>KMC006542A_C01 KMC006542A_c01
gcatagagcacaacatcaaattcgacggtaagtcataagatttcaacagaccaaaagatc
gcacttaggaacataaaagaGTTTTAAAAATGTGCTGAGTACTGCATATCAAAACACTGG
TATCATGGGTCCATCCAAACAACAATCCCAGAACATGGTTTATGAAAAAGAAAGTTGAAG
CATTATCAGCAACCCCTTCAATTTCAAAGCTCATAGAGAAGTCCAAATTATATCAACGAT
AGCCTCCTGATGATCCCCTTCGTTCATACGGTCCAGAACGATCACGATGGTATCGATCAC
TTCCAGAATCTCCATGACTACCACCATCCCTGCTGCTGCGCCCACCAGAAGAACGATCTG
CATTTCTATCAGGACCATAGCCACCGCCACTGnTTCGACTTTCCCGGCCACCATACCTTC
CCCCTCCTCGTTCCCCTTCACTAGGACACTCCCTAGCAAAATGACCAGGCTTACCACACT
TAAAGCATTCACCTCCATTAGATCCACGACTACCTCCATAGCCTCGGCTACGATCACGAT
CGCCACGATCATCACGATCACGACCACGCTCTCTGTAGCGATCACCATCATCCCTATCCC
TAGATGATCCTTGTGGCTGAGCTTTATCAACAGTAATAGTACGCCCATCTAAATCCATCC
CATTCATAGCATCAATAGCCTCGTCCATTGCTTTCTTGTCATCAAATGTGACAAATCCAA
ACCCCCGAGAGCGTCCAGAGAACTTGTCAACAACAACCTTTGCTTCCGTAAGCTTGCCAA
ACTTTTCAAATGCATCCTTTAACTTTCTATCAGATGTTGACCAGGcaggccaccaataaa
acagcgatactcgtccacatctgacatcttaaacgattcggaggtggagttgttggagaa
aaggc

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC006542A_C01 KMC006542A_c01
         (905 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

pir||JC4817 RNA-binding protein RZ-1 - wood tobacco gi|1395193|d...   263  4e-70
ref|NP_189273.1| glycine-rich RNA-binding protein; protein id: A...   220  6e-58
gb|AAL09710.1| AT3g26420/F20C19_14 [Arabidopsis thaliana] gi|196...   191  2e-49
gb|AAK01176.1| RNA-binding protein [Triticum aestivum]                195  9e-49
ref|NP_196048.1| glycine-rich RNA-binding protein; protein id: A...   129  8e-29

>pir||JC4817 RNA-binding protein RZ-1 - wood tobacco gi|1395193|dbj|BAA12064.1|
           RNA-binding protein RZ-1 [Nicotiana sylvestris]
           gi|1435062|dbj|BAA06012.1| RNA binding protein, RZ-1
           [Nicotiana sylvestris]
          Length = 209

 Score =  263 bits (673), Expect(2) = 4e-70
 Identities = 142/200 (71%), Positives = 156/200 (78%), Gaps = 3/200 (1%)
 Frame = -2

Query: 826 AWSTSDRKLKDAFEKFGKLTEAKVVVDKFSGRSRGFGFVTFDDKKAMDEAIDAMNGMDLD 647
           +WSTSDR LKDAFEKFG L +AKVV+DKFSGRSRGFGFVTFD+K+AM++AI+AMNG+DLD
Sbjct: 14  SWSTSDRGLKDAFEKFGNLVDAKVVLDKFSGRSRGFGFVTFDEKRAMEDAIEAMNGVDLD 73

Query: 646 GRTITVDKAQP-QGSSRDRDDGDRYRERGRDRDDRGDRDR-SRGYGGSRGS-NGGECFKC 476
           GR ITVDKAQP +GS RD  D DR R+R RDR    DRDR SR YGG RGS  GG+CF C
Sbjct: 74  GRDITVDKAQPDKGSGRD-FDSDRPRDRDRDRGRDRDRDRGSRDYGGGRGSGGGGDCFNC 132

Query: 475 GKPGHFARECPSEGERGGGRYGGRESRXSGGGYGPDRNADRSSGGRSSRDGGSHGDSGSD 296
           GKPGHFARECPSEG R GGRYGG        GYGPDRN DR  G RS RDGG  G  G +
Sbjct: 133 GKPGHFARECPSEGGR-GGRYGGGGGGSRSSGYGPDRNGDR-YGSRSGRDGGGRG--GGE 188

Query: 295 RYHRDRSGPYERRGSSGGYR 236
           R+ RDRSGPYERR SSGG R
Sbjct: 189 RFSRDRSGPYERR-SSGGSR 207

 Score = 24.6 bits (52), Expect(2) = 4e-70
 Identities = 9/10 (90%), Positives = 9/10 (90%)
 Frame = -3

Query: 855 DEYRCFIGGL 826
           DEYRCFIG L
Sbjct: 4   DEYRCFIGNL 13

>ref|NP_189273.1| glycine-rich RNA-binding protein; protein id: At3g26420.1,
           supported by cDNA: gi_15451065, supported by cDNA:
           gi_15982738, supported by cDNA: gi_15983476, supported
           by cDNA: gi_18377411 [Arabidopsis thaliana]
           gi|9294301|dbj|BAB02203.1| contains similarity to
           RNA-binding protein~gene_id:F20C19.15 [Arabidopsis
           thaliana] gi|15451066|gb|AAK96804.1| Unknown protein
           [Arabidopsis thaliana]
           gi|15983477|gb|AAL11606.1|AF424613_1 AT3g26420/F20C19_14
           [Arabidopsis thaliana] gi|18377412|gb|AAL66872.1|
           unknown protein [Arabidopsis thaliana]
          Length = 245

 Score =  220 bits (560), Expect(2) = 6e-58
 Identities = 133/243 (54%), Positives = 152/243 (61%), Gaps = 49/243 (20%)
 Frame = -2

Query: 826 AWSTSDRKLKDAFEKFGKLTEAKVVVDKFSGRSRGFGFVTFDDKKAMDEAIDAMNGMDLD 647
           AW+TSDR L+DAFEK+G L EAKVV+DKFSGRSRGFGF+TFD+KKAMDEAI AMNGMDLD
Sbjct: 15  AWTTSDRGLRDAFEKYGHLVEAKVVLDKFSGRSRGFGFITFDEKKAMDEAIAAMNGMDLD 74

Query: 646 GRTITVDKAQP-QGSSRDRDDGDRYRERGRDRDDRGDRDRSRGYGGSRGSNGGECFKCGK 470
           GRTITVDKAQP QG +   +DGDR R+RG       DRDRSR  GG RG  GG+CFKCGK
Sbjct: 75  GRTITVDKAQPHQGGAGRDNDGDRGRDRGY------DRDRSRPSGG-RG--GGDCFKCGK 125

Query: 469 PGHFARECPSEGERGGG-----------------------------RYGGRESRXS---- 389
           PGHFARECPSE  R GG                             RYG ++ R S    
Sbjct: 126 PGHFARECPSESSRDGGGRFSSKDDRYSSKDDRYGAKDDRYGAKEDRYGAKDDRYSSKDD 185

Query: 388 -------------GGG--YGPDRNADRSSGGRSSRDGGSHGDSGSDRYHRDRSGPYERRG 254
                        GGG  YGPDR+ +R +GGR SRDGGS G  G +R+ R    PY+R  
Sbjct: 186 RYSSKDDRYGSRDGGGSRYGPDRSGER-AGGR-SRDGGSRGAPGGERHSR---APYDRPR 240

Query: 253 SSG 245
           + G
Sbjct: 241 AGG 243

 Score = 27.3 bits (59), Expect(2) = 6e-58
 Identities = 11/14 (78%), Positives = 12/14 (85%)
 Frame = -3

Query: 867 MSDVDEYRCFIGGL 826
           MS+  EYRCFIGGL
Sbjct: 1   MSEDPEYRCFIGGL 14

>gb|AAL09710.1| AT3g26420/F20C19_14 [Arabidopsis thaliana]
           gi|19699180|gb|AAL90956.1| AT3g26420/F20C19_14
           [Arabidopsis thaliana]
          Length = 148

 Score =  191 bits (486), Expect(2) = 2e-49
 Identities = 100/134 (74%), Positives = 110/134 (81%), Gaps = 1/134 (0%)
 Frame = -2

Query: 826 AWSTSDRKLKDAFEKFGKLTEAKVVVDKFSGRSRGFGFVTFDDKKAMDEAIDAMNGMDLD 647
           AW+TSDR L+DAFEK+G L EAKVV+DKFSGRSRGFGF+TFD+KKAMDEAI AMNGMDLD
Sbjct: 15  AWTTSDRGLRDAFEKYGHLVEAKVVLDKFSGRSRGFGFITFDEKKAMDEAIAAMNGMDLD 74

Query: 646 GRTITVDKAQP-QGSSRDRDDGDRYRERGRDRDDRGDRDRSRGYGGSRGSNGGECFKCGK 470
           GRTITVDKAQP QG +   +DGDR R+RG       DRDRSR  GG RG  GG+CFKCGK
Sbjct: 75  GRTITVDKAQPHQGGAGRDNDGDRGRDRGY------DRDRSRPSGG-RG--GGDCFKCGK 125

Query: 469 PGHFARECPSEGER 428
           PGHFARECPSE  R
Sbjct: 126 PGHFARECPSESSR 139

 Score = 27.3 bits (59), Expect(2) = 2e-49
 Identities = 11/14 (78%), Positives = 12/14 (85%)
 Frame = -3

Query: 867 MSDVDEYRCFIGGL 826
           MS+  EYRCFIGGL
Sbjct: 1   MSEDPEYRCFIGGL 14

>gb|AAK01176.1| RNA-binding protein [Triticum aestivum]
          Length = 183

 Score =  195 bits (495), Expect = 9e-49
 Identities = 111/181 (61%), Positives = 128/181 (70%)
 Frame = -2

Query: 868 DVRCGRVSLFYWWPAWSTSDRKLKDAFEKFGKLTEAKVVVDKFSGRSRGFGFVTFDDKKA 689
           D RC   SL     +W+T+D  LKDAF KFG++TE KVV+DKFSGRSRGFGFVTFDDKKA
Sbjct: 6   DYRCFVGSL-----SWNTTDVDLKDAFGKFGRVTETKVVLDKFSGRSRGFGFVTFDDKKA 60

Query: 688 MDEAIDAMNGMDLDGRTITVDKAQPQGSSRDRDDGDRYRERGRDRDDRGDRDRSRGYGGS 509
           M+EA++AMNG+DLDGR ITV++AQPQGS R+R DGDR    G DR   G RD   G GG 
Sbjct: 61  MEEAVEAMNGIDLDGRNITVERAQPQGSGRNR-DGDRDYRGGGDRYG-GGRDFGGGRGGG 118

Query: 508 RGSNGGECFKCGKPGHFARECPSEGERGGGRYGGRESRXSGGGYGPDRNADRSSGGRSSR 329
           RG  GG+C+KCGKPGHFARECPS    GG RYG R+ R S      DR + R     SSR
Sbjct: 119 RG-GGGDCYKCGKPGHFARECPSGD--GGDRYGSRDDRYSS---RDDRYSSRDD-RYSSR 171

Query: 328 D 326
           D
Sbjct: 172 D 172

>ref|NP_196048.1| glycine-rich RNA-binding protein; protein id: At5g04280.1,
           supported by cDNA: gi_20260151 [Arabidopsis thaliana]
           gi|20260152|gb|AAM12974.1| RNA-binding protein-like
           [Arabidopsis thaliana] gi|21387121|gb|AAM47964.1|
           RNA-binding protein-like [Arabidopsis thaliana]
          Length = 310

 Score =  129 bits (323), Expect = 8e-29
 Identities = 73/162 (45%), Positives = 101/162 (62%), Gaps = 8/162 (4%)
 Frame = -2

Query: 814 SDRKLKDAFEKFGKLTEAKVVVDKFSGRSRGFGFVTFDDKKAMDEAIDAMNGMDLDGRTI 635
           +DR L+ AF +FG + + ++++++ +GRSRGFGF+TF D++AMDE+I  M+G D   R I
Sbjct: 19  TDRDLERAFSRFGDILDCQIMLERDTGRSRGFGFITFADRRAMDESIREMHGRDFGDRVI 78

Query: 634 TVDKAQPQGSSRDRDDGDRYRERGRDRDDRGDRDRSRGYGGSRGSNGG-----ECFKCGK 470
           +V++A+P+     RDDG+ +  RG    D G     +G  G  G  GG     ECFKCG+
Sbjct: 79  SVNRAEPK---LGRDDGESHGSRG--GRDSGYSIAGKGSFGGGGGGGGRVGEDECFKCGR 133

Query: 469 PGHFARECPSEGERGGGRYGGRESRXS--GGGYG-PDRNADR 353
            GH+AR+CPS G   GG  GG  SR S  GG  G  DR ADR
Sbjct: 134 VGHWARDCPSAGGGRGGPVGGFSSRASAYGGSDGRVDRYADR 175

 Score = 42.7 bits (99), Expect = 0.007
 Identities = 43/133 (32%), Positives = 54/133 (40%), Gaps = 17/133 (12%)
 Frame = -2

Query: 589 DGDRYRERGRDRDDRGDRDRSRGYGGSRGSNGGECFKCGKPGHFARE---CPSEGERGGG 419
           D DRY +R R  DDR   D +  YG     +  E +       +A +    P++   GG 
Sbjct: 174 DRDRYVDRERYIDDR--YDGAARYGARDRFDSREAYI--PRDRYASDRYAAPADRFAGGD 229

Query: 418 RYGGRESRXSGGGY----------GPDRNADRSSGGRSS---RDGGSHGDSGSDRYHRDR 278
           RY     R   G Y           P   +DR  GGR+    R GG  G     R  R R
Sbjct: 230 RYSRGSDRYPPGSYDKARSFERDIAPSAGSDRYGGGRAGGPIRGGGEEG-----RGFRSR 284

Query: 277 SG-PYERRGSSGG 242
           +G PYER   SGG
Sbjct: 285 AGAPYERPSRSGG 297

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 828,348,622
Number of Sequences: 1393205
Number of extensions: 21094577
Number of successful extensions: 170876
Number of sequences better than 10.0: 6701
Number of HSP's better than 10.0 without gapping: 88832
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 130780
length of database: 448,689,247
effective HSP length: 123
effective length of database: 277,325,032
effective search space used: 49363855696
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GENLf066c02 BP065903 1 506 2 MF097c07_f BP033342 421 906

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