Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC006287A_C01 KMC006287A_c01
(1052 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAA78744.1| EST AU065533(C2174) corresponds to a region of t... 366 e-169
ref|NP_178124.1| splicing factor Prp8, putative; protein id: At1... 365 e-169
pir||B96832 hypothetical protein F18B13.15 [imported] - Arabidop... 365 e-169
ref|NP_195589.1| splicing factor - like protein; protein id: At4... 357 e-162
gb|AAC61776.1| splicing factor Prp8 [Homo sapiens] 315 e-142
>dbj|BAA78744.1| EST AU065533(C2174) corresponds to a region of the predicted
gene.~Similar to Homo sapiens splicing factor Prp8 mRNA,
complete cds.(AF092565) [Oryza sativa (japonica
cultivar-group)]
Length = 2391
Score = 366 bits (939), Expect(2) = e-169
Identities = 186/221 (84%), Positives = 193/221 (87%), Gaps = 1/221 (0%)
Frame = +2
Query: 392 FCFTHRRGSWTLHSAISH*CTLYPN-NREHVWGTII*KCWPRDARMRLMKHDVNLGRSVF 568
F FTH I T +P+ N E++ G KCWPRDARMRLMKHDVNLGRSVF
Sbjct: 1124 FRFTHEEA----RDLIQRYLTEHPDPNNENMVGYNNKKCWPRDARMRLMKHDVNLGRSVF 1179
Query: 569 WDMKNRLPRSITTLEWENSFVSVYSKDNPNLLFSMCGFEVRILPKIRMTQEAFSNTRDGV 748
WDMKNRLPRSITTLEWENSFVSVYSKDNPNLLFSMCGFEVRILPKIRMTQEAFSNT+DGV
Sbjct: 1180 WDMKNRLPRSITTLEWENSFVSVYSKDNPNLLFSMCGFEVRILPKIRMTQEAFSNTKDGV 1239
Query: 749 WNLQNEQTKERTAVAFLRVDDEHMKVFENRVRQILMSSGSTTFTKIVNKWNTALIGLMTY 928
WNLQNEQTKERTA+AFLRVDDEHMKVFENRVRQILMSSGSTTFTKIVNKWNTALIGLMTY
Sbjct: 1240 WNLQNEQTKERTAIAFLRVDDEHMKVFENRVRQILMSSGSTTFTKIVNKWNTALIGLMTY 1299
Query: 929 FREATVHTQELLDLLVKCENKIQTRIKIGLTQRCLRRFPPV 1051
FREATVHTQELLDLLVKCENKIQTRIKIGL + RFPPV
Sbjct: 1300 FREATVHTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPV 1340
Score = 254 bits (648), Expect(2) = e-169
Identities = 125/131 (95%), Positives = 129/131 (98%)
Frame = +1
Query: 1 SDGQCVVMLQTKFEKFFEKIDLTMLNRLLRLVLDHNIADYVTAKNNVVLSYKDMSHTNSY 180
SDGQCVVMLQTKFEKFFEKIDLT+LNRLLRLVLDHNIADYVTAKNNVVLSYKDMSHTNSY
Sbjct: 994 SDGQCVVMLQTKFEKFFEKIDLTLLNRLLRLVLDHNIADYVTAKNNVVLSYKDMSHTNSY 1053
Query: 181 GLIRGLQFASFVVQYYGLVLDLLLLGLTRASEIAGPPQMPNEFITYWDTKVETRHPIRLY 360
GLIRGLQFASFVVQYYGLVLDLLLLGLTRASEIAGPP MPNEF+TY DTKVETRHPIRLY
Sbjct: 1054 GLIRGLQFASFVVQYYGLVLDLLLLGLTRASEIAGPPTMPNEFLTYADTKVETRHPIRLY 1113
Query: 361 SRYIDRVHILF 393
SRYID+VHI+F
Sbjct: 1114 SRYIDKVHIMF 1124
>ref|NP_178124.1| splicing factor Prp8, putative; protein id: At1g80070.1 [Arabidopsis
thaliana]
Length = 2382
Score = 365 bits (936), Expect(2) = e-169
Identities = 186/221 (84%), Positives = 192/221 (86%), Gaps = 1/221 (0%)
Frame = +2
Query: 392 FCFTHRRGSWTLHSAISH*CTLYPN-NREHVWGTII*KCWPRDARMRLMKHDVNLGRSVF 568
F FTH I T +P+ N E++ G KCWPRDARMRLMKHDVNLGRSVF
Sbjct: 1145 FKFTHEEA----RDLIQRYLTEHPDPNNENMVGYNNKKCWPRDARMRLMKHDVNLGRSVF 1200
Query: 569 WDMKNRLPRSITTLEWENSFVSVYSKDNPNLLFSMCGFEVRILPKIRMTQEAFSNTRDGV 748
WDMKNRLPRSITTLEWEN FVSVYSKDNPNLLFSMCGFEVRILPKIRMTQEAFSNT+DGV
Sbjct: 1201 WDMKNRLPRSITTLEWENGFVSVYSKDNPNLLFSMCGFEVRILPKIRMTQEAFSNTKDGV 1260
Query: 749 WNLQNEQTKERTAVAFLRVDDEHMKVFENRVRQILMSSGSTTFTKIVNKWNTALIGLMTY 928
WNLQNEQTKERTAVAFLRVDDEHMKVFENRVRQILMSSGSTTFTKIVNKWNTALIGLMTY
Sbjct: 1261 WNLQNEQTKERTAVAFLRVDDEHMKVFENRVRQILMSSGSTTFTKIVNKWNTALIGLMTY 1320
Query: 929 FREATVHTQELLDLLVKCENKIQTRIKIGLTQRCLRRFPPV 1051
FREATVHTQELLDLLVKCENKIQTRIKIGL + RFPPV
Sbjct: 1321 FREATVHTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPV 1361
Score = 255 bits (651), Expect(2) = e-169
Identities = 123/130 (94%), Positives = 130/130 (99%)
Frame = +1
Query: 4 DGQCVVMLQTKFEKFFEKIDLTMLNRLLRLVLDHNIADYVTAKNNVVLSYKDMSHTNSYG 183
DGQCVVMLQTKFEKFFEKIDLTMLNRLLRLVLDHNIADYV+AKNNVVLSYKDMSHTNSYG
Sbjct: 1016 DGQCVVMLQTKFEKFFEKIDLTMLNRLLRLVLDHNIADYVSAKNNVVLSYKDMSHTNSYG 1075
Query: 184 LIRGLQFASFVVQYYGLVLDLLLLGLTRASEIAGPPQMPNEFITYWDTKVETRHPIRLYS 363
LIRGLQFASFVVQ+YGL+LDLLLLGLTRASEIAGPPQMPNEF+T+WDTKVETRHPIRLYS
Sbjct: 1076 LIRGLQFASFVVQFYGLLLDLLLLGLTRASEIAGPPQMPNEFMTFWDTKVETRHPIRLYS 1135
Query: 364 RYIDRVHILF 393
RYID+VHI+F
Sbjct: 1136 RYIDKVHIMF 1145
>pir||B96832 hypothetical protein F18B13.15 [imported] - Arabidopsis thaliana
gi|5902365|gb|AAD55467.1|AC009322_7 Putative splicing
factor Prp8 [Arabidopsis thaliana]
Length = 2359
Score = 365 bits (936), Expect(2) = e-169
Identities = 186/221 (84%), Positives = 192/221 (86%), Gaps = 1/221 (0%)
Frame = +2
Query: 392 FCFTHRRGSWTLHSAISH*CTLYPN-NREHVWGTII*KCWPRDARMRLMKHDVNLGRSVF 568
F FTH I T +P+ N E++ G KCWPRDARMRLMKHDVNLGRSVF
Sbjct: 1122 FKFTHEEA----RDLIQRYLTEHPDPNNENMVGYNNKKCWPRDARMRLMKHDVNLGRSVF 1177
Query: 569 WDMKNRLPRSITTLEWENSFVSVYSKDNPNLLFSMCGFEVRILPKIRMTQEAFSNTRDGV 748
WDMKNRLPRSITTLEWEN FVSVYSKDNPNLLFSMCGFEVRILPKIRMTQEAFSNT+DGV
Sbjct: 1178 WDMKNRLPRSITTLEWENGFVSVYSKDNPNLLFSMCGFEVRILPKIRMTQEAFSNTKDGV 1237
Query: 749 WNLQNEQTKERTAVAFLRVDDEHMKVFENRVRQILMSSGSTTFTKIVNKWNTALIGLMTY 928
WNLQNEQTKERTAVAFLRVDDEHMKVFENRVRQILMSSGSTTFTKIVNKWNTALIGLMTY
Sbjct: 1238 WNLQNEQTKERTAVAFLRVDDEHMKVFENRVRQILMSSGSTTFTKIVNKWNTALIGLMTY 1297
Query: 929 FREATVHTQELLDLLVKCENKIQTRIKIGLTQRCLRRFPPV 1051
FREATVHTQELLDLLVKCENKIQTRIKIGL + RFPPV
Sbjct: 1298 FREATVHTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPV 1338
Score = 255 bits (651), Expect(2) = e-169
Identities = 123/130 (94%), Positives = 130/130 (99%)
Frame = +1
Query: 4 DGQCVVMLQTKFEKFFEKIDLTMLNRLLRLVLDHNIADYVTAKNNVVLSYKDMSHTNSYG 183
DGQCVVMLQTKFEKFFEKIDLTMLNRLLRLVLDHNIADYV+AKNNVVLSYKDMSHTNSYG
Sbjct: 993 DGQCVVMLQTKFEKFFEKIDLTMLNRLLRLVLDHNIADYVSAKNNVVLSYKDMSHTNSYG 1052
Query: 184 LIRGLQFASFVVQYYGLVLDLLLLGLTRASEIAGPPQMPNEFITYWDTKVETRHPIRLYS 363
LIRGLQFASFVVQ+YGL+LDLLLLGLTRASEIAGPPQMPNEF+T+WDTKVETRHPIRLYS
Sbjct: 1053 LIRGLQFASFVVQFYGLLLDLLLLGLTRASEIAGPPQMPNEFMTFWDTKVETRHPIRLYS 1112
Query: 364 RYIDRVHILF 393
RYID+VHI+F
Sbjct: 1113 RYIDKVHIMF 1122
>ref|NP_195589.1| splicing factor - like protein; protein id: At4g38780.1 [Arabidopsis
thaliana] gi|7488347|pir||T06077 splicing factor PRP8
homolog T9A14.60 - Arabidopsis thaliana
gi|4490330|emb|CAB38612.1| splicing factor-like protein
[Arabidopsis thaliana] gi|7270861|emb|CAB80541.1|
splicing factor-like protein [Arabidopsis thaliana]
Length = 2352
Score = 357 bits (917), Expect(2) = e-162
Identities = 177/197 (89%), Positives = 183/197 (92%)
Frame = +2
Query: 461 PNNREHVWGTII*KCWPRDARMRLMKHDVNLGRSVFWDMKNRLPRSITTLEWENSFVSVY 640
PNN E++ G KCWPRDARMRLMKHDVNLGRSVFWDMKNRLPRSITTLEWEN FVSVY
Sbjct: 1138 PNN-ENMVGYNNKKCWPRDARMRLMKHDVNLGRSVFWDMKNRLPRSITTLEWENGFVSVY 1196
Query: 641 SKDNPNLLFSMCGFEVRILPKIRMTQEAFSNTRDGVWNLQNEQTKERTAVAFLRVDDEHM 820
SKDNPNLLFSMCGFEVR+LPKIRM QEAFS+TRDGVWNLQNEQTKERTAVAFLR DDEHM
Sbjct: 1197 SKDNPNLLFSMCGFEVRVLPKIRMGQEAFSSTRDGVWNLQNEQTKERTAVAFLRADDEHM 1256
Query: 821 KVFENRVRQILMSSGSTTFTKIVNKWNTALIGLMTYFREATVHTQELLDLLVKCENKIQT 1000
KVFENRVRQILMSSGSTTFTKIVNKWNTALIGLMTYFREATVHTQELLDLLVKCENKIQT
Sbjct: 1257 KVFENRVRQILMSSGSTTFTKIVNKWNTALIGLMTYFREATVHTQELLDLLVKCENKIQT 1316
Query: 1001 RIKIGLTQRCLRRFPPV 1051
R+KIGL + RFPPV
Sbjct: 1317 RVKIGLNSKMPSRFPPV 1333
Score = 238 bits (608), Expect(2) = e-162
Identities = 116/131 (88%), Positives = 125/131 (94%)
Frame = +1
Query: 1 SDGQCVVMLQTKFEKFFEKIDLTMLNRLLRLVLDHNIADYVTAKNNVVLSYKDMSHTNSY 180
SDGQCVVMLQTKFEK FEKIDLT+LN LLRLVLD +A+YVT KNNVVLSYKDMS+TN+Y
Sbjct: 987 SDGQCVVMLQTKFEKLFEKIDLTVLNSLLRLVLDPKLANYVTGKNNVVLSYKDMSYTNTY 1046
Query: 181 GLIRGLQFASFVVQYYGLVLDLLLLGLTRASEIAGPPQMPNEFITYWDTKVETRHPIRLY 360
GLIRGLQFASFVVQ+YGLVLDLLLLGLTRASEIAGPPQ PNEF+TYWDTKVETRHPIRLY
Sbjct: 1047 GLIRGLQFASFVVQFYGLVLDLLLLGLTRASEIAGPPQRPNEFMTYWDTKVETRHPIRLY 1106
Query: 361 SRYIDRVHILF 393
SRYID+VHI+F
Sbjct: 1107 SRYIDKVHIMF 1117
>gb|AAC61776.1| splicing factor Prp8 [Homo sapiens]
Length = 2335
Score = 315 bits (808), Expect(2) = e-142
Identities = 157/197 (79%), Positives = 173/197 (87%)
Frame = +2
Query: 461 PNNREHVWGTII*KCWPRDARMRLMKHDVNLGRSVFWDMKNRLPRSITTLEWENSFVSVY 640
PNN E++ G KCWPRDARMRLMKHDVNLGR+VFWD+KNRLPRS+TT++WENSFVSVY
Sbjct: 1120 PNN-ENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVY 1178
Query: 641 SKDNPNLLFSMCGFEVRILPKIRMTQEAFSNTRDGVWNLQNEQTKERTAVAFLRVDDEHM 820
SKDNPNLLF+MCGFE RILPK R + E F++ +DGVWNLQNE TKERTA FLRVDDE M
Sbjct: 1179 SKDNPNLLFNMCGFECRILPKCRTSYEEFTH-KDGVWNLQNEVTKERTAQCFLRVDDESM 1237
Query: 821 KVFENRVRQILMSSGSTTFTKIVNKWNTALIGLMTYFREATVHTQELLDLLVKCENKIQT 1000
+ F NRVRQILM+SGSTTFTKIVNKWNTALIGLMTYFREA V+TQELLDLLVKCENKIQT
Sbjct: 1238 QRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQT 1297
Query: 1001 RIKIGLTQRCLRRFPPV 1051
RIKIGL + RFPPV
Sbjct: 1298 RIKIGLNSKMPSRFPPV 1314
Score = 213 bits (542), Expect(2) = e-142
Identities = 96/131 (73%), Positives = 120/131 (91%)
Frame = +1
Query: 1 SDGQCVVMLQTKFEKFFEKIDLTMLNRLLRLVLDHNIADYVTAKNNVVLSYKDMSHTNSY 180
S+G+C VML+++FEK +EKIDLT+LNRLLRL++DHNIADY+TAKNNVV++YKDM+HTNSY
Sbjct: 969 SEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSY 1028
Query: 181 GLIRGLQFASFVVQYYGLVLDLLLLGLTRASEIAGPPQMPNEFITYWDTKVETRHPIRLY 360
G+IRGLQFASF+VQYYGLV+DLL+LGL RASE+AGPPQMPN+F+++ D E HPIRL+
Sbjct: 1029 GIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLF 1088
Query: 361 SRYIDRVHILF 393
RYIDR+HI F
Sbjct: 1089 CRYIDRIHIFF 1099
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 882,371,331
Number of Sequences: 1393205
Number of extensions: 19144855
Number of successful extensions: 46262
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 44390
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 46231
length of database: 448,689,247
effective HSP length: 124
effective length of database: 275,931,827
effective search space used: 62360592902
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
