Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC005762A_C01 KMC005762A_c01
(655 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_192425.1| 4-coumarate--CoA ligase - like protein; protein... 236 2e-61
ref|NP_173474.1| hypothetical protein; protein id: At1g20500.1 [... 195 5e-49
ref|NP_564115.1| expressed protein; protein id: At1g20510.1 [Ara... 194 1e-48
dbj|BAB89960.1| putative 4-coumarate-CoA ligase [Oryza sativa (j... 191 1e-47
gb|AAF91310.1|AF239687_1 4-coumarate:coA ligase 1 [Rubus idaeus] 188 5e-47
>ref|NP_192425.1| 4-coumarate--CoA ligase - like protein; protein id: At4g05160.1,
supported by cDNA: gi_20258833 [Arabidopsis thaliana]
gi|25293418|pir||H85064 4-coumarate-CoA ligase-like
protein [imported] - Arabidopsis thaliana
gi|7267275|emb|CAB81058.1| 4-coumarate--CoA ligase-like
protein [Arabidopsis thaliana]
gi|20258834|gb|AAM13899.1| putative 4-coumarate--CoA
ligase [Arabidopsis thaliana] gi|21689723|gb|AAM67483.1|
putative 4-coumarate--CoA ligase [Arabidopsis thaliana]
Length = 544
Score = 236 bits (602), Expect = 2e-61
Identities = 111/143 (77%), Positives = 133/143 (92%)
Frame = -1
Query: 640 GYHNNPEATKLTLDKKGWVHTGDVGYFDEDGQLYVVDRIKELIKYKGFQVAPAELEGLLV 461
GY NNP+ATK T+DKK WVHTGD+GYF+EDG LYVVDRIKELIKYKGFQVAPAELEGLLV
Sbjct: 402 GYLNNPQATKETIDKKSWVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLV 461
Query: 460 SHPEILDAAVVPYPDDEAGEVPIAYVVRSPNSSLTEKQIQEFIASQVAPFKKLRRVTFIS 281
SHP+ILDA V+P+PD+EAGEVPIA+VVRSPNSS+TE+ IQ+FIA QVAP+K+LRRV+FIS
Sbjct: 462 SHPDILDAVVIPFPDEEAGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFIS 521
Query: 280 SVPKTASGKLLRRELIAKAKSKI 212
VPK+A+GK+LRREL+ + +SK+
Sbjct: 522 LVPKSAAGKILRRELVQQVRSKM 544
>ref|NP_173474.1| hypothetical protein; protein id: At1g20500.1 [Arabidopsis
thaliana]
Length = 550
Score = 195 bits (495), Expect = 5e-49
Identities = 91/143 (63%), Positives = 120/143 (83%)
Frame = -1
Query: 640 GYHNNPEATKLTLDKKGWVHTGDVGYFDEDGQLYVVDRIKELIKYKGFQVAPAELEGLLV 461
GY N EAT T++ +GW+ TGD+ Y DEDG L+VVDR+KELIKYKG+QV PAELE LL+
Sbjct: 408 GYFKNQEATNETINLEGWLKTGDLCYIDEDGFLFVVDRLKELIKYKGYQVPPAELEALLI 467
Query: 460 SHPEILDAAVVPYPDDEAGEVPIAYVVRSPNSSLTEKQIQEFIASQVAPFKKLRRVTFIS 281
+HP+ILDAAV+P+PD EAG+ P+AYVVR S+L+EKQ+ +FI+ QVAP+KK+R+V+FI+
Sbjct: 468 THPDILDAAVIPFPDKEAGQYPMAYVVRKHESNLSEKQVIDFISKQVAPYKKIRKVSFIN 527
Query: 280 SVPKTASGKLLRRELIAKAKSKI 212
S+PKTASGK LR++LI A SK+
Sbjct: 528 SIPKTASGKTLRKDLIKLATSKL 550
>ref|NP_564115.1| expressed protein; protein id: At1g20510.1 [Arabidopsis thaliana]
gi|13430676|gb|AAK25960.1|AF360250_1 unknown protein
[Arabidopsis thaliana] gi|14532846|gb|AAK64105.1|
unknown protein [Arabidopsis thaliana]
Length = 546
Score = 194 bits (492), Expect = 1e-48
Identities = 90/141 (63%), Positives = 115/141 (80%)
Frame = -1
Query: 640 GYHNNPEATKLTLDKKGWVHTGDVGYFDEDGQLYVVDRIKELIKYKGFQVAPAELEGLLV 461
GY +N EAT TLD +GW+ TGD+ Y DEDG ++VVDR+KELIKYKG+QVAPAELE LL+
Sbjct: 402 GYFSNEEATSSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLL 461
Query: 460 SHPEILDAAVVPYPDDEAGEVPIAYVVRSPNSSLTEKQIQEFIASQVAPFKKLRRVTFIS 281
+HPEI DAAV+P+PD E G+ P+AYVVR SSL+EK I EF+A QVAP+K++R+V F+S
Sbjct: 462 THPEITDAAVIPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVS 521
Query: 280 SVPKTASGKLLRRELIAKAKS 218
S+PK SGK+LR++LI A S
Sbjct: 522 SIPKNPSGKILRKDLIKIATS 542
>dbj|BAB89960.1| putative 4-coumarate-CoA ligase [Oryza sativa (japonica
cultivar-group)] gi|20161607|dbj|BAB90527.1| putative
4-coumarate-CoA ligase [Oryza sativa (japonica
cultivar-group)]
Length = 542
Score = 191 bits (484), Expect = 1e-47
Identities = 84/141 (59%), Positives = 119/141 (83%)
Frame = -1
Query: 640 GYHNNPEATKLTLDKKGWVHTGDVGYFDEDGQLYVVDRIKELIKYKGFQVAPAELEGLLV 461
GY + EAT T+D +GW+ TGD+ YF+EDG LY+VDR+KELIKYKG+QV PAELE +L
Sbjct: 396 GYVGDDEATAATVDSEGWLKTGDLCYFNEDGFLYIVDRLKELIKYKGYQVPPAELEHILQ 455
Query: 460 SHPEILDAAVVPYPDDEAGEVPIAYVVRSPNSSLTEKQIQEFIASQVAPFKKLRRVTFIS 281
SHP I DAAV+PYPD+EAGE+P+A++VR P S++T++Q+ +++A QVAP+KK+RRV F++
Sbjct: 456 SHPGIADAAVIPYPDEEAGELPMAFIVRQPGSNITKEQVMDYVAKQVAPYKKVRRVAFVT 515
Query: 280 SVPKTASGKLLRRELIAKAKS 218
++PK+ +GK+LRREL+ +A S
Sbjct: 516 AIPKSPAGKILRRELVQQALS 536
>gb|AAF91310.1|AF239687_1 4-coumarate:coA ligase 1 [Rubus idaeus]
Length = 543
Score = 188 bits (478), Expect = 5e-47
Identities = 86/138 (62%), Positives = 115/138 (83%)
Frame = -1
Query: 640 GYHNNPEATKLTLDKKGWVHTGDVGYFDEDGQLYVVDRIKELIKYKGFQVAPAELEGLLV 461
GY N+PEAT+ T+DK+GW+HTGD+G+ D+D +L++VDR+KELIKYKGFQVAPAELE LLV
Sbjct: 399 GYLNDPEATRTTIDKQGWLHTGDIGFIDDDEELFIVDRLKELIKYKGFQVAPAELEALLV 458
Query: 460 SHPEILDAAVVPYPDDEAGEVPIAYVVRSPNSSLTEKQIQEFIASQVAPFKKLRRVTFIS 281
+HP I DAAVVP DD AGEVP+A+VV S +TE +I++FI+ QV +K+++RV FI
Sbjct: 459 THPNISDAAVVPMKDDAAGEVPVAFVVSPKGSQITEDEIKQFISKQVVFYKRIKRVFFIE 518
Query: 280 SVPKTASGKLLRRELIAK 227
++PK+ SGK+LR+EL AK
Sbjct: 519 AIPKSPSGKILRKELRAK 536
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 525,088,809
Number of Sequences: 1393205
Number of extensions: 10519522
Number of successful extensions: 35734
Number of sequences better than 10.0: 2169
Number of HSP's better than 10.0 without gapping: 30360
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 33602
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 28144814643
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
