>KMC005856A_C02 KMC005856A_c02
tttttttttttttgGCCAAAACAAGTAAGGAGACTGAATATACATGAGTGTTAGTAATAA
TATTGGTAACACCTACAAAATATACATGGTGATTTGAGGGACAAACGTTTCCCCAAAATT
GGCACCCAAGTCCTTTTATTGATATCCAAAAACCTGCTTGTCTCATCAGTCATCACTGGA
ATGATGGCAACTAAAAACTGGTAACATTCACAAGGGAACAAGATGGTTATTCGTCTGCAT
CTTCTACTTCGTCTTGAAATACTTCATTTTCTGCAGCAGGAACCAATTCATCAGGAATTT
TCGCCACATCTGCATTTGCTGCAGCCAATGTCTCCATTGTCCAATTGAAATTGCTAACCT
CGCGTTCTTTTGAAGCTGAGGATCCATCCGGAATGTTTGCAACAATATCTGCTGCAGTAG
AAGTCTCTTGTACCCCGACCAAATTGCTAATACTGTTTTCTTCTTGGCTTTCATCCACTC
TATAATATACCACACAGGTACGAGGAGGGGAGAGGATCTGGAAGGAATngggccga

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC005856A_C02 KMC005856A_c02
         (536 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

pir||T06494 1,4-alpha-glucan branching enzyme (EC 2.4.1.18) II -...    74  1e-12
dbj|BAA82349.1| branching enzyme 3 [Phaseolus vulgaris]                70  2e-11
pir||T07824 1,4-alpha-glucan branching enzyme (EC 2.4.1.18) I (c...    53  3e-06
emb|CAA54308.1| 1,4-alpha-glucan branching enzyme [Manihot escul...    50  2e-05
gb|AAD50279.2| seed starch branching enzyme [Sorghum bicolor]          49  3e-05

>pir||T06494 1,4-alpha-glucan branching enzyme (EC 2.4.1.18) II - garden pea
           (fragment) gi|1345571|emb|CAA56320.1| starch branching
           enzyme II [Pisum sativum]
          Length = 826

 Score = 73.6 bits (179), Expect = 1e-12
 Identities = 42/86 (48%), Positives = 54/86 (61%), Gaps = 1/86 (1%)
 Frame = -2

Query: 535 RPXSFQILSPPRTCVVYYRVDESQEENSISNLVGVQETSTAADI-VANIPDGSSASKERE 359
           RP SF++LSPP TCVVYYRVDE QEE++  NL  V+ET  AAD  VA IPD S  S++  
Sbjct: 740 RPNSFKVLSPPHTCVVYYRVDERQEESNNPNLGSVEETFAAADTDVARIPDVSMESEDSN 799

Query: 358 VSNFNWTMETLAAANADVAKIPDELV 281
           +       E   A +A + K+  E+V
Sbjct: 800 LDRIEDNSED--AVDAGILKVEREVV 823

>dbj|BAA82349.1| branching enzyme 3 [Phaseolus vulgaris]
          Length = 847

 Score = 70.1 bits (170), Expect = 2e-11
 Identities = 39/77 (50%), Positives = 52/77 (66%)
 Frame = -2

Query: 535 RPXSFQILSPPRTCVVYYRVDESQEENSISNLVGVQETSTAADIVANIPDGSSASKEREV 356
           RP SF++LSP RTCVVYYRVDE+Q E S  +LVG+++T  AAD VA IPD S++ +    
Sbjct: 752 RPNSFKVLSPARTCVVYYRVDENQ-EGSNDSLVGLEDTFAAAD-VAKIPDKSASIESEYS 809

Query: 355 SNFNWTMETLAAANADV 305
           +N +   ET  +A   V
Sbjct: 810 NNLDGVKETSTSAQISV 826

>pir||T07824 1,4-alpha-glucan branching enzyme (EC 2.4.1.18) I (clone sbeI7) -
           potato (fragment) gi|1621012|emb|CAA70038.1|
           1,4-alpha-glucan branching enzyme [Solanum tuberosum]
          Length = 830

 Score = 52.8 bits (125), Expect = 3e-06
 Identities = 32/102 (31%), Positives = 51/102 (49%), Gaps = 2/102 (1%)
 Frame = -2

Query: 535 RPXSFQILSPPRTCVVYYRVDESQEENSISNLVGVQETSTAADIV--ANIPDGSSASKER 362
           RP SF++LSP RTCV YYRVDE   E          +T   ++++  ANI +     K+ 
Sbjct: 684 RPNSFKVLSPARTCVAYYRVDERMSETE------DYQTDICSELLPTANIEESDEKLKDS 737

Query: 361 EVSNFNWTMETLAAANADVAKIPDELVPAAENEVFQDEVEDA 236
             +N +   E ++        I  EL+P A  E   ++++D+
Sbjct: 738 LSTNISNIDERMSETEVYQTDISSELLPTANIEESDEKLKDS 779

>emb|CAA54308.1| 1,4-alpha-glucan branching enzyme [Manihot esculenta]
          Length = 852

 Score = 50.1 bits (118), Expect = 2e-05
 Identities = 34/102 (33%), Positives = 51/102 (49%)
 Frame = -2

Query: 535  RPXSFQILSPPRTCVVYYRVDESQEENSISNLVGVQETSTAADIVANIPDGSSASKEREV 356
            RP SF+ILS  RTCVVYYRV+E +  ++ S++    ET T    + +    +  S    V
Sbjct: 756  RPNSFKILSAARTCVVYYRVEEKEGNHNSSDIGAANETLTDIAKLGDFEGINETSPADAV 815

Query: 355  SNFNWTMETLAAANADVAKIPDELVPAAENEVFQDEVEDADE 230
            +      E L AA   +  I D++   A  E  + E E +D+
Sbjct: 816  AK----QEDLKAAQPSL--IADDIATKANTETEEIEEETSDD 851

>gb|AAD50279.2| seed starch branching enzyme [Sorghum bicolor]
          Length = 832

 Score = 49.3 bits (116), Expect = 3e-05
 Identities = 28/59 (47%), Positives = 37/59 (62%), Gaps = 2/59 (3%)
 Frame = -2

Query: 535 RPXSFQILSPPRTCVVYYRVDESQEE-NSISNLVGVQETS-TAADIVANIPDGSSASKE 365
           RP SF++LSPPRTCV YYRVDE  E   + +   G ++TS    D+ A     S+A+KE
Sbjct: 745 RPNSFKVLSPPRTCVAYYRVDEEAERLQAKAETTGSRKTSPDIIDVDATPVKTSTATKE 803

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 482,722,404
Number of Sequences: 1393205
Number of extensions: 10535207
Number of successful extensions: 29938
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 28607
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 29802
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 18173652336
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 SPD008e09_f BP044645 1 536 2 GENLf007g02 BP062730 16 515

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