Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC006753A_C01 KMC006753A_c01
(523 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_565330.1| subtilisin-like serine protease, putative; prot... 157 9e-38
gb|AAK25839.1|AF360129_1 putative subtilisin serine protease [Ar... 145 3e-34
ref|NP_566483.1| subtilisin-like serine protease; protein id: At... 145 4e-34
gb|AAM60964.1| subtilisin-like serine protease [Arabidopsis thal... 143 1e-33
pir||T07172 subtilisin-like proteinase (EC 3.4.21.-) 2 - tomato ... 136 1e-31
>ref|NP_565330.1| subtilisin-like serine protease, putative; protein id: At2g05920.1,
supported by cDNA: gi_14334833 [Arabidopsis thaliana]
gi|25289829|pir||A84473 probable serine proteinase
[imported] - Arabidopsis thaliana
gi|4006827|gb|AAC95169.1| subtilisin-like serine
protease, putative [Arabidopsis thaliana]
gi|14334834|gb|AAK59595.1| putative subtilisin serine
protease [Arabidopsis thaliana]
gi|23296838|gb|AAN13182.1| putative subtilisin serine
protease [Arabidopsis thaliana]
Length = 754
Score = 157 bits (396), Expect = 9e-38
Identities = 83/173 (47%), Positives = 111/173 (63%), Gaps = 1/173 (0%)
Frame = +2
Query: 8 TLSFTFILLLLQCLSASCSSPKKTYIVHMNHHTKPQIYPTRRDWYTASLRSLSLTTDSET 187
T+ TF+ LLL ++ KKTYI+ +NH KP+ + T DWYT+ L S
Sbjct: 12 TIITTFLFLLLH------TTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNS--------- 56
Query: 188 SDDPLLYAYDTAYNGFAASLDEQQAQTLLGS-DSVLGLYEDTLYHLHTTRTPQFLGL*HT 364
+ LLY Y T+++GF+A LD +A +LL S +S+L ++ED LY LHTTRTP+FLGL
Sbjct: 57 -ESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGL--- 112
Query: 365 NGGCGKDIELLELDQASRDVIIGVLDTGVWPESPSFNDAGMPEIPSRWRGECE 523
+ + +L +S VIIGVLDTGVWPES SF+D MPEIPS+W+GECE
Sbjct: 113 ----NSEFGVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECE 161
>gb|AAK25839.1|AF360129_1 putative subtilisin serine protease [Arabidopsis thaliana]
Length = 775
Score = 145 bits (366), Expect = 3e-34
Identities = 75/170 (44%), Positives = 105/170 (61%), Gaps = 1/170 (0%)
Frame = +2
Query: 14 SFTFILLLLQCLSASCSSPKK-TYIVHMNHHTKPQIYPTRRDWYTASLRSLSLTTDSETS 190
S+ F LL L S+S SS TYIVH++H KP I+PT WYT+SL SL+ +S
Sbjct: 5 SYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLT------SS 58
Query: 191 DDPLLYAYDTAYNGFAASLDEQQAQTLLGSDSVLGLYEDTLYHLHTTRTPQFLGL*HTNG 370
+++ YDT ++GF+A L Q A LL V+ + + + HLHTTR+P+FLGL T+
Sbjct: 59 PPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDK 118
Query: 371 GCGKDIELLELDQASRDVIIGVLDTGVWPESPSFNDAGMPEIPSRWRGEC 520
LLE D++IGV+DTGVWPE PSF+D G+ +P +W+G+C
Sbjct: 119 A-----GLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQC 163
>ref|NP_566483.1| subtilisin-like serine protease; protein id: At3g14240.1, supported
by cDNA: 106539., supported by cDNA: gi_13430433,
supported by cDNA: gi_16930700 [Arabidopsis thaliana]
gi|9279572|dbj|BAB01030.1| subtilisin proteinase-like
protein [Arabidopsis thaliana]
Length = 775
Score = 145 bits (365), Expect = 4e-34
Identities = 73/168 (43%), Positives = 104/168 (61%)
Frame = +2
Query: 17 FTFILLLLQCLSASCSSPKKTYIVHMNHHTKPQIYPTRRDWYTASLRSLSLTTDSETSDD 196
F F+L L S++ SS TYIVH++H KP I+PT WYT+SL SL+ +S
Sbjct: 7 FFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLT------SSPP 60
Query: 197 PLLYAYDTAYNGFAASLDEQQAQTLLGSDSVLGLYEDTLYHLHTTRTPQFLGL*HTNGGC 376
+++ YDT ++GF+A L Q A LL V+ + + + HLHTTR+P+FLGL T+
Sbjct: 61 SIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKA- 119
Query: 377 GKDIELLELDQASRDVIIGVLDTGVWPESPSFNDAGMPEIPSRWRGEC 520
LLE D++IGV+DTGVWPE PSF+D G+ +P +W+G+C
Sbjct: 120 ----GLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQC 163
>gb|AAM60964.1| subtilisin-like serine protease [Arabidopsis thaliana]
Length = 775
Score = 143 bits (361), Expect = 1e-33
Identities = 72/168 (42%), Positives = 104/168 (61%)
Frame = +2
Query: 17 FTFILLLLQCLSASCSSPKKTYIVHMNHHTKPQIYPTRRDWYTASLRSLSLTTDSETSDD 196
F F+L L S++ SS TYIVH++H KP I+PT WYT+SL SL+ +S
Sbjct: 7 FFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHLHWYTSSLASLT------SSPP 60
Query: 197 PLLYAYDTAYNGFAASLDEQQAQTLLGSDSVLGLYEDTLYHLHTTRTPQFLGL*HTNGGC 376
+++ Y+T ++GF+A L Q A LL V+ + + + HLHTTR+P+FLGL T+
Sbjct: 61 SIIHTYNTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKA- 119
Query: 377 GKDIELLELDQASRDVIIGVLDTGVWPESPSFNDAGMPEIPSRWRGEC 520
LLE D++IGV+DTGVWPE PSF+D G+ +P +W+G+C
Sbjct: 120 ----GLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQC 163
>pir||T07172 subtilisin-like proteinase (EC 3.4.21.-) 2 - tomato
gi|1771162|emb|CAA67430.1| SBT2 [Lycopersicon
esculentum] gi|3687307|emb|CAA07000.1| subtilisin-like
protease [Lycopersicon esculentum]
Length = 775
Score = 136 bits (343), Expect = 1e-31
Identities = 75/175 (42%), Positives = 108/175 (60%), Gaps = 6/175 (3%)
Frame = +2
Query: 17 FTFILLLLQCLSASCS-SPKKTYIVHMNHHTKPQIYPTRRDWYTASLRS-LSLTTDSETS 190
F F+ + L A CS + KKTYI+ M+ KP ++ WY++ ++S L TT+ E +
Sbjct: 10 FFFVSVCLAINLAKCSPNTKKTYIIQMDKWAKPDVFVDHVQWYSSLVKSVLPSTTEVEKT 69
Query: 191 DDP---LLYAYDTAYNGFAASLDEQQAQTLLGSDSVLGLYEDTLYHLHTTRTPQFLGL*H 361
D +LY+Y TA++G AA L E++ + L + VL ++ + Y LHTTR+P FLGL
Sbjct: 70 GDGEERILYSYQTAFHGVAAQLSEEEVKKLQERNGVLAVFPEIKYQLHTTRSPLFLGLDR 129
Query: 362 TNGGCGKDIELLELDQAS-RDVIIGVLDTGVWPESPSFNDAGMPEIPSRWRGECE 523
+D L D+ S +VI+GVLDTG+WPESPSFND+GM +PS W+G CE
Sbjct: 130 ------EDSSKLWADRLSDHNVIVGVLDTGIWPESPSFNDSGMTSVPSHWKGVCE 178
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 560,409,014
Number of Sequences: 1393205
Number of extensions: 15265219
Number of successful extensions: 87216
Number of sequences better than 10.0: 1244
Number of HSP's better than 10.0 without gapping: 62744
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 80584
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 16731298976
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
