Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC005771A_C01 KMC005771A_c01
(595 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAB19212.1| polygalacturonase-inhibiting protein [Malus x dom... 127 3e-44
pir||S47965 polygalacturonase inhibitor protein - tomato gi|4694... 130 4e-44
gb|AAB80732.1| polygalacturonase inhibiting protein [Prunus arme... 124 2e-42
gb|AAF79181.1| polygalacturonase inhibiting protein [Prunus maha... 123 2e-42
sp|Q05091|PGIP_PYRCO Polygalacturonase inhibitor precursor (Poly... 127 2e-42
>gb|AAB19212.1| polygalacturonase-inhibiting protein [Malus x domestica]
Length = 330
Score = 127 bits (320), Expect(2) = 3e-44
Identities = 64/105 (60%), Positives = 79/105 (74%)
Frame = -3
Query: 404 EGDASFLFGPNKKIRILDLSKNLLSFDFSNVEIPTKSLIWLDLNHNMIHGSIPIGLTAVE 225
EGDAS +FG NK +I+DLS+NLL F+ S VE PT SL LD+NHN I+GSIP+ T +
Sbjct: 228 EGDASVIFGLNKTTQIVDLSRNLLEFNLSKVEFPT-SLTSLDINHNKIYGSIPVEFTQL- 285
Query: 224 NLQQFNVSYNQLSGKIPKGGELGRFDKYSYLHNKDLCGSPLPMCK 90
N Q NVSYN+L G+IP GG+L FD+YSY HN+ LCG+PLP CK
Sbjct: 286 NFQFLNVSYNRLCGQIPVGGKLQSFDEYSYFHNRCLCGAPLPSCK 330
Score = 72.8 bits (177), Expect(2) = 3e-44
Identities = 38/64 (59%), Positives = 45/64 (69%)
Frame = -1
Query: 595 LSK*LNLSSLHLDQNQLTGPIPSSLGSLQKPGPDIVLSHNQLSGPIPASLGQLDPDRIDF 416
LS+ NL++LHLD+N+LTG IP SLG PD+ LSHNQLSG IP S Q+D ID
Sbjct: 163 LSQLPNLNALHLDRNKLTGHIPKSLGQFIGNVPDLYLSHNQLSGNIPTSFAQMDFTSIDL 222
Query: 415 SRNK 404
SRNK
Sbjct: 223 SRNK 226
Score = 44.7 bits (104), Expect = 0.001
Identities = 33/107 (30%), Positives = 51/107 (46%)
Frame = -3
Query: 470 FRAHSCLLGPVRPGQDRLFKEQEGDASFLFGPNKKIRILDLSKNLLSFDFSNVEIPTKSL 291
F L GP++P +L K ++ L LS LS + K+L
Sbjct: 101 FHKQPNLTGPIQPAIAKL---------------KGLKFLRLSWTNLSGSVPDFLSQLKNL 145
Query: 290 IWLDLNHNMIHGSIPIGLTAVENLQQFNVSYNQLSGKIPKGGELGRF 150
+LDL+ N + G+IP L+ + NL ++ N+L+G IPK LG+F
Sbjct: 146 TFLDLSFNNLTGAIPSSLSQLPNLNALHLDRNKLTGHIPK--SLGQF 190
>pir||S47965 polygalacturonase inhibitor protein - tomato
gi|469457|gb|AAA53547.1| polygalacturonase inhibitor
protein
Length = 327
Score = 130 bits (328), Expect(2) = 4e-44
Identities = 69/105 (65%), Positives = 78/105 (73%)
Frame = -3
Query: 404 EGDASFLFGPNKKIRILDLSKNLLSFDFSNVEIPTKSLIWLDLNHNMIHGSIPIGLTAVE 225
EGD SFLFG NK +++DLS+NLL FD S E +SLI LDLNHN I GS+P GL V
Sbjct: 225 EGDVSFLFGKNKTSQVIDLSRNLLEFDISKSEF-AESLISLDLNHNRIFGSLPPGLKDVP 283
Query: 224 NLQQFNVSYNQLSGKIPKGGELGRFDKYSYLHNKDLCGSPLPMCK 90
LQ FNVSYN+L G+IP+GG L FD YSYLHNK LCGSPLP CK
Sbjct: 284 -LQFFNVSYNRLCGQIPQGGTLQSFDIYSYLHNKCLCGSPLPKCK 327
Score = 69.3 bits (168), Expect(2) = 4e-44
Identities = 35/65 (53%), Positives = 47/65 (71%), Gaps = 1/65 (1%)
Frame = -1
Query: 595 LSK*LNLSSLHLDQNQLTGPIPSSLGSLQKPG-PDIVLSHNQLSGPIPASLGQLDPDRID 419
LS+ NL +++LD+N+LTG IP S G + P PD+ LSHN L+G +PASLG L+ +D
Sbjct: 159 LSQLPNLLAMYLDRNKLTGTIPESFGRFKGPNIPDLYLSHNSLTGHVPASLGDLNFSTLD 218
Query: 418 FSRNK 404
FSRNK
Sbjct: 219 FSRNK 223
Score = 38.5 bits (88), Expect = 0.065
Identities = 24/70 (34%), Positives = 37/70 (52%), Gaps = 7/70 (10%)
Frame = -3
Query: 338 LLSFDFSNVEIPT-------KSLIWLDLNHNMIHGSIPIGLTAVENLQQFNVSYNQLSGK 180
+L F+N+ P K+L L+LN+N G+IP L+ + NL + N+L+G
Sbjct: 119 MLRLSFTNLTGPIPEFLSQLKNLTLLELNYNQFTGTIPSSLSQLPNLLAMYLDRNKLTGT 178
Query: 179 IPKGGELGRF 150
IP+ GRF
Sbjct: 179 IPE--SFGRF 186
>gb|AAB80732.1| polygalacturonase inhibiting protein [Prunus armeniaca]
Length = 330
Score = 124 bits (311), Expect(2) = 2e-42
Identities = 64/105 (60%), Positives = 78/105 (73%)
Frame = -3
Query: 404 EGDASFLFGPNKKIRILDLSKNLLSFDFSNVEIPTKSLIWLDLNHNMIHGSIPIGLTAVE 225
EGDAS +FG NK +I+DLS+NLL + SNVE +KSL LDLNHN I G IP+GLT V+
Sbjct: 228 EGDASMIFGLNKTTQIVDLSRNLLEINLSNVEF-SKSLTSLDLNHNKITGGIPVGLTQVD 286
Query: 224 NLQQFNVSYNQLSGKIPKGGELGRFDKYSYLHNKDLCGSPLPMCK 90
LQ NVSYN+L G+IP GG+L FD +Y HN+ LCG+PLP CK
Sbjct: 287 -LQFLNVSYNRLCGQIPVGGKLQSFDSSTYFHNRCLCGAPLPSCK 330
Score = 70.5 bits (171), Expect(2) = 2e-42
Identities = 36/64 (56%), Positives = 45/64 (70%)
Frame = -1
Query: 595 LSK*LNLSSLHLDQNQLTGPIPSSLGSLQKPGPDIVLSHNQLSGPIPASLGQLDPDRIDF 416
LS+ NL++L +D+N+LTG IP S G PD+ LSHNQLSG IP SL +L+ IDF
Sbjct: 163 LSQLPNLNALRVDRNKLTGHIPKSFGEFDGSVPDLYLSHNQLSGTIPTSLAKLNFSTIDF 222
Query: 415 SRNK 404
SRNK
Sbjct: 223 SRNK 226
Score = 42.7 bits (99), Expect = 0.003
Identities = 36/122 (29%), Positives = 54/122 (43%), Gaps = 1/122 (0%)
Frame = -3
Query: 470 FRAHSCLLGPVRPGQDRLFKEQEGDASFLFGPNKKIRILDLSKNLLSFDFSNVEIPTKSL 291
F L GP++P +L K ++ L LS +S + K+L
Sbjct: 101 FHKQPNLTGPIQPSIAKL---------------KLLKELRLSWTNISGSVPDFLSQLKNL 145
Query: 290 IWLDLNHNMIHGSIPIGLTAVENLQQFNVSYNQLSGKIPKG-GELGRFDKYSYLHNKDLC 114
+LDL+ + + GSIP L+ + NL V N+L+G IPK GE YL + L
Sbjct: 146 TFLDLSFSNLTGSIPSWLSQLPNLNALRVDRNKLTGHIPKSFGEFDGSVPDLYLSHNQLS 205
Query: 113 GS 108
G+
Sbjct: 206 GT 207
>gb|AAF79181.1| polygalacturonase inhibiting protein [Prunus mahaleb]
Length = 330
Score = 123 bits (308), Expect(2) = 2e-42
Identities = 63/105 (60%), Positives = 78/105 (74%)
Frame = -3
Query: 404 EGDASFLFGPNKKIRILDLSKNLLSFDFSNVEIPTKSLIWLDLNHNMIHGSIPIGLTAVE 225
EGDAS +FG NK +I+DLS+NLL F+ S VE +KSL LDLNHN I G IP+GLT ++
Sbjct: 228 EGDASMIFGLNKTTQIVDLSRNLLEFNLSKVEF-SKSLTSLDLNHNKITGGIPVGLTQLD 286
Query: 224 NLQQFNVSYNQLSGKIPKGGELGRFDKYSYLHNKDLCGSPLPMCK 90
LQ NVSYN+L G+IP GG+L FD +Y HN+ LCG+PLP CK
Sbjct: 287 -LQFLNVSYNRLCGQIPVGGKLQSFDSSTYFHNRCLCGAPLPSCK 330
Score = 71.6 bits (174), Expect(2) = 2e-42
Identities = 37/64 (57%), Positives = 45/64 (69%)
Frame = -1
Query: 595 LSK*LNLSSLHLDQNQLTGPIPSSLGSLQKPGPDIVLSHNQLSGPIPASLGQLDPDRIDF 416
LS+ NL++L LD+N+LTG IP S G PD+ LSHNQLSG IP SL +L+ IDF
Sbjct: 163 LSQLPNLNALRLDRNKLTGHIPKSFGEFHGSVPDLYLSHNQLSGTIPTSLAKLNFSTIDF 222
Query: 415 SRNK 404
SRNK
Sbjct: 223 SRNK 226
Score = 42.7 bits (99), Expect = 0.003
Identities = 35/122 (28%), Positives = 54/122 (43%), Gaps = 1/122 (0%)
Frame = -3
Query: 470 FRAHSCLLGPVRPGQDRLFKEQEGDASFLFGPNKKIRILDLSKNLLSFDFSNVEIPTKSL 291
F L GP++P +L K ++ L LS +S + K+L
Sbjct: 101 FHKQPNLTGPIQPSIAKL---------------KSLKELRLSWTNISGSVPDFLSQLKNL 145
Query: 290 IWLDLNHNMIHGSIPIGLTAVENLQQFNVSYNQLSGKIPKG-GELGRFDKYSYLHNKDLC 114
+LDL+ + + GSIP L+ + NL + N+L+G IPK GE YL + L
Sbjct: 146 TFLDLSFSNLTGSIPSSLSQLPNLNALRLDRNKLTGHIPKSFGEFHGSVPDLYLSHNQLS 205
Query: 113 GS 108
G+
Sbjct: 206 GT 207
>sp|Q05091|PGIP_PYRCO Polygalacturonase inhibitor precursor (Polygalacturonase-inhibiting
protein) gi|543660|pir||JQ2262 Polygalacturonase
inhibitor precursor - Pyrus communis
gi|169684|gb|AAA33865.1| polygalacturonase inhibitor
Length = 330
Score = 127 bits (320), Expect(2) = 2e-42
Identities = 64/105 (60%), Positives = 79/105 (74%)
Frame = -3
Query: 404 EGDASFLFGPNKKIRILDLSKNLLSFDFSNVEIPTKSLIWLDLNHNMIHGSIPIGLTAVE 225
EGDAS +FG NK +I+DLS+NLL F+ S VE PT SL LD+NHN I+GSIP+ T +
Sbjct: 228 EGDASVIFGLNKTTQIVDLSRNLLEFNLSKVEFPT-SLTSLDINHNKIYGSIPVEFTQL- 285
Query: 224 NLQQFNVSYNQLSGKIPKGGELGRFDKYSYLHNKDLCGSPLPMCK 90
N Q NVSYN+L G+IP GG+L FD+YSY HN+ LCG+PLP CK
Sbjct: 286 NFQFLNVSYNRLCGQIPVGGKLQSFDEYSYFHNRCLCGAPLPSCK 330
Score = 67.0 bits (162), Expect(2) = 2e-42
Identities = 36/64 (56%), Positives = 42/64 (65%)
Frame = -1
Query: 595 LSK*LNLSSLHLDQNQLTGPIPSSLGSLQKPGPDIVLSHNQLSGPIPASLGQLDPDRIDF 416
LS+ NL +L LD+N+LTG IP S G PD+ LSHNQLSG IP S Q+D ID
Sbjct: 163 LSELPNLGALRLDRNKLTGHIPISFGQFIGNVPDLYLSHNQLSGNIPTSFAQMDFTSIDL 222
Query: 415 SRNK 404
SRNK
Sbjct: 223 SRNK 226
Score = 39.3 bits (90), Expect = 0.038
Identities = 29/99 (29%), Positives = 45/99 (45%)
Frame = -3
Query: 470 FRAHSCLLGPVRPGQDRLFKEQEGDASFLFGPNKKIRILDLSKNLLSFDFSNVEIPTKSL 291
F L GP++P +L K ++ L LS LS + K+L
Sbjct: 101 FHKQPNLTGPIQPAIAKL---------------KGLKSLRLSWTNLSGSVPDFLSQLKNL 145
Query: 290 IWLDLNHNMIHGSIPIGLTAVENLQQFNVSYNQLSGKIP 174
+LDL+ N + G+IP L+ + NL + N+L+G IP
Sbjct: 146 TFLDLSFNNLTGAIPSSLSELPNLGALRLDRNKLTGHIP 184
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 528,172,207
Number of Sequences: 1393205
Number of extensions: 11894433
Number of successful extensions: 51976
Number of sequences better than 10.0: 1015
Number of HSP's better than 10.0 without gapping: 41904
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 49597
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 22854740960
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
