHYVFNP 418
PKPP E IN VQ+ + +VYF GWK LAYL++ + + G+HP+AGHFISEHY+F
Sbjct: 179 PKPPTRLEIINTVVQLTFNALIVYFLGWKPLAYLLIGSILAMGLHPVAGHFISEHYMFAK 238
Query: 417 DQETYSYYGPLNLVTWHVGYHNEHHDFPRIPGNKLHKVKEIAPEYYDELASYKSWSQVIY 238
ETYSYYGPLN +T++VGYHNEHHDFP +PG++L +VK IA E+YD + + SW++V+Y
Sbjct: 239 GFETYSYYGPLNWITFNVGYHNEHHDFPAVPGSRLPEVKRIAKEFYDTMPQHTSWTRVLY 298
Query: 237 MYLMDRTVGPFSRMKRK 187
++MD VGP++R+KR+
Sbjct: 299 DFIMDPAVGPYARVKRR 315
>ref|NP_031879.1| degenerative spermatocyte homolog (Drosophila) [Mus musculus]
gi|2225941|emb|CAA69714.1| Mdes protein [Mus musculus]
gi|12832732|dbj|BAB22233.1| unnamed protein product [Mus
musculus] gi|13277699|gb|AAH03751.1| degenerative
spermatocyte homolog (Drosophila) [Mus musculus]
gi|26346124|dbj|BAC36713.1| unnamed protein product [Mus
musculus]
Length = 323
Score = 175 bits (444), Expect = 3e-43
Identities = 77/142 (54%), Positives = 103/142 (72%)
Frame = -1
Query: 597 PKPPGIWEFINFSVQIALDVAMVYFWGWKSLAYLILSTFVGGGMHPMAGHFISEHYVFNP 418
PKP E IN +QI D+ + Y +G KSL Y++ +T +G G+HP++GHFI+EHY+F
Sbjct: 179 PKPITYLEIINTVIQITFDIIIYYVFGVKSLVYMLAATLLGLGLHPISGHFIAEHYMFLK 238
Query: 417 DQETYSYYGPLNLVTWHVGYHNEHHDFPRIPGNKLHKVKEIAPEYYDELASYKSWSQVIY 238
ETYSYYGPLNL+T++VGYHNEHHDFP +PG L V++IA EYYD+L Y SW +V+Y
Sbjct: 239 GHETYSYYGPLNLLTFNVGYHNEHHDFPNVPGKNLPMVRKIASEYYDDLPHYNSWIKVLY 298
Query: 237 MYLMDRTVGPFSRMKRKPGKTE 172
++ D T+ P+SRMKR P E
Sbjct: 299 DFVTDDTISPYSRMKRPPKGNE 320
>gb|AAM12532.1|AF466376_1 sphingolipid delta 4 desaturase protein DES1 [Mus musculus]
Length = 323
Score = 175 bits (443), Expect = 5e-43
Identities = 77/142 (54%), Positives = 103/142 (72%)
Frame = -1
Query: 597 PKPPGIWEFINFSVQIALDVAMVYFWGWKSLAYLILSTFVGGGMHPMAGHFISEHYVFNP 418
PKP E IN +QI D+ + Y +G KSL Y++ +T +G G+HP++GHFI+EHY+F
Sbjct: 179 PKPITYLEIINTVIQITFDIIIYYAFGVKSLVYMLAATLLGLGLHPISGHFIAEHYMFLK 238
Query: 417 DQETYSYYGPLNLVTWHVGYHNEHHDFPRIPGNKLHKVKEIAPEYYDELASYKSWSQVIY 238
ETYSYYGPLNL+T++VGYHNEHHDFP +PG L V++IA EYYD+L Y SW +V+Y
Sbjct: 239 GHETYSYYGPLNLLTFNVGYHNEHHDFPNVPGKNLPMVRKIASEYYDDLPHYNSWIKVLY 298
Query: 237 MYLMDRTVGPFSRMKRKPGKTE 172
++ D T+ P+SRMKR P E
Sbjct: 299 DFVTDDTISPYSRMKRPPKGNE 320
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 567,576,702
Number of Sequences: 1393205
Number of extensions: 13090482
Number of successful extensions: 32767
Number of sequences better than 10.0: 65
Number of HSP's better than 10.0 without gapping: 31828
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 32752
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 23426109484
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
